[go: up one dir, main page]

Cady et al., 2004 - Google Patents

A microchip-based DNA purification and real-time PCR biosensor for bacterial detection

Cady et al., 2004

Document ID
3794329546697151315
Author
Cady N
Stelick S
Kunnavakkam M
Liu Y
Batt C
Publication year
Publication venue
SENSORS, 2004 IEEE

External Links

Snippet

A miniaturized, fully-automated, PCR (polymerase chain reaction)-based detection system has been developed for the rapid detection of bacteria. Monolithic DNA purification/real-time PCR silicon chips were fabricated and tested for their ability to purify and detect the …
Continue reading at ieeexplore.ieee.org (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Hybridisation probes
    • C12Q1/6888—Hybridisation probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated micro-fluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B01L2300/1866—Microwaves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means

Similar Documents

Publication Publication Date Title
Cady et al. Real-time PCR detection of Listeria monocytogenes using an integrated microfluidics platform
US12123052B2 (en) Methods and apparatus for sequential amplification reactions
Sun et al. A lab-on-a-chip system with integrated sample preparation and loop-mediated isothermal amplification for rapid and quantitative detection of Salmonella spp. in food samples
CN110452808B (en) Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification
US20080125330A1 (en) Real-Time Pcr Detection of Microorganisms Using an Integrated Microfluidics Platform
CN101203312B (en) Cartridges, systems and methods for automated medical diagnosis
AU2022263544A1 (en) Selectively vented biological assay devices and associated methods
Borysiak et al. NAIL: Nucleic Acid detection using Isotachophoresis and Loop-mediated isothermal amplification
JP2024028879A (en) Methods and systems for nucleic acid analysis and quantification
Chang et al. Rapid isolation and detection of aquaculture pathogens in an integrated microfluidic system using loop-mediated isothermal amplification
Chang et al. Detection of viruses directly from the fresh leaves of a Phalaenopsis orchid using a microfluidic system
Fu et al. Integrated paper-based detection chip with nucleic acid extraction and amplification for automatic and sensitive pathogen detection
Jin et al. Rapid detection of foodborne bacterial pathogens using visual high‐throughput microfluidic chip
CA2734131A1 (en) Temperature controlled nucleic-acid detection method suitable for practice in a closed-system
Cady et al. A microchip-based DNA purification and real-time PCR biosensor for bacterial detection
Shi et al. Real-time PCR of single bacterial cells on an array of adhering droplets
Dignan et al. A novel method for inward fluid displacement in centrifugal microdevices for highly integrated nucleic acid processing with long-term reagent storage
Zhong et al. iso-μmGene: an isothermal amplification-based portable microfluidic system for simple, reliable and flexibly multiplexed genetic identification and quantification
Kwon et al. Fully automated molecular diagnosis by a novel cartridge-based platform
Cady et al. Rapid Detection of Bacillus anthracis in a Microchip-based Real-time PCR Biosensor
Liang et al. LampPort: a handheld digital microfluidic device for loop-mediated isothermal amplification (LAMP)
Hlawatsch et al. From microfluidic modules to an integrated Lab-on-a-chip system for the detection of Francisella tularensis
Shah Mukim Uddin et al. Optimization of the Amplicons Detection System of Loop-Mediated Isothermal Amplification on Microfluidic Compact Disk
Sureshkumar et al. An integrated and self-regulating microfluidic device for real-time multiplex detection of Escherichia coli O157: H7 and Salmonella enterica sp.
Fukuba et al. Development of miniaturized in situ analysis devices for biological and chemical oceanography