[go: up one dir, main page]

Wang et al., 1993 - Google Patents

Phorbol 12-myristate 13-acetate-induced phosphorylation of Op18 in Jurkat T cells. Identification of phosphorylation sites by matrix-assisted laser desorption …

Wang et al., 1993

View PDF
Document ID
860994332256900172
Author
Wang Y
Liao P
Allison J
Gage D
Andrews P
Lubman D
Hanash S
Strahler J
Publication year
Publication venue
Journal of Biological Chemistry

External Links

Snippet

Op18 is a widely expressed, cell cycle-regulated, phosphoprotein involved in signal transduction of a variety of stimuli. In actively proliferating Jurkat T cells which express Op18 at high level, phorbol 12-myristate 13-acetate (PMA) treatment induces a rapid increase in …
Continue reading at www.jbc.org (PDF) (other versions)

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes

Similar Documents

Publication Publication Date Title
Wang et al. Phorbol 12-myristate 13-acetate-induced phosphorylation of Op18 in Jurkat T cells. Identification of phosphorylation sites by matrix-assisted laser desorption ionization mass spectrometry
Larsen et al. Mass spectrometric identification of proteins and characterization of their post-translational modifications in proteome analysis
Liao et al. An approach to locate phosphorylation sites in a phosphoprotein: mass mapping by combining specific enzymatic degradation with matrix-assisted laser desorption/ionization mass spectrometry
Jensen et al. Mass spectrometric identification and microcharacterization of proteins from electrophoretic gels: strategies and applications
Annan et al. The essential role of mass spectrometry in characterizing protein structure: mapping posttranslational modifications
Hamler et al. A two‐dimensional liquid‐phase separation method coupled with mass spectrometry for proteomic studies of breast cancer and biomarker identification
US6642059B2 (en) Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy
US5869240A (en) Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry
Parker et al. The phosphorylation of eukaryotic ribosomal protein S6 by protein kinase C
EP2419739B1 (en) Method for quantifying modified peptides
Ramachandran et al. Phosphorylation of high-mobility-group proteins by the calcium-phospholipid-dependent protein kinase and the cyclic AMP-dependent protein kinase.
Abraham et al. Post-translational modification of p53 protein in response to ionizing radiation analyzed by mass spectrometry
Besant et al. Detection and analysis of protein histidine phosphorylation
Stover et al. Cdc2-mediated modulation of pp60c-src activity.
EGGERT et al. Identification of novel phosphorylation sites in murine A‐type lamins
Caprioli Analysis of biochemical reactions with molecular specificity using fast atom bombardment mass spectrometry
Affolter et al. Evaluation of two-dimensional phosphopeptide maps by electrospray ionization mass spectrometry of recovered peptides
US4668624A (en) Protein translation method
Labdon et al. Analysis of phosphoprotein p19 by liquid chromatography/mass spectrometry. Identification of two proline-directed serine phosphorylation sites and a blocked amino terminus.
Merrick et al. Site-specific phosphorylation of human p53 protein determined by mass spectrometry
Kozmin et al. A direct introduction of 18O isotopes into peptides and proteins for quantitative mass spectroscopy analysis
SITES et al. Phorbol 12-Myristate 13-Acetate-induced Phosphorylation of Op18 in Jurkat T Cells
Singh et al. Phosphorylation and activation of rabbit skeletal muscle phosphorylase kinase by a cyclic nucleotide-and Ca2+-independent protein kinase.
Konrad et al. Purification of the O-glycosylated protein p135 and identification as O-GlcNAc transferase
Sefton [20] Measurement of stoichiometry of protein phosphorylation by biosynthetic labeling