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Lasham et al., 2010 - Google Patents

A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5′ RACE and a molecular beacon probe

Lasham et al., 2010

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Document ID
18185220772386106055
Author
Lasham A
Herbert M
Coppieters ‘t Wallant N
Patel R
Feng S
Eszes M
Cao H
Reid G
Publication year
Publication venue
Nucleic acids research

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Specific detection of mRNA cleavage by 5′ RACE is the only method to confirm the knockdown of mRNA by RNA interference, but is rarely reported for in vivo studies. We have combined 5′-RNA-linker-mediated RACE (5′-RLM-RACE) with real-time PCR using a …
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    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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