CN104188722B - Laser biology effect measurement device - Google Patents
Laser biology effect measurement device Download PDFInfo
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- CN104188722B CN104188722B CN201410459592.3A CN201410459592A CN104188722B CN 104188722 B CN104188722 B CN 104188722B CN 201410459592 A CN201410459592 A CN 201410459592A CN 104188722 B CN104188722 B CN 104188722B
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- 230000000694 effects Effects 0.000 title claims abstract description 16
- 238000005259 measurement Methods 0.000 title claims abstract description 14
- 239000000523 sample Substances 0.000 claims abstract description 39
- 238000002474 experimental method Methods 0.000 claims abstract description 18
- 230000005540 biological transmission Effects 0.000 claims abstract description 17
- 230000005855 radiation Effects 0.000 claims abstract description 16
- 238000009529 body temperature measurement Methods 0.000 claims abstract description 14
- 239000013307 optical fiber Substances 0.000 claims description 7
- 229910001220 stainless steel Inorganic materials 0.000 claims description 6
- 239000010935 stainless steel Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 238000011160 research Methods 0.000 abstract description 12
- 230000004071 biological effect Effects 0.000 abstract description 11
- 230000003287 optical effect Effects 0.000 abstract description 8
- 241000931526 Acer campestre Species 0.000 abstract description 4
- 238000002647 laser therapy Methods 0.000 abstract description 3
- 210000004185 liver Anatomy 0.000 description 5
- 230000023597 hemostasis Effects 0.000 description 4
- 238000009834 vaporization Methods 0.000 description 4
- 230000008016 vaporization Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000000342 Monte Carlo simulation Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
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Abstract
The invention discloses a kind of laser biology effect measurement device, comprise laser probe, laser probe fixed mount, infrared radiation temperature measurement device, biological tissue's fixed mount, microscopic system, transmission laser beam channel and experiment fixed platform, laser probe is fixed on laser probe fixed mount, biological tissue to be measured is fixed on biological tissue's fixed mount, microscopic system is in the dead astern of biological tissue's fixed mount, and transmission laser beam channel is positioned at the dead astern of microscopic system; The side that infrared radiation temperature measurement device is arranged on biological tissue's fixed mount changes in order to the real time temperature of measuring biological tissue. The present invention carries out biological effect research applicable to multiple Laser Experiments such as different optical maser wavelength, mode of operation, laser powers, for laser therapy provides directly perceived, comprehensive and predictable information, the present invention is simple in structure, easy to operate, stable performance, for Study of Laser biological effect provides convenience.
Description
Technical field
The present invention relates to the biological effect research of a kind of laser and biological tissue, in particular for laser biology effect measurement apparatus and method, belong to the heat conducting research of biological tissue.
Background technology
The biological effect of laser and biological tissue is that laser is in one of importance of medically applying, along with laser is in medically application more and more widely, the biological effect research of laser and tissue will cause the irreversible damage of biological tissue after causing gradually people's attention biological tissue absorbing laser energy, thereby reach the object for the treatment of, as solidify, vaporization, cutting, hemostasis etc. the biological effect of laser and biological tissue depends primarily on the parameters such as the power of sharp light wavelength, mode of operation, laser. but the research majority of laser biology effect has only carried out single wavelength laser action in biological tissue's theory or experimental study at present, although some a little research has been applied to clinical operation, but often can only complete and solidify in clinical, vaporization, cutting, a function in hemostasis etc., along with continuous renewal and the development of technology, the continuous expansion of indication therapeutic domain, clinical operation to laser medical equipment require more and more diversified, the needs that the laser medical equipment of single wavelength can not meet modern micro-wound surgical operation will complete whole operation and assist and just can complete whole operation toward other apparatus of contact needs.
