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Hendry, 1995 - Google Patents

Shuttling of a functional cre recombinase gene into mammalian cells using a retroviral vector

Hendry, 1995

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Document ID
1978453651165952235
Author
Hendry J
Publication year

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Cre recombinase of bacteriophage PI has become a useful tool for molecular biological studies in vivo as well as in vitro. The Cre protein is an essential part of a system which catalyzes recombination between specific DNA sequence repeats called loxP sites. This …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N2710/00011—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA Viruses dsDNA Viruses
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    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
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