CN104304173B - Method for quick and the system of chicken embryo to illumination stress reaction - Google Patents
Method for quick and the system of chicken embryo to illumination stress reaction Download PDFInfo
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- 241000287828 Gallus gallus Species 0.000 title claims abstract description 102
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000006243 chemical reaction Methods 0.000 title claims description 9
- 238000005286 illumination Methods 0.000 title abstract 2
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 16
- 230000003938 response to stress Effects 0.000 claims abstract description 12
- 230000004044 response Effects 0.000 claims abstract description 11
- 229940012466 egg shell membrane Drugs 0.000 claims abstract description 8
- 238000001514 detection method Methods 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 14
- 239000004744 fabric Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000002474 experimental method Methods 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000011160 research Methods 0.000 abstract description 9
- 230000008859 change Effects 0.000 abstract description 7
- 244000144977 poultry Species 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 238000013461 design Methods 0.000 abstract description 2
- 230000012447 hatching Effects 0.000 abstract description 2
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 235000013330 chicken meat Nutrition 0.000 description 70
- 235000013594 poultry meat Nutrition 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 230000003760 hair shine Effects 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000005236 sound signal Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000009374 poultry farming Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
- A01K45/007—Injecting or otherwise treating hatching eggs
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Abstract
本发明公开了一种鸡胚对光照应激反应的快速检测方法及系统。搭建一个柱形实验区域，与孵化器的环境保持一致，保持恒温和恒湿，不透光的柱形实验区域内中部装有光源，将鸡胚放入柱形实验区域，在鸡胚气室小孔上装有声音传感器，其粘贴在小孔内的蛋壳膜上，由声音传感器接收到的信号转换得到鸡胚的心跳信号，通过调节光源的强度和波长以及鸡胚的高度位置改变鸡胚的光照条件，多次检测然后相比较得到鸡胚对光照的应激反应。本发明主要用于监测鸡胚对光照的应激反应，设计简单，结构合理；保证了孵化过程中温度、湿度一致，改变LED光源的波长和光强，得到不同光照条件下鸡胚心跳信号的变化，即是应激对家禽的影响研究的深化。
The invention discloses a method and system for quickly detecting chicken embryo's response to light stress. Build a columnar experimental area, which is consistent with the environment of the incubator, and maintain constant temperature and humidity. A light source is installed in the middle of the opaque columnar experimental area, and chicken embryos are placed in the columnar experimental area. The hole is equipped with a sound sensor, which is pasted on the eggshell membrane in the small hole, and the signal received by the sound sensor is converted to obtain the heartbeat signal of the chicken embryo. Illumination conditions were measured multiple times and then compared to obtain the stress response of chicken embryos to light. The invention is mainly used for monitoring the stress response of chicken embryos to light, with simple design and reasonable structure; it ensures consistent temperature and humidity during the hatching process, changes the wavelength and light intensity of the LED light source, and obtains heartbeat signals of chicken embryos under different lighting conditions. Change, that is, the deepening of research on the effects of stress on poultry.
Description
技术领域 technical field
本发明涉及一种鸡胚的检测方法和系统，尤其是涉及一种鸡胚对光照应激反应的快速检测方法及系统。 The invention relates to a chicken embryo detection method and system, in particular to a rapid detection method and system for chicken embryo response to light stress.
背景技术 Background technique
在当前的禽类养殖中，禽类产业的集约化本身就是增加应激反应的过程。由于应激因素的作用，使禽类机体内的生理环境和适应性，稳定性改变，产生了新的适应，以维持生命和恢复功能。在禽类的应激方面，特别是对鸡的应激研究，对于鸡的生产、生理、行为有着重大影响。而在鸡胚时就对鸡的应激反应进行研究，对以后鸡的成长有着更加创新性的作用。LED光源凭借体积小，寿命长，多变幻等优点已被广泛应用于各种指示、显示、装饰、背光源、普通照明和城市夜景等各个领域。 In current poultry farming, the intensification of the poultry industry is itself a process of increasing the stress response. Due to the effect of stress factors, the physiological environment and adaptability and stability of the poultry body are changed, and new adaptations are produced to maintain life and restore functions. In terms of stress in poultry, especially the research on stress in chickens, it has a significant impact on the production, physiology and behavior of chickens. However, studying the stress response of chickens in the embryo will have a more innovative effect on the growth of chickens in the future. LED light sources have been widely used in various fields such as indications, displays, decorations, backlights, general lighting, and urban night scenes due to their small size, long life, and versatility.
目前现有研究模式中，学者们的研究多是单独的对鸡胚心跳检测的研究，没有对环境的界定，或是对象为成熟的鸡在不同光照条件下生理活动的研究，缺少在环境的界定下对鸡胚心跳检测的研究以及鸡胚在不同光照条件下生理活动的研究。 In the current research mode, most of the scholars' researches are solely on the detection of the heartbeat of chicken embryos, without defining the environment, or the research on the physiological activities of mature chickens under different light conditions, lacking in the environment. The research on the heartbeat detection of chicken embryos and the physiological activities of chicken embryos under different light conditions are defined.
发明内容 Contents of the invention
为了能够解决鸡胚心跳检测的研究问题，本发明的目的在于提供一种鸡胚对光照应激反应的快速检测方法及系统，检测对外界环境光照条件应激反应。 In order to be able to solve the research problem of chicken embryo heartbeat detection, the purpose of the present invention is to provide a method and system for rapid detection of chicken embryo's response to light stress, and to detect the stress response to external environmental light conditions.
本发明解决其技术问题所采用的技术方案是： The technical solution adopted by the present invention to solve its technical problems is:
一、一种鸡胚对光照应激反应的快速检测方法： One, a kind of rapid detection method of chicken embryo to light stress response:
搭建一个柱形实验区域，其环境和孵化器的环境保持一致，保持恒温和恒湿，柱形实验区域不透光，柱形实验区域内中部装有光源，将鸡胚放入柱形实验区域，鸡胚的高度位置可调节，在鸡胚的气室位置开有一小孔，小孔上装有用于检测鸡胚声音信号的声音传感器，声音传感器粘贴在小孔内的蛋壳膜上，由声音传感器接收到的信号转换得到鸡胚的心跳信号，调节光源的强度和波长以及鸡胚的高度位置改变鸡胚的光照条件，在不同鸡胚的光照条件下多次检测然后相比较得到鸡胚对光照的应激反应。 Build a cylindrical experimental area, its environment is consistent with the environment of the incubator, keep constant temperature and humidity, the cylindrical experimental area is opaque, the middle of the cylindrical experimental area is equipped with a light source, put the chicken embryo into the cylindrical experimental area, The height of the chicken embryo can be adjusted. There is a small hole in the position of the air chamber of the chicken embryo. The sound sensor used to detect the sound signal of the chicken embryo is installed on the small hole. The sound sensor is pasted on the eggshell membrane in the small hole. The received signal is converted to obtain the heartbeat signal of the chicken embryo, the intensity and wavelength of the light source and the height and position of the chicken embryo are adjusted to change the lighting conditions of the chicken embryo, and the light conditions of the chicken embryo are tested multiple times under different lighting conditions of the chicken embryo and compared to obtain the light emission of the chicken embryo. stress response.
所述的鸡胚的高度通过安装于柱形实验区域内的鸡胚固定架调节。 The height of the chicken embryos is regulated by a chicken embryo fixing frame installed in the cylindrical experiment area.
所述的柱形实验区域的外侧采用不透光材料遮盖以隔离外界光照。 The outside of the columnar experimental area is covered with opaque material to isolate external light.
所述的不透光材料为荧光布或者遮光布。 The opaque material is fluorescent cloth or light-shielding cloth.
