[go: up one dir, main page]

Dunham, 2025 - Google Patents

Enhancing characterization of intact proteins by UVPD mass spectrometry and charge reduction reactions

Dunham, 2025

View PDF
Document ID
4004602438678016634
Author
Dunham S
Publication year

External Links

Snippet

Access to high resolution mass spectrometers and high energy modes of activation such as electron-and photon-based modalities have enabled wider adoption of top-down methodologies, or strategies that allow the study of intact proteins. However, interpretation of …
Continue reading at repositories.lib.utexas.edu (PDF) (other versions)

Classifications

    • H—ELECTRICITY
    • H01—BASIC ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometer or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/34—Dynamic spectrometers
    • H01J49/42—Stability-of-path spectrometers, e.g. monopole, quadrupole, multipole, farvitrons
    • H—ELECTRICITY
    • H01—BASIC ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometer or separator tubes
    • H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
    • H01J49/0045—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction
    • H01J49/0054—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction by an electron beam, e.g. electron impact dissociation, electron capture dissociation
    • H—ELECTRICITY
    • H01—BASIC ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometer or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
    • H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]
    • H—ELECTRICITY
    • H01—BASIC ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometer or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • H—ELECTRICITY
    • H01—BASIC ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometer or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0031—Step by step routines describing the use of the apparatus

Similar Documents

Publication Publication Date Title
Rolland et al. Approaches to heterogeneity in native mass spectrometry
Foreman et al. Recent developments in gas-phase ion/ion reactions for analytical mass spectrometry
Qi et al. Electron‐based fragmentation methods in mass spectrometry: an overview
Banerjee et al. Electrospray ionization mass spectrometry: a technique to access the information beyond the molecular weight of the analyte
Sannes‐Lowery et al. Multipole storage assisted dissociation, a novel in‐source dissociation technique for electrospray ionization generated ions
Inutan et al. Laserspray ionization, a new method for protein analysis directly from tissue at atmospheric pressure with ultrahigh mass resolution and electron transfer dissociation
Bourgoin‐Voillard et al. Top‐down tandem mass spectrometry on RN ase A and B using a Q h/FT‐ICR hybrid mass spectrometer
Martin et al. Analysis of high mass peptides using a novel matrix‐assisted laser desorption/ionisation quadrupole ion trap time‐of‐flight mass spectrometer
JP5727503B2 (en) Multiple tandem mass spectrometry
Gross et al. Protein and peptide mass spectrometry in drug discovery
US8153961B2 (en) Methods for acquisition and deductive analysis of mixed fragment peptide mass spectra
US10495647B2 (en) Analysis of complex biological matrices through targeting and advanced precursor and product ion alignment
US8890062B2 (en) Differential mobility spectrometer and methods thereof
Dunham Enhancing characterization of intact proteins by UVPD mass spectrometry and charge reduction reactions
Dunham Enhancing the characterization of intact proteins by ultraviolet photodissociation mass spectrometry
Sanders Development of mass spectrometry and ion mobility methods and instrumentation for the characterization of proteins from primary sequence to higher order structure
Bashyal Characterization of protein post-translational modifications using 193 nm ultraviolet photodissociation mass spectrometry
Pitteri et al. Charge-state dependent dissociation of a trypsin/inhibitor complex via ion trap collisional activation
Dunham et al. Impact of charge reduction on sequence coverage of proteins by 193 nm ultraviolet photodissociation
Carrick Development of an Electrostatic Linear Ion Trap as a Standalone Tandem Mass Spectrometer
Garcia et al. Top-Down Mass Spectrometry of a Clinical Antibody Light Chain Using the Omnitrap-Orbitrap-Booster Platform
Peterkin ION Isolation and Gas-Phase Charge Reduction for the Analysis of Protein Mixtures
Peters-Clarke background and introduction
Bhanot Development and Application of a Quadrupole Time-of-Flight Mass Spectrometer for the Analysis of Synthetic Polymers, Proteins, and Protein Complexes
Cook Metastable Atom-Activated Dissociation (MAD): A Novel Dissociation Method Employed within a Quadrupole Ion Trap Mass Spectrometer