CA3135230C - Methods and devices for cleaning bioreactor media - Google Patents

Abstract

Cette divulgation décrit des méthodes de nettoyage de garnissage et de bioréacteur utilise en assainissement des eaux. Les méthodes comprennent par exemple !Injection de fluide dans le bioréacteur, en ciblant par exemple les zones ()Ca la boue est plus densément solidarisée, et en soutirant sélectivement de bioréacteur la boue désolidarisée par rapport au garnissage.

Description

METHODS AND APPARATUS FOR CLEANING UPHOLSTERY
OF A BIOREACTOR
FIELD OF DISCLOSURE
[0001] This disclosure generally targets the field of water treatment. More specifically, it relates to methods of separation and/or withdrawal of sludge accumulated in bioreactors and more particularly on, in and/or around the lining of a bioreactor used in a fixed culture water treatment process.
STATE OF THE ART
Water treatment

[0002] The fixed culture method constitutes a recognized technology allowing the treatment of waste water. The principle of operation of this process is based on the presence, in a ventilated tank or not, of parts of a generally synthetic material (e.g. filling) offering a large surface area for the attachment and growth of microorganisms and on which develops a microbial biofilm. This biofilm is responsible for the biodegradation and transformation of the polluting matter present in the wastewater, whether in organic form or composed of nutrients usually found in wastewater (nitrogen and phosphorus).

[0003] The parts making up the biofilm growth medium may be mobile due to intrinsic characteristics (size, density) of the material used and the energy applied for mixing, such as for the MBBR (moving bed bioreactor), as described in W0199101 1396A1.
They can also be immobile or partially mobile within the bioreactor, in a tank compartment or occupying a fraction or all available space as described in US7306717B2. In the case of stationary or partially moving parts, these are generally arranged in bulk in the tank of the bioreactor, in a configuration said of filling. Two types of non-mobile packing can be found in this type of process, depending on the geometry of the parts: 1) piled up in fawn Date Received/Date Received 2021-10-20 structured or random on top of each other, for cylindrical shapes, spherical or prismatic or 2) grouped or entangled when the parts are made of strips or flexible filaments (flexible packing not mobile), as described in W02003027031A1.

[0004] Regardless of the biological treatment technology used, It is known that there is multiplication of the microorganisms involved in the degradation of pollutants found in wastewater, when these are found in the presence of biodegradable materials. These microorganisms multiply mainly in the form of biofilm in the case of a bioreactor has fixed culture. There is also a retention, within the process, of the material particle found in wastewater. The biofilm and the particles that accumulate within the process is called sludge. When the waste water supply is regular, we can consider that this accumulation of mud is continuous. The cumulative amount of mud found in the process is therefore intrinsically linked to the quantity of material degraded pollutant (cumulative volume of water treated, concentration of pollutants) and over time.

[0005] It is known that the design of treatment systems has fixed culture on non-moving flexible packing is based, among other things, on their ability to retain solid matter in the bioreactor, in order to ensure a effluent quality meeting the local health standards in force for allow it to return to the receiving environment. The sludge produced accumulates therefore in the bioreactor, either at the bottom of the tank by decantation or by accumulation within the flexible packing itself. Despite mineralization partial of this sludge by endogenous respiration of microorganisms and mineralization of biodegradable organic matter, there comes a time 00 the amount of residual sludge in the reactor is sufficient to impede the treatment efficiency of the process. It is known that the mud which accumulates in large quantities in a bioreactor occupy space that is no longer available for water and air flows. It leads to generally Date Received/Date Received 2021-10-20 to a short-circuiting of the water to be treated through the tank, and therefore to a reduction in the retention time of pollutants within the bioreactor, which to translated by a loss of purification performance of the treatment process. A
Excessive sludge build-up also leads to shorting of the air bubbles injected into the tank, when the system or the compartment is ventilated, or even partial or complete blockage of the air diffuser. That disruption of aeration ultimately limits the amount of oxygen available to microorganisms, reducing the effectiveness of the treatment. It also plays on the quality of the mixture in the bioreactor, necessarily reducing the quality the scouring of the packing and the bringing into contact between the microorganisms and pollutants. Finally, the blockage of the diffuser can increase the loss of load in the vent pipe and generate premature breakage of the aeration equipment used, e.g. compressor, blower or the diffuser. All of these events can result in a loss of purifying performance due to the establishment of conditions unfavorable to microbial activity.

[0006] The design of fixed culture treatment systems on flexible non-moving padding, with limited surface coverage of treatment by the air diffusers and the presence of a filling such as a group or tangled filamentous packing occupying almost all of the volume available, affects the quality of the mixture within the bioreactor. That results in uneven scouring of upholstery pieces, and merle has localized accumulation of mud which, over time, eventually becomes compact and solid. Moreover, he is known in the field of decentralized sanitation that the reality of each site is unique in point view of the flow and the quality of the water to be treated, the concentrations of pollutants encountered, the water production system or even the quality installation of treatment system components. This variability Between sites limits the ability to precisely predict how the bioreactor Date Received/Date Received 2021-10-20 will evolve over time and Ca the solid masses of mud will eventually accumulate.
Cleaning method

[0007] It is known that waste water treatment systems based on a fixed culture generally use a system of scouring of the packing pieces, which helps to maintain a biofilm thickness favorable to microbial activity. The sludge generated can remain within the process, but they are preferably removed from the bioreactor and then transferred to a dedicated storage or processing area. Scrubbing is generally obtained by injecting air bubbles at the base of the packing in the case of non-moving parts. In decentralized sanitation, sludge produced is typically stored within the bioreactor itself in the case non-moving flexible packing processes, in order to limit the cost of management sludge (storage tank, separation device, equipment for transfer). Thus, although scrubbing by air injection under the upholstery allows some control of the thickness of the biofilm developing there, it does not does not avoid the accumulation of sludge in the bioreactor.

[0008] At present, there are at least three in the industry methods for removing excess sludge from a bioreactor non-moving flexible packing:
1) Push the filamentous filling towards a wall or the bottom of the tank to then extract the sludge which is found in the fraction of the tank momentarily free of filling. Often the mud subtracted is returned to the septic tank located upstream of the bioreactor in order to achieve a solid-liquid separation for example by decantation. The clarified water then returns to the gravity flow bioreactor. This way of doing does not allow only partial removal of sludge from the bioreactor. When they are Date Received/Date Received 2021-10-20 returned to the septic tank, in addition to keeping them in the treatment chain until the next pit emptying, this creates a hydraulic peak in the pit which is likely to affect its good functioning.
2) Using a pipe positioned at the bottom of the bioreactor, the sludge is drawn off by a hydrocleaner. Typically, the pipes used in this type of operation are those favored by the operators of hydrocleaner, having a diameter of 75 to 100 mm. Experience shows that this operation does not allow the masses of mud to be removed localized which have come together over time, leaving a filling partially obstructed for backflow of water and air. Moreover, experience also shows that the filaments or packing strips tend to be carried away in the sludge draw-off line, interfering with the proper functioning of the sewer cleaner.
3) The packing is completely removed from the bioreactor and scrubbed outside the tank using pressurized water. Residual mud still present in the bioreactor is then withdrawn by hydrocleaning and then be disposed of according to local regulations.
This approach allows sufficient cleaning of the upholstery to restore the purifying function of the system. However, it is expensive because of the time required, it increases the risk of contamination by bioaerosols or simply by direct contact with the material or droplets generated, and is unsightly to the client.

[0009] There is therefore a need for a method which would allow to avoid or to minimize at least one of the disadvantages mentioned above.
DISCLOSURE SUMMARY

[00010] In one aspect, the present disclosure relates to a method separation of sludge accumulated on the surface, in and/or around a packing a bioreactor for the treatment of waste water, the method Date Received/Date Received 2021-10-20 comprising injecting a fluid into the bioreactor so that the power dissipated is sufficient to separate at least part of the sludge accumulated, the power dissipated being approximately 0.5 to 900 Watts.

