Fischer et al., 2012 - Google Patents
Nanoparticle mediated protein separation in aqueous micellar two-phase systemsFischer et al., 2012
- Document ID
- 7402316028487935234
- Author
- Fischer I
- Franzreb M
- Publication year
- Publication venue
- Solvent Extraction and Ion Exchange
External Links
Snippet
Magnetic nanoparticles with cation exchange functionality (MNCX) are combined with an Aqueous Micellar Two-Phase System (AMTPS) based on the nonionic surfactant Eumulgin ES for the purpose of protein separation. As proof of principle the positively charged protein …
- 102000004169 proteins and genes 0 title abstract description 120
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dainiak et al. | Cell chromatography: Separation of different microbial cells using IMAC supermacroporous monolithic columns | |
| Safarik et al. | Magnetic techniques for the isolation and purification of proteins and peptides | |
| Tarrant et al. | Host cell protein adsorption characteristics during protein A chromatography | |
| Becker et al. | Protein separation with magnetic adsorbents in micellar aqueous two-phase systems | |
| JP6385374B2 (en) | Substances and methods for removing endotoxins from protein formulations | |
| US8158007B2 (en) | Method for magnetically supported extraction | |
| Dhadge et al. | Magnetic aqueous two phase fishing: a hybrid process technology for antibody purification | |
| Gagnon et al. | Chromatographic behavior of IgM: DNA complexes | |
| Nian et al. | Void exclusion of antibodies by grafted-ligand porous particle anion exchangers | |
| Morrison et al. | Purification of monomeric mAb from associated aggregates using selective desorption chromatography in hydroxyapatite systems | |
| Muthukumar et al. | High throughput process development (HTPD) platform for membrane chromatography | |
| Ceylan et al. | Novel adsorbent for DNA adsorption: Fe3+-attached sporopollenin particles embedded composite cryogels | |
| Hosseini et al. | Large-scale purification of recombinant hepatitis B surface antigen from Pichia pastoris with non-affinity chromatographic methods as a substitute to immunoaffinity chromatography | |
| Jeffery | Expression, solubilization, and purification of bacterial membrane proteins | |
| Fischer et al. | Nanoparticle mediated protein separation in aqueous micellar two-phase systems | |
| Wang et al. | Antibody purification from CHO cell supernatant using new multimodal membranes | |
| CN104136121A (en) | REDUCING pH EXCURSIONS IN ION EXCHANGE CHROMATOGRAPHY | |
| Nilsson et al. | Hydrophobic interaction capillary electrochromatography of protein mutants. Use of lipid-based liquid crystalline nanoparticles as pseudostationary phase | |
| Xia et al. | Influences of ligand structure and pH on the adsorption with hydrophobic charge induction adsorbents: a case study of antibody IgY | |
| Faude et al. | Fast determination of conditions for maximum dynamic capacity in cation-exchange chromatography of human monoclonal antibodies | |
| Walch et al. | Continuous desalting of refolded protein solution improves capturing in ion exchange chromatography: a seamless process | |
| Wu et al. | Evaluation of a PEG/hydroxypropyl starch aqueous two‐phase system for the separation of monoclonal antibodies from cell culture supernatant | |
| JP2014523405A5 (en) | ||
| Xia et al. | Purification of immunoglobulin of egg yolk with hydrophobic charge induction chromatography: Comparison of operation modes with packed bed and expanded bed | |
| Yan et al. | Protein adsorption behavior and immunoglobulin separation with a mixed‐mode resin based on p‐aminohippuric acid |