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Feliu et al., 1994 - Google Patents

An optimized ultrasonication protocol for bacterial cell disruption and recovery of β-galactosidase fusion proteins

Feliu et al., 1994

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Document ID
6622676881486646102
Author
Feliu J
Villaverde A
Publication year
Publication venue
Biotechnology techniques

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In this work, we have developed and optimized an ultrasonication protocol for Escherichia coli recombinant cells, adapted to laboratory-scale release of β-galactosidase fusion proteins. After a single sonication treatment of 15 minutes, about 30% of recombinant protein …
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    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
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