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AR080434A1 - Metodo para purificar celulas derivadas de celulas madre pluripotenciales - Google Patents

Metodo para purificar celulas derivadas de celulas madre pluripotenciales

Info

Publication number
AR080434A1
AR080434A1 ARP110100624A ARP110100624A AR080434A1 AR 080434 A1 AR080434 A1 AR 080434A1 AR P110100624 A ARP110100624 A AR P110100624A AR P110100624 A ARP110100624 A AR P110100624A AR 080434 A1 AR080434 A1 AR 080434A1
Authority
AR
Argentina
Prior art keywords
cells
lineage
population
characteristic markers
express characteristic
Prior art date
Application number
ARP110100624A
Other languages
English (en)
Original Assignee
Centocor Ortho Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centocor Ortho Biotech Inc filed Critical Centocor Ortho Biotech Inc
Publication of AR080434A1 publication Critical patent/AR080434A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Métodos para diferenciar las células madre pluripotentes. En particular, la presente solicitud proporciona métodos de caracterizacion de células diferenciadas en células que expresan marcadores característicos del linaje pancreático endocrino mediante marcadores de superficie unicos. También proporciona métodos para enriquecer o clasificar las células que expresan marcadores característicos del linaje pancreático endocrino. Se proveen además métodos para reducir las células que puedan contaminar las poblaciones de células que expresan marcadores característicos del linaje pancreático endocrino, formadas mediante los métodos revelados; lo que reduce la incidencia en la formacion de tumores in vivo después de un trasplante. Reivindicacion 1: Un método para mejorar la generacion de células productoras de insulina a partir de una poblacion de células que expresan marcadores característicos del linaje endocrino pancreático, el método comprende las etapas de: a) cultivar una poblacion de células madre pluripotentes, b) diferenciar la poblacion de células madre pluripotentes en una poblacion de células que expresan marcadores característicos del linaje endodermo definitivo, c) diferenciar la poblacion de células que expresan marcadores característicos del linaje de endodermo definitivo en células que expresan marcadores característicos del linaje del tubo intestinal primario, d) diferenciar la poblacion de células que expresan marcadores característicos del linaje del tubo intestinal primario en una poblacion de células que expresan marcadores característicos del linaje de endodermo pancreático, e) diferenciar la poblacion de células que expresan marcadores característicos del linaje del endodermo pancreático en una poblacion de células que expresan marcadores característicos del linaje endocrino pancreático, y f) trasplantar al menos algunas de las células que expresan marcadores característicos del linaje del endodermo pancreático en un animal mamífero.
ARP110100624A 2010-03-01 2011-03-01 Metodo para purificar celulas derivadas de celulas madre pluripotenciales AR080434A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US30919310P 2010-03-01 2010-03-01

Publications (1)

Publication Number Publication Date
AR080434A1 true AR080434A1 (es) 2012-04-11

Family

ID=44505394

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110100624A AR080434A1 (es) 2010-03-01 2011-03-01 Metodo para purificar celulas derivadas de celulas madre pluripotenciales

Country Status (15)

Country Link
US (2) US9969981B2 (es)
EP (1) EP2542667A4 (es)
JP (2) JP6013196B2 (es)
KR (2) KR20130025375A (es)
CN (2) CN107189979B (es)
AR (1) AR080434A1 (es)
AU (1) AU2011223900A1 (es)
BR (1) BR112012022145A2 (es)
CA (1) CA2791476C (es)
MX (1) MX358451B (es)
PH (2) PH12012501686A1 (es)
RU (2) RU2702198C2 (es)
SG (2) SG183535A1 (es)
WO (1) WO2011109279A2 (es)
ZA (1) ZA201708538B (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107189979B (zh) * 2010-03-01 2021-05-04 詹森生物科技公司 纯化衍生自多能干细胞的细胞的方法
WO2012175633A1 (en) 2011-06-21 2012-12-27 Novo Nordisk A/S Efficient induction of definitive endoderm from pluripotent stem cells
HK1203552A1 (en) * 2011-12-22 2015-10-30 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
CN104583392A (zh) * 2012-08-22 2015-04-29 耶达研究与发展有限公司 分离不同胰腺细胞类型的方法
WO2014201167A1 (en) * 2013-06-11 2014-12-18 President And Fellows Of Harvard College SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
