AU714791B2 - Improvements in or relating to gene expression - Google Patents

Improvements in or relating to gene expression Download PDF

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Publication number: AU714791B2
Authority: AU; Australia
Prior art keywords: sequence; gal4; plant; gene; interest
Prior art date: 1996-02-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Ceased

Application number

AU18014/97A

Other languages

English (en)

Other versions

AU1801497A (en

Inventor

James Phillip Haseloff

Sarah Hodge

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

BTG International Ltd

Original Assignee

British Technology Group Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-02-14

Filing date

1997-02-14

Publication date

2000-01-13

1997-02-14 Application filed by British Technology Group Ltd filed Critical British Technology Group Ltd

1997-09-02 Publication of AU1801497A publication Critical patent/AU1801497A/en

2000-01-13 Application granted granted Critical

2000-01-13 Publication of AU714791B2 publication Critical patent/AU714791B2/en

2017-02-14 Anticipated expiration legal-status Critical

Status Ceased legal-status Critical Current

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers

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Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Chemical & Material Sciences (AREA)
General Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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AU18014/97A 1996-02-14 1997-02-14 Improvements in or relating to gene expression Ceased AU714791B2 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
GB9603069		1996-02-14
GBGB9603069.7A GB9603069D0 (en)	1996-02-14	1996-02-14	Improvements in or relating to gene expression
PCT/GB1997/000406 WO1997030164A1 (fr)	1996-02-14	1997-02-14	Ameliorations relatives a l'expression de genes

Publications (2)

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AU1801497A AU1801497A (en)	1997-09-02
AU714791B2 true AU714791B2 (en)	2000-01-13

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AU18014/97A Ceased AU714791B2 (en)	1996-02-14	1997-02-14	Improvements in or relating to gene expression

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EP (1)	EP0880593A1 (fr)
JP (1)	JP2001501805A (fr)
CN (1)	CN1216067A (fr)
AU (1)	AU714791B2 (fr)
CA (1)	CA2246414A1 (fr)
GB (1)	GB9603069D0 (fr)
NZ (1)	NZ331220A (fr)
WO (1)	WO1997030164A1 (fr)
ZA (1)	ZA971272B (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5968793A (en) *	1997-06-24	1999-10-19	E. I. Du Pont De Nemours And Company	Specific gene activation by chimeric Gal4 transcription factors in stable transgenic plants
US6087166A (en) *	1997-07-03	2000-07-11	Basf Aktiengesellschaft	Transcriptional activators with graded transactivation potential
CA2205888A1 (fr)	1997-07-11	1999-01-11	John J. Priatel	Piege de complementation
AU745867B2 (en) *	1997-11-26	2002-04-11	Syngenta Participations Ag	Method and compositions useful for the activation of silent transgenes
CA2224475A1 (fr) *	1998-02-25	1999-08-25	University Of British Columbia	Collecteur des facteurs de transcription et d'interaction de proteine
US6482646B1 (en) *	1998-11-06	2002-11-19	E. I. Du Pont De Nemours And Company	Plant proteins that interact with nuclear matrix proteins and function as transcriptional activators
CA2316036A1 (fr)	1999-08-27	2001-02-27	Keqiang Wu	Regulation de l'expression genetique chez des vegetaux
AU7705500A (en) *	1999-09-20	2001-04-24	Center For The Application Of Molecular Biology To International Agriculture	Methods for site-associated modification of gene activity and nucleic acid structure
EP2161340A3 (fr)	1999-12-16	2010-09-22	CropDesign N.V.	Transfert optimisé d'ADN-T et vecteurs associés
US6977293B1 (en)	2000-11-03	2005-12-20	Ceres, Inc.	Chimeric polypeptides
GB0122828D0 (en) *	2001-09-21	2001-11-14	Univ Cambridge Tech	Gene expression construct
GB0125784D0 (en) *	2001-10-26	2001-12-19	Syngenta Ltd	Novel expression system
US10677785B2 (en)	2010-05-24	2020-06-09	Plant Response, Inc.	Reporter constructs for compound screening
WO2011149843A1 (fr)	2010-05-24	2011-12-01	Mendel Biotechnology Inc.	Nouvelles constructions rapporteurs pour criblage de composés
US9612235B2 (en)	2012-04-05	2017-04-04	Koch Biological Solutions, Llc	Herbicidal compound screening
US9101100B1 (en)	2014-04-30	2015-08-11	Ceres, Inc.	Methods and materials for high throughput testing of transgene combinations
CN109957842B (zh) *	2019-02-11	2022-06-24	王纪武	一种用模块化设计构建双系统表达质粒文库的方法

