[go: up one dir, main page]

MX2020004343A - Métodos y composiciones para mejorar microbios modificados que fijan nitrógeno. - Google Patents

Métodos y composiciones para mejorar microbios modificados que fijan nitrógeno.

Info

Publication number
MX2020004343A
MX2020004343A MX2020004343A MX2020004343A MX2020004343A MX 2020004343 A MX2020004343 A MX 2020004343A MX 2020004343 A MX2020004343 A MX 2020004343A MX 2020004343 A MX2020004343 A MX 2020004343A MX 2020004343 A MX2020004343 A MX 2020004343A
Authority
MX
Mexico
Prior art keywords
methods
compositions
fix nitrogen
engineered microbes
improving
Prior art date
Application number
MX2020004343A
Other languages
English (en)
Inventor
Alvin Tamsir
Sarah Bloch
Douglas Higgings
Original Assignee
Pivot Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pivot Bio Inc filed Critical Pivot Bio Inc
Publication of MX2020004343A publication Critical patent/MX2020004343A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/26Klebsiella (G)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223Vegetative tissue-specific promoters
    • C12N15/8227Root-specific
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0095Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y118/00Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/06Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with dinitrogen as acceptor (1.18.6)
    • C12Y118/06001Nitrogenase (1.18.6.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03012Trehalose-phosphatase (3.1.3.12)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/011414-Alpha-D-{(1->4)-alpha-D-glucano} trehalose trehalohydrolase (3.2.1.141)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/34Vector systems having a special element relevant for transcription being a transcription initiation element
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/22Klebsiella

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Ecology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Forests & Forestry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan métodos y sistemas para generar y utilizar una composición bacteriana que comprende al menos una cepa bacteriana genéticamente modificada que fija nitrógeno atmosférico en un sistema agrícola que ha sido fertilizado con más de 20 libras de nitrógneo por acre.
MX2020004343A 2017-10-25 2018-10-23 Métodos y composiciones para mejorar microbios modificados que fijan nitrógeno. MX2020004343A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762577148P 2017-10-25 2017-10-25
PCT/US2018/057174 WO2019084059A2 (en) 2017-10-25 2018-10-23 METHODS AND COMPOSITIONS FOR ENHANCING GENETICALLY MODIFIED MICROBES THAT FIX NITROGEN

Publications (1)

Publication Number Publication Date
MX2020004343A true MX2020004343A (es) 2021-01-08

Family

ID=66247426

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020004343A MX2020004343A (es) 2017-10-25 2018-10-23 Métodos y composiciones para mejorar microbios modificados que fijan nitrógeno.

Country Status (12)

Country Link
US (2) US11993778B2 (es)
EP (1) EP3701040A4 (es)
JP (2) JP7420712B2 (es)
KR (1) KR20200088342A (es)
CN (2) CN120041362A (es)
AU (2) AU2018354221B2 (es)
BR (1) BR112020008035A2 (es)
CA (1) CA3079955A1 (es)
MX (1) MX2020004343A (es)
PH (1) PH12020550484A1 (es)
RU (1) RU2020116764A (es)
WO (1) WO2019084059A2 (es)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
KR102197507B1 (ko) 2015-07-13 2020-12-31 피벗 바이오, 인크. 식물 형질 개선을 위한 방법 및 조성물
WO2017062412A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
CN116904333A (zh) 2017-01-12 2023-10-20 皮沃特生物公司 用于改良植物性状的方法及组合物
KR20200044015A (ko) * 2017-08-09 2020-04-28 피벗 바이오, 인크. 유전자조작 미생물을 개선하기 위한 방법 및 조성물
AU2018354338B2 (en) 2017-10-25 2023-10-26 Pivot Bio, Inc. Gene targets for nitrogen fixation targeting for improving plant traits
EP3701040A4 (en) 2017-10-25 2021-08-25 Pivot Bio, Inc. METHODS AND COMPOSITIONS FOR IMPROVING GENETICALLY MODIFIED MICROBES THAT BIND NITROGEN
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
EP3814302A4 (en) 2018-06-27 2022-06-29 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
WO2020014498A1 (en) 2018-07-11 2020-01-16 Pivot Bio, Inc. Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes
US12421519B2 (en) 2019-01-07 2025-09-23 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
US12281299B2 (en) 2019-03-19 2025-04-22 Massachusetts Institute Of Technology Control of nitrogen fixation in rhizobia that associate with cereals
CN110331153B (zh) * 2019-06-24 2021-04-30 浙江工业大学 一种克吕沃尔氏菌酪氨酸酚裂解酶突变体及其应用
EP4069859A1 (en) * 2019-12-03 2022-10-12 Crobio Ltd Genetically engineered microorganisms
WO2021221690A1 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
BR112022013753A2 (pt) * 2020-01-13 2023-03-14 Pivot Bio Inc Composição sintética de micróbios funcionalmente otimizados, e, método
WO2021173953A1 (en) * 2020-02-26 2021-09-02 Pebble Labs, Inc. Novel plant endophytic bacteria and methods to control plant pathogens and pests
US12281980B2 (en) 2020-05-01 2025-04-22 Pivot Bio, Inc. Measurement of nitrogen fixation and incorporation
CA3172322A1 (en) 2020-05-01 2021-11-04 Karsten TEMME Modified bacterial strains for improved fixation of nitrogen
JP7670304B2 (ja) * 2020-07-23 2025-04-30 株式会社アクトリー 新規菌株及び該菌株を含む植物生育促進剤
CA3195748A1 (en) * 2020-10-21 2022-04-28 Andes Ag, Inc. Compositions and methods for producing bicarbonate and minerals
WO2022155445A1 (en) * 2021-01-15 2022-07-21 Zymergen Inc. Non-naturally occurring host cells for enhanced plant growth
CN112608873A (zh) * 2021-01-19 2021-04-06 中国科学院兰州化学物理研究所 一种具有促生活性的类芽孢杆菌属细菌及其制备和应用
US20240294953A1 (en) 2021-07-02 2024-09-05 Pivot Bio, Inc. Genetically-engineered bacterial strains for improved fixation of nitrogen
CN113817732B (zh) * 2021-08-18 2023-12-01 中国农业科学院生物技术研究所 具有固氮基因沉默功能的人工非编码rna及其应用
CN115637241B (zh) * 2022-10-11 2023-09-12 四川农业大学 一株大豆根腐病防病促生细菌RH_Pc03及其应用
CR20250228A (es) * 2022-12-05 2025-07-11 Bioconsortia Inc Microbios de paenibacillus fijadores de nitrógeno
CN116333750A (zh) * 2022-12-30 2023-06-27 江苏大学 一种用于盐碱地的固氮藻絮团活性磷生物土壤改良剂及其制备方法
WO2024224588A1 (ja) * 2023-04-28 2024-10-31 エム・シー通商株式会社 無機培養土
WO2025024750A2 (en) * 2023-07-26 2025-01-30 Pivot Bio, Inc. Assessing nitrogenase proteins in rhizosphere to infer microbial functioning
CN118360182B (zh) * 2024-01-22 2024-10-22 中国农业大学 一株促进am真菌和作物生长的菌根帮助细菌及其应用