二、一种鸡胚对光照应激反应的快速检测系统： 2. A rapid detection system for chicken embryo response to light stress:
包括柱形实验区域、光源固定架、光源控制器、光源声音传感器、AD转换电路、计算机、温度计、恒温控制装置和鸡胚固定架；柱形实验区域置于恒温控制装置中，外侧面均覆盖有不透光材料以隔离外界光照，柱形实验区域内安装有温度计；光源通过光源固定架安装在柱形实验区域内侧壁中部，并与柱形实验区域外的光源控制器连接，通过光源控制器调节波长和光强；鸡胚固定架安装在柱形实验区域的中心，鸡胚放置于鸡胚固定架上，鸡胚的气室位置开有一小孔，小孔上装有用于检测鸡胚的声音传感器，声音传感器粘贴在小孔内的蛋壳膜上，声音传感器经AD转换电路与计算机连接。 Including cylindrical experimental area, light source fixing frame, light source controller, light source sound sensor, AD conversion circuit, computer, thermometer, constant temperature control device and chicken embryo fixing frame; the cylindrical experimental area is placed in the constant temperature control device, and the outer surface is covered There are opaque materials to isolate the external light, and a thermometer is installed in the cylindrical experimental area; the light source is installed in the middle of the inner wall of the cylindrical experimental area through the light source fixing frame, and is connected with the light source controller outside the cylindrical experimental area, controlled by the light source Adjust the wavelength and light intensity; the chicken embryo fixing frame is installed in the center of the cylindrical experimental area, the chicken embryo is placed on the chicken embryo fixing frame, and a small hole is opened in the air chamber of the chicken embryo, and the small hole is equipped with a detection device for the chicken embryo. The sound sensor is pasted on the eggshell membrane in the small hole, and the sound sensor is connected with the computer through an AD conversion circuit.
所述的声音传感器为驻极体传声器。 The sound sensor is an electret microphone.
所述的恒温控制装置为保持恒温的水槽。 The constant temperature control device is a water tank for maintaining constant temperature.
所述的鸡胚固定架为能升降的固定架。 The chicken embryo fixing frame is a lifting frame.
所述的不透光材料为荧光布或者遮光布。 The opaque material is fluorescent cloth or light-shielding cloth.
本发明具有的有益效果是： The beneficial effects that the present invention has are:
1、本发明主要用于监测鸡胚对光照的应激反应，设计简单，结构合理。 1. The invention is mainly used for monitoring the stress response of chicken embryos to light, and has a simple design and a reasonable structure.
2、通过本发明系统，既保证了孵化过程中温度、湿度一致，实现了控制单一变量的原则，通过改变LED光源的波长和光强，可以得到不同光照条件下鸡胚心跳信号的变化，即是应激对家禽的影响研究的深化。 2. Through the system of the present invention, the temperature and humidity in the hatching process are guaranteed to be consistent, and the principle of controlling a single variable is realized. By changing the wavelength and light intensity of the LED light source, the change of the heartbeat signal of the chicken embryo under different lighting conditions can be obtained, that is, It is the deepening of research on the effects of stress on poultry.
3、本发明可调节蛋架调节鸡胚位置，由此可测得不同鸡胚位置的心跳，为以后不同鸡胚位置的进一步研究提供更精确的数据。 3. The present invention can adjust the egg rack to adjust the position of the chicken embryo, so that the heartbeat of different chicken embryo positions can be measured, and more accurate data can be provided for further research on different chicken embryo positions in the future.
附图说明 Description of drawings
图1是本发明系统的连接结构图。 Fig. 1 is a connection structure diagram of the system of the present invention.
图2是本发明测量方法的过程示意图。 Fig. 2 is a schematic diagram of the process of the measurement method of the present invention.
图中：1、柱形实验区域，2、光源固定架，3、光源控制器，4、光源，5、不透光材料，6、鸡胚，7、小孔，8、声音传感器，9、AD转换电路，10、计算机，11、温度计，12、水，13、恒温控制装置，14、鸡胚固定架。 In the figure: 1. Cylindrical experimental area, 2. Light source fixing frame, 3. Light source controller, 4. Light source, 5. Opaque material, 6. Chicken embryo, 7. Small hole, 8. Sound sensor, 9. AD conversion circuit, 10, computer, 11, thermometer, 12, water, 13, constant temperature control device, 14, chicken embryo fixing frame.
