Keomanee-Dizon et al., 2020 - Google Patents
A versatile, multi-laser twin-microscope system for light-sheet imagingKeomanee-Dizon et al., 2020
View HTML- Document ID
- 723395310293073619
- Author
- Keomanee-Dizon K
- Fraser S
- Truong T
- Publication year
- Publication venue
- Review of Scientific Instruments
External Links
Snippet
Light-sheet microscopy offers faster imaging and reduced phototoxicity in comparison to conventional point-scanning microscopy, making it a preferred technique for imaging biological dynamics for durations of hours or days. Such extended imaging sessions pose a …
- 238000003384 imaging method 0 title abstract description 72
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultra-violet illumination; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/10—Condensers affording dark-field illumination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N2021/653—Coherent methods [CARS]
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0012—Surgical microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Keller et al. | Light sheet microscopy of living or cleared specimens | |
| Keomanee-Dizon et al. | A versatile, multi-laser twin-microscope system for light-sheet imaging | |
| Gao et al. | 3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy | |
| Olarte et al. | Light-sheet microscopy: a tutorial | |
| Chen et al. | Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution | |
| Keller | In vivo imaging of zebrafish embryogenesis | |
| Diaspro et al. | Multi-photon excitation microscopy | |
| Greger et al. | Basic building units and properties of a fluorescence single plane illumination microscope | |
| Reynaud et al. | Light sheet‐based fluorescence microscopy: more dimensions, more photons, and less photodamage | |
| US8994807B2 (en) | Microscopy system and method for creating three dimensional images using probe molecules | |
| US8575570B2 (en) | Simultaneous orthogonal light sheet microscopy and computed optical tomography | |
| Elisa et al. | Technical implementations of light sheet microscopy | |
| Tomer et al. | Shedding light on the system: studying embryonic development with light sheet microscopy | |
| Follain et al. | Seeing is believing–multi-scale spatio-temporal imaging towards in vivo cell biology | |
| US10595770B2 (en) | Imaging platform based on nonlinear optical microscopy for rapid scanning large areas of tissue | |
| So et al. | High-throughput nonlinear optical microscopy | |
| Gavryusev et al. | Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector | |
| Swoger et al. | Light-sheet-based fluorescence microscopy for three-dimensional imaging of biological samples | |
| WO2016020684A1 (en) | Multiplexed optical tomography | |
| Liu et al. | VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy | |
| WO2013176549A1 (en) | Optical apparatus for multiple points of view three-dimensional microscopy and method | |
| Tserevelakis et al. | Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements | |
| Wu et al. | Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning | |
| Shakhi et al. | An in-house constructed dual channel confocal fluorescence microscope for biomolecular imaging | |
| Keomanee-Dizon et al. | A versatile twin-microscope system for light-sheet imaging |