Family Cites Families (243)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1520545A (en) 1924-09-04 1924-12-23 Charles K Morganroth Transmission gearing
US4832728A (en) 1981-09-25 1989-05-23 Melamine Chemicals, Inc. Fertilizer compositions, processes of making them, and pocesses of using them
US4782022A (en) 1984-06-04 1988-11-01 Lubrizol Genetics, Inc. Nitrogen fixation regulator genes
US6548289B1 (en) 1984-12-28 2003-04-15 Land O'lakes, Inc. Biological nitrogen fixation
DK609885D0 (da) 1985-12-30 1985-12-30 Sven Erik Nielsen Fremgangsmaade til modifikation af organismer
US5229291A (en) 1985-12-30 1993-07-20 Novo Industri A/S Rhizobia transformants which symbiotically fixes nitrogen in non-legumes, a material for treating seeds of a non-legume plant, non-legume seeds, a non-legume plant and a method for producing rhizobia transconjungants
CA1335366C (en) 1986-08-19 1995-04-25 Joseph Kloepper Plant growth promoting rhizobacteria for agronomic nonroot crops
EP0292984A2 (en) 1987-05-29 1988-11-30 The General Hospital Corporation Cloned rhizobium meliloti ntrA (rpoN) gene
JPH01225483A (ja) 1988-03-04 1989-09-08 Takeshi Uozumi 組換え体プラスミド
EP0339830A3 (en) 1988-04-14 1990-01-17 Biotechnica International, Inc. Improved biological nitrogen fixation
DK0413019T3 (da) 1989-02-24 2001-11-12 Monsanto Technology Llc Syntetiske plantegener og fremgangsmåde til fremstilling af disse
US5188960A (en) 1989-06-27 1993-02-23 Mycogen Corporation Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins
US5116506A (en) 1989-06-30 1992-05-26 Oregon State University Support aerated biofilm reactor
US5071743A (en) 1989-10-27 1991-12-10 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Process for conducting site-directed mutagenesis
US5610044A (en) 1990-10-01 1997-03-11 Lam; Stephen T. Microorganisms with mannopine catabolizing ability
US5427785A (en) 1990-11-21 1995-06-27 Research Seeds, Inc. Rhizosheric bacteria
GB2259302A (en) 1991-09-09 1993-03-10 Anil Kumar Bali Mutant nitrogen fixing bacterium
CA2051071A1 (en) 1991-09-10 1993-03-11 Anil K. Bali Ammonia production
CA2126438C (en) 1991-12-24 2003-12-02 Jac A. Nickoloff Site-directed mutagenesis of dna
HU9201218D0 (en) 1992-04-10 1992-06-29 Eotvos Lorand Tudomanyegyetem Process for the entering of non-auxotrophic bacteries -belonging to the azotobacteraceae family - into the intercellular spaces of plant tissues, for their cultivation in vitro, with the aim to create new nitrogenfixing plant-bacterium symbiosis
US5877012A (en) 1993-03-25 1999-03-02 Novartis Finance Corporation Class of proteins for the control of plant pests
FR2718750B1 (fr) 1994-04-19 1996-06-14 Pasteur Institut Protéines recombinantes de l'hémagglutinine filamenteuse de Bordetella, notamment, B. Pertussis, production et application à la production de protéines étrangères ou de principes actifs vaccinants.
WO1996030509A1 (fr) 1995-03-30 1996-10-03 Takara Shuzo Co., Ltd. Promoteur vegetal et expression genetique l'utilisant
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US5789166A (en) 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
US6083499A (en) 1996-04-19 2000-07-04 Mycogen Corporation Pesticidal toxins
US5916029A (en) 1996-06-26 1999-06-29 Liphatech, Inc. Process for producing seeds coated with a microbial composition
US5780270A (en) 1996-07-17 1998-07-14 Promega Corporation Site-specific mutagenesis and mutant selection utilizing antibiotic-resistant markers encoding gene products having altered substrate specificity
CA2264482A1 (en) 1996-09-06 1998-03-12 The Trustees Of The University Of Pennsylvania An inducible method for production of recombinant adeno-associated viruses utilizing t7 polymerase
US6114148C1 (en) 1996-09-20 2012-05-01 Gen Hospital Corp High level expression of proteins
US6063756A (en) 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
US6713063B1 (en) 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
US5942664A (en) 1996-11-27 1999-08-24 Ecogen, Inc. Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants
HUP9701446A1 (hu) 1997-08-27 1999-05-28 Phylaxia-Pharma Gyógyszer-, Oltóanyag és Agrobiológiai Készítményeket Gyártó és Forgalmazó Rt. Eljárás a talaj mikroorganizmus populációjának előnyös kialakítására
US6218188B1 (en) 1997-11-12 2001-04-17 Mycogen Corporation Plant-optimized genes encoding pesticidal toxins
US6033861A (en) 1997-11-19 2000-03-07 Incyte Genetics, Inc. Methods for obtaining nucleic acid containing a mutation
AU5209399A (en) 1998-07-10 2000-02-01 Cornell Research Foundation Inc. Recombinant constructs and systems for secretion of proteins via type iii secretion systems
CN1324407A (zh) 1998-10-23 2001-11-28 麦考根公司 编码大约15kDa和大约45kDa的杀虫蛋白质的植物最优化的多核苷酸
US6489542B1 (en) 1998-11-04 2002-12-03 Monsanto Technology Llc Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids
PL350318A1 (en) 1999-03-23 2002-12-02 Merck Patent Gmbh Protein isolation and analysis
DK1947172T3 (da) 1999-07-27 2013-03-25 Food Industry Res & Dev Inst Konstruktion af metabolisk styring