[00011] According to another aspect, the present disclosure relates to a method for separating sludge accumulated on the surface, in and/or around of a packing of a bioreactor for the treatment of waste water, the method including inject at least one jet of a fluid with sufficient power to loosen at least a portion of the accumulated sludge; and move and/or reorient the jet in order to separate at least one other part of the accumulated mud.

[00012] According to another aspect, the present disclosure relates to a method for separating sludge accumulated on the surface, in and/or around packing placed in a bioreactor for water treatment used, the method comprising select a first zone to be separated in the bioreactor, the first zone comprising an accumulation of mud;
injecting into the first zone a fluid with a power sufficient to loosen at least a portion of the sludge accumulated;
select a second zone to uncouple in the bioreactor, the second zone comprising an accumulation of mud; and injecting into the second zone the fluid with a power sufficient to loosen at least a portion of the sludge accumulated.

[00013] According to another aspect, the present disclosure relates to a method for drawing off sludge accumulated on the surface, in and/or around a Date Received/Date Received 2021-10-20 packing a bioreactor for the treatment of waste water, the method including:
injecting a fluid into the bioreactor has sufficient power to loosen at least part of the accumulated sludge; and selectively withdrawing from said bioreactor the sludge separated by report to said packing.

[00014] According to another aspect, the present disclosure relates to a kit for removing sludge accumulated on the surface, in and/or around a packing of a bioreactor for the treatment of waste water, and/or accumulated in the bioreactor, the kit comprising:
an injection nozzle, fluid injection equipment, a draw-off strainer, a suction duct, and a suction mechanism, the strainer being sized fawn to selectively draw off the sludge separated from said packing.

[00015] According to another aspect, the present disclosure relates to a unit removal of sludge accumulated on the surface, in and/or around the packing in a bioreactor for the treatment of waste water, and/or accumulated in the bioreactor, the unit comprising:
an injection nozzle intended to inject a fluid to separate at least part of the accumulated sludge, fluid injection equipment for communicating fluid with the nozzle, a draw-off strainer, a suction mechanism, and a suction conduit for being in fluid communication with the strainer and the suction mechanism, Date Received/Date Received 2021-10-20 the strainer being sized fawn to selectively draw off the sludge separated from said packing.

[00016] According to another aspect, the present disclosure relates to a nozzle to separate a sludge from a bioreactor, the nozzle comprising a portion anterior and a posterior portion, the posterior portion serving to !Injection of the fluid, the nozzle being dimensioned so as to transmit a power sufficient for separation.

[00017] According to another aspect, the present disclosure relates to a use of the nozzle described in the present application to separate the least part of a sludge accumulated on the surface, in and/or around a packing placed in a bioreactor for the treatment of waste water and/or accumulated in the bioreactor.

[00018] According to another aspect, the present disclosure relates to a strainer for withdrawing sludge from a bioreactor, the strainer comprising one or multiple orifices and is intended to be in fluid communication with a leads suction, the strainer being sized so as to selectively draw a sludge separated from a packing of said bioreactor.

[00019] According to another aspect, the present disclosure relates to a use of the strainer described in the present application to extract selectively separated sludge with respect to available packing in a bioreactor for wastewater treatment.

[00020] According to another aspect, the present disclosure relates to a method for cleaning a bioreactor and/or a packing thereof including:
the implementation of the sludge separation method such as defined in this application; and the withdrawal of the separated sludge.

Date Received/Date Received 2021-10-20

[00021] According to another aspect, the present disclosure relates to a method for cleaning a bioreactor and/or a packing thereof including:
the separation of at least part of an accumulated sludge in the bioreactor and/or on the surface, in and/or around a packing said bioreactor; and the implementation of the sludge extraction method such as defined in this application.
BRIEF DESCRIPTION OF FIGURES

[00022] The figures of the present disclosure illustrate in one way no-!imitative various examples.

[00023] FIG. 1A shows a perspective view of a nozzle injection according to an example of the present disclosure;

[00024] FIG. 1B shows a side view of the injection nozzle of the Fig. 1A;

[00025] FIG. 1C shows a front view of the injection nozzle of the Fig. 1A;

[00026] FIG. 2A shows a perspective view of a nozzle injection according to another example of the present disclosure;

[00027] FIG. 2B shows a side view of the injection nozzle according to the Fig. 2A;

[00028] FIG. 20 shows a front view of the injection nozzle according to Fig. 2A;

[00029] FIG. 3A shows a perspective view of a nozzle injection according to another example of the present disclosure;

[00030] FIG. 3B shows a side view of the injection nozzle according to the Fig. 3A;

Date Received/Date Received 2021-10-20

[00031] FIG. 30 shows a front view of the injection nozzle according to Fig. 3A;

[00032] FIG. 4A shows a perspective view of a nozzle injection according to another example of the present disclosure;

[00033] FIG. 4B shows a side view of the injection nozzle according to the Fig. 4A;

[00034] FIG. 40 shows a front view of the injection nozzle of air according to Fig. 4A;

[00035] FIG. 5A shows a perspective view of a strainer of withdrawal connected to a suction conduit according to an example of the present disclosure;

[00036] FIG. 5B shows a closer view of the strainer of racking according to Fig. 5A;

[00037] FIG. 5C shows a side view of the draw-off strainer connected to the suction pipe according to Fig. 5A, the suction duct having variable length;

[00038] FIG. 5D shows a front view of the racking strainer according to Fig. 5A;

[00039] FIG. 6A shows a perspective view of a strainer of withdrawal according to another example of the present disclosure;

[00040] FIG. 6B shows a side view of the draw-off strainer according to Fig. 6A;

[00041] FIG. 6C shows a front view of the draw-off strainer according to Fig. 6A;

[00042] FIG. 6D shows a top view of the sump strainer according to Fig. 6A;

Date Received/Date Received 2021-10-20

[00043] FIG. 7A shows a perspective view of a strainer of withdrawal according to another example of the present disclosure;

[00044] FIG. 7B shows a side view of the draw-off strainer according to Fig. 7A;

[00045] FIG. 7C shows a front view of the draw-off strainer according to Fig. 7A;

[00046] FIG. 7D shows a top view of the drawdown strainer according to Fig. 7A;

[00047] FIG. 8A shows a perspective view of a strainer of withdrawal according to another example of the present disclosure;

[00048] FIG. 8B shows a side view of the draw-off strainer according to Fig. 8A;

[00049] FIG. 8C shows a front view of the draw-off strainer according to Fig. 8A;

[00050] FIG. 8D shows a top view of the sump strainer according to Fig. 8A;

[00051] FIG. 9 is a diagram illustrating a cleaning method of one bioreactor and/or a packing thereof comprising a separation and a sludge withdrawal according to an example of the present disclosure;

[00052] FIG. 10 is a diagram illustrating a cleaning method of a bioreactor and/or a packing thereof comprising a desolidarization and sludge withdrawal according to another example of the present disclosure;

[00053] FIG. 11 is a diagram illustrating a cleaning method of a bioreactor and/or a packing thereof comprising a separation and sludge withdrawal according to yet another example of the this disclosure;

Date Received/Date Received 2021-10-20

[00054] FIG. 12 is a diagram illustrating a cleaning method of a bioreactor and/or a packing thereof comprising a desolidarization and sludge withdrawal according to another example of the present disclosure;

[00055] FIG. 13 is a diagram illustrating a cleaning method of a bioreactor and/or a packing thereof comprising a desolidarization and a sludge withdrawal according also to another example of this disclosure;

[00056] FIG. 14 is a diagram illustrating a method of uncoupling according to an example of the present disclosure;

[00057] FIG. 15 is a diagram illustrating a method of sludge decoupling according to another example of the present disclosure; and

[00058] FIG. 16 is a diagram illustrating a withdrawal method of mud according to an example of the present disclosure.

