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MX2021012157A - Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos. - Google Patents

Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos.

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Publication number
MX2021012157A
MX2021012157A MX2021012157A MX2021012157A MX2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A MX 2021012157 A MX2021012157 A MX 2021012157A
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Mexico
Prior art keywords
bacillus
genome
methods
compositions
recombinant dna
Prior art date
Application number
MX2021012157A
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English (en)
Inventor
Wonchul Suh
Ryan L Frisch
Stacey Irene Robida Stubbs
Derek Joseph Zimmer
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2021012157A publication Critical patent/MX2021012157A/es

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    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
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Abstract

Se proporcionan métodos y composiciones para la integración de genes de interés en el genoma de una célula de Bacillus sp. sin la integración de un marcador seleccionable en los genoma. Los métodos emplean un sistema de ADN recombinante circular dual para la introducción de un sistema de endonucleasa ARN guía/Cas (también denominado endonucleasa guiada por ARN, RGEN), así como un ADN donante en una célula de Bacillus sp., y proporcionando un sistema altamente eficaz para insertar genes de interés en el genoma de la célula de Bacillus sp.
MX2021012157A 2019-04-05 2020-04-03 Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos. MX2021012157A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962829664P 2019-04-05 2019-04-05
PCT/US2020/026503 WO2020206197A1 (en) 2019-04-05 2020-04-03 Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof

Publications (1)

Publication Number Publication Date
MX2021012157A true MX2021012157A (es) 2022-01-06

Family

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MX2021012157A MX2021012157A (es) 2019-04-05 2020-04-03 Métodos para la integración de polinucleótidos en el genoma de bacillus usando constructos de adn recombinante circular dual y composiciones de los mismos.

Country Status (7)

Country Link
US (1) US20220162621A1 (es)
EP (1) EP3947656A1 (es)
JP (1) JP2022526414A (es)
KR (1) KR20210148270A (es)
CA (1) CA3136113A1 (es)
MX (1) MX2021012157A (es)
WO (1) WO2020206197A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2023015460A (es) 2021-06-24 2024-01-18 Basf Se Celula huesped de bacillus mejorada con proteina rema/remb alterada.
EP4359423A1 (en) 2021-06-24 2024-05-01 Basf Se Bacillus licheniformis host cell for production of a compound of interest with increased purity
WO2022269082A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus production host
US20250051822A1 (en) 2021-12-20 2025-02-13 Basf Se Method for improved production of intracellular proteins in bacillus
CN116042401B (zh) * 2023-02-20 2024-07-19 厦门大学 从环境样品中靶向分离纯化目标微生物的方法
WO2025031865A1 (en) 2023-08-08 2025-02-13 Basf Se Improved bacillus cell with inactivated metalloprotease

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JP2022526414A (ja) 2022-05-24
WO2020206197A1 (en) 2020-10-08
CA3136113A1 (en) 2020-10-08
EP3947656A1 (en) 2022-02-09
US20220162621A1 (en) 2022-05-26
KR20210148270A (ko) 2021-12-07

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