CA2203143A1 - Sondes de microsatellites composes pour la detection de polymorphismes genetiques - Google Patents
Sondes de microsatellites composes pour la detection de polymorphismes genetiquesInfo
- Publication number
- CA2203143A1 CA2203143A1 CA 2203143 CA2203143A CA2203143A1 CA 2203143 A1 CA2203143 A1 CA 2203143A1 CA 2203143 CA2203143 CA 2203143 CA 2203143 A CA2203143 A CA 2203143A CA 2203143 A1 CA2203143 A1 CA 2203143A1
- Authority
- CA
- Canada
- Prior art keywords
- primer
- ssr
- dna
- amplification
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 235000011056 potassium acetate Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229920002477 rna polymer Polymers 0.000 description 1
- 238000010845 search algorithm Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000037432 silent mutation Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- -1 succinimidyl esters Chemical class 0.000 description 1
- 150000003461 sulfonyl halides Chemical class 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 238000007862 touchdown PCR Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- NLIVDORGVGAOOJ-MAHBNPEESA-M xylene cyanol Chemical compound [Na+].C1=C(C)C(NCC)=CC=C1C(\C=1C(=CC(OS([O-])=O)=CC=1)OS([O-])=O)=C\1C=C(C)\C(=[NH+]/CC)\C=C/1 NLIVDORGVGAOOJ-MAHBNPEESA-M 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne des microsatellites composés qui sont utilisés en tant que sondes d'autofixation pour la recherche de génomes dans le but de détecter des polymorphismes. Les sondes de microsatellites composées préférées sont des sondes de dinucléotides composés parfaits dans lesquelles les paires de dinucléotides adjacents contiennent un nucléotide commun qui est en phase sur la jonction du composé. Dans un mode de réalisation utile, ces sondes composées en phase ont été adaptées à d'autres dosages d'investigation connus y compris les variations des dosages AFLP.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34645694A | 1994-11-28 | 1994-11-28 | |
| US08/346,456 | 1994-11-28 | ||
| PCT/US1995/015150 WO1996017082A2 (fr) | 1994-11-28 | 1995-11-21 | Sondes de microsatellites composes pour la detection de polymorphismes genetiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2203143A1 true CA2203143A1 (fr) | 1996-06-06 |
Family
ID=29405876
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2203143 Abandoned CA2203143A1 (fr) | 1994-11-28 | 1995-11-21 | Sondes de microsatellites composes pour la detection de polymorphismes genetiques |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2203143A1 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114128649A (zh) * | 2021-11-02 | 2022-03-04 | 武汉市农业科学院 | 一种团头鲂与黑尾近红鲌间远缘杂交子代的选择育种方法 |
-
1995
- 1995-11-21 CA CA 2203143 patent/CA2203143A1/fr not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114128649A (zh) * | 2021-11-02 | 2022-03-04 | 武汉市农业科学院 | 一种团头鲂与黑尾近红鲌间远缘杂交子代的选择育种方法 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |