diff --git a/app/assets/javascripts/issues/list/constants.js b/app/assets/javascripts/issues/list/constants.js
index 3e2c923f0ac7b6cad638b2c2b7ca66f56d67a187..f2424d5cba3fb3f19638f387a203041f33706650 100644
--- a/app/assets/javascripts/issues/list/constants.js
+++ b/app/assets/javascripts/issues/list/constants.js
@@ -329,6 +329,9 @@ export const filtersMap = {
       [OPERATOR_IS]: {
         [NORMAL_FILTER]: 'target_branches[]',
       },
+      [OPERATOR_NOT]: {
+        [NORMAL_FILTER]: 'not[target_branches][]',
+      },
     },
   },
   [TOKEN_TYPE_TYPE]: {
diff --git a/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue b/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue
index 16d4fa3f7a153f648073c3a02010bd98bf910ee5..616c85664a538e4665e85b15925538043a8e7784 100644
--- a/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue
+++ b/app/assets/javascripts/merge_requests/list/components/merge_requests_list_app.vue
@@ -340,7 +340,6 @@ export default {
           title: TOKEN_TITLE_TARGET_BRANCH,
           icon: 'arrow-right',
           token: BranchToken,
-          operators: OPERATORS_IS,
           fullPath: this.fullPath,
           isProject: true,
           fetchBranches: this.fetchTargetBranches,
diff --git a/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js b/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js
index 1af59ad9a190cde8eac7ecc90dda4d4f467bd902..a9f6f2570ffcffba814ce136eae3fca982fe1c79 100644
--- a/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js
+++ b/spec/frontend/merge_requests/list/components/merge_requests_list_app_spec.js
@@ -421,6 +421,10 @@ describe('Merge requests list app', () => {
             type: 'source-branch',
             value: { data: ['branch_name'], operator: OPERATOR_NOT },
           },
+          {
+            type: 'target-branch',
+            value: { data: ['branch_name'], operator: OPERATOR_NOT },
+          },
         ]);
 
         await nextTick();
@@ -431,6 +435,7 @@ describe('Merge requests list app', () => {
               assigneeUsernames: ['root'],
               reviewerUsername: 'root',
               sourceBranches: ['branch_name'],
+              targetBranches: ['branch_name'],
             },
           }),
         );
@@ -441,6 +446,7 @@ describe('Merge requests list app', () => {
               assigneeUsernames: ['root'],
               reviewerUsername: 'root',
               sourceBranches: ['branch_name'],
+              targetBranches: ['branch_name'],
             },
           }),
         );
@@ -462,6 +468,10 @@ describe('Merge requests list app', () => {
             type: 'source-branch',
             value: { data: ['branch_name'], operator: OPERATOR_NOT },
           },
+          {
+            type: 'target-branch',
+            value: { data: ['branch_name'], operator: OPERATOR_NOT },
+          },
         ]);
 
         await nextTick();
@@ -471,6 +481,7 @@ describe('Merge requests list app', () => {
             'not[assignee_username][]': ['root'],
             'not[reviewer_username]': 'root',
             'not[source_branches][]': ['branch_name'],
+            'not[target_branches][]': ['branch_name'],
           }),
         });
       });