AR008364A1 - PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE - Google Patents
PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDUREInfo
- Publication number
- AR008364A1 AR008364A1 ARP990100680A AR008364A1 AR 008364 A1 AR008364 A1 AR 008364A1 AR P990100680 A ARP990100680 A AR P990100680A AR 008364 A1 AR008364 A1 AR 008364A1
- Authority
- AR
- Argentina
- Prior art keywords
- procedure
- recombinant human
- oversighters
- humanarecombinant
- erythropoyetin
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 7
- 101000987586 Homo sapiens Eosinophil peroxidase Proteins 0.000 title abstract 2
- 102000044890 human EPO Human genes 0.000 title abstract 2
- 101000920686 Homo sapiens Erythropoietin Proteins 0.000 title 1
- 238000012656 cationic ring opening polymerization Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 108010092408 Eosinophil Peroxidase Proteins 0.000 abstract 2
- 238000011026 diafiltration Methods 0.000 abstract 2
- 230000004071 biological effect Effects 0.000 abstract 1
- 238000005277 cation exchange chromatography Methods 0.000 abstract 1
- 238000002270 exclusion chromatography Methods 0.000 abstract 1
- 238000004128 high performance liquid chromatography Methods 0.000 abstract 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
Landscapes
- Peptides Or Proteins (AREA)
Abstract
Se refiere a un método para la obtencion de EPO humana recombinante de alto grado de pureza. El procedimiento reivindicado comprende, además,evitar exponer la proteína a condiciones extremas de temperatura y a solventes orgánicos que pudierana fectar su actividad biologica o resultar toxicos parasu uso en seres humanos. El procedimiento consiste en una precipitacion diferencial, una cromatografía de interaccion hidrofobica, concentracion ydiafiltracion, cromatografía sucesivaanion ica y cationica, nueva concentracion y diafiltracion y cromatografía de exclusion molecular. El procedimiento sedistingue por no utilizar pasos de cromatografía líquida de alta presion y por un alto porcentaje de recuperacion de EPO.Comprende t ambién a la EPOobtenida segun el procedimiento descripto.It refers to a method for obtaining high purity recombinant human EPO. The claimed procedure also includes avoiding exposing the protein to extreme temperature conditions and organic solvents that could affect its biological activity or be toxic for use in humans. The procedure consists of differential precipitation, hydrophobic interaction chromatography, concentration and diafiltration, successive ion and cation chromatography, fresh concentration and diafiltration and molecular exclusion chromatography. The procedure is distinguished by not using high pressure liquid chromatography steps and a high percentage of EPO recovery. It also includes the EPO obtained according to the described procedure.
Priority Applications (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP990100680 AR008364A1 (en) | 1999-02-23 | 1999-02-23 | PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE |
| BR9917606-8A BR9917606A (en) | 1998-11-06 | 1999-07-07 | Procedure for the purification of recombinant human erythropoietin from cell culture supernatants and recombinant human erythropoietin obtained with such procedure |
| CO99069965A CO5241324A1 (en) | 1998-11-06 | 1999-11-05 | PROCEDURE FOR PURIFICATION OF RECOMBINANT HUMAN ERYTHROPOYETIN FROM CELLULAR CROPS OVERRANKS. . |
| CA2704598A CA2704598A1 (en) | 1998-11-06 | 1999-11-08 | Methods of purifying recombinant human erythropoietin from cell culture supernatants |
| DE69939268T DE69939268D1 (en) | 1998-11-06 | 1999-11-08 | METHODS FOR CLEANING RECOMBINATIVE HUMAN ERYTHROPOIETIN FROM CELL CULTURAL RESEARCH |
| PCT/US1999/026241 WO2000027869A1 (en) | 1998-11-06 | 1999-11-08 | Methods of purifying recombinant human erythropoietin from cell culture supernatants |
| AT99958811T ATE403671T1 (en) | 1998-11-06 | 1999-11-08 | METHODS FOR THE PURIFICATION OF RECOMBINATE HUMAN ERYTHROPOIETIN FROM CELL CULTURE SUPERNATIVES |
| JP2000581046A JP4516692B2 (en) | 1998-11-06 | 1999-11-08 | Method for purifying recombinant human erythropoietin derived from cell culture supernatant |
| ES99958811T ES2312219T3 (en) | 1998-11-06 | 1999-11-08 | METHODS TO PURIFY HUMAN ERYTHROPOYETIN RECOMBINANT OF CELLULAR CROP OVERRANKS. |
| EP99958811A EP1127063B1 (en) | 1998-11-06 | 1999-11-08 | Methods of purifying recombinant human erythropoietin from cell culture supernatants |
| CA2350327A CA2350327C (en) | 1998-11-06 | 1999-11-08 | Methods of purifying recombinant human erythropoietin from cell culture supernatants |
| AU16100/00A AU1610000A (en) | 1998-11-06 | 1999-11-08 | Methods of purifying recombinant human erythropoietin from cell culture supernatants |
| US09/830,964 US7012130B1 (en) | 1998-11-06 | 1999-11-08 | Methods of purifying recombinant human erythropoietin from cell culture supernatants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ARP990100680 AR008364A1 (en) | 1999-02-23 | 1999-02-23 | PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR008364A1 true AR008364A1 (en) | 2000-01-19 |
Family
ID=45724369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990100680 AR008364A1 (en) | 1998-11-06 | 1999-02-23 | PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR008364A1 (en) |
-
1999
- 1999-02-23 AR ARP990100680 patent/AR008364A1/en not_active Application Discontinuation
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