Although laser has been obtained many achievements in clinical practice field, also need to carry out theoretical integration, particularly on Quantitative study basis, improve predictability, the safety and reliability of application. At present laser and biological tissue's photothermy are mainly carried out to research from two aspects: the one, the Transport (bio-photon) of photon biological tissue, the 2nd, the conduction (biothermics) of photoproduction heat in biological tissue. In tissue, the theoretical and simulation of photon transmission is the emphasis of Biomedical Photonics in recent years, the optical property of biological tissue is all launched to more deep research both at home and abroad, the mainly analytic method based on transmission theory and Monte-Carlo simulation, many researchers are from it, light in quantitative description tissue distributes, and then obtain the heat distribution in biological tissue, obtain some significant results. Particularly opthalmic optics and skin optical, adopts advanced detection technique to obtain the optical parametrics such as the refractive index of tissue, diffuse reflectance, fluorescence spectrum. Clinical practice is moved towards in optical non-destructive detection and diagnosis just gradually. The problem of the hot conduction studies of biological tissue, is according to the characteristic of tissue, sets up heat transfer model, understands heat and fire damage situation that laser causes in biological tissue.
Summary of the invention
Technical problem to be solved by this invention is the defect existing for prior art, provides a kind of and can set up heat transfer model according to the characteristic of biological tissue, understands the heat that causes of laser and the biological effect measurement mechanism of fire damage situation in biological tissue.
For solving this technical problem, the invention provides a kind of laser biology effect measurement device, comprise laser probe, laser probe fixed mount, infrared radiation temperature measurement device, biological tissue's fixed mount, microscopic system, transmission laser beam channel and experiment fixed platform, described laser probe fixed mount, infrared radiation temperature measurement device, biological tissue's fixed mount, microscopic system, transmission laser beam channel is fixed in experiment fixed platform, laser probe is fixed on laser probe fixed mount, biological tissue to be measured is fixed on biological tissue's fixed mount, the center of biological tissue's fixed mount and laser probe fixed mount at grade, the laser action of sending from laser probe is on biological tissue, described microscopic system is in the dead astern of biological tissue's fixed mount, the object lens center of microscopic system and the center of biological tissue's fixed mount are in same level, transmission laser beam channel is positioned at the dead astern of microscopic system, the side that infrared radiation temperature measurement device is arranged on biological tissue's fixed mount changes in order to the real time temperature of measuring biological tissue, and the table top of experiment fixed platform is provided with multiple equidistant screwed holes for regulating the spacing between each fixed mount.
Described laser probe is for directly going out optical fiber or side goes out optical fiber.
Described laser probe fixed mount forms by fixing and lock two parts, is made up of stainless steel material.
Described biological tissue fixed mount is scalable circle folder, can fix the biological tissue of different sizes, shape.
Described microscopic system is biomicroscope.
Described experiment fixed platform is stainless steel table top,
Beneficial effect: the light that the present invention has set up in a set of quantitative measurment tissue distributes, and then obtain the measurement mechanism of the heat distribution in biological tissue, can be to the vaporization width of tissue by laser, solidify width, evaporating depth, the corresponding relation of solidifying layer thickness carries out careful research, applicable to different optical maser wavelength, mode of operation, biological effect research is carried out in the multiple Laser Experiments such as the power of laser, for laser therapy provides directly perceived, comprehensive and predictable information, for solid foundation is established in clinical practice, the present invention is simple in structure, easy to operate, stable performance and be applicable to different wave length and different biological tissues, for Study of Laser biological effect provides convenience.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
In figure: 1 laser probe, 2 laser probe fixed mounts, 3 infrared radiation temperature measurement devices, 4 biological tissue's fixed mounts, 5 microscopic systems, 6 transmission laser beam channels, 7 experiment fixed platforms.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is specifically described.
Figure 1 shows that structural representation of the present invention.
