Search Results for "assay"
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Showing 26 open source projects for "assay"
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CometAnalyser
CometAnalyser, for quantitative comet assay analysis.
Description: Comet assay provides an easy solution to estimate DNA damage in single cells through microscopy assessment. To obtain reproducible and reliable quantitative data, we developed an easy-to-use tool named CometAnalyser. CometAnalyser is an open-source deep-learning tool designed for the analysis of both fluorescent and silver-stained wide-field microscopy images. Once the comets are segmented and classified, several intensity/morphological features are automatically exported... -
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APIRL
Library and command line tools for Tomographic Image Reconstruction
APIRL (AR-PET Image Reconstruction Library) is a library with command line tools for tomographic image reconstruction in emission tomography. It was developed to provide an image reconstruction algorithm for the AR-PET (Argentine Positron Emission Tomography), a low cost PET scanner. The implemented algorithms are based on the well known iterative algorithms MLEM and OSEM. In addition, provides an image reconstruction algorithm for a tomographic gamma scanner for non-destructive assay... -
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Aestel
Applications for data management
"Information is data in action", and, consequently, having good quality data is essential. The AESTEL package contains two highly configurable applications for data management: A data loader and a reporting application, i.e. DataLoader and AEREA, respectively. The data loader application applies user-defined instructions to validate, process and load data. The reporting application provides a query builder and spreadsheet template designer. Both applications work with any relational data... -
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Cell Migration Assay
Cell migration is regulated by the relevant signaling molecules.
Cell migration is a fundamental process that involves the translation of cells from one location to another by adopting different motility modes, such as mesenchymal, amoeboid, or collective migration. Cell migration is essential for the development and maintenance of multicellular organisms. It also plays a key role in cell migration-mediated tissue organization, organogenesis, and homeostasis, especially in the immune response and cancer spreading which are the hotspots of clinical studies... -
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Cell proliferation is defined as the process that leads to an increase in the number of cells and the balance between cell divisions and cell loss. Cell proliferation plays an important role in regular tissue and cellular homeostasis for proper growth, development and maintenance of the organism. Moreover, abnormal cell proliferation is associated with different diseases, such as cancer. The cell proliferation assays can be used for pharmaceutical drug discovery and diagnostic purposes.
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Toxtree: Toxic Hazard Estimation
Toxicity prediction for chemical compounds
... Metabolism and metabolites prediction -Structure Alerts for the in vivo micronucleus assay in rodents (ISSMIC) -Structural Alerts for Functional Group Identification (ISSFUNC) -Structural alerts associated with covalent protein binding and DNA binding. - Ames mutagenicity Toxtree provides a plugin framework to incorporate different approaches to the estimation. Platform independent (written in Java), with the use of The Chemistry Development Kit.">
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ViralPlaque
A fast, open-source and versatile ImageJ macro for the automated dete
Plaque assay has been used for a long time to determine infectious titers. We present ViralPlaque, a fast, open-source and versatile ImageJ macro for the automated determination of viral plaque dimensions from digital images. Also, a machine learning plugin is integrated in the analysis algorithm for adaptation of ViralPlaque to the user’s needs and experimental conditions. A high correlation between manual and automated measurements of plaque dimensions was demonstrated. This macro... -
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TSA_CRAFT
Automatic command line tool for TSA data analysis
DSF is a high-throughput platform of TSA assay to screen various conditions that affect protein stability. To facilitate TSA data analysis, we developed an automatic tool "TSA-CRAFT". TSA-CRAFT was developed by integrating PERL script and Gnuplot. PERL scripts manage the entire workflow and data processing procedures of TSA data analysis. On the other hand, Gnuplot takes over the curve fitting and result presentation works. All analysed results are coded in an html file that can be easily... -
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Protein Microarray Analyser
Protein microarray data processing and normalization
The Protein Microarray Analyser software presented here includes the following tools: (1) neighbourhood background correction, (2) net intensity correction, (3) user-defined noise threshold, (4) user-defined CV threshold amongst replicates and (5) assay controls, (6) composite ‘pin-to-pin’ normalization amongst sub-arrays, and (7) ‘array-to-array’ normalization amongst whole arrays. -
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MSP-HTPrimer
A high-throughput primer design tool for DNA methylation analysis
MSP-HTPrimer is an open source, web-based high-throughput and genome-wide primer design pipeline for bisulfite-based assays (MSP, BSP and COBRA) and MSRE-PCR assay and capable of simultaneously processing hundreds to thousands of target sequences. MSP-HTPrimer takes genome-wide annotations of SNPs and repeats into consideration to design primer pairs for higher success rate. MSP-HTPrimer enables hierarchical filtering and visualisation of designed primers in UCSC genome browser for efficient... -
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sgRNAcas9
