ATE481499T1 - Cocktail von nukleaseinhibitoren - Google Patents
Cocktail von nukleaseinhibitorenInfo
- Publication number
- ATE481499T1 ATE481499T1 AT00966924T AT00966924T ATE481499T1 AT E481499 T1 ATE481499 T1 AT E481499T1 AT 00966924 T AT00966924 T AT 00966924T AT 00966924 T AT00966924 T AT 00966924T AT E481499 T1 ATE481499 T1 AT E481499T1
- Authority
- AT
- Austria
- Prior art keywords
- nuclease
- antibodies
- inhibitors
- antibody
- nuclease inhibitors
- Prior art date
Links
- 101710163270 Nuclease Proteins 0.000 title abstract 10
- 239000003112 inhibitor Substances 0.000 title abstract 7
- 238000000338 in vitro Methods 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 229940122426 Nuclease inhibitor Drugs 0.000 abstract 2
- 102000016911 Deoxyribonucleases Human genes 0.000 abstract 1
- 108010053770 Deoxyribonucleases Proteins 0.000 abstract 1
- 102000006382 Ribonucleases Human genes 0.000 abstract 1
- 108010083644 Ribonucleases Proteins 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000002906 microbiologic effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
- G01N2333/922—Ribonucleases (RNAses); Deoxyribonucleases (DNAses)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Enzymes And Modification Thereof (AREA)
- Jellies, Jams, And Syrups (AREA)
- Alcoholic Beverages (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15587499P | 1999-09-24 | 1999-09-24 | |
| PCT/US2000/026485 WO2001021830A1 (en) | 1999-09-24 | 2000-09-25 | Nuclease inhibitor cocktail |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE481499T1 true ATE481499T1 (de) | 2010-10-15 |
Family
ID=22557124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00966924T ATE481499T1 (de) | 1999-09-24 | 2000-09-25 | Cocktail von nukleaseinhibitoren |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US6664379B1 (de) |
| EP (1) | EP1220944B1 (de) |
| AT (1) | ATE481499T1 (de) |
| DE (1) | DE60044977D1 (de) |
| WO (1) | WO2001021830A1 (de) |
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| US7264932B2 (en) * | 1999-09-24 | 2007-09-04 | Applera Corporation | Nuclease inhibitor cocktail |
| EP1220944B1 (de) * | 1999-09-24 | 2010-09-15 | Ambion, Inc. | Cocktail von nukleaseinhibitoren |
| ATE373102T1 (de) * | 2001-03-08 | 2007-09-15 | Invitrogen Corp | Verbessertes in-vitro-synthesesystem |
| EP1300465A1 (de) * | 2001-10-08 | 2003-04-09 | Agrobiogen GmbH Biotechnologie | Verfahren und Vorrichtung zur Isolierung von RNS-Proben |
| EP2290098A1 (de) * | 2002-01-28 | 2011-03-02 | Ambion, Inc. | Herstellung biologische Rohderivaten unter Einzetsung Proteasen, geeignet für cDNA Synthese |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| DK2143803T3 (en) | 2002-09-03 | 2016-08-01 | Quanta Biosciences Inc | Improved compositions and methods for cDNA synthesis |
| CA2445204C (en) | 2002-10-16 | 2014-08-12 | Streck Laboratories, Inc. | Method and device for collecting and preserving cells for analysis |
| US7291459B2 (en) * | 2002-12-10 | 2007-11-06 | University Of Alabama At Huntsville | Nucleic acid detector and method of detecting targets within a sample |
| WO2004076640A2 (en) * | 2003-02-25 | 2004-09-10 | Ambion, Inc. | Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same |
| CN1878866B (zh) † | 2003-07-25 | 2012-08-01 | 安比恩股份有限公司 | 用于从固定的样品中制备rna的方法和组合物 |
| US20050208501A1 (en) | 2004-03-16 | 2005-09-22 | Ambion, Inc. | Process and reagents for extraction of RNA from fractionated blood leukocytes |
| US8426126B2 (en) * | 2004-03-18 | 2013-04-23 | Applied Biosystems, Llc | Modified surfaces as solid supports for nucleic acid purification |
| US8460684B2 (en) | 2004-03-19 | 2013-06-11 | Wisconsin Alumni Research Foundation | Nuclease inhibitors and methods for their use |
| US20050277121A1 (en) * | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
| US20080004436A1 (en) * | 2004-11-15 | 2008-01-03 | Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science | Directed Evolution and Selection Using in Vitro Compartmentalization |
| US20060188892A1 (en) * | 2005-02-18 | 2006-08-24 | Ambion, Inc. | Enzymatic digestion of tissue |
| DE102005015005A1 (de) * | 2005-04-01 | 2006-10-05 | Qiagen Gmbh | Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe |
| US20070015165A1 (en) * | 2005-07-13 | 2007-01-18 | Sigma-Aldrich Co. | Method for the isolation of RNA from biological sources |
| EP2066686A4 (de) | 2006-10-06 | 2010-07-14 | Dna Genotek Inc | Stabilisierungszusammensetzungen und verfahren zur extraktion von ribonukleinsäure |
| US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
| NZ592013A (en) * | 2008-10-06 | 2012-06-29 | Symphogen As | Method for identifying and selecting drug candidates for combinatorial drug products |
| EP2175020A1 (de) * | 2008-10-13 | 2010-04-14 | Roche Diagnostics GmbH | Verringerung der RNase-Aktivität in komplexen flüssigen Proben |
| CA2739705A1 (en) * | 2008-10-30 | 2010-05-06 | Yissum Research Development Company Of The Hebrew University Of Jerusale M Ltd. | Efficient expression of truncated human rnaset2 in e. coli |
| US8211637B2 (en) | 2008-12-19 | 2012-07-03 | Life Technologies Corporation | Proteinase K inhibitors, methods and compositions therefor |
| US11634747B2 (en) * | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| WO2010096323A1 (en) | 2009-02-18 | 2010-08-26 | Streck, Inc. | Preservation of cell-free nucleic acids |
| WO2011008553A1 (en) * | 2009-06-29 | 2011-01-20 | Life Technologies Corporation | Rna sample stabilization in the presence of a transitional metal |
| WO2011057184A1 (en) | 2009-11-09 | 2011-05-12 | Streck, Inc. | Stabilization of rna in and extracting from intact cells within a blood sample |
| CN102243154B (zh) * | 2011-04-02 | 2016-05-11 | 中山大学附属第一医院 | 一种周围神经切片染色的方法 |
| WO2012151391A2 (en) | 2011-05-04 | 2012-11-08 | Streck, Inc. | Inactivated virus compositions and methods of preparing such compositions |
| CN103890163B (zh) | 2011-06-19 | 2016-09-14 | 阿博根公司 | 用于样品采集的装置、溶液和方法 |
| WO2013055898A1 (en) * | 2011-10-14 | 2013-04-18 | The Trustees Of The University Of Pennsylvania | Methods for enhancing the efficacy of microrna mediated interference |
| US9604213B2 (en) | 2012-02-13 | 2017-03-28 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| US11648561B2 (en) | 2012-02-13 | 2023-05-16 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| US11485968B2 (en) | 2012-02-13 | 2022-11-01 | Neumodx Molecular, Inc. | Microfluidic cartridge for processing and detecting nucleic acids |
| US9637775B2 (en) | 2012-02-13 | 2017-05-02 | Neumodx Molecular, Inc. | System and method for processing biological samples |
| EP2814942B1 (de) | 2012-02-13 | 2024-07-03 | Neumodx Molecular, Inc. | Mikrofluidische kartusche zur verarbeitung und zum nachweis von nucleinsäuren |
| US9040679B2 (en) | 2012-04-30 | 2015-05-26 | General Electric Company | Methods and compositions for extraction and storage of nucleic acids |
| US9044738B2 (en) | 2012-04-30 | 2015-06-02 | General Electric Company | Methods and compositions for extraction and storage of nucleic acids |
| US9040675B2 (en) | 2012-04-30 | 2015-05-26 | General Electric Company | Formulations for nucleic acid stabilization on solid substrates |
| US9480966B2 (en) | 2012-04-30 | 2016-11-01 | General Electric Company | Substrates and methods for collection, stabilization and elution of biomolecules |
| JP6236864B2 (ja) * | 2012-06-11 | 2017-11-29 | 東ソー株式会社 | 癌の検出方法及び膵臓特異的リボヌクレアーゼ１を認識する抗体 |
| BR112015005718A2 (pt) * | 2012-09-19 | 2017-07-04 | Beckman Coulter Inc | uso de íons divalentes, proteases, detergentes, e ph baixo na extração de ácidos nucleicos |
| US20140120544A1 (en) | 2012-10-25 | 2014-05-01 | Neumodx Molecular, Inc. | Method and materials for isolation of nucleic acid materials |
| EP3024323B1 (de) | 2013-07-24 | 2022-10-19 | Streck, Inc. | Zusammensetzungen und verfahren zur stabilisierung zirkulierender tumorzellen |
| EP3102704B1 (de) | 2014-02-07 | 2020-02-12 | University of Iowa Research Foundation | Oligonukleotidbasierte sonden und verfahren zum nachweis von mikroben |
| EP3825411A1 (de) | 2014-06-18 | 2021-05-26 | Clear Gene, Inc. | Verfahren, zusammensetzungen und vorrichtungen zur schnellanalyse von biologischen markern |
| US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
| CN108473932B (zh) | 2015-09-09 | 2022-07-15 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
| US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