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
JP3859947B2 (ja) 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
US20020061579A1 (en) 2000-08-09 2002-05-23 Farrand Stephen K. Counter selection strategy for Gram-negative bacteria
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
CN1289852A (zh) 2000-09-26 2001-04-04 国家人类基因组南方研究中心 一种人固氮基因同源蛋白及其编码序列
CN1129665C (zh) 2000-11-29 2003-12-03 中国科学院植物研究所 一种耐铵工程菌和含有这种工程菌的生物肥料
CN1132930C (zh) 2000-11-29 2003-12-31 中国科学院植物研究所 一种固氮菌和含有该固氮菌的生物肥料
AR035799A1 (es) 2001-03-30 2004-07-14 Syngenta Participations Ag Toxinas insecticidas aisladas de bacillus thuringiensis y sus usos.
JP2003033174A (ja) 2001-07-10 2003-02-04 Japan Science & Technology Corp 窒素固定能を増強した根粒菌
GB0121126D0 (en) 2001-08-31 2001-10-24 Univ Nottingham Systemic non-nodular endosymbiotic nitrogen fixation in plants
RU2230114C2 (ru) 2001-11-30 2004-06-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Мутантная глутаминсинтетаза, фрагмент днк, штамм escherichia coli - продуцент l-глутамина и способ получения l-аминокислот
US7084331B2 (en) 2002-01-15 2006-08-01 Society for Techno-Innovation of Agriculture Forestry and Fisheries Rice containing endophytic bacteria and method of producing it
BR0312064A (pt) 2002-06-26 2005-08-09 Du Pont Genes que codificam proteìnas com atividade pesticida
US7462760B2 (en) 2002-06-26 2008-12-09 Pioneer Hi-Bred International, Inc. Genes encoding plant protease-resistant pesticidal proteins and method of their use
CN100494185C (zh) 2002-08-28 2009-06-03 旭化成制药株式会社 新型季铵化合物
US20060112447A1 (en) 2002-08-29 2006-05-25 Bogdanova Natalia N Nucleotide sequences encoding cry1bb proteins for enhanced expression in plants
US20050266541A1 (en) 2002-11-04 2005-12-01 Harrison F. Dillon Methods and compositions for evolving microbial hydrogen production
CN1500801A (zh) 2002-11-18 2004-06-02 中国农业科学院原子能利用研究所 可提高联合固氮菌固氮水平的基因及其应用
AR043328A1 (es) 2003-01-21 2005-07-27 Dow Agrosciences Llc Metodo para la inhibicion de insectos
US20040210965A1 (en) 2003-02-20 2004-10-21 Athenix Corporation AXMI-007, a delta-endotoxin gene and methods for its use
US7355099B2 (en) 2003-02-20 2008-04-08 Athenix Corporation AXMI-004, a delta-endotoxin gene and methods for its use
US20040197917A1 (en) 2003-02-20 2004-10-07 Athenix Corporation AXMI-014, delta-endotoxin gene and methods for its use
US7351881B2 (en) 2003-02-20 2008-04-01 Athenix Corporation AXMI-008, a delta-endotoxin gene and methods for its use
US20040216186A1 (en) 2003-02-20 2004-10-28 Athenix Corporation AXMI-006, a delta-endotoxin gene and methods for its use
NZ578677A (en) 2003-02-20 2010-11-26 Athenix Corp AXMI-009, a delta-endotoxin gene and methods for its use
US20040210964A1 (en) 2003-02-20 2004-10-21 Athenix Corporation AXMI-009, a delta-endotoxin gene and methods for its use
CN1254533C (zh) 2003-06-02 2006-05-03 中国农业大学 含多拷贝nifA基因的巴西固氮螺菌DraT-工程菌株
HU0301909D0 (en) 2003-06-23 2003-08-28 Someus Edward Process for solid fermentation of microorganisms bound to bone black carrier amid for production, storage and uses of granular compositions
US8796026B2 (en) 2003-07-07 2014-08-05 Monsanto Technology Llc Insecticidal proteins secreted from Bacillus thuringiensis and uses therefor
US7253343B2 (en) 2003-08-28 2007-08-07 Athenix Corporation AXMI-003, a delta-endotoxin gene and methods for its use
US7205450B2 (en) 2003-10-08 2007-04-17 The Regents Of The University Of California DMI1 gene encodes a protein that is required for the early steps of bacterial and fungal symbioses
US20050183161A1 (en) 2003-10-14 2005-08-18 Athenix Corporation AXMI-010, a delta-endotoxin gene and methods for its use
BRPI0507896A (pt) 2004-02-20 2007-07-24 Pionner Hi Bred International lipases e métodos de utilização das mesmas na criação ou no aumento de resistência a insetos em plantas
BRPI0500788A (pt) 2004-03-05 2006-04-18 Agrigenetics Inc combinações de cry1ab e cry1fa como uma ferramenta de controle da resistência a insetos
WO2006005004A2 (en) 2004-06-30 2006-01-12 University Of Florida Research Foundation Inc. Materials and methods for enhancing nitrogen fixation in plants
EP1771389A1 (en) 2004-07-07 2007-04-11 National University of Ireland, Galway A biofilm reactor
AU2005101078A4 (en) 2004-07-12 2009-04-30 Zebra Holdings Pty Ltd Method and system for promoting microbial nitrogen fixation activity
EP1779155A2 (en) 2004-08-10 2007-05-02 Cardinal CG Company Lcd mirror system and method
CN1746304A (zh) 2004-09-10 2006-03-15 中国农业科学院生物技术研究所 固氮负调节基因突变的泌铵工程菌的构建及应用
US20060096918A1 (en) 2004-11-09 2006-05-11 Semmens Michael J Biofilm wastewater treatment devices
US7485451B2 (en) 2004-11-18 2009-02-03 Regents Of The University Of California Storage stable compositions of biological materials
WO2006083891A2 (en) 2005-01-31 2006-08-10 Athenix Corporation Axmi-018, axmi-020, and axmi-021, a family of delta-endotoxin genes and methods for their use
JP4677568B2 (ja) 2005-03-14 2011-04-27 国立大学法人 鹿児島大学 窒素固定活性の高い根粒を着生する植物の作出法