[00059] For a better understanding of the different modes of achievement described here and to demonstrate more clearly how these different embodiments can be made, the reference will be done, by way of example, to the appended drawings, which represent at least one example of embodiment.
DETAILED DESCRIPTION OF THIS DISCLOSURE

[00060] Several embodiments are described herein request, and are for illustrative purposes only. The modes of achievement described are not intended to be limiting in any way. The this disclosure is applicable to many embodiments, as is evident in the disclosure described below. The skilled person acknowledge that this disclosure may be practiced with modifications and changes without departing from the teachings disclose. Although special features of this Date Received/Date Received 2021-10-20 disclosure can be described by referring to one or more modes of realization or particular illustrations, it must be understood that these features are not limited to use in one or more particular embodiments or illustrations referring to which they are described.

[00061] The term non-moving packing )> used herein request refers to corn parts providing a biofilm growth support which are immobile or partially mobile within a bioreactor. The large quantity of parts ending up in the bioreactor, added to their intrinsic characteristics (geometry, density of the material, entanglement), hinder their free movement in the bioreactor. That hinders imposes either immobility on them or only allows them to move low amplitude in a localized field, hence the notion of partially mobile. Due to the low amplitude of movement of the parts of partially mobile packing, those skilled in the art generally consider them as immobile. The term non-mobile is used without preference and inclusive way to talk about stationary trim pieces, partially mobiles or both at the same time. Non-moving packing can be classified according to the geometry of the pieces: 1) stacked in structured or random fawn on top of each other, for cylindrical, spherical or prismatic or 2) grouped or entangled when the pieces are formed strips or flexible filaments.

[00062] The term fluid used in the present application means a gas or liquid.

[00063] The term equivalent diameter used herein demand corresponds to the diameter of a circle of area equivalent to that of the orifice of non-circular shape, for example of geometric shape, a polygon or for example an irregular or atypical shape.

[00064] The term around the packing used herein request refers to any sludge accumulating on the periphery of the packing, Date Received/Date Received 2021-10-20 bottom of the bioreactor, on one or more walls of the bioreactor, and/or at the surface of the liquid contained in the bioreactor.

[00065] The terms a mode of realization, mode, modes of embodiment , embodiment , embodiments , a Where several embodiments, and some embodiments want mean one or more (but not all) embodiments of the present disclosure, unless expressly stated otherwise.

[00066] The terms including, comprising and variants thereof this mean including, but not limited to, unless expressly stipulates other. A list of items does not mean that any or all the elements are mutually exclusive, unless expressly stated other. The terms un/une and le/la mean one or more , except if expressly states otherwise.

[00067] Furthermore, although the steps of a process can be described (in the disclosure and/or in the claims) in sequential order, such processes can be configured to work in an order alternative. In addition, any sequence or order of steps that can be described does not necessarily indicate a requirement that measurements be made in this order. The process steps described here can be performed in any order that is convenient. In addition, some steps may be carried out simultaneously.

[00068] When a single device or object is described here, it will be obvious that more than one device/object (whether cooperating or not) can be used at the same time instead of one single device/object. Similarly, when more than one device or object is described in this (whether they cooperate or not), it will be obvious that only one device/object can to be=
uses instead of more than one device or object.

[00069] It should be noted that degree terms such as substantially, about and approximately when they are used herein means a reasonable amount of deviation from the term Date Received/Date Received 2021-10-20 modified, so that the final result is not significantly amended. These degree terms should be interpreted as including a deviation of the modified term if this deviation did not contradict the meaning of term that it modifies.

[00070] In addition, the recitation of numerical ranges by dots of extremity in this includes all the numbers and fractions encompassed in this range (for example, 1 to 5 includes 1, 1.5, 2, 2.75, 3, 3,90,4 and 5). II
is also understood that all numbers and fractions thereof are supposed to be modified by the term environ which signifies a variation up to a certain amount of the number to which reference is made if the result final does not change significantly.

[00071] Further, the expression and/or >> as used herein, means a or >> inclusive. In other words, <X and/or Y >>, for example, means X or Y
or both, and X, Y and/or Z >> means X or Y or Z or whatever what possible combination of these.

[00072] It is demonstrated in the present disclosure that a selective separation of sludge present within the packing, directly in the bioreactor, using a fluid injection nozzle and the withdrawal of this sludge separated with the help of a suitable extraction strainer allows to optimize the quantity of sludge extracted from the bioreactor.

[00073] Thus, the present application includes a method of cleaning or separation of sludge accumulated on the surface, in and/or around a packing of a bioreactor for the treatment of waste water. The method includes: injecting at least one jet of fluid with sufficient power to loosen at least a portion of the accumulated sludge; and move and/or redirect the jet in order to separate at least another part of the sludge accumulated. This method of uncoupling 100 is illustrated in FIG.
9.More particularly, the power dissipated being about 0.5 to 900 Watts.

[00074] For example, the power dissipated is approximately 0.5 at 600 watts.

Date Received/Date Received 2021-10-20

[00075] For example, the power dissipated is approximately 3 to 400 watts.

[00076] For example, the power dissipated is approximately 3 to 200 watts.

[00077] For example, the fluid is injected at a rate of about 5 L/sec a about 50 L/sec.

[00078] For example, the fluid is injected at a rate of about 15 L/sec a about 35 L/sec.

[00079] For example, the fluid is injected for approximately 1 to approximately minutes.

[00080] For example, the fluid is injected for approximately 3 to approximately minutes.

[00081] For example, the fluid is injected for about 5 to about minutes.

[00082] For example, the fluid is injected through a nozzle.

[00083] For example, the nozzle comprises a front portion and a posterior portion.

[00084] For example, the nozzle has an equivalent diameter of about 5 mm to about 127mm.

[00085] For example, the nozzle has an equivalent diameter of about 10 mm about 100mm.

[00086] For example, the nozzle has an equivalent diameter of about 20 mm about 80mm.

[00087] For example, the nozzle has an equivalent diameter of about 20 mm about 60mm.

[00088] For example, the nozzle has an equivalent diameter of about 20 mm about 30mm.

Date Received/Date Received 2021-10-20

[00089] For example, the nozzle is fitted with a cap.

[00090] For example, the nozzle cap is flat, dome-shaped, tapered or bevelled.

[00091] For example, the nozzle cap comprises one or more orifices.

[00092] For example, the rear portion of the nozzle comprises one or several holes.

[00093] For example, each orifice has an equivalent diameter of approximately mm is about 127 mm.

[00094] For example, each orifice has an equivalent diameter of approximately mm is about 100 mm.

[00095] For example, each orifice has an equivalent diameter of approximately mm is about 80 mm.

[00096] For example, each orifice has an equivalent diameter of approximately mm is about 50 mm.

[00097] For example, each orifice has an equivalent diameter of approximately 20mm to about 30mm.

[00098] For example, the fluid is injected according to an orientation longitudinal, lateral and/or diagonal relative to the nozzle.

[00099] For example, the nozzle is a static nozzle or a mobile nozzle.

[000100] For example, the static nozzle is arranged on a wall of a tank of said bioreactor or in a bottom of said tank.

[000101] For example, the movable nozzle is a motorized nozzle or a nozzle manipulated by an articulated arm or a user.

[000102] For example, the movable nozzle is moved inside said bioreactor.

Date Received/Date Received 2021-10-20

[000103] For example, the front end of the nozzle is connected to air injection equipment.

[000104] For example, the front end of the nozzle is connected to compressor or blower.

[000105] For example, the fluid is a gas.

[000106] For example, the gas comprises air.

[000107] For example, the fluid is a liquid.

[000108] For example, the fluid is a recirculated liquid.

[000109] For example, the liquid comprises water.

[000110] For example, the method further comprises a step of withdrawing from said bioreactor at least part of the desolidarized sludge.

[000111] For example, the method further comprises an aeration step from the bottom of a tank of said bioreactor.

[000112] For example, the aeration takes place before the injection of fluid.

[000113] For example, the aeration takes place before the injection of fluid and during fluid injection.

[000114] For example, the aeration takes place before the injection of fluid, during fluid injection and during withdrawal.

[000115] For example, aeration takes place during fluid injection and during racking.

[000116] For example, aeration takes place during racking.