具体实施方式 detailed description
下面结合附图和实施例对本发明作进一步说明。 The present invention will be further described below in conjunction with drawings and embodiments.
如图2所示，本发明方法包括：首先搭建一个柱形实验区域，其环境和孵化器的环境保持一致，保持恒温和恒湿，柱形实验区域不透光，柱形实验区域内中部设有光源，将鸡胚放入柱形实验区域，鸡胚的高度位置可调节，在鸡胚的气室位置开有一小孔，小孔上装有用于检测鸡胚声音信号的声音传感器，声音传感器粘贴在小孔内的蛋壳膜上，由声音传感器接收到的信号可通过AD转换电路与计算机连接转换得到鸡胚的心跳信号，光源可连接光源控制器进行调节波长和光强度（即调节光源4的颜色、亮度），按实验要求调节光源的强度和波长以及鸡胚的高度位置改变鸡胚的光照条件，在不同鸡胚的光照条件下多次检测然后相比较得到鸡胚对光照的应激反应。 As shown in Figure 2, the method of the present invention comprises: first build a columnar experiment area, its environment and the environment of incubator keep consistent, keep constant temperature and humidity, columnar experiment area is opaque, the middle part is provided with in the columnar experiment area. Light source, put the chicken embryo into the cylindrical experimental area, the height of the chicken embryo can be adjusted, there is a small hole in the position of the air chamber of the chicken embryo, the small hole is equipped with a sound sensor for detecting the sound signal of the chicken embryo, and the sound sensor is pasted on the On the eggshell membrane in the small hole, the signal received by the sound sensor can be connected to the computer through the AD conversion circuit to convert the heartbeat signal of the chicken embryo, and the light source can be connected to the light source controller to adjust the wavelength and light intensity (that is, adjust the color of the light source 4 , brightness), according to the experimental requirements, adjust the intensity and wavelength of the light source and the height and position of the chicken embryo to change the lighting conditions of the chicken embryo, and then test and compare the stress response of the chicken embryo to light in multiple detections under different lighting conditions of the chicken embryo.
鸡胚的高度通过安装于柱形实验区域内的鸡胚固定架调节。 The height of the chicken embryo is adjusted by the chicken embryo holder installed in the cylindrical experimental area.
柱形实验区域的外侧采用不透光材料遮盖以隔离外界光照，不透光材料可采用荧光布或者遮光布。 The outside of the cylindrical experimental area is covered with opaque materials to isolate external light. The opaque materials can be fluorescent cloth or shading cloth.
如图1所示，本发明系统包括柱形实验区域1、光源固定架2、光源控制器3、光源4声音传感器8、AD转换电路9、计算机10、温度计11、恒温控制装置13和鸡胚固定架14；柱形实验区域1置于恒温控制装置13中，外侧面均覆盖有不透光材料5以隔离外界光照，柱形实验区域1内安装有温度计11；光源4通过光源固定架2固定安装在柱形实验区域1内侧壁中部，并与柱形实验区域1外的光源控制器3连接，通过光源控制器3调节波长和光强；鸡胚固定架14安装在柱形实验区域1的中心，鸡胚6放置于鸡胚固定架14上，鸡胚6的气室位置开有一小孔7，小孔上装有用于检测鸡胚的声音传感器8，声音传感器8粘贴在小孔内的蛋壳膜上，声音传感器8经AD转换电路9与计算机10连接。 As shown in Figure 1, the system of the present invention comprises a columnar experimental area 1, a light source fixing frame 2, a light source controller 3, a light source 4, a sound sensor 8, an AD conversion circuit 9, a computer 10, a thermometer 11, a constant temperature control device 13 and chicken embryos. Fixing frame 14; cylindrical experimental area 1 is placed in constant temperature control device 13, and the outer surface is covered with opaque material 5 to isolate external light. Thermometer 11 is installed in cylindrical experimental area 1; light source 4 passes through light source fixing frame 2 Fixedly installed in the middle of the inner wall of the cylindrical experimental area 1, and connected to the light source controller 3 outside the cylindrical experimental area 1, the wavelength and light intensity can be adjusted through the light source controller 3; the chicken embryo fixing frame 14 is installed in the cylindrical experimental area 1 The center of the chicken embryo 6 is placed on the chicken embryo fixing frame 14, and the position of the air chamber of the chicken embryo 6 has a small hole 7, and the sound sensor 8 for detecting the chicken embryo is housed on the small hole, and the sound sensor 8 is pasted in the small hole. On the eggshell membrane, the sound sensor 8 is connected with the computer 10 through the AD conversion circuit 9 .