US7601498B2 (en) 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
NZ562795A (en) 2005-04-01 2009-07-31 Athenix Corp AXMI-036, a delta-endotoxin gene and methods for its use
US7622572B2 (en) 2005-05-02 2009-11-24 Athenix Corporation AXMI-028 and AXMI-029, a family of novel delta-endotoxin genes and methods for their use
AU2006284856B2 (en) 2005-08-31 2011-06-02 Monsanto Technology Llc Insecticidal compositions and methods for making insect-resistant transgenic plants
EP2314710B1 (en) 2006-01-04 2016-03-30 Metabolic Explorer Method for the production of methionine by culturing a microorganism modified to enhance production of cysteine
US20070249018A1 (en) 2006-02-23 2007-10-25 Goutham Vemuri Reduced overflow metabolism and methods of use
WO2007107000A1 (en) 2006-03-22 2007-09-27 Adjuvants Plus Inc. The production and use of endophytes as novel inoculants for promoting enhanced plant vigor, health, growth, yield reducing environmental stress and for reducing dependency on chemical pesticides for pest control
US7329736B2 (en) 2006-04-14 2008-02-12 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis cry gene and protein
US7449552B2 (en) 2006-04-14 2008-11-11 Pioneer Hi-Bred International, Inc. Bacillus thuringiensis cry gene and protein
EP3196311B1 (en) 2006-05-16 2019-12-04 Monsanto Technology LLC Use of non-agrobacterium bacterial species for plant transformation
US7923602B2 (en) 2006-06-14 2011-04-12 Athenix Corp. AXMI-031, AXMI-039, AXMI-040 and AXMI-049, a family of novel delta endotoxin genes and methods for their use
EP2455392A3 (en) 2006-06-15 2012-08-22 Athenix Corporation A family of pesticidal proteins and methods for their use
WO2008000809A1 (en) 2006-06-30 2008-01-03 Biogasol Ipr Aps Production of fermentation products in biofilm reactors using microorganisms immobilised on sterilised granular sludge
US8268584B1 (en) 2006-12-01 2012-09-18 University Of Washington Hydrogen production from microbial strains
CN101600801B (zh) 2006-12-08 2013-01-02 先锋高级育种国际公司 新苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物
US8076142B2 (en) 2006-12-21 2011-12-13 Basf Plant Sciences Gmbh Rooted plant assay system
FR2910230B3 (fr) 2006-12-22 2009-01-23 Pierre Philippe Claude Methodes de biofertilisations pour ameliorer la stabilite des rendements de grandes cultures agronomiques.
BRPI0809352B1 (pt) 2007-03-28 2017-03-28 Syngenta Participations Ag proteína inseticida híbrida projetada, molécula de ácido nucleico codificadora da dita proteína,cassete de expressão, vetor recombinante, célula hospedeira transgênica, composição inseticida, métodos de produção da dita proteína e de uma planta transgênica resistente a insetos e método de controle de insetos".
US20100267147A1 (en) 2007-04-25 2010-10-21 GM Biosciences, Inc. Site-directed mutagenesis in circular methylated dna
US8609936B2 (en) 2007-04-27 2013-12-17 Monsanto Technology Llc Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis
EP2186890A1 (en) 2007-07-31 2010-05-19 Nihon University Method for production of biofilm
EP2020437A1 (en) 2007-08-03 2009-02-04 Commissariat A L'energie Atomique (Nife)-hydrogenases having an improved resistance to dioxygen, process for obtaining them and their applications
CN101878222B (zh) 2007-10-16 2014-08-13 阿森尼克斯公司 AXMI-066:δ-内毒素蛋白及其使用方法
WO2009060012A2 (en) 2007-11-06 2009-05-14 Basf Se Plant health compositions comprising a beneficial microorganism and a pesticide
US20090162477A1 (en) 2007-12-21 2009-06-25 Daniel Nadel High yield maize derivatives
AU2009205716A1 (en) 2008-01-15 2015-04-16 Michigan State University Polymicrobial formulations for enhancing plant productivity
CN101663389B (zh) 2008-03-24 2011-12-28 清华大学 敲除酰胺酶基因的产腈水合酶工程菌及其构建方法和应用
JP2009232721A (ja) 2008-03-26 2009-10-15 Univ Of Miyazaki エンテロバクター属細菌を用いた植物栽培方法
US8401798B2 (en) 2008-06-06 2013-03-19 Dna Twopointo, Inc. Systems and methods for constructing frequency lookup tables for expression systems
UA121093C2 (uk) 2008-06-25 2020-04-10 Атенікс Корпорейшн Конструкція, яка містить токсиген, що кодує пестицидний білок, і спосіб її застосування для знищення напівтвердокрилих шкідників
AU2009266906B2 (en) 2008-07-02 2014-08-14 BASF Agricultural Solutions Seed US LLC AXMI-IL 5, AXMI-113, AXMI-005, AXMI-163 and AXMI-184 : VIP3A insecticidal proteins from Bacillus thuringiensis and methods for their use
CN101328477A (zh) 2008-07-23 2008-12-24 中国人民解放军军事医学科学院生物工程研究所 一种双向筛选系统及其应用
ES2534581T3 (es) 2008-12-23 2015-04-24 Athenix Corporation Gen AXMI-150 de la delta-endotoxina y métodos para su uso
US8709781B2 (en) 2009-01-09 2014-04-29 Syracuse University System and method for the heterologous expression of polyketide synthase gene clusters
CN102361983B (zh) 2009-01-23 2014-11-19 先锋国际良种公司 具有鳞翅目活性的新苏云金芽孢杆菌基因
EA201171006A1 (ru) 2009-02-05 2012-03-30 Атеникс Корпорейшн Вариантные гены axmi-r1 дельта-эндотоксина и способы их применения
WO2010090330A1 (ja) 2009-02-09 2010-08-12 協和発酵バイオ株式会社 L-アミノ酸の製造法
BRPI1009420A2 (pt) 2009-03-11 2016-03-01 Athenix Corp axmi-001, axmi-002, axmi-030, e axmi-045; genes de toxina e métodos para seu uso
WO2010105226A2 (en) 2009-03-13 2010-09-16 University Of Washington Endophytic yeast strains, methods for ethanol and xylitol production, methods for biological nitrogen fixation, and a genetic source for improvement of industrial strains
WO2010109436A1 (en) 2009-03-25 2010-09-30 Carepro Bioscience (P) Ltd Microbial formulation for widespread uesd in agricultural practices
US8481026B1 (en) 2009-04-17 2013-07-09 Peter J. Woodruff Bacteria with increased trehalose production and method for using the same in bioremediation
DK2419441T3 (en) 2009-04-17 2015-04-27 Dow Agrosciences Llc Insecticidal dig-3-cry toxins
US8334366B1 (en) 2009-04-29 2012-12-18 The United States Of America, As Represented By The Secretary Of Agriculture Mutant lycotoxin-1 peptide sequences for insecticidal and cell membrane altering properties
AU2010260305A1 (en) 2009-06-16 2011-12-15 Dow Agrosciences Llc DIG-11 insecticidal Cry toxins
MX346215B (es) 2009-07-02 2017-03-10 Athenix Corp Gen plaguicida axmi-205 y metodos para su uso.
US8772576B2 (en) 2009-09-03 2014-07-08 L. David Kuykendall Herbicide-resistant inoculant strains
BR112012005407A2 (pt) 2009-09-11 2019-09-24 Valent Bioscences Corp cepa bacteriana, composição pesticida, e, método para controlar pragas
CN102041241A (zh) 2009-10-20 2011-05-04 中国农业科学院生物技术研究所 高效泌铵的联合固氮菌株
RU2593961C2 (ru) 2009-12-16 2016-08-10 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи КОМБИНИРОВАННОЕ ПРИМЕНЕНИЕ БЕЛКОВ CRY1Ca И CRY1Fa ДЛЯ БОРЬБЫ С РЕЗИСТЕНТНОСТЬЮ У НАСЕКОМЫХ
BR112012014727B1 (pt) 2009-12-16 2019-09-03 Dow Agrosciences Llc método de controle de um inseto lagarta-do-cartucho
NZ601106A (en) 2009-12-16 2014-10-31 Dow Agrosciences Llc Use of cry1ab in combination with cry1be for management of resistant insects
US20120331589A1 (en) 2009-12-16 2012-12-27 Dow Agrosciences Llc COMBINED USE OF CRY1Da AND CRY1Fa PROTEINS FOR INSECT RESISTANCE MANAGEMENT
CN102753012B (zh) 2009-12-16 2016-03-09 陶氏益农公司 用于控制秋粘虫和欧洲玉米螟的杀虫蛋白组合和用于昆虫抗性管理的方法
BR112012014746B1 (pt) 2009-12-16 2024-03-05 Dow Agrosciences Llc Método para controlar o desenvolvimento da resistência de um inseto à uma proteína inseticida derivada de um bacillus thuringiensis, bem como composição e método para o controle de pragas de lepidópteros
JP5908408B2 (ja) 2009-12-16 2016-04-26 ダウ アグロサイエンシィズ エルエルシー 抵抗性昆虫対応のためのVip3AbとCry1Faとの併用
WO2011099024A1 (en) 2010-02-09 2011-08-18 Patel, Babubhai C. Preparation of novel bacterial based product for providing nuitrition essential for promoting plant growth
WO2011099019A1 (en) 2010-02-09 2011-08-18 Patel, Babubhai, C. Composition and method of preparation of bacterial based product that fix atmospheric nitrogen from air and makes available to plant
CN102892886A (zh) 2010-02-18 2013-01-23 阿森尼克斯公司 AXMI218、AXMI219、AXMI220、AXMI226、AXMI227、AXMI228、AXMI229、AXMI230、和AXMI231δ-内毒素基因以及它们的使用方法
BR122019005253A8 (pt) 2010-02-18 2022-07-05 Athenix Corp Molécula de ácido nucleico recombinante, vetor, célula hospedeira, polipeptídeo recombinante com atividade pesticida, composição, bem como métodos para o controle de uma população de pragas para matar uma praga, para a produção de um polipeptídeo com atividade pesticida, para a proteção de uma planta de uma praga, e para aumentar o rendimento em uma planta
UA112516C2 (uk) 2010-04-23 2016-09-26 Дау Аґросаєнсиз Елелсі ТРАНСГЕННА РОСЛИНА, ЯКА ПРОДУКУЄ БІЛОК Сry34Ab1, БІЛОК Сry35Ab1 І ІНСЕКТИЦИДНИЙ БІЛОК Сry3Ba1, ДЛЯ ЗАПОБІГАННЯ РОЗВИТКУ СТІЙКОСТІ В КУКУРУДЗЯНИХ КОРЕНЕВИХ ЖУКІВ (Diabrotica spp.)
CN101880676A (zh) 2010-05-20 2010-11-10 黑龙江大学 导入nifA基因的大豆根瘤菌基因工程菌株HD-SFH-01的构建方法
US9228240B2 (en) 2010-06-03 2016-01-05 California Institute Of Technology Methods for detecting and quantifying viable bacterial endo-spores
WO2011154960A1 (en) 2010-06-09 2011-12-15 Patel, Babubhai C. Advance material and method of preparation of bacterial formulation using nitrogen fixing bacteria that fix atmoshpheric nitrogen and make available to crop plant
UA111592C2 (uk) 2010-07-07 2016-05-25 Сінгента Партісіпейшнс Аг Спосіб контролю над твердокрилими комахами-шкідниками
CN101899430A (zh) 2010-07-12 2010-12-01 甘肃农业大学 一种高效固氮微生物诱变育种方法
CA2807375A1 (en) 2010-08-19 2012-02-23 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with lepidopteran activity
CA2807171A1 (en) 2010-08-19 2012-02-23 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with lepidopteran activity
WO2012088369A2 (en) 2010-12-23 2012-06-28 The Ohio State University Fertilizer composition and method
CN103763916B (zh) 2011-02-11 2017-07-28 孟山都技术公司 杀虫核酸和蛋白质及其用途
CN102690808B (zh) 2011-03-23 2017-04-19 北京大学 为真核表达的目的构建原核基因表达岛
BR112013024609B1 (pt) 2011-03-31 2018-11-27 Novozymes Biologicals, Inc. composição, e, método para intensificar o crescimento da planta
AU2012240004B2 (en) 2011-04-07 2017-03-02 Monsanto Technology Llc Insect inhibitory toxin family active against Hemipteran and/or Lepidopteran insects