[000117] The present application also includes a method of separation of sludge accumulated on the surface, in and/or around a packing of a bioreactor for the treatment of waste water. This method, also depicted in Fig. 14, includes: injecting at least one stream of a fluid Date Received/Date Received 2021-10-20 with sufficient power to separate at least part of the accumulated mud; and move and/or reorient the jet in order to separate the minus another part of the accumulated mud.

[000118] The present application further includes cleaning methods a bioreactor and/or a packing thereof comprising a desolidarization and sludge extraction. Sludge separation is carried out in order to remove the mud accumulated on the surface, in and/or around of one packing placed in a bioreactor for the treatment of waste water. Of such methods are described in Figs. 9, 10, 11, 12 and 13. Methods specific methods of desolidarization of the mud are illustrated in the diagrams of the Figs. 14 and 15. Refer more particularly to FIG. 15, the method includes: selecting a first zone to be separated in the bioreactor, the first zone comprising an accumulation of mud; inject into the first zone a fluid with sufficient power to separate the less part of the accumulated mud; select a second zone a separate in the bioreactor, the second zone comprising a mud accumulation; and injecting into the second zone the fluid with a sufficient power to loosen at least part of the mud accumulated. Fig. 16 shows the diagram of a mud racking method.

[000119] The present application further includes a withdrawal method mud accumulated on the surface, in and/or around a lining of a bioreactor for wastewater treatment. The method includes: injecting a fluid in the bioreactor has sufficient power to separate the less part of the accumulated mud; and selectively withdrawing from said bioreactor the sludge separated from said packing.

[000120] For example, the method includes selecting a plurality of zones to be separated, each zone among the plurality of zones comprising a accumulation of mud.

Date Received/Date Received 2021-10-20

[000121] For example, the selection is made by a user, a computer or artificial intelligence device.

[000122] For example, the nozzle is a motorized nozzle or a nozzle manipulated by an articulated arm or a user.

[000123] For example, the nozzle is moved and/or oriented by a device mechanical, hydraulic, or electronic device, or by a user.

[000124] For example, the sludge is further separated by contacting the mud with the nozzle.

[000125] For example, the sludge is further separated by contacting the mud with the nozzle by moving back and forth with the nozzle.

[000126] For example, the nozzle is moved and/or oriented between the first zone and the second zone.

[000127] For example, the nozzle is moved and/or oriented in such a way simultaneous with the injection into the first zone and/or the second zone.

[000128] For example, following the injection of said at least part of the accumulated sludge or following injection in the first zone, the nozzle is moved and/or oriented and then injected said at least another part of the accumulated sludge or the second zone.

[000129] For example, the nozzle is moved and/or oriented by a user or a mechanical device on a plurality of zones to dissociate.

[000130] For example, the nozzle is moved and/or oriented from fawn to contact with mud accumulated on the surface, in and/or around said packing disposes in the bioreactor.

[000131] For example, the method further comprises a step of withdrawing the desolidarized sludge from said bioreactor.

Date Received/Date Received 2021-10-20

[000132] For example, the fluid is injected inside the bioreactor in order to separate the sludge from said packing while maintaining the sludge in the reactor, then the sludge is withdrawn from said reactor

[000133] For example, the method further comprises an aeration step from the bottom of a tank of said bioreactor.

[000134] For example, the aeration takes place before the injection of fluid.

[000135] For example, the aeration takes place before the injection of fluid and during fluid injection.

[000136] For example, the aeration takes place before the injection of fluid, during fluid injection and during withdrawal.

[000137] For example, aeration takes place during fluid injection and during racking.

[000138] For example, aeration takes place during racking.

[000139] For example, the nozzle is moved and/or oriented towards one or several areas with accumulated mud.

[000140] For example, the nozzle is moved and/or oriented towards one or several areas comprising mud which is accumulated there in a manner simultaneous with the injection.

[000141] For example, the nozzle is moved and/or oriented so as to contact accumulated mud on the surface, in and/or around the lining placed in the bioreactor.

[000142] For example, the front end of the nozzle is connected to fluid injection equipment.

[000143] For example, the front end of the nozzle is connected to compressor or blower.

Date Received/Date Received 2021-10-20

[000144] For example, the nozzle is moved and/or oriented from fawn to contact accumulated mud on the surface, in and/or around the lining placed in the bioreactor.

[000145] For example, the front end of the nozzle is connected to fluid injection equipment.

[000146] For example, fluid injection equipment is a compressor, blower or pump.

[000147] For example, the injection of fluid and the extraction of mud have location simultaneously.

[000148] For example, the withdrawal is carried out by means of a strainer.

[000149] For example, the strainer comprises one or more orifices.

[000150] For example, the strainer comprises 5 to 100 orifices.

[000151] For example, the strainer comprises 10 to 75 orifices.

[000152] For example, the strainer comprises 5 to 50 orifices.

[000153] For example, the strainer comprises 50 to 100 orifices.

[000154] For example, the orifice or said orifices has/have a diameter equivalent of about 2 mm to about 100 mm.

[000155] For example, the orifice or said orifices has/have a diameter equivalent of about 7 mm to about 75 mm.

[000156] For example, the orifice or said orifices has/have a diameter equivalent of about 10 mm to about 60 mm.

[000157] For example, the orifice or said orifices has/have a diameter equivalent of about 12 mm to about 40 mm.

[000158] For example, the strainer has a bevelled shape.

Date Received/Date Received 2021-10-20

[000159] For example, the strainer is connected to a mechanism suction.

[000160] For example, the strainer is connected to a mechanism suction via a suction duct.

[000161] For example, the suction mechanism is a pump or a sewer truck.

[000162] For example, withdrawal takes place at a withdrawal rate of about 300 L/min to 1500 L/min.

[000163] For example, withdrawal takes place at a withdrawal rate of about 400 L/min to 1400 L/min.

[000164] For example, withdrawal takes place at a withdrawal rate of about 475 L/min and 1215 L/min.

[000165] For example, the withdrawal takes place at a suction speed from about 8 L/sec to about 20 L/sec.

[000166] For example, the withdrawal takes place at a suction speed to the orifices of approximately 1.2 m/sec to 2.5 m/sec.

[000167] For example, the packing is of the non-moving type.

[000168] For example, the packing is of the non-moving flexible type.

[000169] For example, a non-moving hose is a filamentous packing bunch or tangle.

[000170] Kits and units for removing sludge accumulated at the surface, in and/or around a packing of a bioreactor for water treatment used, and/or accumulated in the bioreactor, as well as their use, are also included in this application.

[000171] For example, the kit or unit further comprises a device to aerating the bottom of a tank of said bioreactor. Kit and unit include Between Date Received/Date Received 2021-10-20 others a nozzle as described in the present application, as well as a racking strainer as described in this application.

[000172] For example, fluid injection equipment is a compressor, blower or pump.

[000173] Referring now to Figs. 1A, 1B and 1C, the injection nozzle can be connected at its front portion 11 to a mechanical device pushing air towards its posterior portion 13. This mechanical device can be a compressor or a blower or a pump. The posterior portion 13 includes an orifice 14. For example, the rear portion 13 can include one or more orifices whose equivalent diameter of each orifice is for example between 1 and 127 mm. These holes allow the air to be expelled from the nozzle 10 towards the bioreactor. The use of many orifices makes it possible to split the air flow and thus to widen the field application of the air injected. Referring now to Figs. 2A, 2B and 2C, the nozzle injection 20 comprises a front portion 21 whose end 22 can to be=
connected to a mechanical device. The extremity 24 of the posterior portion 23 is provided with a cap 25. The cap 25 may comprise one or more orifices 26 whose equivalent diameter of each orifice is between 1 and 127 mm and whose orientation can be longitudinal, lateral, diagonal or everything arrangement of these orientations making it possible to optimize the action of the ejected air of the nozzle. For example, cap 25 may be flat, domed or conical or beveled in shape to facilitate the penetration of the nozzle injection into the mass of flexible packing. The equivalent diameter of the injection nozzle is, for example, less than 127 mm in order to facilitate its manipulation by the operator and his penetration into the packing mass flexible.

