[go: up one dir, main page]

Baumgart et al., 2008 - Google Patents

Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53a cells

Baumgart et al., 2008

View HTML @Full View
Document ID
9525230618596027089
Author
Baumgart J
Bintig W
Ngezahayo A
Willenbrock S
Escobar H
Ertmer W
Lubatschowski H
Heisterkamp A
Publication year
Publication venue
Optics express

External Links

Snippet

Opto-perforation is an interesting alternative to conventional techniques for gene transfer into living cells. The cell membrane is perforated by femtosecond (fs) laser pulses, in order to induce an uptake of macromolecules eg DNA. In this study, we successfully transfected a …
Continue reading at opg.optica.org (HTML) (other versions)

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458Fluorescence microscopy
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"

Similar Documents

Publication Publication Date Title
Baumgart et al. Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53a cells
Spahn et al. Whole-cell, 3D, and multicolor STED imaging with exchangeable fluorophores
Stevenson et al. Femtosecond optical transfection of cells: viability and efficiency
König et al. Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes
Waleed et al. Single-cell optoporation and transfection using femtosecond laser and optical tweezers
Jess et al. Dual beam fibre trap for Raman microspectroscopy of single cells
Mitra et al. Analysis of mitochondrial dynamics and functions using imaging approaches
Belashov et al. Digital holographic microscopy in label-free analysis of cultured cells’ response to photodynamic treatment
Supatto et al. In vivo modulation of morphogenetic movements in Drosophila embryos with femtosecond laser pulses
Davidson et al. Toxicity minimized cryoprotectant addition and removal procedures for adherent endothelial cells
Tang et al. NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers
Bader et al. Imaging of protein cluster sizes by means of confocal time-gated fluorescence anisotropy microscopy
Liu et al. Two-photon fluorescence excitation in continuous-wave infrared optical tweezers
Haslehurst et al. Fast volume-scanning light sheet microscopy reveals transient neuronal events
Ferraro-Gideon et al. Measurements of forces produced by the mitotic spindle using optical tweezers
LaFountain Jr et al. Microtubule flux mediates poleward motion of acentric chromosome fragments during meiosis in insect spermatocytes
Stevenson et al. Optically guided neuronal growth at near infrared wavelengths
He et al. Manipulation of cellular light from green fluorescent protein by a femtosecond laser
Engelbrecht et al. Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM)
Biskup et al. Interaction of PSD-95 with potassium channels visualized by fluorescence lifetime-based resonance energy transfer imaging
Watanabe et al. Single-organelle tracking by two-photon conversion
Sacconi et al. Optical recording of electrical activity in intact neuronal networks with random access second-harmonic generation microscopy
Gang et al. Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging
He et al. Targeted photoporation and transfection in human HepG2 cells by a fiber femtosecond laser at 1554 nm
Zhu et al. Molecular response of mitochondria to a short-duration femtosecond-laser stimulation