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Wolfe et al., 2010 - Google Patents

A herpes simplex virus vector system for expression of complex cellular cDNA libraries

Wolfe et al., 2010

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Document ID
8805527003772668729
Author
Wolfe D
Craft A
Cohen J
Glorioso J
Publication year
Publication venue
Journal of virology

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Snippet

Viral vector-based gene expression libraries from normal or diseased tissues offer opportunities to interrogate cellular functions that influence or participate directly in specific biological processes. Here we report the creation and characterization of a herpes simplex …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
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    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/09—Recombinant DNA-technology
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