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Shibata et al., 2001 - Google Patents

Cloning full-length, cap-trapper-selected cDNAs by using the single-strand linker ligation method

Shibata et al., 2001

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Document ID
1978511673413124929
Author
Shibata Y
Carninci P
Watahiki A
Shiraki T
Konno H
Muramatsu M
Hayashizaki Y
Publication year
Publication venue
Biotechniques

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Snippet

We have developed the single-strand linker ligation method (SSLLM), which uses DNA ligase to add a dsDNA linker to singlestranded (ss) full-length cDNA. The linkers have random 6-bp (dN6 or dGN5) 3′ overhangs that can ligate to any cDNA sequence, thereby …
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