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* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0428572A1 (fr) *	1988-07-29	1991-05-29	Washington University School Of Medicine	Production de polypeptides pour usage commercial au moyen d'un tissu d'endosperme transforme genetiquement
NZ230375A (en) *	1988-09-09	1991-07-26	Lubrizol Genetics Inc	Synthetic gene encoding b. thuringiensis insecticidal protein
DK0413019T3 (da) *	1989-02-24	2001-11-12	Monsanto Technology Llc	Syntetiske plantegener og fremgangsmåde til fremstilling af disse
US5723747A (en) *	1991-02-22	1998-03-03	Calgene, Inc.	Wax esters in transformed plants
US5409823A (en) *	1992-09-24	1995-04-25	Ciba-Geigy Corporation	Methods for the production of hybrid seed
GB9502456D0 (en) *	1995-02-08	1995-03-29	Univ Warwick	Control of genes in transgenic plants

1996
- 1996-02-14 GB GBGB9603069.7A patent/GB9603069D0/en active Pending
1997
- 1997-02-14 NZ NZ331220A patent/NZ331220A/en unknown
- 1997-02-14 ZA ZA9701272A patent/ZA971272B/xx unknown
- 1997-02-14 JP JP09529099A patent/JP2001501805A/ja active Pending
- 1997-02-14 CN CN97193684A patent/CN1216067A/zh active Pending
- 1997-02-14 AU AU18014/97A patent/AU714791B2/en not_active Ceased
- 1997-02-14 EP EP97903457A patent/EP0880593A1/fr not_active Withdrawn
- 1997-02-14 WO PCT/GB1997/000406 patent/WO1997030164A1/fr not_active Application Discontinuation
- 1997-02-14 CA CA002246414A patent/CA2246414A1/fr not_active Abandoned

Also Published As

Publication number	Publication date
NZ331220A (en)	2000-02-28
CA2246414A1 (fr)	1997-08-21
GB9603069D0 (en)	1996-04-10
JP2001501805A (ja)	2001-02-13
AU1801497A (en)	1997-09-02
CN1216067A (zh)	1999-05-05
ZA971272B (en)	1997-08-27
EP0880593A1 (fr)	1998-12-02
WO1997030164A1 (fr)	1997-08-21

Publication	Publication Date	Title
AU714791B2 (en)	2000-01-13	Improvements in or relating to gene expression
Johnson et al.	2005	Spatial control of transgene expression in rice (Oryza sativa L.) using the GAL4 enhancer trapping system
US5187267A (en)	1993-02-16	Plant proteins, promoters, coding sequences and use
JP4424756B2 (ja)	2010-03-03	組織特異的プロモーターを用いたトランスジェニック植物およびその作出方法
AU737966B2 (en)	2001-09-06	Sugarcane bacilliform virus promoter
US6255558B1 (en)	2001-07-03	Gene expression
US6391639B1 (en)	2002-05-21	Plant and viral promoters
Chen et al.	1998	Gene transfer via pollen‐tube pathway for anti‐fusarium wilt in watermelon
WO1997044448A9 (fr)	1998-03-26	Elements promoteurs conferant une expression genique dans les racines de maniere preferentielle.
US6489462B1 (en)	2002-12-03	Sugarcane bacilliform virus promoter
Lusardi et al.	1994	An approach towards genetically engineered cell fate mapping in maize using the Lc gene as a visible marker: transactivation capacity of Lc vectors in differentiated maize cells and microinjection of Lc vectors into somatic embryos and shoot apical meristems
CN110066327B (zh)	2021-01-01	蛋白质TaWRKY13在调控植物抗逆性中的应用
EP1461438B1 (fr)	2006-12-27	Construction d'expression de genes
AU2002324236A1 (en)	2003-06-05	Gene expression construct
HUT68066A (en)	1995-05-29	Transgenic wheat
CN101113452B (zh)	2010-06-09	植物花特异性启动子及其应用
US10047369B2 (en)	2018-08-14	Citrus derived transcription and translation regulatory elements for tissue specific expression
KR100740694B1 (ko)	2007-07-18	색소체 게놈 내에 삽입된 외래유전자의 이차 재조합을방지하기 위한 벡터개발
AU729274B2 (en)	2001-02-01	Plant and viral promoters
CN113604476A (zh)	2021-11-05	水稻剪接体gs3.2在调控水稻籽粒性状中的应用
Carpenter	1991	Promoter analysis of two genes coding for alpha-tubulin in Arabidopsis thaliana: pollen-preferential versus semi-constitutive expression in transgenic plants
KR20020057944A (ko)	2002-07-12	Ｍｉｔｅ-유사 인자 및 전사 활성화 인자
Bilgin	1999	Utilization of Transgenic Maize Plants for Functional Analysis of a Histone Promoter and Host-Geminivirus Interactions
JPH05153981A (ja)	1993-06-22	アミロース含量の改変されたイネ科植物およびその製造法
US20080256670A1 (en)	2008-10-16	AtRSp gene promoters

Legal Events

Date	Code	Title	Description
2000-05-18	FGA	Letters patent sealed or granted (standard patent)
2002-09-12	MK14	Patent ceased section 143(a) (annual fees not paid) or expired