diff --git a/app/assets/javascripts/issues/list/constants.js b/app/assets/javascripts/issues/list/constants.js
index 8c7e5e92910ead5095defe47e802b656db59f9da..3e2c923f0ac7b6cad638b2c2b7ca66f56d67a187 100644
--- a/app/assets/javascripts/issues/list/constants.js
+++ b/app/assets/javascripts/issues/list/constants.js
@@ -316,6 +316,9 @@ export const filtersMap = {
       [OPERATOR_IS]: {
         [NORMAL_FILTER]: 'source_branches[]',
       },
+      [OPERATOR_NOT]: {
+        [NORMAL_FILTER]: 'not[source_branches][]',
+      },
     },
   },
   [TOKEN_TYPE_TARGET_BRANCH]: {
diff --git a/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue b/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue
index 29e179ef2fac759fff3f4aa3493339f61d64acf1..685c36ba9598a591de3fd8f41629d88760438c9c 100644
--- a/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue
+++ b/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue
@@ -353,7 +353,6 @@ export default {
           title: TOKEN_TITLE_SOURCE_BRANCH,
           icon: 'branch',
           token: BranchToken,
-          operators: OPERATORS_IS,
           fullPath: this.fullPath,
           isProject: true,
           fetchBranches: this.fetchSourceBranches,
diff --git a/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js b/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js
index 6c9beca7b330efbf94dbfcef25eab1a0e1aaaa7e..1af59ad9a190cde8eac7ecc90dda4d4f467bd902 100644
--- a/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js
+++ b/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js
@@ -417,19 +417,31 @@ describe('Merge requests list app', () => {
             type: 'reviewer',
             value: { data: 'root', operator: OPERATOR_NOT },
           },
+          {
+            type: 'source-branch',
+            value: { data: ['branch_name'], operator: OPERATOR_NOT },
+          },
         ]);
 
         await nextTick();
 
         expect(getQueryResponseMock).toHaveBeenCalledWith(
           expect.objectContaining({
-            not: { assigneeUsernames: ['root'], reviewerUsername: 'root' },
+            not: {
+              assigneeUsernames: ['root'],
+              reviewerUsername: 'root',
+              sourceBranches: ['branch_name'],
+            },
           }),
         );
 
         expect(getCountsQueryResponseMock).toHaveBeenCalledWith(
           expect.objectContaining({
-            not: { assigneeUsernames: ['root'], reviewerUsername: 'root' },
+            not: {
+              assigneeUsernames: ['root'],
+              reviewerUsername: 'root',
+              sourceBranches: ['branch_name'],
+            },
           }),
         );
       });
@@ -446,6 +458,10 @@ describe('Merge requests list app', () => {
             type: 'reviewer',
             value: { data: 'root', operator: OPERATOR_NOT },
           },
+          {
+            type: 'source-branch',
+            value: { data: ['branch_name'], operator: OPERATOR_NOT },
+          },
         ]);
 
         await nextTick();
@@ -454,6 +470,7 @@ describe('Merge requests list app', () => {
           query: expect.objectContaining({
             'not[assignee_username][]': ['root'],
             'not[reviewer_username]': 'root',
+            'not[source_branches][]': ['branch_name'],
           }),
         });
       });