US8513494B2 (en) 2011-04-08 2013-08-20 Chunren Wu Plants and seeds of spring canola variety SCV695971
US9062323B2 (en) 2011-04-11 2015-06-23 The Regents Of The University Of California Identification and use of KRP mutants in wheat
US9392790B2 (en) 2011-05-06 2016-07-19 The Research Foundation For The State University Of New York Molecular roadblocks for RpoN binding sites
WO2012162533A2 (en) 2011-05-25 2012-11-29 Sam Houston State University Bioremediation reactor systems
US20130005590A1 (en) 2011-06-06 2013-01-03 The Regents Of The University Of California Synthetic biology tools
PL2721153T3 (pl) * 2011-06-16 2020-03-31 The Regents Of The University Of California Syntetyczne klastry genów
WO2012174646A1 (en) 2011-06-21 2012-12-27 University Of Guelph Screening for nitrogen fixation
RU2014107672A (ru) 2011-07-28 2015-09-10 Атеникс Корп. Ген токсина axmi270 и способы его применения
EA035432B1 (ru) 2011-07-29 2020-06-15 Басф Агрикалчерал Солюшнс Сид Юс Ллк Ген пестицида axmi279 и способы его применения
CN102417882A (zh) 2011-10-25 2012-04-18 中国农业科学院生物技术研究所 固氮斯氏假单胞菌A1501 rpoN基因的表达方法
HUE046334T2 (hu) 2011-11-04 2020-02-28 Terragen Holdings Ltd Mikrobális oltóanyagok és azokat tartalmazó trágyakészítmények
AR083981A1 (es) 2011-11-24 2013-04-10 Consejo Nac Invest Cient Tec Cepa de bacteria recombinante fijadora de nitrogeno, inoculo que la contiene y metodos de aplicacion
WO2013132518A1 (en) 2012-03-03 2013-09-12 Department Of Biotechnology Ministry Of Science & Technology Recombinant nitrogen fixing microorganism and uses thereof
US9580363B2 (en) 2012-03-21 2017-02-28 Temasek Life Sciences Laboratory Limited Nitrogen-fixing bacterial inoculant for improvement of crop productivity and reduction of nitrous oxide emission
US9398770B2 (en) 2012-05-30 2016-07-26 Bayer Cropscience Ag Compositions comprising a biological control agent and an insecticide
WO2013178661A1 (en) 2012-05-30 2013-12-05 Bayer Cropscience Ag Compositiions comprising a biological control agent and an insecticide
WO2014042517A2 (en) 2012-09-14 2014-03-20 Universiti Putra Malaysia Biofertilizer
EP3502235B1 (en) 2012-09-19 2025-08-20 BioConsortia, Inc. Methods of screening for microorganisms that impart beneficial properties to plants
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
JP6024963B2 (ja) 2012-11-13 2016-11-16 国立大学法人東京農工大学 新規バチルス属窒素固定細菌、植物生育促進剤、及び植物の栽培方法
EP4286403A3 (en) 2012-12-12 2024-02-14 The Broad Institute Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
RU2723946C2 (ru) 2013-02-05 2020-06-18 Юниверсити Оф Сэскэтчевэн Эндофитные микробные симбионты в пренатальном уходе за растениями
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US9937207B2 (en) 2013-03-21 2018-04-10 Sangamo Therapeutics, Inc. Targeted disruption of T cell receptor genes using talens
US10212943B2 (en) 2013-06-10 2019-02-26 The Regents Of The University Of California Plant growth-promoting microorganisms and methods of use thereof
CN103451130B (zh) 2013-07-25 2014-11-26 中国农业大学 一种固氮基因簇及其应用
JP6267073B2 (ja) 2013-07-26 2018-01-24 株式会社前川製作所 Escherichia属細菌の新規農業用途
JP6241916B2 (ja) 2013-08-18 2017-12-06 国立大学法人島根大学 サツマイモの栽培方法
GB2535911B (en) 2013-10-25 2020-10-21 Sound Agriculture Company Strigolactone formulations and uses thereof
CN115710559A (zh) 2013-12-04 2023-02-24 新叶共生有限公司 用于改良玉米产量的方法和组合物
JP6296776B2 (ja) 2013-12-16 2018-03-20 京都府 バイオ肥料の製造方法
CA2883596A1 (en) 2014-02-26 2015-08-26 Bioponix Technologies Inc. Continuous bioprocess for organic greenhouse agriculture
JP6489676B2 (ja) * 2014-03-17 2019-03-27 国立大学法人名古屋大学 生物窒素固定制御因子及びその利用
CA2945542C (en) 2014-04-17 2024-03-05 Glycovaxyn Ag Modified host cells and uses thereof
MA56478A (fr) 2014-05-23 2022-05-11 Bioconsortia Inc Procédé d'amélioration des plantes intégré pour appariements complémentaires de plantes et de consortiums microbiens
US11898141B2 (en) 2014-05-27 2024-02-13 The Broad Institute, Inc. High-throughput assembly of genetic elements
AU2015273608C1 (en) 2014-06-10 2019-08-15 Afyx Therapeutics A/S Compositions comprising electrohydrodynamically obtained fibres for administration of specific dosages of an active substance to skin or mucosa
GB201413335D0 (en) 2014-07-28 2014-09-10 Azotic Technologies Ltd Agricultural methods
GB201413333D0 (en) 2014-07-28 2014-09-10 Azotic Technologies Ltd Plant inoculation
US9800220B2 (en) 2014-09-24 2017-10-24 Intel Corporation Audio system with noise interference mitigation
WO2016100727A1 (en) 2014-12-18 2016-06-23 The Regents Of The University Of California Recombinantly engineered diazotrophs for whole cell hydrocarbon production and methods for making and using them
CA2972912A1 (en) 2015-01-15 2016-07-21 Steven GRUVER Insecticidal proteins and methods for their use
BR112017017003A2 (pt) 2015-02-09 2018-06-26 Bioconsortia Inc micróbios agricolamente benéficos, composições microbianas e consórcios
US9796957B2 (en) 2015-03-11 2017-10-24 Regents Of The University Of Minnesota Genetically modified diazotrophs and methods of using same