WO2016100909A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED β CELLS AND USES THEREOF
US10253298B2 (en) 2014-12-18 2019-04-09 President And Fellows Of Harvard College Methods for generating stem cell-derived beta cells and methods of use thereof
USD772896S1 (en) 2015-02-06 2016-11-29 Samsung Electronics Co., Ltd. Display screen or portion thereof with icon
KR101643555B1 (ko) * 2015-03-12 2016-07-28 한국과학기술원 Stra6를 이용한 기능적으로 성숙된 인간 줄기세포 유래 간세포의 선별 방법
US10590392B2 (en) 2017-01-09 2020-03-17 Trustees Of Boston University Generation of airway epithelial organoids from human pluripotent stem cells
CA3049847A1 (en) * 2017-02-24 2018-08-30 Trustees Of Boston University Isolation of human lung progenitors derived from pluripotent stem cells
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
KR102738995B1 (ko) * 2017-09-11 2024-12-06 노보 노르디스크 에이/에스 시험관 내 줄기 세포로부터 유래된 nkx6.1 및 c-펩티드 공발현 세포의 농축
IL305391B2 (en) 2017-11-15 2024-09-01 Vertex Pharma Preparations for the production of islet cells and methods of use
CA3108275A1 (en) 2018-08-10 2020-02-13 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
WO2020243663A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CN114206407A (zh) 2019-05-31 2022-03-18 W.L.戈尔及同仁股份有限公司 生物相容性膜复合材料
JP7328362B2 (ja) 2019-05-31 2023-08-16 ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド 制御された酸素拡散距離を伴う細胞カプセル化デバイス
JP2022534545A (ja) 2019-05-31 2022-08-01 ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド 生体適合性メンブレン複合体
EP4027190A4 (en) 2019-09-04 2022-08-10 BOE Technology Group Co., Ltd. Line recognition device and display device
CN117355733A (zh) * 2021-03-15 2024-01-05 国立大学法人东北大学 载荷传感器以及载荷检测装置
IL311468A (en) * 2021-09-22 2024-05-01 Imagine Pharma Llc Compositions and methods for the proliferation of insulin- and glucagon-secreting cells from type 1 diabetic pancreatic tissue and their therapeutic uses

Family Cites Families (264)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3209652A (en) * 1961-03-30 1965-10-05 Burgsmueller Karl Thread whirling method
AT326803B (de) * 1968-08-26 1975-12-29 Binder Fa G Maschenware sowie verfahren zur herstellung derselben
US3935067A (en) * 1974-11-22 1976-01-27 Wyo-Ben Products, Inc. Inorganic support for culture media
CA1201400A (en) 1982-04-16 1986-03-04 Joel L. Williams Chemically specific surfaces for influencing cell activity during culture
US4499802A (en) * 1982-09-29 1985-02-19 Container Graphics Corporation Rotary cutting die with scrap ejection
US4537773A (en) * 1983-12-05 1985-08-27 E. I. Du Pont De Nemours And Company α-Aminoboronic acid derivatives
US4557264A (en) * 1984-04-09 1985-12-10 Ethicon Inc. Surgical filament from polypropylene blended with polyethylene
US5215893A (en) * 1985-10-03 1993-06-01 Genentech, Inc. Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US5089396A (en) * 1985-10-03 1992-02-18 Genentech, Inc. Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US4737578A (en) * 1986-02-10 1988-04-12 The Salk Institute For Biological Studies Human inhibin
US5863531A (en) * 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
CA1340581C (en) * 1986-11-20 1999-06-08 Joseph P. Vacanti Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices
US5759830A (en) * 1986-11-20 1998-06-02 Massachusetts Institute Of Technology Three-dimensional fibrous scaffold containing attached cells for producing vascularized tissue in vivo
US5567612A (en) * 1986-11-20 1996-10-22 Massachusetts Institute Of Technology Genitourinary cell-matrix structure for implantation into a human and a method of making
NZ229354A (en) 1988-07-01 1990-09-26 Becton Dickinson Co Treating polymer surfaces with a gas plasma and then applying a layer of endothelial cells to the surface
EP0363125A3 (en) 1988-10-03 1990-08-16 Hana Biologics Inc. Proliferated pancreatic endocrine cell product and process
US5837539A (en) * 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
WO1992019759A1 (en) 1991-04-25 1992-11-12 Chugai Seiyaku Kabushiki Kaisha Reconstituted human antibody against human interleukin 6 receptor
US5449383A (en) * 1992-03-18 1995-09-12 Chatelier; Ronald C. Cell growth substrates
GB9206861D0 (en) * 1992-03-28 1992-05-13 Univ Manchester Wound healing and treatment of fibrotic disorders
CA2114282A1 (en) * 1993-01-28 1994-07-29 Lothar Schilder Multi-layered implant
