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CA2421078A1 - Procede servant a determiner des alleles - Google Patents

Procede servant a determiner des alleles Download PDF

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Publication number
CA2421078A1
CA2421078A1 CA002421078A CA2421078A CA2421078A1 CA 2421078 A1 CA2421078 A1 CA 2421078A1 CA 002421078 A CA002421078 A CA 002421078A CA 2421078 A CA2421078 A CA 2421078A CA 2421078 A1 CA2421078 A1 CA 2421078A1
Authority
CA
Canada
Prior art keywords
primer
nucleic acid
heterosequence
site
hybridized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002421078A
Other languages
English (en)
Inventor
Xiangjun Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2421078A1 publication Critical patent/CA2421078A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés et des trousses servant à séparer et à identifier des allèles et, par conséquent, l'haplotype, dans des spécimens d'ADN génomique. Ce procédé consiste généralement à effectuer l'hybridation d'amorces spécifiques pour des sites polymorphes à l'intérieur de ces allèles aux spécimens d'ADN, à prolonger ces amorces par un ou plusieurs acides nucléiques, à séparer les amorces prolongées et à identifier les allèles au moyen de l'amorce prolongée. Ce procédé permet également d'effectuer la ligation de deux amorces, leur séparation et leur utilisation ultérieure afin d'identifier l'allèle ciblé. Ce procédé permet, de plus, d'utiliser une autre amorce en tant que site bloquant pour l'extension de la première amorce, de façon à répliquer et à identifier un segment d'ADN comprenant un site polymorphe. On peut marquer les amorces prolongées ou non, de manière à pouvoir séparer et/ou identifier l'amorce sans difficultés.
CA002421078A 2000-08-30 2001-08-30 Procede servant a determiner des alleles Abandoned CA2421078A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22899400P 2000-08-30 2000-08-30
US60/228,994 2000-08-30
PCT/US2001/041956 WO2002018659A2 (fr) 2000-08-30 2001-08-30 Procede servant a determiner des alleles

Publications (1)

Publication Number Publication Date
CA2421078A1 true CA2421078A1 (fr) 2002-03-07

Family

ID=22859388

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002421078A Abandoned CA2421078A1 (fr) 2000-08-30 2001-08-30 Procede servant a determiner des alleles

Country Status (5)

Country Link
JP (1) JP2004520812A (fr)
CN (1) CN1293204C (fr)
AU (1) AU2001289177A1 (fr)
CA (1) CA2421078A1 (fr)
WO (1) WO2002018659A2 (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003035671A2 (fr) * 2001-10-24 2003-05-01 Singulex, Inc. Procedes destines a detecter des haplotypes genetiques par interaction avec des sondes
CA2465868C (fr) 2001-11-13 2017-02-28 The Trustees Of The University Of Pennsylvania Methode de detection et/ou d'identification de sequences de virus associes aux adenovirus (aav) et d'isolation de nouvelles sequences ainsi identifiees
EP3339430A1 (fr) 2001-12-17 2018-06-27 The Trustees of The University of Pennsylvania Séquences de sérotype 9 de virus adéno associé, vecteurs les contenant et leurs utilisations
AU2002360291A1 (en) 2001-12-17 2003-06-30 The Trustees Of The University Of Pennsylvania Adeno-associated virus (aav) serotype 8 sequences
JP3752466B2 (ja) 2002-04-24 2006-03-08 株式会社日立製作所 遺伝子検査方法
EP1536021A1 (fr) 2003-11-27 2005-06-01 Consortium National de Recherche en Genomique (CNRG) Méthode pour le typage de HLA
GB0406863D0 (en) * 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
US20050282195A1 (en) * 2004-04-30 2005-12-22 Applera Corporation Compositions, methods, and kits for (MIS)ligating oligonucleotides
GB0600116D0 (en) * 2006-01-05 2006-02-15 Univ Cardiff Allele-specific sequencing
MX2010001440A (es) * 2007-08-07 2010-05-20 Monsanto Technology Llc Metodos y composiciones para seleccionar plantas de soja resistentes a nematodos de nudos radiculares del sur.
EP3249053A1 (fr) * 2009-03-27 2017-11-29 Life Technologies Corporation Procédés, compositions et kits de détection de variants alléliques
JP2011072222A (ja) * 2009-09-29 2011-04-14 Kitasato Otsuka Biomedical Assay Kenkyusho:Kk 標的核酸の検出方法
ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
CA2828535A1 (fr) * 2011-02-28 2012-09-07 Transgenomic, Inc. Trousse et procede de sequencage d'un adn cible dans une population mixte
WO2012135664A2 (fr) 2011-03-31 2012-10-04 Dana-Farber Cancer Institute, Inc. Procédés et compositions pour permettre une cold-pcr multiplexe
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
WO2015013166A1 (fr) 2013-07-24 2015-01-29 Dana-Farber Cancer Institute, Inc. Procédés et compositions pour permettre l'enrichissement d'allèles d'adn mineurs par limitation du temps de dénaturation en pcr ou permettre simplement l'enrichissement d'allèles d'adn mineurs par limitation du temps de dénaturation en pcr
EP3778627A1 (fr) 2014-03-09 2021-02-17 The Trustees of The University of Pennsylvania Compositions utile dans le traitement de carence de l'otc
CA3046953A1 (fr) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions et procedes pour le codage par code-barres moleculaire de molecules d'adn avant l'enrichissement des mutations et/ou la detection des mutations
WO2019023243A1 (fr) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. Procédés et compositions pour sélectionner et amplifier des cibles d'adn dans un mélange de réaction unique
WO2025049432A1 (fr) * 2023-08-28 2025-03-06 Intellia Therapeutics, Inc. Procédés d'édition de hla-a dans des cellules pré-criblées pour l'absence d'un ou des deux allèles d'hla-h*01
CN118207338B (zh) * 2024-05-20 2024-09-13 杭州迪谱医学检验实验室有限公司 一种基于核酸质谱的多重超敏体细胞突变检测方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU656514B2 (en) * 1989-02-13 1995-02-09 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
US5468611A (en) * 1990-06-27 1995-11-21 The Blood Center Research Foundation, Inc. Method for HLA typing
EP0607151B1 (fr) * 1992-06-17 2002-11-13 City Of Hope Procede de detection de sequences d'acide nucleique et de discrimination entre ces sequences
GB9503808D0 (en) * 1995-02-24 1995-04-12 Univ Nottingham Detection assay
EP2332958B1 (fr) * 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Détection de différences entre séquences d'acide nucléique faisant appel à la réaction de détection par ligase et à des réseaux adressables
WO1998013527A2 (fr) * 1996-09-24 1998-04-02 Rapigene, Inc. Compositions et procedes pour l'augmentation de la specificite d'hybridation

Also Published As

Publication number Publication date
WO2002018659A3 (fr) 2003-07-31
CN1501982A (zh) 2004-06-02
AU2001289177A1 (en) 2002-03-13
CN1293204C (zh) 2007-01-03
JP2004520812A (ja) 2004-07-15
WO2002018659A2 (fr) 2002-03-07

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