优选的声音传感器8为驻极体传声器。 A preferred sound sensor 8 is an electret microphone.
优选地，柱形实验区域可采用烧杯，柱形实验区域能传导来自恒温控制装置13的热量维持恒温，恒温控制装置13采用保持恒温的水浴锅，水浴锅内装有水12，使得柱形实验区域1内的温度保持恒温，柱形实验区域1内通过温度计11显示温度。 Preferably, a beaker can be used in the cylindrical experimental area, and the cylindrical experimental area can conduct heat from the constant temperature control device 13 to maintain a constant temperature. The constant temperature control device 13 adopts a water bath that maintains a constant temperature, and water 12 is housed in the water bath, so that the cylindrical experimental area The temperature in 1 is maintained at a constant temperature, and the temperature is displayed by a thermometer 11 in the cylindrical experimental area 1 .
鸡胚固定架14为能升降的固定架，由于光源位置固定，通过升降鸡胚，使得光源照射到鸡胚的位置和角度能够进行调整，实现各种不同光照条件的检测要求。 The chicken embryo fixed frame 14 is a fixed frame that can be lifted. Because the position of the light source is fixed, the position and angle of the chicken embryo illuminated by the light source can be adjusted by lifting the chicken embryo, so as to realize the detection requirements of various lighting conditions.
优选的光源为LED光源，不透光材料可采用荧光布或者遮光布。 The preferred light source is an LED light source, and fluorescent cloth or shading cloth can be used as the opaque material.
本发明的实施例和实施过程如下： Embodiments of the present invention and implementation process are as follows:
如图1所示，柱形实验区域1采用能传导热量的烧杯，烧杯为1L，外侧均覆盖有不透光材料5为遮光布以隔离外界光照，并将其置于水浴锅中，烧杯内部设置有水银温度计11，通过调节水浴锅使得水银温度计的温度显示在37.5℃左右，光源4采用朗拓生物科技有限公司生产的波长亮度可调的LED灯，固定在烧杯侧壁，LED灯通过光源固定架2固定在烧杯1的侧壁上距离烧杯底部10cm，烧杯高15.5cm，调节光源控制器3使得LED4至波长560nm，光强30Lux，将第17天的胚胎在气室方向上敲开一个约1cm*1cm的小孔7，使驻极体传声器8刚好能安装在小孔7，将驻极体传声器8与蛋壳膜通过双面胶连接，调节鸡胚固定架高度14至距离烧杯底部5cm，使光源4照在鸡胚6顶部，观察记录计算机10信号，5分钟后再调节鸡胚固定架14高度至距离烧杯底部7.5cm，检测光源4照在鸡胚6中部时计算机的输出信号、同样过5分钟，调节鸡胚固定架14高度至距离烧杯底部10cm，检测光源4照在鸡胚6底部时信号并将同组三个信号整理记录得到第一组数据。改变光源4的波长至450nm，调节光源控制器至以上三个位置，得到第二组数据。在波长为560nm时改变光强至15Lux，调节光源控制器至以上三个位置，得到第三组数据，通过比较第一组和第二组数据得出不同光波长下的鸡胚心跳信号区别从而可以得到鸡胚对光波长的应激反应及其规律。通过比较第一组和第三组数据得出不同光强下的鸡胚心跳信号区别从而可以推出鸡胚对光强的应激规律。 As shown in Figure 1, the cylindrical experimental area 1 adopts a beaker capable of conducting heat, the beaker is 1L, and the outside is covered with opaque material 5 as a shading cloth to isolate the external light, and it is placed in a water bath. A mercury thermometer 11 is installed, and the temperature of the mercury thermometer is displayed at about 37.5°C by adjusting the water bath. The light source 4 is an LED lamp with adjustable wavelength and brightness produced by Langtuo Biotechnology Co., Ltd., which is fixed on the side wall of the beaker. The LED lamp passes through the light source The fixed frame 2 is fixed on the side wall of the beaker 1, 10cm away from the bottom of the beaker, and the height of the beaker is 15.5cm. Adjust the light source controller 3 so that the wavelength of LED4 is 560nm, and the light intensity is 30Lux. The small hole 7 of about 1cm*1cm, so that the electret microphone 8 can just be installed in the small hole 7, connect the electret microphone 8 and the eggshell membrane through double-sided tape, and adjust the height of the chicken embryo fixing frame to 14 to the distance from the