[000174] Figs. 3A, 3B and 3C illustrate a nozzle 30 which includes a front portion 31 whose end 32 can be connected to a device mechanism pushing a fluid towards its posterior portion 33. The end 34 of the rear portion 33 is provided with a plug 35. The rear portion 33 Date Received/Date Received 2021-10-20 comprises a plurality of orifices 36 of 6.35 mm in diameter each. Figs.
4A, 4B and 4C illustrate a nozzle similar to that in Figs. 3A, 3B and 3C, however the nozzle 40 comprises at the rear portion 43 a plurality of orifices 46 of smaller diameter, ie 2 mm.

[000175] The nozzle is maneuvered by the operator so as to inject the air has a flow rate between 300 L/min (5 L/sec) and 3000 L/min (50 L/sec), through example between 1000 L/min (17 L/sec) and 2000 L/min (35 L/sec) in the filling. When air is injected into water, the power dissipated (P) by the rising air bubbles in the liquid can be estimated by equation P = paValn(pc/pa), 00 pa corresponds to atmospheric pressure, Va to volume of air at atmospheric pressure and pc at the pressure at the injection point (as that described in Metcalf & Eddy Inc., Tchobanoglous, G., Burton, FL, Tsuchihashi, R., & Stensel, HD (2013). Wastewater engineering: Treatment and resource recovery (5th ed.). McGraw-Hill Professional). In the pitch spectrum effective liquid loads typically observed in packed bioreactors non-moving hose, for example less than 2 m, we find for these flow rates of air injected between the surface of the water and the bottom of the bioreactor, a Powerful power dissipation varying between 0.5 W and 900 W. A power dissipation of 0.5 W
corresponds to an injection of air 1 cm below the surface of the liquid, at a flow rate of 300 L/min. A dissipated power of 900 Watts corresponds to an injection of air at a flow rate of 3000 L/min at 2 meters water depth. We will aim to preferably an air flow generating a dissipated power between 3 and 200 W, in order to ensure the efficiency of the operation while limiting the risks for health human. The rear portion of the nozzle is moved and/or pushed to through the packing in order to reach the solid masses of mud, which it that is on the surface of the water, at the bottom of the tank, in its recesses or at a height intermediate. The entire bioreactor volume can be covered by this operation, but for example an emphasis could be applied to regions 00 the mud is more cohesive and supportive. Depending on the cohesion and level of solidarity of the masses of mud, the operator can let the air bubbles outgoing Date Received/Date Received 2021-10-20 from the rear portion of the nozzle, act for a few seconds (for example 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 or 110 seconds) for separate the mud from a specific area and/or perform movements of ve and comes with the nozzle to help loosen the mud and increase the field of influence of air bubbles. This operation can be extend a few minutes (for example, 2, 3, 4, 5, 6, 7, 8, 9 or 10 minutes) by field of application, when the mass of mud is important and very cohesive. The whole of this operation can last from 1 to 30 minutes.

[000176] Referring now to Figs. 5 to 8, different modes of construction of a strainer are described. Referring to Figs. 5A, 5B, 50 and 5D, the extraction strainer 50 is connected to a suction line 51, itself even if connected to a mechanical suction device allowing the sludge separated from the bioreactor. This mechanical suction device can be a pump, or for example a sewer truck to facilitate the subsequent management of the extracted sludge. The posterior portion 52 of the strainer may be composed of a bevelled end 53 facilitating its insertion into flexible packing. The bevelled end may include one or more orifices 54 whose equivalent diameter is for example greater than 2 mm, in order to allow the passage of mud particles, and for example equal or less than 100 mm in order to avoid the flexible packing being carried away by the suction unit. The strainers of Figs. 6, 7 and 8 are similar to that of Fig. 5, with the exception of the number of holes and their diameter.

[000177] The strainer of Figs. 5A, 5B, 5C and 5D includes 72 holes of 12.5 mm in diameter each, the strainer of Figs. 6A, 6B, 6C and 6D includes 12 holes of 39 mm in diameter each, the strainer of Figs. 7A, 7B, 7C and 7D
comprises 27 holes of 24 mm in diameter each and the strainer of Figs. 8A, 8B, 8C and 8D includes 3 holes of 84 mm diameter each.

[000178] For example, the draw-off strainer is inserted into the packing using the beveled end to facilitate its passage to through the mass of material. Once the strainer is inserted, the perforated part of it this Date Received/Date Received 2021-10-20 can be oriented in all directions, but will for example be directed down in order to optimize the volume of liquid extracted from the bioreactor. Without be limited to this, a way to facilitate the insertion of the strainer in the flexible packing mass consists of making it run along the inner wall of tank, between the lining and the wall. Once positioned, the suction of sludge is launched until it is partially or completely emptied from the bioreactor.

[000179] The separation of mud with the nozzle can be carried out by at the same time as the sludge withdrawal, partially or completely, simultaneously or alternately. For example, the uncoupling will be completely carried out before starting the sludge extraction in order to to optimize the quantity of sludge that will be extracted during the operation. Through example, the separation of mud with the nozzle will be spread over at least 1 minute before starting sludge removal. When the separation of sludge is carried out in alternating mode, the nozzle can be used as a strainer racking.

[000180] In order to help loosen the sludge, air can be grow at the bottom of the bioreactor using a mechanical aeration device, such as a compressor or blower. A pipeline or a network of pipelines interconnected, connected to the mechanical ventilation device and inserted under the flexible lining allows air to be injected and mud to be scoured presents on the surface pieces of upholstery. This air also allows loosen the mud in areas Ca the mud is less compact. That step can be carried out at the same time as the separation with the nozzle injection of air, partially or completely, simultaneously or in alternates. Likewise, this operation can also be carried out at the same time, partially or completely, simultaneously or alternately with the mud racking. For example, this operation will be performed first, before the separation of sludge with the air injection nozzle, in order to start the separation of the Ca zones the mud is less compact, then will be repeated Date Received/Date Received 2021-10-20 at the same time as the sludge extraction. Those skilled in the art will understand that the addition of this operation allows to increase the zone of influence of the cleaning and to accelerate the separation of mud present in the flexible lining.
Of more, he will understand that this operation carried out at the same time as the racking keeps the sludge in suspension in the liquid phase, to avoid its settling in the tank and thus to optimize the quantity of mud extracted during racking.

[000181] According to one embodiment, the method of cleaning the non-moving flexible packing comprises a first stage of separation of sludge allowing loose sludge to be detached and loosely attached to the packing. This step is performed by injecting the air under the packing using pipes inserted under it, for example, for at least 2 minutes to release a minimum of sludge from the packing.
For example, the air injection will last at least 5 minutes. air flow applied will for example be greater than 5 L/second (L/sec). For example, it can be greater than 15 L/sec.

[000182] This step is followed or carried out simultaneously with a desolidarization of solid sludge masses using a nozzle injection of fluid, for example air, which the operator can move. This last will be able therefore put emphasis on the areas where the mud is more cohesive and solidarity. This nozzle will be connected by its front portion to the pipe flexible or rigid of a mechanical device blowing air. The part posterior comprises one or more orifices whose equivalent diameter of each hole is between 1 and 127 mm. These holes allow air to be expelled from the nozzle towards the bioreactor. The use of several orifices makes it possible to split the air flow and thus to widen the field of application of air injects. The rear end can be fitted with a plug. the cork may include one or more orifices of equivalent diameter greater than 1 mm, for example 25 mm in diameter to increase the efficiency of the cleaning of the packing by the rise of larger air bubbles. The flow Date Received/Date Received 2021-10-20 of air applied will be at least 5 L/sec to ensure good dissociation of sludge, for example greater than 15 L/sec. The duration of this operation will be more or less long depending on the level of mud cohesion and its quantity, but should typically take between 5 and 20 minutes for the entire bioreactor.

