[go: up one dir, main page]

Rommelaere et al., 2004 - Google Patents

A method for rapidly screening functionality of actin mutants and tagged actins

Rommelaere et al., 2004

View PDF
Document ID
11839382395883360789
Author
Rommelaere H
Waterschoot D
Neirynck K
Vandekerckhove J
Ampe C
Publication year
Publication venue
Biological procedures online

External Links

Snippet

Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidly screen the folding capacity …
Continue reading at pmc.ncbi.nlm.nih.gov (PDF) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Similar Documents

Publication Publication Date Title
Strahm et al. The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG‐nucleoporin Rip1p, the DEAD‐box protein Rat8p/Dbp5p and a new protein Ymr255p
Kilmartin Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication
Clarkson et al. Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran
US7927813B2 (en) Peptides which interact with anti-apoptotic members of the Bcl-2 protein family, and uses
Sharma et al. Function of SSA subfamily of Hsp70 within and across species varies widely in complementing Saccharomyces cerevisiae cell growth and prion propagation
Rommelaere et al. A method for rapidly screening functionality of actin mutants and tagged actins
Fourati et al. A highly conserved region essential for NMD in the Upf2 N-terminal domain
US20090099072A1 (en) Motif of the Beclin Protein Which Interacts with Anti-Apoptotic Members of the Bcl-2 Protein Family, and Uses
Li et al. The IQGAP Iqg1 is a regulatory target of CDK for cytokinesis in Candida albicans
Sato et al. Ribosome-associated protein LBP/p40 binds to S21 protein of 40S ribosome: analysis using a yeast two-hybrid system
MXPA00011760A (en) A method of modulating the efficiency of translation termination and degradation of aberrant mrna involving a surveillance complex comprising human upf1p, eucaryotic release factor 1 and eucaryotic release factor 3.
Mayerhofer et al. Two splice variants of human PEX19 exhibit distinct functions in peroxisomal assembly
Akkari et al. Production of human skeletal α-actin proteins by the baculovirus expression system
WO2016162385A1 (en) Bacterial system for the identification of amyloidogenic peptides and the screening of inhibitors of amyloidosis
Rommelaere et al. Structural plasticity of functional actin: pictures of actin binding protein and polymer interfaces
JP2006515159A (en) MK2 interacting protein
AU2018352986A1 (en) Prenylation assay
EP4112728A1 (en) Method for identifying target-binding peptides
Nam et al. DNA light-strand preferential recognition of human mitochondria transcription termination factor mTERF
Hara et al. Prion domain interaction responsible for species discrimination in yeast [PSI+] transmission
Bertrand et al. Folding, stability and polymerization properties of FtsZ chimeras with inserted tubulin loops involved in the interaction with the cytosolic chaperonin CCT and in microtubule formation
Emran et al. A role for Yin Yang-1 (YY1) in the assembly of snRNA transcription complexes
WO2005003160A1 (en) Actin mutants
US7459434B2 (en) Peptide interacting with anti-apoptotic proteins of the Bcl-2 family
Nickens et al. The biochemical activities of the Saccharomyces cerevisiae Pif1 helicase are regulated by its N