The present invention includes laser probe 1, laser probe fixed mount 2, infrared radiation temperature measurement device 3, biological tissue's fixed mount 4, microscopic system 5, transmission laser beam channel 6 and experiment fixed platform 7, described laser probe fixed mount 2, infrared radiation temperature measurement device 3, biological tissue's fixed mount 4, microscopic system 5, transmission laser beam channel 6 are fixed in experiment fixed platform 7.
Described laser probe 1 is for directly going out optical fiber or side goes out optical fiber etc.
Laser probe fixed mount 2 forms by fixing and lock two parts, is made up of stainless steel material, is used for fixing difform laser probe 1.
Described biological tissue fixed mount 4 is scalable circle folder, can fix the biological tissue of different sizes, shape.
Described laser probe 1 is fixed on laser probe fixed mount 2, biological tissue to be measured is fixed on biological tissue's fixed mount 4, the center of biological tissue's fixed mount 4 and laser probe fixed mount 2 at grade, the laser action of sending from laser probe 1 on biological tissue, to biological tissue gasify, cutting, hemostasis etc.
Described microscopic system 5 is in the dead astern of biological tissue's fixed mount 4, and the object lens center of microscopic system 5 and the center of biological tissue's fixed mount 4 are in same level.
Described microscopic system 5 is biomicroscope.
Described transmission laser beam channel 6 is positioned at the dead astern of microscopic system 5, and the residue laser seeing through from biological tissue is derived through transmission laser beam channel 6, and the laser after derivation is measured with laser power meter, in order to determine the laser loss rate after effect.
The side that described infrared radiation temperature measurement device 3 is arranged on biological tissue's fixed mount 4 changes in order to the real time temperature of measuring biological tissue.
The table top of described experiment fixed platform 7 is provided with multiple equidistant screwed holes for regulating the spacing between each fixed mount.
Described experiment fixed platform 7 is stainless steel table top,
Operation principle of the present invention:
The laser action of sending from laser probe is on biological tissue, to biological tissue gasify, cutting, hemostasis etc., biological tissue after effect is observed by microscope, utilize infrared radiation temperature measurement device to measure the real time temperature of biological tissue simultaneously, the laser that sees through biological tissue spreads out of by transmission laser beam channel, and is measured in real time by laser power meter.
Embodiments of the invention
Experiment is 1.32nm by optical maser wavelength;
Laser probe 1 is for directly going out optical fiber, and laser probe 1 is fixed on laser probe fixed mount 2;
Microscopic system 5 is ZEISSAxioskop40, and is fixed on fixed frame for microscope;
Biological tissue is the liver of pig, and is fixed on biological tissue's fixed mount 4;
Infrared radiation temperature measurement device 3 is HL infrared radiation thermometer.
1.32nm laser sends from laser probe 1 on the liver that acts on pig, liver to pig cuts, observe the cutting effect of 1.32nm laser to pig liver under different capacity by microscopic system 5, record variations in temperature real-time in experimentation by infrared radiation thermometer 3, the laser appearing from pig liver is derived through transmission laser beam channel 6, and is measured in real time by laser power meter.
From whole experimentation, the method is carried out laser biology effect study, convenient, practical.
The light that the present invention has set up in a set of quantitative measurment tissue distributes, and then obtain the measurement mechanism of the heat distribution in biological tissue, can be to the vaporization width of tissue by laser, solidify width, evaporating depth, the corresponding relation of solidifying layer thickness carries out careful research, applicable to different optical maser wavelength, mode of operation, biological effect research is carried out in the multiple Laser Experiments such as the power of laser, for laser therapy provides directly perceived, comprehensive and predictable information, for solid foundation is established in clinical practice, the present invention is simple in structure, easy to operate, stable performance and be applicable to different wave length and different biological tissues, for Study of Laser biological effect provides convenience.
The above-mentioned embodiment of the present invention, just illustrates, and is not only, and change within the scope of the present invention all or that be equal in scope of the present invention is all surrounded by the present invention.