[000183] The last stage consists in drawing off the unsolidified sludge from using a strainer, for example a beveled strainer, having one or more orifices whose equivalent diameter is for example greater than 2 mm, by example composed of several orifices with a diameter equivalent to approximately 12 mm. The strainer is for example connected to the suction line of a water cleaner. For example, the strainer holes will face down low in order to optimize the volume of liquid extracted. This step can be done of concomitant manner with an injection of air under the packing using pipes inserted under it. This will keep in suspension the separated sludge and thus to optimize the extraction. air flow applied will for example be greater than 5 L/second (L/sec), or even greater than 15 L/sec.

[000184] It is thus demonstrated in the present disclosure a racking of quick and inexpensive mud that allows selective fawn separation of areas of the Ca bioreactor the sludge has mainly accumulated, to optimize the quantity of sludge removed, to prevent or reduce the washout of the packing during sludge extraction, and/or to protect the health of the operator by limiting contact with contaminated equipment, droplets and bioaerosols.
Example 1: Sludge tapping

[000185] Tests have been carried out in order to evaluate the performance of sludge draw-off according to different approaches compared to a draw-off simple. Simple racking is the most common method of sludge extraction.

simple during which a hydrocleaner pumps the liquid phase of the bioreactor, by inserting the suction line at the bottom of the tank, without having Date Received/Date Received 2021-10-20 previously neither intentionally shakes nor cleans the upholstery. Mud extracted was quantified using suspended solids (SS) as reference parameter.

[000186]
bioreactors with non-moving flexible packing, in operation for more than 7 years, were treated according to one of the five scenarios following. All bioreactors were compartmentalized in 2 sections hydraulically interconnected.
1) Desolidarization followed by withdrawal: As described in FIG. 10, one decoupling of the sludge 100 is carried out using the nozzle air injection for 0.75 to 1.5 min per compartment, fitted with a single-perforated cap with a 25 mm orientation diameter hole longitudinal, as illustrated in Figs. 1A, 1B, and 1C. The flow of air injection was between 17 and 35 L/sec. Mud racking 110 was then carried out using a hydrocureur and a strainer bevelled multi-perforated with 72 holes of 12.5 mm in diameter, as as illustrated in Figs. 5A, 5B, 50 and 5D.
2) Prolonged separation followed by racking: As described in Fig. 11, a prolonged separation of the sludge 200 is carried out by using the air injection nozzle for 4.5 to 8.75 min per compartment, fitted with a single-perforated plug with a 25 mm hole of longitudinal orientation diameter, as illustrated in Figs. 1A, 1B
and 1C. The air injection rate was between 17 and 35 L/sec. the extraction of mud 210 was then carried out using a hydrocleaner and a multi-perforated beveled strainer with 72 holes of 12.5 mm in diameter, as shown in Figs. 5A, 5B, 5C and 5D.
3) Aeration of the bottom, followed by racking in the presence of aeration of the background: As described in Fig. 12, air is injected 320 at the bottom of the bioreactor for 4 to 6 min, under the packing, by two pipes of 50 mm arranged under the packing, at a flow rate of 17 L/sec. the Date Received/Date Received 2021-10-20 300 sludge extraction was then carried out using a hydrocleaner and a multi-perforated beveled strainer with 72 holes of 12.5 mm in diameter, as shown in Figs. 5A, 5B, 50 and 5D. The ventilation at the bottom 320 was maintained during the withdrawal of desolidarized sludge 310.
4) Aeration of the bottom, followed by separation and then racking:
As described in FIG. 12, air is injected into the bottom of the bioreactor 400 for 3.25 to 4.25 min, under the packing, by two pipes of 50 mm arranged under the packing, at a flow rate of 17 L/sec, followed by a separation 410 using the air injection nozzle for 5 minutes per compartment of the bioreactor, fitted with a shaped plug conical multi-perforated 12 holes of 7 mm in diameter, orientation lateral and diagonal, as illustrated in Figs. 2A, 2B and 20. The flow of air injection was between 17 and 35 L/sec. Mud racking 420 was then carried out using a hydrocleaner with a strainer bevelled multi-perforated with 72 holes of 12.5 mm in diameter, as as illustrated in Figs. 5A, 5B, 5C and 5D.
5) Aeration of the bottom, followed by desolidarization and then racking in the presence of bottom aeration: As described in FIG. 13, air is injects 500 at the bottom of the bioreactor for 4 to 6 min, under the packing, by two 50 mm pipes arranged under the packing, at a flow rate of 17 L/sec, followed by a 510 decoupling using the nozzle air injection for 5 minutes per compartment of the bioreactor, fitted with a multi-perforated conical plug with 12 holes of 7 mm in diameter, lateral and diagonal orientation, as illustrated in Figs. 2A, 2B and 2C. The air injection rate was between 17 and 35 L/sec. The extraction of mud 520 was then carried out using a sewer with a multi-perforated beveled strainer with 72 holes of 12.5 mm in diameter, as illustrated in Figs. 5A, 5B, 5C and 5D.
Bottom aeration 530 was maintained during sludge drawdown dissociated 520.

Date Received/Date Received 2021-10-20

[000187] The performance results for the amount of sludge extracted of a fixed film bioreactor using non-moving flexible packing are described in Table 1.
Table 1.
Relative gain by Scenario tests report to a simple racking 1) Desolidarization followed by racking 77%
2) Prolonged separation followed by 81% racking 3) Aeration of the bottom, followed by racking 34%
presence of bottom ventilation 4) Aeration of the bottom, followed by separation and 45%
then racking 5) Aeration of the bottom, followed by separation and then racking in the presence of aeration of 228%
background

[000188] The range of power values dissipated by the injected air in bioreactors, under the different conditions tested, is described in Table 2. They have ranged between 3 and 200 W, as shown in Table 2.
Table 2.
Height Pressure Power Air flow injection injection dissipated (L/min) (L/sec) (m) (kPa) (W) 1000 17 1.27 114 196 2000 33 0.01 101 3.3 Example 2: Withdrawal strainers

[000189] Seven variations of racking strainer were tested in order to to assess their ability to prevent the flexible lining from being carried away towards the operation of the sewer cleaner during racking. These variations focused on the number of orifices and their diameter, with a spectrum of diameters ranging from 7 mm to 84 mm and a quantity spectrum ranging from 3 to 147 orifices. More Date Received/Date Received 2021-10-20 particularly, the strainer of Figs. 5A, 5B, 5C and 5D includes 72 holes of 12.5 mm in diameter each, the strainer of Figs. 6A, 6B, 6C and 6D includes 12 holes of 39 mm in diameter each, the strainer of Figs. 7A, 7B, 7C and 7D
comprises 27 holes of 24 mm in diameter each and the strainer of Figs. 8A, 8B, 8C and 8D includes 3 holes of 84 mm diameter each. Moreover, the following three other strainers were evaluated: a strainer comprising 147 holes of 7.3 mm in diameter each, a strainer comprising 108 orifices of 9.4 mm in diameter each and a strainer comprising 48 orifices 16.9 mm in diameter each,

[000190] Under all the test conditions carried out, at flow rates of withdrawal between 475 and 1215 L/min correspond to suction speeds at the orifices between 1.2 and 2.5 m/sec, no carryover of the flexible packing could not be observed.

[000191] The description should be interpreted as an illustration of the present technology, but should not be considered as limiting the claims. The claims must not be limited in their scope examples, but should be given the widest possible !interpretation consistent with the description as a whole.

Date Received/Date Received 2021-10-20

Claims (135)

CLAIMS: 1. Method for removing sludge accumulated on the surface, in and/or around of one packing a bioreactor for wastewater treatment, the method including:
injecting a fluid into said bioreactor at a power sufficient to separating at least a portion of said accumulated sludge; and selectively withdrawing from said bioreactor the sludge separated from said packing. 2. The method according to claim 1, wherein said fluid is injected for that the power dissipated is from about 0.5 to about 900 Watts. 3. The method according to claim 1, wherein said power dissipated is from about 0.5 to about 600 Watts. 4. The method according to claim 1, wherein said power dissipated is from about 3 to about 400 Watts. 5. The method according to claim 1, wherein said power dissipated is from about 3 to about 200 W. 6. The method according to any one of claims 1 to 5, in which said Fluid is injected at a rate of about 5 L/sec to about 50 L/sec. 7. The method according to any one of claims 1 to 5, in which said Fluid is injected at a rate of approximately 15 L/sec to approximately 35 L/sec. 8. The method according to any one of claims 1 to 7, in which said fluid is injected into said bioreactor for about 1 minute at about 30 minutes. 9. The method according to any one of claims 1 to 7, in which said fluid is injected into said bioreactor for about 3 minutes at about 25 minutes.