FR3033790B1 (fr) 2015-03-19 2018-05-04 Universite Claude Bernard Lyon I Utilisation de proanthocyanidines pour limiter la denitrification
US10913939B2 (en) 2015-04-01 2021-02-09 Monsanto Technology Llc Compositions and methods for expression of nitrogenase in plant cells
AU2016253008B2 (en) 2015-04-24 2020-10-22 Sound Agriculture Company Methods for hydraulic enhancement of crops
AU2016258912A1 (en) 2015-05-01 2017-11-30 Indigo Agriculture, Inc. Isolated complex endophyte compositions and methods for improved plant traits
NL2014777B1 (en) 2015-05-07 2017-01-26 Ibema Biezenmortel B V Nitrifying micro-organisms for fertilisation.
AU2016259871B2 (en) 2015-05-11 2020-04-16 Mybiotics Pharma Ltd Systems and methods for growing a biofilm of probiotic bacteria on solid particles for colonization of bacteria in the gut
CN108290799A (zh) 2015-06-05 2018-07-17 可持续有机解决方案私人有限公司 微生物接种剂、肥料组合物、生长基质和增强植物生长的方法
KR102197507B1 (ko) 2015-07-13 2020-12-31 피벗 바이오, 인크. 식물 형질 개선을 위한 방법 및 조성물
WO2017042833A1 (en) 2015-09-11 2017-03-16 Zydex Industries Pvt. Ltd. Bio-fertilizer composition
WO2017062412A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
US10131585B2 (en) 2015-10-19 2018-11-20 California Institute Of Technology Hopanoids producing bacteria and related biofertilizers, compositions, methods and systems
TR201513059A1 (tr) 2015-10-20 2019-01-21 Ibrahim Isildak Bi̇r bi̇yogübre formülasyonu
ES2830251T3 (es) 2015-11-19 2021-06-03 Univ Basel Administración de proteínas basada en bacterias
WO2017112827A1 (en) 2015-12-21 2017-06-29 Indigo Agriculture, Inc. Endophyte compositions and methods for improvement of plant traits in plants of agronomic importance
EP3231874A1 (en) 2016-04-14 2017-10-18 Curetis GmbH Using the full repertoire of genetic information from bacterial genomes and plasmids for improved genetic resistance tests
ITUA20163807A1 (it) 2016-05-25 2017-11-25 Univ Degli Studi Di Foggia Metodo per la produzione di biofilm microbici probiotici e relativi usi
CN106086042A (zh) 2016-06-30 2016-11-09 上海交通大学 固氮菌铵载体基因、突变体、固氮菌铵载体突变株及应用
WO2018081543A1 (en) 2016-10-28 2018-05-03 Bayer Cropscience Lp Mutants of bacillus and methods for their use
CN116904333A (zh) * 2017-01-12 2023-10-20 皮沃特生物公司 用于改良植物性状的方法及组合物
CN106658654A (zh) 2017-01-22 2017-05-10 北京佰才邦技术有限公司 软sim的控制方法及用户终端
KR20200044015A (ko) 2017-08-09 2020-04-28 피벗 바이오, 인크. 유전자조작 미생물을 개선하기 위한 방법 및 조성물
US10525318B2 (en) 2017-09-12 2020-01-07 Edmond J. Dougherty Timing display device
EP3701040A4 (en) 2017-10-25 2021-08-25 Pivot Bio, Inc. METHODS AND COMPOSITIONS FOR IMPROVING GENETICALLY MODIFIED MICROBES THAT BIND NITROGEN
AU2018354338B2 (en) 2017-10-25 2023-10-26 Pivot Bio, Inc. Gene targets for nitrogen fixation targeting for improving plant traits
EP3737740A1 (en) 2018-01-10 2020-11-18 Bayer CropScience LP Improved microbes and methods for producing the same
CA3104531A1 (en) 2018-06-27 2020-01-02 Pivot Bio, Inc. Guided microbial remodeling, a platform for the rational improvement of microbial species for agriculture
EP3814302A4 (en) 2018-06-27 2022-06-29 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
WO2020014498A1 (en) 2018-07-11 2020-01-16 Pivot Bio, Inc. Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes
WO2020023630A1 (en) 2018-07-25 2020-01-30 Convergent Genomics, Inc. Urinary microbiomic profiling
CN112867784A (zh) 2018-09-21 2021-05-28 皮沃特生物股份有限公司 用于改善磷酸盐增溶的方法和组合物
AR116964A1 (es) 2018-11-01 2021-06-30 Pivot Bio Inc Composiciones de biopelículas con estabilidad mejorada para fijación del nitrógeno en productos microbianos
US20220106238A1 (en) 2018-12-07 2022-04-07 Pivot Bio, Inc. Polymer compositions with improved stability for nitrogen fixing microbial products
CN113825393A (zh) 2018-12-21 2021-12-21 皮沃特生物股份有限公司 改善植物性状的方法、组合物和培养基
US12421519B2 (en) 2019-01-07 2025-09-23 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
JP2022519289A (ja) 2019-02-05 2022-03-22 ピボット バイオ, インコーポレイテッド 生物学的窒素固定による作物収穫高の一貫性の向上
US12281299B2 (en) 2019-03-19 2025-04-22 Massachusetts Institute Of Technology Control of nitrogen fixation in rhizobia that associate with cereals
KR20220023756A (ko) 2019-04-24 2022-03-02 피벗 바이오, 인크. 식물 특성 향상을 위한 질소 고정 표적화용 유전자 표적
PH12021552660A1 (en) 2019-04-25 2022-10-03 Pivot Bio Inc High-throughput methods for isolating and characterizing ammoniumexcreting mutant libraries generated by chemical mutagenesis
US20230019267A1 (en) 2019-12-04 2023-01-19 Pivot Bio, Inc. System to deliver a solution with a biological product in a planter assembly
BR112022013753A2 (pt) 2020-01-13 2023-03-14 Pivot Bio Inc Composição sintética de micróbios funcionalmente otimizados, e, método
US20230257317A1 (en) 2020-05-01 2023-08-17 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
BR112022022714A2 (pt) 2020-05-13 2023-03-28 Pivot Bio Inc Bactéria diazotrófica gram-positiva geneticamente modificada, composição microbiana, método para fornecer nitrogênio fixo a uma planta, gene glnr, proteína glnr, e, método para identificar reguladores de um operon nif