JP3525221B2 (ja) 1993-02-17 2004-05-10 味の素株式会社 免疫抑制剤
AU687386B2 (en) 1993-04-08 1998-02-26 Human Cell Cultures, Inc. Cell culturing method and medium
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
GB9310557D0 (en) * 1993-05-21 1993-07-07 Smithkline Beecham Plc Novel process and apparatus
TW257671B (es) * 1993-11-19 1995-09-21 Ciba Geigy
US6703017B1 (en) * 1994-04-28 2004-03-09 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US5834308A (en) * 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US6001647A (en) * 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US6083903A (en) * 1994-10-28 2000-07-04 Leukosite, Inc. Boronic ester and acid compounds, synthesis and uses
ES2170815T5 (es) 1994-12-29 2012-11-14 Chugai Seiyaku Kabushiki Kaisha Uso de un anticuerpo PM-1 o de un anticuerpo MH166 para potenciar el efecto antitumoral de cisplatino o carboplatino
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US7410773B2 (en) * 1995-02-02 2008-08-12 Ghazi Jaswinder Dhoot Method of preparing an undifferentiated cell
US5718922A (en) * 1995-05-31 1998-02-17 Schepens Eye Research Institute, Inc. Intravitreal microsphere drug delivery and method of preparation
US5908782A (en) * 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
US5681561A (en) 1995-06-07 1997-10-28 Life Medical Sciences, Inc. Compositions and methods for improving autologous fat grafting
US5969210A (en) * 1996-01-22 1999-10-19 Research Development Foundation Methods for the characterization of compounds which stimulate STF-1 expression in pancreatic islet cells
BR9808998A (pt) 1997-04-24 2000-08-08 Ortho Mcneil Pharm Inc Imidazóis substituìdos úteis no tratamento de doenças inflamatórias
AU8476698A (en) * 1997-07-03 1999-01-25 Osiris Therapeutics, Inc. Human mesenchymal stem cells from peripheral blood
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
DK1015576T3 (da) * 1997-09-16 2005-08-29 Egea Biosciences Llc Fremgangsmåde til fuldstændig kemisk syntese og aggregering af gener og genomer
AU1197699A (en) 1997-10-23 1999-05-10 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
ZA9811898B (en) * 1997-12-29 2000-06-28 Ortho Mcneil Pharm Inc Anti-Inflammatory Compounds.
CA2320040C (en) * 1998-03-18 2007-05-22 Osiris Therapeutics, Inc. Mesenchymal stem cells for prevention and treatment of immune responses in transplantation
MY132496A (en) * 1998-05-11 2007-10-31 Vertex Pharma Inhibitors of p38
US6413773B1 (en) 1998-06-01 2002-07-02 The Regents Of The University Of California Phosphatidylinositol 3-kinase inhibitors as stimulators of endocrine differentiation
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6610540B1 (en) 1998-11-18 2003-08-26 California Institute Of Technology Low oxygen culturing of central nervous system progenitor cells
US6413556B1 (en) * 1999-01-08 2002-07-02 Sky High, Llc Aqueous anti-apoptotic compositions
US6458593B1 (en) * 1999-01-21 2002-10-01 Vitro Diagnostics, Inc. Immortalized cell lines and methods of making the same
US6815203B1 (en) * 1999-06-23 2004-11-09 Joslin Diabetes Center, Inc. Methods of making pancreatic islet cells
US6333029B1 (en) * 1999-06-30 2001-12-25 Ethicon, Inc. Porous tissue scaffoldings for the repair of regeneration of tissue
US6306424B1 (en) * 1999-06-30 2001-10-23 Ethicon, Inc. Foam composite for the repair or regeneration of tissue
CA2385628A1 (en) 1999-09-27 2001-04-05 Ammon B. Peck Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US6685936B2 (en) * 1999-10-12 2004-02-03 Osiris Therapeutics, Inc. Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation
US20030082155A1 (en) 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
JP2003517592A (ja) * 1999-12-13 2003-05-27 ザ スクリプス リサーチ インスティチュート 膵島αおよびβ前駆体の同定ならびに単離のためのマーカー
US7005252B1 (en) * 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US6436704B1 (en) * 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6458589B1 (en) * 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
KR100947577B1 (ko) * 2000-06-26 2010-03-15 엔씨 메디컬 리서치 가부시키가이샤 신경계 세포로 분화할 수 있는 세포를 포함하는 세포분획
NZ524806A (en) * 2000-10-23 2006-03-31 Smithkline Beecham Corp Tri-substituted 8H-pyrido[2,3-d]pyrimidin-7-one compounds
ES2245994T3 (es) 2000-12-08 2006-02-01 Ortho-Mcneil Pharmaceutical, Inc. Compuestos macro-heterociclicos utilizados como inhibidores de quinasa.