bottom of the beaker 5cm, make the light source 4 shine on the top of the chicken embryo 6, observe and record the signal of the computer 10, adjust the height of the chicken embryo fixing frame 14 to a distance of 7.5cm from the bottom of the beaker after 5 minutes, and detect the output signal of the computer when the light source 4 shines on the middle of the chicken embryo 6 5 minutes later, adjust the height of the chicken embryo fixing frame 14 to 10 cm from the bottom of the beaker, detect the signal when the light source 4 shines on the bottom of the chicken embryo 6 and arrange and record the same group of three signals to obtain the first set of data. Change the wavelength of the light source 4 to 450nm, adjust the light source controller to the above three positions, and obtain the second set of data. When the wavelength is 560nm, change the light intensity to 15Lux, adjust the light source controller to the above three positions, and get the third set of data. By comparing the first set and the second set of data, the difference between the heartbeat signals of chicken embryos under different light wavelengths can be obtained. The stress response and regularity of chicken embryos to light wavelength can be obtained. By comparing the data of the first group and the third group, the difference of the heartbeat signal of the chicken embryo under different light intensities can be deduced, so that the stress law of the chicken embryo to the light intensity can be deduced.
上述具体实施方式用来解释说明本发明，而不是对本发明进行限制，在本发明的精神和权利要求的保护范围内，对本发明作出的任何修改和改变，都落入本发明的保护范围。 The above specific embodiments are used to explain the present invention, rather than to limit the present invention. Within the spirit of the present invention and the protection scope of the claims, any modification and change made to the present invention will fall into the protection scope of the present invention.
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| CN105191836B (en) * | 2015-08-21 | 2016-05-18 | 中国海洋大学 | A kind of quick seed choosing method based on scallop heartbeat index |
| CN105165660B (en) * | 2015-10-20 | 2017-08-25 | 山东大学齐鲁医院 | The monitoring device of live body poultry egg embryo movement of the foetus in hatching |
| RU2665117C2 (en) * | 2016-04-18 | 2018-08-28 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "РОССИЙСКИЙ ГОСУДАРСТВЕННЫЙ АГРАРНЫЙ ЗАОЧНЫЙ УНИВЕРСИТЕТ" | Method for recording the heart rate of a bird embryo without the shell destruction and the device for its implementation |
| CN108986066A (en) * | 2018-04-23 | 2018-12-11 | 中国农业科学院特产研究所 | A kind of detection method and system of chicken embryo |
| CN108990902B (en) * | 2018-09-03 | 2021-04-30 | 薄文海 | Feeding method for improving pigeon egg hatchability |
| CN109819941A (en) * | 2019-04-04 | 2019-05-31 | 中国农业大学 | A hatching illumination method that is beneficial to hatching of chicken embryos and the healthy growth of chicken flocks in the later period |
| CN113575467B (en) * | 2021-07-29 | 2022-09-09 | 福州木鸡郎智能科技有限公司 | Block chain algorithm-based egg positioning method suitable for free-range chickens |
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