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Klomklao et al., 2006 - Google Patents

Proteolytic degradation of sardine (Sardinella gibbosa) proteins by trypsin from skipjack tuna (Katsuwonus pelamis) spleen

Klomklao et al., 2006

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Document ID
7968767803889810196
Author
Klomklao S
Benjakul S
Visessanguan W
Kishimura H
Simpson B
Publication year
Publication venue
Food Chemistry

External Links

Snippet

Trypsin from the spleen of skipjack tuna (Katsuwonus pelamis) was purified by ammonium sulfate precipitation and a series of chromatographies, including Sephacryl S-100 and benzamidine-Sepharose 4 fast flow (high sub). The enzyme was purified 22.3-fold with a …
Continue reading at eprints.lib.hokudai.ac.jp (PDF) (other versions)

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases Endopeptidases (3.4.21-3.4.25) derived from bacteria
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/30Working-up of proteins for foodstuffs by hydrolysis

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