IL156339A0 (en) 2000-12-08 2004-01-04 Ortho Mcneil Pharm Inc Indazolyl-substituted pyrroline compounds as kinase inhibitors
US6599323B2 (en) * 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
EP1366148A2 (en) * 2001-01-24 2003-12-03 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE DEPARTMENT OF HEALTH & HUMAN SERVICES Differentiation of stem cells to pancreatic endocrine cells
JP2004517932A (ja) * 2001-01-25 2004-06-17 ミレニアム・ファーマシューティカルズ・インコーポレイテッド ボロン酸化合物製剤
US6656488B2 (en) * 2001-04-11 2003-12-02 Ethicon Endo-Surgery, Inc. Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering
WO2002086107A2 (en) 2001-04-19 2002-10-31 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung A method for differentiating stem cells into insulin-producing cells
DE60231035D1 (de) 2001-04-24 2009-03-19 Ajinomoto Kk Stammzellen und verfahren zu deren trennung
CA2447015A1 (en) 2001-05-15 2002-11-21 Rappaport Family Institute For Research In The Medical Sciences Insulin producing cells derived from human embryonic stem cells
US6626950B2 (en) * 2001-06-28 2003-09-30 Ethicon, Inc. Composite scaffold with post anchor for the repair and regeneration of tissue
KR100418195B1 (ko) 2001-07-05 2004-02-11 주식회사 우리기술 전력케이블의 다중절연진단장치 및 그 방법
GB0117583D0 (en) * 2001-07-19 2001-09-12 Astrazeneca Ab Novel compounds
AU2002319780A1 (en) * 2001-08-06 2003-02-24 Bresagen, Ltd. Alternative compositions and methods for the culture of stem cells
US6617152B2 (en) * 2001-09-04 2003-09-09 Corning Inc Method for creating a cell growth surface on a polymeric substrate
EP1298201A1 (en) 2001-09-27 2003-04-02 Cardion AG Process for the production of cells exhibiting an islet-beta-cell-like state
WO2003033697A1 (en) 2001-10-18 2003-04-24 Ixion Biotechnology, Inc. Conversion of liver stem and progenitor cells to pancreatic functional cells
ATE438708T1 (de) 2001-11-15 2009-08-15 Childrens Medical Center Verfahren zur isolierung, expansion und differenzierung fötaler stammzellen aus chorionzotte, fruchtwasser und plazenta und therapeutische verwendungen davon
EP2305276A3 (en) * 2001-12-07 2011-09-21 Cytori Therapeutics, Inc. Processed lipoaspirate cells for use in therapy
CA2692325C (en) * 2001-12-07 2015-10-20 Geron Corporation Islet cells from human embryonic stem cells
AU2002218893A1 (en) 2001-12-21 2003-07-09 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability
US20050095703A1 (en) 2001-12-28 2005-05-05 Henrik Semb Method for the establishment of a pluripotent human blastocyst - derived stem cell line
US20030162290A1 (en) 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells
US20030180268A1 (en) 2002-02-05 2003-09-25 Anthony Atala Tissue engineered construct for supplementing or replacing a damaged organ
DE10214095C1 (de) * 2002-03-28 2003-09-25 Bernd Karl Friedrich Kremer Dedifferenzierte, programmierbare Stammzellen monozytären Ursprungs, sowie deren Herstellung und Verwendung
CA2482716A1 (en) * 2002-04-17 2003-10-23 Otsuka Pharmaceutical Co., Ltd. Method of forming pancreatic .beta. cells from mesenchymal cells
US20040161419A1 (en) * 2002-04-19 2004-08-19 Strom Stephen C. Placental stem cells and uses thereof
EP1506192B1 (en) 2002-05-08 2008-02-27 Janssen Pharmaceutica N.V. Substituted pyrroline kinase inhibitors
US20060003446A1 (en) * 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
CA2485862A1 (en) 2002-05-28 2003-12-11 Becton, Dickinson And Company Expansion and transdifferentiation of human acinar cells
US6987110B2 (en) * 2002-06-05 2006-01-17 Janssen Pharmaceutica N.V. Substituted pyrrolines as kinase inhibitors
GB0212976D0 (en) 2002-06-06 2002-07-17 Tonejet Corp Pty Ltd Ejection method and apparatus
CN1171991C (zh) 2002-07-08 2004-10-20 徐如祥 人神经干细胞的培养方法
US6877147B2 (en) * 2002-07-22 2005-04-05 Broadcom Corporation Technique to assess timing delay by use of layout quality analyzer comparison
US7838290B2 (en) * 2002-07-25 2010-11-23 The Scripps Research Institute Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith
US20040110287A1 (en) 2002-07-29 2004-06-10 Es Cell International Pte Ltd. Multi-step method for the differentiation of insulin positive, glucose responsive cells
WO2004016747A2 (en) 2002-08-14 2004-02-26 University Of Florida Bone marrow cell differentiation
EP1539928A4 (en) * 2002-09-06 2006-09-06 Amcyte Inc POSIOTIVE PANCREATIC ENDOCRINE PROGENITOR CELLS CD56 IN ADULT HUMAN BEINGS
US9969977B2 (en) * 2002-09-20 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
US20040062753A1 (en) * 2002-09-27 2004-04-01 Alireza Rezania Composite scaffolds seeded with mammalian cells
US20060252150A1 (en) 2002-11-08 2006-11-09 Linzhao Cheng Human embryonic stem cell cultures, and compositions and methods for growing same
US7144999B2 (en) * 2002-11-23 2006-12-05 Isis Pharmaceuticals, Inc. Modulation of hypoxia-inducible factor 1 alpha expression
US20060040385A1 (en) 2002-12-05 2006-02-23 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
PT2457999T (pt) 2002-12-16 2019-01-28 Technion Res & Dev Foundation Sistema de cultura para células estaminais pluripotentes