a software for designing CRISPR sgRNA and evaluating Off-Target Sites
... cleavage assay. Importantly, by identifying potential off-target sites in silico, the sgRNAcas9 allows the selection of more specific target sites and aids the identification of bona fide off-target sites, significantly facilitating the design of sgRNA for genome editing applications. Cites: Xie S, ShenB,Zhang C, Huang X, Zhang Y. sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. PLoS One. 2014,9(6):e100448. http://www.biootools.com/ -
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MSRE-HTPrimer
A high-throughput primer design tool for MSRE-assay in epigenetics
MSRE-HTPrimer is an open-source, portable, web-based and easy-to-use tool, which facilitates the design of primer pairs for epigenetic assay design and and genomic sequencing assay design. It uses a simple input and output model and can design primers for hundreds to thousands of target sequences in a single run. Moreover, it does not have any limitations on the number and size of target sequences. MSRE-HTPrimer provides significant improvements over existing solutions with following unique... -
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GCMS correction
correction of GCMS data for natural isotope abundance/peaks overlaping
The analysis of distribution of artificially labelled 13C isotopomers is generally accepted as a powerful method for the evaluation of metabolic fluxes in cells under various conditions in vivo. The primary step in such an analysis should be not only a separation between natural and artificial labelling of metabolites that both contribute to the measured mass spectrometry data, but also correction for “impurities” of the assay media that give peaks overlapping with the desired pattern. Whereas... -
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FPF_predict
Fine Particle Fraction (FPF) predictor
Application implements models described by classical mathematical equation for in vitro deposition prediction based on characteristics of formulation and assay conditions. This work was funded by Poland-Singapore bilateral cooperation project no 2/3/POL-SIN/2012. Published article: https://www.dovepress.com/empirical-modeling-of-the-fine-particle-fraction-fornbspcarrier-based--peer-reviewed-fulltext-article-IJN -
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CEMAsuite
Consensus/Degenerate Primer Design Application
... results in various formats. The goal of CEMAsuite is to aid in the design of minimum-degeneracy primer sets which are robust enough for the assay at hand, while retaining as much specificity and product homology as possible. admin: cemasuite@gmail.com -
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simulate_pcr
PCR target prediction software
Assessing primer specificity and predicting both desired and off-target amplification products is an essential step for robust PCR assay design. This script predicts potential polymerase chain reaction (PCR) amplicons in a large sequence database such as NCBI nt from either singleplex or a large multiplexed set of primers, allowing degenerate primer and probe bases, with target mismatch tolerance and amplicon length range to be set by the user. The PCR amplicon simulation code also annotates... -
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CaspLab - Comet Assay Software Project Lab is a cross-platform image analysis software to measure level of DNA damage in SCGE (Single Cell Gel Electrophoresis) method called also comet assay method.
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The Investigation/Study/Assay (ISA) Infrastructure is the first pilot-stage freely available software suite that 1. assists in the reporting and local management of experimental metadata and facilitates submission to international public repositories.
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ClinStudyWeb is designed to provide a flexible infrastructure for managing patient and assay data from clinical studies. It uses a plugin system for study-specific web forms and arbitrarily complex test classifiers, and supports XML import/export.
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Arduino for Circadian Behavioral Assay
Circadian behaviral assay software for Arduino 1.0
The Arduino routines (.ino) control light-on timing under various entrainment schemes and measures general movements using PIR and the light level using CdS in three animal chambers. Requires Arduino 1.0 and Arduino hardwares (Uno or Mega). 1. _3ChambersEPLPDD.ino: 12-12 LD to long photoperiod 2. _3ChambersTCycle.ino: 12-12 LD to T=23/T=25 cycles (Note: A critical error has been found in _3ChambersTCycle.ino. It will not be available until the error is fixed. 14-SEP-2012) -
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mPSQed
Alignment editor and multiplex pyrosequencing assay designer
Molecular-based diagnostic assays are the gold standard for infectious diseases today, since they allow a rapid and sensitive identification and typing of various pathogens. While PCR can be designed to be specific for a certain pathogen, a subsequent sequence analysis is frequently required for confirmation or typing. The design of appropriate PCR-based assays is a complex task, especially when conserved discriminating polymorphisms are rare or if the number of types which need to be... -
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This software uses randomly distributed airy disks along a user defined length to imitate the images a microscope will produce from a gliding motility assay.
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Jyrax is a Java-based, embeddable DAS/2 client designed to work with the 'assay' portion of the evolving DAS/2 specification. Jyrax can query a DAS/2 server and select microarray or other experimental data for retrieval.
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PACLIMS (Phenotype Assay and Characterization Laboratory Information Management System) is a web-based, open source software system for tracking and data capture from high throughput mutational screens. Click on the Home Page Link above for test DB