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Herskowitz et al., 1991 - Google Patents

[8] Putting the HO gene to work: Practical uses for mating-type switching

Herskowitz et al., 1991

Document ID
190437274686117098
Author
Herskowitz I
Jensen R
Publication year
Publication venue
Methods in enzymology

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Publisher Summary This chapter presents the uses of the cloned HO gene, its target site (the Y/Z site), and the process of mating-type interconversion. There are many cases in which it is useful to have both MATa and MATα versions of a given strain. This can be done by …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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