AU2004208606B2 (en) 2003-01-29 2009-09-24 Takeda Pharmaceutical Company Limited Process for producing coated preparation
RU2359671C2 (ru) 2003-01-29 2009-06-27 Такеда Фармасьютикал Компани Лимитед Способ получения препарата с покрытием
US20070155661A1 (en) 2003-02-14 2007-07-05 The Board Of Trustees Of The Leland Standord Junior University Methods and compositions for modulating the development of stem cells
WO2005045001A2 (en) 2003-02-14 2005-05-19 The Board Of Trustees Of The Leland Stanford Junior University Insulin-producing cells derived from stem cells
CA2520861A1 (en) 2003-03-27 2004-10-14 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic pathway
WO2004090110A2 (en) 2003-03-31 2004-10-21 Bresagen Inc. Compositions and methods for the control, differentiation and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway
US20090203141A1 (en) 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
PL1641917T3 (pl) * 2003-06-27 2016-11-30 Naprawa i regeneracja tkanek miękkich przy zastosowaniu komórek pozyskiwanych poporodowo
IL161903A0 (en) 2003-07-17 2005-11-20 Gamida Cell Ltd Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs
ITRM20030395A1 (it) 2003-08-12 2005-02-13 Istituto Naz Per Le Malattie Infettive Lazz Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero.
US20050042595A1 (en) 2003-08-14 2005-02-24 Martin Haas Banking of multipotent amniotic fetal stem cells
US7157275B2 (en) 2003-08-15 2007-01-02 Becton, Dickinson And Company Peptides for enhanced cell attachment and growth
US20060205072A1 (en) 2003-08-27 2006-09-14 Nobuko Uchida Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations
EP1696899A1 (en) 2003-12-17 2006-09-06 Allergan, Inc. Methods for treating retinoid responsive disorders using selective inhibitors of cyp26a and cyp26b
GB0329498D0 (en) 2003-12-19 2004-01-28 Novartis Ag Organic compounds
US20060030042A1 (en) * 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
US20050266554A1 (en) * 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
CN109628371B (zh) 2003-12-23 2021-02-19 维亚希特公司 定形内胚层
US7625753B2 (en) * 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
MX2009009225A (es) 2003-12-23 2009-09-28 Cythera Inc Endodermo definitivo.
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
TWI334443B (en) * 2003-12-31 2010-12-11 Ind Tech Res Inst Method of single cell culture of undifferentiated human embryonic stem cells
US7794704B2 (en) 2004-01-23 2010-09-14 Advanced Cell Technology, Inc. Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
WO2005071066A1 (en) 2004-01-23 2005-08-04 Board Of Regents, The University Of Texas System Methods and compositions for preparing pancreatic insulin secreting cells
WO2005080551A2 (en) 2004-02-12 2005-09-01 University Of Newcastle Upon Tyne Stem cells
JP4901471B2 (ja) 2004-02-19 2012-03-21 国立大学法人京都大学 体細胞核初期化物質のスクリーニング方法
CA2581424A1 (en) 2004-03-09 2005-09-22 Gang Xu Methods for generating insulin-producing cells
WO2005086845A2 (en) 2004-03-10 2005-09-22 Regents Of The University Of California Compositions and methods for growth of embryonic stem cells
WO2005090557A1 (ja) 2004-03-23 2005-09-29 Daiichi Asubio Pharma Co., Ltd. 多能性幹細胞の増殖方法
WO2005097980A2 (en) 2004-03-26 2005-10-20 Geron Corporation New protocols for making hepatocytes from embryonic stem cells
AU2005230832B2 (en) 2004-04-01 2010-11-11 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage
EP1740612B1 (en) 2004-04-27 2019-08-07 Viacyte, Inc. Pdx1 expressing endoderm
AU2005272078B2 (en) 2004-07-09 2011-04-14 Viacyte, Inc. Methods for identifying factors for differentiating definitive endoderm
EP1791952A4 (en) 2004-08-13 2008-06-11 Univ Georgia Res Found COMPOSITIONS AND METHODS OF SELF-RENEWAL AND DIFFERENTIATION IN HUMAN EMBRYONAL STEM CELLS
WO2006026473A2 (en) 2004-08-25 2006-03-09 University Of Georgia Research Foundation, Inc. METHODS AND COMPOSITIONS UTILIZING MYC AND GSK3ß TO MANIPULATE THE PLURIPOTENCY OF EMBRYONIC STEM CELLS
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
NZ553235A (en) 2004-09-08 2009-11-27 Wisconsin Alumni Res Found Culturing human pluripotent stem cells
KR101264940B1 (ko) 2004-09-08 2013-05-15 위스콘신 얼럼나이 리서어치 화운데이션 배아 줄기 세포용 배지 및 이의 배양물
WO2006079854A1 (en) 2005-01-28 2006-08-03 Novathera Ltd Methods for embryonic stem cell culture
AU2006210955A1 (en) 2005-01-31 2006-08-10 Es Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
US20060182724A1 (en) 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
SG160373A1 (en) 2005-03-04 2010-04-29 John Oaeneil Adult pancreatic derived stromal cells
GB0505970D0 (en) 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
ATE553198T1 (de) 2005-04-15 2012-04-15 Geron Corp Behandlung von krebs durch die kombinierte hemmung der proteasom- und telomeraseaktivitäten
CN100425694C (zh) 2005-04-15 2008-10-15 北京大学 诱导胚胎干细胞向胰腺细胞分化的方法
EP1875234B1 (en) 2005-04-26 2011-06-22 Aarhus Universitet Biosurface structure array
JP5092124B2 (ja) 2005-05-24 2012-12-05 国立大学法人 熊本大学 Ｅｓ細胞の分化誘導方法
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
MX2007015610A (es) 2005-06-10 2008-02-21 Irm Llc Compuestos que mantienen la fluripotencia de las celulas totipotentes embrionarias.
WO2006138433A2 (en) 2005-06-14 2006-12-28 The Regents Of The University Of California Induction of cell differentiation by class i bhlh polypeptides
EP1931764A1 (en) 2005-06-21 2008-06-18 GE Healthcare Bio-Sciences AB Method for cell culture
EP3599277A1 (en) 2005-06-22 2020-01-29 Asterias Biotherapeutics, Inc. Suspension culture of human embryonic stem cells
UA92608C2 (en) 2005-06-30 2010-11-25 Янссен Фармацевтика Н.В. Cyclic anilino - pyridinotriazines as gsk-3 inhibitors
WO2007016485A2 (en) 2005-07-29 2007-02-08 Athersys, Inc. Use of a gsk-3 inhibitor to maintain potency of cultured cells
US20090087907A1 (en) 2005-07-29 2009-04-02 Alice Pebay Compositions and Methods for Growth of Pluripotent Cells
WO2007025234A2 (en) 2005-08-26 2007-03-01 The Trustees Of Columbia University In The City Of New York Generation of pancreatic endocrine cells from primary duct cell cultures and methods of use for treatment of diabetes
US8476070B2 (en) 2005-08-29 2013-07-02 Technion Research & Development Foundation Limited Media for culturing stem cells
EP1943334A1 (en) 2005-09-02 2008-07-16 Agency for Science, Technology and Research Method of deriving progenitor cell line
GB2444686B (en) 2005-09-12 2010-08-25 Es Cell Int Pte Ltd Differentiation of pluripotent stem cells using p38 MAPK inhibitors or prostaglandins
AU2006304318B2 (en) 2005-10-14 2012-12-06 Regents Of The University Of Minnesota Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
AU2006305879B2 (en) 2005-10-27 2012-05-10 Viacyte, Inc. PDX1-expressing dorsal and ventral foregut endoderm
NZ569530A (en) 2005-12-13 2011-07-29 Univ Kyoto Nuclear reprogramming factor
WO2007082963A1 (es) 2006-01-18 2007-07-26 Fundación Instituto Valenciano De Infertilidad Líneas de células madre embrionarias humanas y métodos para usar las mismas
CN113088483A (zh) 2006-02-23 2021-07-09 维亚赛特公司 用于培养可分化细胞的组合物和方法
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
EP1999253B1 (en) 2006-03-02 2019-05-22 Viacyte, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
CA2984541C (en) 2006-04-28 2022-04-12 Lifescan, Inc. Differentiation of human embryonic stem cells
SE534150C2 (sv) 2006-05-02 2011-05-10 Wisconsin Alumni Res Found Förfarande för differentiering av stamceller till celler av den endodermala och pankreatiska utvecklingslinjen
US8685730B2 (en) 2006-05-02 2014-04-01 Wisconsin Alumni Research Foundation Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
WO2007139929A2 (en) 2006-05-25 2007-12-06 The Burnham Institute For Medical Research Methods for culture and production of single cell populations of human embryonic stem cells
AU2007254766A1 (en) * 2006-06-02 2007-12-13 University Of Georgia Research Foundation, Inc. Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems
CN101541953A (zh) 2006-06-02 2009-09-23 佐治亚大学研究基金会 通过从人胚胎干细胞获得的定形内胚层细胞的分化得到胰和肝内胚层细胞及组织
US8415153B2 (en) 2006-06-19 2013-04-09 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
CN100494359C (zh) 2006-06-23 2009-06-03 中日友好医院 神经干细胞三维立体培养体外扩增的方法
CA2893679C (en) 2006-06-26 2018-10-30 Lifescan, Inc. Conditioned medium for pluripotent stem cell culture
US20080003676A1 (en) 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
AU2007270069B2 (en) 2006-07-06 2013-05-16 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
WO2008013664A2 (en) 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
EP3441459B1 (en) 2006-08-02 2021-03-17 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
KR101331510B1 (ko) 2006-08-30 2013-11-20 재단법인서울대학교산학협력재단 저농도의 포도당을 함유하는 인간 배아줄기세포용 배지조성물 및 이를 이용한 인간 배아 줄기세포로부터 인슐린생산 세포 또는 세포괴로 분화시키는 방법, 그리고그로부터 유도된 인슐린 생산 세포 또는 세포괴
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
WO2008039521A2 (en) 2006-09-26 2008-04-03 Nmt Medical, Inc. Method for modifying a medical implant surface for promoting tissue growth
US8188124B2 (en) 2006-10-17 2012-05-29 Stiefel Laboratories, Inc. Talarazole metabolites