Date Received/Date Received 2022-04-29 10. The method according to any one of claims 1 to 7, in which said fluid is injected into said bioreactor for about 5 minutes at about 20 minutes. 11. The method according to any one of claims 1 to 10, in which the Fluid is injected through a nozzle. 12. The method according to claim 11, wherein said nozzle includes a anterior portion and a posterior portion. 13. The method according to claim 11 or 12, wherein said nozzle has a equivalent diameter from about 5 mm to about 127 mm. 14. The method according to claim 11 or 12, wherein said nozzle has a equivalent diameter from about 20 mm to about 100 mm. 15. The method according to claim 11 or 12, wherein said nozzle has a equivalent diameter from about 20 mm to about 80 mm. 16. The method according to claim 11 or 12, wherein said nozzle has a equivalent diameter from about 20 mm to about 60 mm. 17. The method according to claim 11 or 12, wherein said nozzle has a equivalent diameter from about 20 mm to about 30 mm. 18. The method according to any one of claims 11 to 17, in which said nozzle is provided with a cap. 19. The method according to claim 18, wherein said plug of said nozzle is flat, domed, conical or bevelled. 20. The method according to claim 18 or 19, wherein said stopper of said nozzle comprises one or more orifices. 21. The method according to any one of claims 11 to 20, in which said rear portion of said nozzle includes one or more orifices.

Date Received/Date Received 2022-04-29 22. The method according to claim 20 or 21, wherein each orifice has a equivalent diameter from about 1 mm to about 127 mm. 23. The method according to claim 20 or 21, wherein each orifice has a equivalent diameter from about 5 mm to about 100 mm. 24. The method according to claim 20 or 21, wherein each orifice has a equivalent diameter from about 10 mm to about 80 mm. 25. The method according to claim 20 or 21, wherein each orifice has a equivalent diameter from about 20 mm to about 60 mm. 26. The method according to claim 20 or 21, wherein each orifice has a equivalent diameter from about 20 mm to about 30 mm. 27. The method according to any one of claims 11 to 26, in which said fluid is injected in a longitudinal, lateral and/or diagonal relative to said nozzle. 28. The method according to any one of claims 11 to 27, in which the nozzle is a motorized nozzle or a nozzle that can be manipulated by an articulated arm or a user. 29. The method according to any one of claims 11 to 28, in which said mud is separated by contacting said mud with said nozzle. 30. The method according to any one of claims 11 to 28, in which said sludge is separated by contacting said sludge with said nozzle by performing movements back and forth with said nozzle. 31. The method according to any one of claims 11 to 30, in which said nozzle is moved inside said bioreactor.

Date Received/Date Received 2022-04-29 32. The method according to any one of claims 11 to 31, in which said nozzle is moved and/or oriented towards one or more zones comprising of the mud accumulated there. 33. The method according to any one of claims 11 to 32, in which said nozzle is moved and/or oriented towards one or more zones comprising of the sludge accumulated therein simultaneously with said injection. 34. The method according to any one of claims 11 to 33, in which said nozzle is moved and/or oriented so as to contact mud accumulated at the surface, in and/or around the packing placed in said bioreactor. 35. The method according to any one of claims 11 to 34, in which said front end of said nozzle is connected to equipment injection of fluid. 36. The method according to one of claims 1 to 35, in which the fluid is a gas. 37. The method according to one of claims 1 to 35, in which the fluid is a liquid. 38. The method of claim 37, wherein said liquid is liquid recirculated. 39. The method according to one of claims 1 to 38, in which said injection of fluid and said sludge withdrawal take place simultaneously. 40. The method according to one of claims 1 to 39, further comprising a step of aerating the bottom of a reservoir of said bioreactor. 41. The method of claim 40, wherein said aeration has place before said fluid injection.

Date Received/Date Received 2022-04-29 42. The method of claim 40, wherein said aeration has place before said fluid injection and during said fluid injection. 43. The method of claim 40, wherein said aeration has place before said fluid injection, during said fluid injection and during said racking. 44. The method of claim 40, wherein said aeration has location during said injection of fluid and during said withdrawal. 45. The method of claim 40, wherein said aeration has location during said racking. 46. The method according to any one of claims 1 to 45, in which said withdrawal is carried out through a strainer. 47. The method according to claim 46, wherein said strainer includes a or more holes. 48. The method of claim 46, wherein said strainer includes 5 to 100 holes. 49. The method of claim 46, wherein said strainer includes 10 at 75 holes. 50. The method of claim 46, wherein said strainer includes 5 to 50 holes. 51. The method of claim 46, wherein said strainer includes 50 at 100 holes. 52. The method according to any one of claims 47 to 51, in which said orifice or orifices has/have an equivalent diameter of approximately 2 mm at about 100mm.

Date Received/Date Received 2022-04-29 53. The method according to any one of claims 47 to 51, in which said orifice or orifices has/have an equivalent diameter of about 7 mm at about 75 mm. 54. The method according to any one of claims 47 to 51, in which said orifice or orifices has/have an equivalent diameter of approximately 10 mm at about 60mm. 55. The method according to any one of claims 47 to 51, in which said orifice or orifices has/have an equivalent diameter of about 12 mm at about 40mm. 56. The method according to any one of claims 47 to 55, in which the strainer has a beveled shape. 57. The method according to any one of claims 47 to 56, in which said strainer is connected to a suction mechanism. 58. The method according to any one of claims 47 to 56, in which said strainer is connected to a suction mechanism via a conduit suction. 59. The method according to claim 58, wherein said mechanism suction is a pump or sewer truck. 60. The method according to any one of claims 1 to 59, in which said withdrawal takes place at a withdrawal rate of approximately 300 L/min at approximately 1500 L/min. 61. The method according to any one of claims 1 to 59, in which said withdrawal takes place at a withdrawal rate of approximately 475 L/min at approximately 1215 L/min. 62. The method according to any one of claims 1 to 59, in which said withdrawal takes place at a suction rate of about 8 L/sec at about 20 L/sec.

Date Received/Date Received 2022-04-29 63. The method according to any one of claims 1 to 59, in which said withdrawal takes place at a suction rate at the orifices of approximately 1.2 m/sec at about 2.5 m/sec. 64. The method according to any one of claims 1 to 63, in which said packing is of the non-moving type. 65. The method according to any one of claims 1 to 63, in which said packing is of the non-moving flexible type. 66. The method of claim 65, wherein the packing of kind non-moving flexible is a bundled or entangled filamentous packing. 67. Kit for removing mud accumulated on the surface, in and/or around of one packing of a bioreactor for the treatment of waste and/or accumulated water in said bioreactor, said kit comprising:
an injection nozzle, fluid injection equipment, a draw-off strainer, a suction duct, and a suction mechanism, said strainer being sized to selectively draw off sludge separated from said packing. 68. The kit according to claim 67, further comprising a device to ventilate the bottom of a tank of said bioreactor. 69. The kit according to claim 67 or 68, wherein said nozzle includes a anterior portion and a posterior portion. 70. The kit according to any one of claims 67 to 69, wherein said nozzle has an equivalent diameter of about 5 mm to about 127 mm.

Date Received/Date Received 2022-04-29 71. The kit according to any one of claims 67 to 69, wherein said nozzle has an equivalent diameter of about 10 mm to about 100 mm. 72. The kit according to any one of claims 67 to 69, wherein said nozzle has an equivalent diameter of about 20 mm to about 80 mm. 73. The kit according to any one of claims 67 to 69, wherein said nozzle has an equivalent diameter of about 20 mm to about 60 mm. 74. The kit according to any one of claims 67 to 69, wherein said nozzle has an equivalent diameter of about 20 mm to about 30 mm 75. The kit according to any one of claims 67 to 74, wherein said nozzle is fitted with a cap. 76. The kit according to claim 75, wherein said cap of said nozzle is flat, domed, conical or bevelled. 77. The kit according to claim 75 or 76, wherein said plug of said nozzle comprises one or more orifices. 78. The kit according to any one of claims 69 to 77, wherein said rear portion of said nozzle comprises one or more orifices. 79. The kit according to claim 77 or 78, wherein each orifice has a diameter equivalent of about 1 mm to about 127 mm. 80. The kit according to claim 77 or 78, wherein each orifice has a diameter equivalent of about 5 mm to about 100 mm. 81. The kit according to claim 77 or 78, wherein each orifice has a diameter equivalent of about 10 mm to about 80 mm. 82. The kit according to claim 77 or 78, wherein each orifice has a diameter equivalent of about 20 mm to about 50 mm.

Date Received/Date Received 2022-04-29 83. The kit according to claim 77 or 78, wherein each orifice has a diameter equivalent of about 20 mm to about 30 mm. 84. The kit according to any one of claims 67 to 83, wherein said strainer includes one or more orifices. 85. The kit according to any one of claims 67 to 83, wherein said strainer has 5 to 100 holes. 86. The kit according to any one of claims 67 to 83, wherein said strainer has 10 to 75 holes. 87. The kit according to any one of claims 67 to 83, wherein said strainer has 5 to 50 holes. 88. The kit according to any one of claims 67 to 83, wherein said strainer has 50 to 100 orifices. 89. The kit according to any one of claims 84 to 88, wherein said orifice or said orifices has/have an equivalent diameter of from about 2 mm to about 100 mm. 90. The kit according to any one of claims 84 to 88, wherein said orifice or said orifices has/have an equivalent diameter of about 7 mm to about 75 mm. 91. The kit according to any one of claims 84 to 88, wherein said orifice or said orifices has/have an equivalent diameter of about 10 mm to about 60 mm. 92. The kit according to any one of claims 84 to 88, wherein said orifice or said orifices has/have an equivalent diameter of from about 12 mm to about 40 mm. 93. The kit according to any one of claims 67 to 92, wherein the strainer has a bevelled shape. 94. The kit according to any one of claims 67 to 93, wherein the fluid is a gas.

Date Received/Date Received 2022-04-29 95. The kit according to any one of claims 67 to 93, wherein the fluid is a liquid, optionally a recirculated liquid. 96. The kit according to any one of claims 67 to 95, wherein fluid injection equipment is a compressor, blower or pump. 97. Unit for withdrawing sludge accumulated on the surface, in and/or around packing in a bioreactor for waste water treatment, and/or accumulated in said bioreactor, said unit comprising:
an injection nozzle intended to inject a fluid to separate at least part of said accumulated sludge, fluid injection equipment intended to be in fluid communication with said nozzle, a draw-off strainer, a suction mechanism, and a suction conduit for fluid communication with said strainer and said suction mechanism, said strainer being sized to selectively draw off sludge separated from said packing. 98. The unit according to claim 97, further comprising a device to ventilate the bottom of a tank of said bioreactor. 99. The unit according to claim 97 or 98, wherein said nozzle includes a anterior portion and a posterior portion. 100. The unit according to any one of claims 97 to 99, wherein said nozzle has an equivalent diameter of about 5 mm to about 127 mm. 101. The unit according to any one of claims 97 to 99, wherein said nozzle has an equivalent diameter of about 10 mm to about 100 mm.

Date Received/Date Received 2022-04-29 102. The unit according to any one of claims 97 to 99, wherein said nozzle has an equivalent diameter of about 20 mm to about 80 mm. 103. The unit according to any one of claims 97 to 99, wherein said nozzle has an equivalent diameter of about 20 mm to about 60 mm. 104. The unit according to any one of claims 97 to 99, wherein said nozzle has an equivalent diameter of about 20 mm to about 30 mm. 105. The unit according to any one of claims 97 to 104, wherein said nozzle is fitted with a cap. 106. The unit according to claim 105, wherein said plug of said nozzle is flat, domed, conical or bevelled. 107. The unit according to any one of claims 105 to 106, in which said cap of said nozzle comprises one or more orifices. 108. The unit according to any one of claims 99 to 107, wherein the rear portion of said nozzle comprises one or more orifices. 109. The unit according to claim 107 or 108, wherein each port has a equivalent diameter from about 1 mm to about 127 mm. 110. The unit according to claim 107 or 108, wherein each port has a equivalent diameter from about 5 mm to about 100 mm. 111. The unit according to claim 107 or 108, wherein each port has a equivalent diameter from about 10 mm to about 80 mm. 112. The unit according to claim 107 or 108, wherein each port has a equivalent diameter from about 20 mm to about 50 mm. 113. The unit according to claim 107 or 108, wherein each port has a equivalent diameter from about 20 mm to about 30 mm.

Date Received/Date Received 2022-04-29 114. The unit according to any one of claims 97 to 113, wherein said strainer includes one or more orifices. 115. The unit according to any one of claims 97 to 113, wherein said strainer has 5 to 100 holes. 116. The unit according to any one of claims 97 to 113, wherein said strainer has 10 to 75 holes. 117. The unit according to any one of claims 97 to 113, wherein said strainer has 5 to 50 holes. 118. The unit according to any one of claims 97 to 113, wherein said strainer has 50 to 100 orifices. 119. The unit according to any one of claims 114 to 118, in which said orifice or said orifices has/have an equivalent diameter of approximately 2 mm to about 100 mm. 120. The unit according to any one of claims 114 to 118, in which said orifice or said orifices has/have an equivalent diameter of about 7 mm to about 75mm. 121. The unit according to any one of claims 114 to 118, in which said orifice or said orifices has/have an equivalent diameter of about 10 mm to about 60 mm. 122. The unit according to any one of claims 114 to 118, in which said orifice or said orifices has/have an equivalent diameter of approximately 12 mm to about 40 mm. 123. The unit according to any one of claims 97 to 122, wherein the strainer has a beveled shape. 124. The unit according to any one of claims 97 to 123, wherein the fluid is a gas.

Date Received/Date Received 2022-04-29 125. The unit according to any one of claims 97 to 123, wherein the fluid is a liquid, optionally a recirculated liquid. 126. The unit according to any one of claims 97 to 125 or the kit according to one any of claims 67 to 96, wherein the packing is a filling non-mobile. 127. The unit according to any one of claims 97 to 125 or the kit according to one any of claims 67 to 96, wherein the packing is a filling non-moving hose. 128. The method according to claim 36, the kit according to claim 94 or the unit according to claim 124, wherein the gas comprises air. 129. The method according to any one of claims 37 and 38, the kit according to claim 95 or the unit according to claim 125, wherein the liquid includes some water. 130. Method for cleaning a bioreactor and/or its lining including:
the implementation of the sludge separation method as defined in one any of claims 1 to 66; and the withdrawal of said separated sludge. 131. Method for cleaning a bioreactor and/or its lining including:
the separation of at least a portion of a sludge accumulated in said bioreactor and/or on the surface, in and/or around a packing of said bioreactor; and the implementation of the sludge extraction method as defined in moon any of claims 1 to 66.

Date Received/Date Received 2022-04-29 132. The method of claim 35, wherein said equipment injection fluid is a compressor, blower or pump. 133. The unit according to any one of claims 97 to 127, wherein said fluid injection equipment is a compressor, blower or pump. 134. The kit according to any one of claims 67 to 96, wherein the suction mechanism is a pump or sewer truck. 135. The unit according to any one of claims 97 to 127, wherein the suction mechanism is a pump or sewer truck.

Date Received/Date Received 2022-04-29