Claims (6)
1. a laser biology effect measurement device, it is characterized in that: comprise laser probe (1), laser probe fixed mount (2), infrared radiation temperature measurement device (3), biological tissue's fixed mount (4), microscopic system (5), transmission laser beam channel (6) and experiment fixed platform (7), described laser probe fixed mount (2), infrared radiation temperature measurement device (3), biological tissue's fixed mount (4), microscopic system (5), transmission laser beam channel (6) is fixed in experiment fixed platform (7), laser probe (1) is fixed on laser probe fixed mount (2), biological tissue to be measured is fixed on biological tissue's fixed mount (4), the center of biological tissue's fixed mount (4) and laser probe fixed mount (2) at grade, the laser action of sending from laser probe (1) is on biological tissue, described microscopic system (5) is in the dead astern of biological tissue's fixed mount (4), the center of the object lens center of microscopic system (5) and biological tissue's fixed mount (4) is in same level, transmission laser beam channel (6) is positioned at the dead astern of microscopic system (5), the side that infrared radiation temperature measurement device (3) is arranged on biological tissue's fixed mount (4) changes in order to the real time temperature of measuring biological tissue, and the table top of experiment fixed platform (7) is provided with multiple equidistant screwed holes for regulating the spacing between each fixed mount.
2. laser biology effect measurement device according to claim 1, is characterized in that: described laser probe (1) is for directly going out optical fiber or side goes out optical fiber.
3. laser biology effect measurement device according to claim 1, is characterized in that: described laser probe fixed mount (2) forms by fixing and lock two parts, is made up of stainless steel material.
4. laser biology effect measurement device according to claim 1, is characterized in that: described biological tissue fixed mount (4), for scalable circle folder, can be fixed the biological tissue of different sizes, shape.
5. laser biology effect measurement device according to claim 1, is characterized in that: described microscopic system (5) is biomicroscope.
6. according to the laser biology effect measurement device described in claim 1-5 any one, it is characterized in that: described experiment fixed platform (7) is stainless steel table top.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410459592.3A CN104188722B (en) | 2014-09-10 | 2014-09-10 | Laser biology effect measurement device |
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| CN201410459592.3A CN104188722B (en) | 2014-09-10 | 2014-09-10 | Laser biology effect measurement device |
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| CN104188722A CN104188722A (en) | 2014-12-10 |
| CN104188722B true CN104188722B (en) | 2016-05-11 |
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| JP3757854B2 (en) * | 2001-12-06 | 2006-03-22 | 株式会社島津製作所 | Method and apparatus for analyzing sample containing a plurality of fluorescent substances |
| US20070237676A1 (en) * | 2002-04-15 | 2007-10-11 | Colton Jonathan S | Polymer micro-cantilever with probe tip and method for making same |
| US7524672B2 (en) * | 2004-09-22 | 2009-04-28 | Sandia Corporation | Microfluidic microarray systems and methods thereof |
| CN101776791B (en) * | 2010-01-08 | 2012-09-26 | 华中科技大学 | Light stimulation device |
| WO2011144212A1 (en) * | 2010-05-21 | 2011-11-24 | Chemometec A/S | A compact dark field light source and dark field image analysis at low magnification |
| CN204158493U (en) * | 2014-09-10 | 2015-02-18 | 山东省药物研究院 | Time transfer by laser measuring device |
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Inventor after: Wu Hui Inventor before: Li Sheng Inventor before: Pang Kai Inventor before: Xia Mei Inventor before: Gao Dehai |
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Effective date of registration: 20171201 Address after: 255000 Shandong high tech Zone, Zibo, Shandong Road, No. 51, high polymer materials innovation park B block, room 2011 Patentee after: Shandong Weikang Medical Inspection Co. Ltd. Address before: 250200 Zhangqiu, Shandong, No. ten East Road, No. 6866, No. Patentee before: SHANDONG PROVINCE INSTITUTE OF PHARMACEUTICAL RESEARCH |