WO2008048647A1 (en) 2006-10-17 2008-04-24 Cythera, Inc. Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells
US8835163B2 (en) 2006-10-18 2014-09-16 The Board Of Trustees Of The University Of Illinois Embryonic-like stem cells derived from adult human peripheral blood and methods of use
EP2088190A4 (en) 2006-11-09 2011-01-05 Japan Government METHOD FOR THE CULTURE AND PASSING OF AN EMBRYONAL PRIMATIVE STEM CELL AND METHOD FOR INDUCING THE DIFFERENTIATION OF THE EMBRYONAL STEM CELL
US8217027B2 (en) 2006-12-21 2012-07-10 Abbott Laboratories Sphingosine-1-phosphate receptor agonist and antagonist compounds
TW200836749A (en) 2007-01-09 2008-09-16 Vioquest Pharmaceuticals Inc Compositions including triciribine and bortezomib and derivatives thereof and methods of use thereof
CA2676044C (en) 2007-01-30 2019-10-22 University Of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (mmc)
GB0703188D0 (en) 2007-02-19 2007-03-28 Roger Land Building Large scale production of stem cells
US20090053182A1 (en) 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
US8815585B2 (en) 2007-06-29 2014-08-26 Cellular Dynamics International, Inc. Automated method and apparatus for embryonic stem cell culture
KR20160054032A (ko) 2007-07-01 2016-05-13 라이프스캔, 인코포레이티드 단일 다분화성 줄기 세포 배양
EP2185695B1 (en) 2007-07-18 2015-02-25 Lifescan, Inc. Differentiation of human embryonic stem cells
CN101952415B (zh) * 2007-07-31 2017-06-27 生命扫描有限公司 人胚胎干细胞的分化
WO2009048675A1 (en) 2007-07-31 2009-04-16 Lifescan, Inc. Pluripotent stem cell differentiation by using human feeder cells
MX2010002179A (es) 2007-08-24 2010-04-27 Stichting Het Nl Kanker I Composicion para el tratamiento de enfermedades neoplasicas.
US20110151447A1 (en) 2007-11-06 2011-06-23 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells from non-embryonic human cells
EP2229434B1 (en) 2007-11-27 2011-09-07 Lifescan, Inc. Differentiation of human embryonic stem cells
SG154367A1 (en) 2008-01-31 2009-08-28 Es Cell Int Pte Ltd Method of differentiating stem cells
WO2009096049A1 (ja) 2008-02-01 2009-08-06 Kyoto University 人工多能性幹細胞由来分化細胞
US20100330677A1 (en) 2008-02-11 2010-12-30 Cambridge Enterprise Limited Improved Reprogramming of Mammalian Cells, and Cells Obtained
BR122017025207B1 (pt) 2008-02-21 2021-03-16 Centocor Ortho Biotech Inc superfície que faz parte de um recipiente ou matriz destinada para uso em uma cultura de células ou análises, desprovida de uma camada de células alimentadoras e desprovida de uma camada adsorvente
WO2009110215A1 (ja) 2008-03-03 2009-09-11 独立行政法人 科学技術振興機構 繊毛細胞の分化誘導方法
SG188918A1 (es) 2008-03-17 2013-04-30 Agency Science Tech & Res
RU2359030C1 (ru) 2008-03-19 2009-06-20 Общество С Ограниченной Ответственностью "Лаборатория Клеточных Технологий" Способ получения эндотелиальных клеток из эмбриональных стволовых клеток человека (варианты)
US8338170B2 (en) * 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
EP2727998B1 (en) * 2008-04-21 2019-06-12 Viacyte, Inc. Methods for purifying pancreatic endoderm cells derived from human embryonic stem cells
WO2009132083A2 (en) 2008-04-22 2009-10-29 President And Fellows Of Harvard College Compositions and methods for promoting the generation of pdx1+ pancreatic cells
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
US20090298178A1 (en) 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
WO2009154606A1 (en) 2008-06-03 2009-12-23 Cythera, Inc. Growth factors for production of definitive endoderm
DE102008032236A1 (de) 2008-06-30 2010-04-01 Eberhard-Karls-Universität Tübingen Isolierung und/oder Identifizierung von Stammzellen mit adipozytärem, chondrozytärem und pankreatischem Differenzierungspotential
CA2729121C (en) 2008-06-30 2019-04-09 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
US20100028307A1 (en) 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
WO2010022395A2 (en) 2008-08-22 2010-02-25 President And Fellows Of Harvard College Methods of reprogramming cells