| AU2016369603A1 (en) | 2015-12-18 | 2018-07-05 | Clear Gene, Inc. | Methods, compositions, kits and devices for rapid analysis of biological markers |
| WO2018022991A1 (en) | 2016-07-29 | 2018-02-01 | Streck, Inc. | Suspension composition for hematology analysis control |
| US12275994B2 (en) | 2017-06-22 | 2025-04-15 | Clear Gene, Inc. | Methods and compositions for the analysis of cancer biomarkers |
| US12378591B2 (en) | 2017-09-29 | 2025-08-05 | University Of Iowa Research Foundation | Digital nuclease detection compositions and methods |
| EP3697209B1 (de) | 2017-10-19 | 2024-07-24 | Streck LLC | Zusammensetzungen zur hämolyse- und gerinnungsregulierung sowie stabilisierung von extrazellulären vesikeln |
| WO2022015768A1 (en) * | 2020-07-13 | 2022-01-20 | The Board Of Trustees Of The Leland Stanford Junior University | Systems and methods to enhance rna transcription and uses thereof |
| CN114249829B (zh) * | 2021-12-20 | 2022-09-20 | 南京诺唯赞生物科技股份有限公司 | 一种RNase抑制剂的单克隆抗体及其制备方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58116498A (ja) | 1981-12-28 | 1983-07-11 | Takeda Chem Ind Ltd | Ｉｌ‐２をコードする新規伝令ｒｎａの製造法 |
| US5278062A (en) | 1987-04-03 | 1994-01-11 | Amgen | Proteolytic enzymes |
| US5151504A (en) * | 1989-11-17 | 1992-09-29 | E. R. Squibb & Sons, Inc. | Method for purification of monoclonal antibodies |
| US5470971A (en) | 1991-03-11 | 1995-11-28 | The University Of Medicine And Dentistry Of New Jersey | Stress-induced proteins, genes coding therefor, transformed cells of organisms, methods and applications |
| US5346994A (en) | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| US6110968A (en) * | 1995-12-26 | 2000-08-29 | The Picower Institute For Medical Research | Methods for treatment predicated on the presence of advanced glycosylation endproducts in tobacco and its combustion byproducts |
| US5973137A (en) * | 1996-02-13 | 1999-10-26 | Gentra Systems, Inc. | Low pH RNA isolation reagents, method, and kit |
| KR100361892B1 (ko) | 1996-05-02 | 2003-01-24 | 더 프록터 앤드 갬블 캄파니 | 분산된계면활성제착체를포함하는국소조성물 |
| CA2253455A1 (en) | 1996-05-02 | 1997-11-06 | The Procter & Gamble Company | Topical compositions comprising dispersed surfactant complex |
| US5968784A (en) | 1997-01-15 | 1999-10-19 | Chugai Pharmaceutical Co., Ltd. | Method for analyzing quantitative expression of genes |
| US5972613A (en) * | 1997-12-09 | 1999-10-26 | The Perkin-Elmer Corporation | Methods of nucleic acid isolation |
| US6869604B1 (en) * | 1998-03-27 | 2005-03-22 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Recombinant anti-tumor RNAse |
| US7264932B2 (en) | 1999-09-24 | 2007-09-04 | Applera Corporation | Nuclease inhibitor cocktail |
| EP1220944B1 (de) | 1999-09-24 | 2010-09-15 | Ambion, Inc. | Cocktail von nukleaseinhibitoren |
| US6793019B2 (en) | 2002-07-10 | 2004-09-21 | Abb Offshore Systems, Inc. | Tapered ramp positive lock latch mechanism |
| US7021540B2 (en) * | 2002-07-30 | 2006-04-04 | Canon Kabushiki Kaisha | Storage medium mounting/dismounting mechanism and information processing apparatus |
| WO2004076640A2 (en) | 2003-02-25 | 2004-09-10 | Ambion, Inc. | Small-molecule inhibitors of angiogenin and rnases and in vivo and in vitro methods of using same |
-
2000
- 2000-09-25 EP EP00966924A patent/EP1220944B1/de not_active Expired - Lifetime
- 2000-09-25 US US09/669,301 patent/US6664379B1/en not_active Expired - Lifetime
- 2000-09-25 AT AT00966924T patent/ATE481499T1/de not_active IP Right Cessation
- 2000-09-25 WO PCT/US2000/026485 patent/WO2001021830A1/en active Application Filing
- 2000-09-25 DE DE60044977T patent/DE60044977D1/de not_active Expired - Lifetime
-
2003
- 2003-09-30 US US10/675,860 patent/US7163793B2/en not_active Expired - Lifetime
-
2006
- 2006-12-06 US US11/567,651 patent/US7445901B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20070148174A1 (en) | 2007-06-28 |
| US6664379B1 (en) | 2003-12-16 |
| US7163793B2 (en) | 2007-01-16 |
| EP1220944A1 (de) | 2002-07-10 |
| EP1220944B1 (de) | 2010-09-15 |
| DE60044977D1 (de) | 2010-10-28 |
| WO2001021830A1 (en) | 2001-03-29 |
| US7445901B2 (en) | 2008-11-04 |
| EP1220944A4 (de) | 2005-06-22 |
| US20050026284A1 (en) | 2005-02-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |