[go: up one dir, main page]

Wong et al., 2018 - Google Patents

Design of modular polyhydroxyalkanoate scaffolds for protein immobilization by directed ligation

Wong et al., 2018

Document ID
5674946655917968378
Author
Wong J
Rehm B
Publication year
Publication venue
Biomacromolecules

External Links

Snippet

In vivo-assembled polyhydroxyalkanoate (PHA) particles have been successfully bioengineered to display foreign protein functions toward high-value applications in medicine and industry. To further expand the design space of PHA particles toward …
Continue reading at pubs.acs.org (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells being immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells being immobilised on or in an organic carrier entrapped within the carrier, e.g. gel, hollow fibre
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells being immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells being immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells being immobilised on or in an organic carrier carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals

Similar Documents

Publication Publication Date Title
Wong et al. Design of modular polyhydroxyalkanoate scaffolds for protein immobilization by directed ligation
Uchida et al. Modular self-assembly of protein cage lattices for multistep catalysis
Dedisch et al. Matter‐tag: A universal immobilization platform for enzymes on polymers, metals, and silicon‐based materials
Olcucu et al. Emerging solutions for in vivo biocatalyst immobilization: tailor-made catalysts for industrial biocatalysis
Alves et al. Bacterial nanobioreactors–directing enzyme packaging into bacterial outer membrane vesicles
Roessl et al. Carrier-free immobilized enzymes for biocatalysis
Zhang et al. Developing a protein scaffolding system for rapid enzyme immobilization and optimization of enzyme functions for biocatalysis
Ross et al. Decorating self-assembled peptide cages with proteins
Caparco et al. Protein mediated enzyme immobilization
Gilbert et al. Extracellular self-assembly of functional and tunable protein conjugates from Bacillus subtilis
Wong et al. Bioengineered polyhydroxyalkanoates as immobilized enzyme scaffolds for industrial applications
Raliski et al. Site-specific protein immobilization using unnatural amino acids
US9815871B2 (en) Genetic reprogramming of bacterial biofilms
Yin et al. Self-assembled multimeric-enzyme nanoreactor for robust and efficient biocatalysis
Wong et al. Covalent functionalization of bioengineered polyhydroxyalkanoate spheres directed by specific protein-protein interactions
Kim et al. Stability-controllable self-immobilization of carbonic anhydrase fused with a silica-binding tag onto diatom biosilica for enzymatic CO2 capture and utilization
Chen et al. Programming an orthogonal self-assembling protein cascade based on reactive peptide–protein pairs for in vitro enzymatic trehalose production
Wang et al. CipA and CipB as scaffolds to organize proteins into crystalline inclusions
Hooks et al. Polyhydroyxalkanoate synthase fusions as a strategy for oriented enzyme immobilisation
Liu et al. Plug-and-play functionalization of protein–polymer conjugates for tunable catalysis enabled by genetically encoded “click” chemistry
Bulos et al. Design of a superpositively charged enzyme: human carbonic anhydrase II variant with ferritin encapsulation and immobilization
Tschiggerl et al. Exploitation of the S-layer self-assembly system for site directed immobilization of enzymes demonstrated for an extremophilic laminarinase from Pyrococcus furiosus
Li et al. Site-specific and high-loading immobilization of proteins by using cohesin–dockerin and CBM–cellulose interactions
Evert et al. Catalytically active bioseparation resin utilizing a covalent intermediate for tagless protein purification
Fedeson et al. Cyanobacterial surface display system mediates engineered interspecies and abiotic binding