KR101798474B1 (ko) 2008-10-31 2017-11-16 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 췌장 내분비 계통으로의 분화
EP2346988B1 (en) 2008-10-31 2017-05-31 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
EP3550012A1 (en) 2008-11-04 2019-10-09 ViaCyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
CA2743641C (en) 2008-11-14 2024-03-26 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
EP2366021B1 (en) 2008-11-20 2017-08-02 Janssen Biotech, Inc. Pluripotent stem cell culture on micro-carriers
EP2356218B1 (en) 2008-12-05 2017-05-03 INSERM (Institut National de la Santé et de la Recherche Médicale) Method and medium for neural differentiation of pluripotent cells
MX340952B (es) 2009-07-20 2016-07-29 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
CA2768643C (en) 2009-07-20 2018-09-18 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
GB2485113B (en) 2009-07-20 2016-12-28 Janssen Biotech Inc Differentiation of human embryonic stem cells into cells of the pancreatic endoderm lineage
US10119120B2 (en) 2009-08-12 2018-11-06 Kyoto University Method for inducing differentiation of pluripotent stem cells into neural precursor cells
AU2010319921A1 (en) 2009-10-29 2012-05-17 Janssen Biotech Inc. Pluripotent stem cells
CA2783437C (en) 2009-11-12 2020-08-04 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
WO2011079017A2 (en) 2009-12-23 2011-06-30 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
US9133439B2 (en) 2009-12-23 2015-09-15 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
US8932853B2 (en) 2009-12-29 2015-01-13 Takeda Pharmaceutical Company Limited Method for manufacturing pancreatic-hormone-producing cells
CN107189979B (zh) * 2010-03-01 2021-05-04 詹森生物科技公司 纯化衍生自多能干细胞的细胞的方法
WO2011108993A1 (en) 2010-03-02 2011-09-09 National University Of Singapore Culture additives to boost stem cell proliferation and differentiation response
CN105176930B (zh) 2010-03-31 2021-05-04 斯克里普斯研究所 重编程细胞
EP2563908B1 (en) 2010-04-25 2019-01-09 Icahn School of Medicine at Mount Sinai Generation of anterior foregut endoderm from pluripotent cells
BR112012028855A2 (pt) 2010-05-12 2015-09-22 Janssen Biotech Inc diferenciação das células-tronco embrionárias humanas
EP2582795B1 (en) 2010-06-15 2017-04-12 Kyoto University Method for selecting human induced pluripotent stem cells
US9085757B2 (en) 2010-06-17 2015-07-21 Regents Of The University Of Minnesota Production of insulin producing cells
SG187699A1 (en) 2010-08-05 2013-03-28 Wisconsin Alumni Res Found Simplified basic media for human pluripotent cell culture
JP6133776B2 (ja) 2010-08-31 2017-05-24 ヤンセン バイオテツク，インコーポレーテツド 多能性幹細胞の分化
MY177150A (en) 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013055834A2 (en) 2011-10-11 2013-04-18 The New York Stem Cell Foundation Er stress relievers in beta cell protection
US8951798B2 (en) 2011-10-13 2015-02-10 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
US9670463B2 (en) 2011-10-14 2017-06-06 Children's Medical Center Corporation Inhibition and enhancement of reprogramming by chromatin modifying enzymes
HK1203552A1 (en) 2011-12-22 2015-10-30 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
US10519422B2 (en) 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
RU2018108850A (ru) 2012-06-08 2019-02-26 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки
US20150247123A1 (en) 2012-09-03 2015-09-03 Novo Nordisk A/S Generation of pancreatic endoderm from Pluripotent Stem cells using small molecules
KR102036780B1 (ko) 2012-12-31 2019-10-25 얀센 바이오테크 인코포레이티드 Hb9 조절제를 사용하는 인간 배아 줄기세포의 췌장 내분비 세포로의 분화
WO2014127164A1 (en) 2013-02-14 2014-08-21 Allergan, Inc. Substituted dihydropyrazoles as sphingosine receptor modulators
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
CA2906643A1 (en) 2013-03-15 2014-09-25 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof

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