Also Published As

Publication number Publication date
AU2025205502A1 (en) 2025-08-07
US20200308594A1 (en) 2020-10-01
US11993778B2 (en) 2024-05-28
BR112020008035A2 (pt) 2020-10-27
JP7420712B2 (ja) 2024-01-23
JP2021500906A (ja) 2021-01-14
JP2023159378A (ja) 2023-10-31
KR20200088342A (ko) 2020-07-22
AU2018354221B2 (en) 2025-04-17
US20240327851A1 (en) 2024-10-03
CN111587287B (zh) 2025-02-18
CN120041362A (zh) 2025-05-27
RU2020116764A3 (es) 2022-04-28
AU2018354221A1 (en) 2020-05-14
CN111587287A (zh) 2020-08-25
EP3701040A4 (en) 2021-08-25
PH12020550484A1 (en) 2021-03-22
CA3079955A1 (en) 2019-05-02
WO2019084059A2 (en) 2019-05-02
RU2020116764A (ru) 2021-11-25
EP3701040A2 (en) 2020-09-02

Similar Documents

Publication Publication Date Title
MX2020004343A (es) Métodos y composiciones para mejorar microbios modificados que fijan nitrógeno.
UY37566A (es) Métodos y composiciones para mejorar características en plantas
MX2024010722A (es) Objetivos geneticos para el direccionamiento de fijacion de nitrogeno para mejorar los rasgos de las plantas.
MX2021000308A (es) Composiciones de fusosoma y usos de estas.
MX2020001599A (es) Métodos y composiciones para mejorar los microbios modificados por ingeniería.
BR112018004670A2 (pt) composição inoculante não aquosa, material de propagação em planta revestida, kit, planta, parte de planta, produto processado, cultura, e, método
MX393437B (es) Composiciones microbianas definidas.
BR112018013111A2 (pt) composição inoculante não aquosa, material de propagação de planta revestido, kit, planta, parte de planta, produto processado, cultura, e, método.
MX2017010294A (es) Microbios, composiciones de microbios, y consorcios beneficiosos para la agricultura.
PH12021550781A1 (en) Compositions and methods for controlling plant pests and improving plant health
PH12021550994A1 (en) Bacterial compositions and methods for controlling plant pests and improving plant health
AR111835A1 (es) Composiciones estables de inoculantes que comprenden aceites vegetales metilados
MX2017000890A (es) Composiciones que contienen lipo-quitooligosacarido y microorganismo para potenciar el crecimiento de las plantas.
UY37562A (es) Cepas bacterianas, composiciones que las comprenden, plantas y semillas tratadas con éstas y métodos relacionados
ZA202004679B (en) Regeneration of genetically modified plants
AR052605A1 (es) Semilla de maiz con contenido de lisina mejorado sinergicamente
PH12017501503B1 (en) Acid composition based on leonardite and amino acids
AR115081A1 (es) Aislados de microbacterias y sus usos
ECSP17040052A (es) Consorcios bacterianos sinergísticos para movilizar fósforo del suelo
MX393266B (es) Composiciónpara usarse en terapia de microbiota
MX2018006221A (es) Composicion y metodos para reducir la penalizacion de rendimiento de maiz a maiz.
MX389692B (es) Composiciones y métodos para promover el crecimiento del cabello con inhibidores de mpc.
CL2021001246A1 (es) Moduladores de expresión irf5.
MX2021010057A (es) Metodos y composiciones para espantar aves de plantas de cultivos.
AR111561A1 (es) Método para mejorar la seguridad de los cultivos