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Beck et al., 1988 - Google Patents

Efficient production of active human manganese superoxide dismutase in Escherichia coli

Beck et al., 1988

Document ID
5687496262630716618
Author
Beck Y
Bartfeld D
Yavin Z
Levanon A
Gorecki M
Hartman J
Publication year
Publication venue
Bio/Technology

External Links

Snippet

Human manganese superoxide dismutase (hMnSOD) was expressed in Escherichia coli under regulation of the PL promoter and c II ribosomal binding site (rbs), derived from bacteriophage λ. Production of hMnSOD was induced upon temperature shift from 32° C to …
Continue reading at www.nature.com (other versions)

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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