CN104473975A - Preparation method of chlorella pyrenoidosa polyphenol - Google Patents
Preparation method of chlorella pyrenoidosa polyphenol Download PDFInfo
- Publication number
- CN104473975A CN104473975A CN201410747176.3A CN201410747176A CN104473975A CN 104473975 A CN104473975 A CN 104473975A CN 201410747176 A CN201410747176 A CN 201410747176A CN 104473975 A CN104473975 A CN 104473975A
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- China
- Prior art keywords
- polyphenol
- extract
- chlorella pyrenoidesa
- pulverize
- preparation
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- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 36
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 title abstract 3
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 title abstract 3
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 24
- 239000000284 extract Substances 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 230000001476 alcoholic effect Effects 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- -1 filter Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000000605 extraction Methods 0.000 description 6
- 241000195493 Cryptophyta Species 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- 241000195474 Sargassum Species 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000199920 Ectocarpus Species 0.000 description 1
- 241000227647 Fucus vesiculosus Species 0.000 description 1
- 241000199919 Phaeophyceae Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/05—Chlorophycota or chlorophyta (green algae), e.g. Chlorella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of chlorella pyrenoidosa polyphenol. The chlorella pyrenoidosa polyphenol is extracted by using the preparation method comprising the following steps of smashing, mixing, extracting and filtering. The preparation method has the advantages of high absorption rate, low cost, convenience for industrial production and the like.
Description
technical field:
the present invention relates to algae polyphenol extractive technique field, be specifically related to a kind of preparation method of Chlorella pyrenoidesa polyphenol.
Background technology
seeweed polyphenol is the polyphenol compound general name extracted from Sargassum, the unique physiological function had because of it and become the study hotspot in phenols field.Polyphenol is a large class formation difference, containing polyhydric compound general name, is extensively present in terrestrial plant.But along with modern analysis and isolation technics are applied to the exploitation of marine algae resource progressively deeply, seeweed polyphenol causes the concern of people day by day.From existing research, seeweed polyphenol content in the Sargassums such as Alga Sgrgassi Enerves, Fucus Vesiculosus, Ectocarpus is abundanter, but along with people use analysis of modernization, isolation technics is studied Sargassum deepens continuously, in increasing algae, extract polyphenol substance.
seeweed polyphenol is the different compound of a large class formation, and research finds that brown algae polyphenols has anti-tumor activity, antibacterial, antiviral activity, antioxidant activity, chemical defence activity, deodorant activities etc.Seeweed polyphenol is a kind of safety non-toxic, has the native chemical product of unique bioactive.China's marine algae resource enriches, and should utilize this resources advantage, and in conjunction with the biological characteristics of seeweed polyphenol uniqueness, research and development novel sea medicine and Biofunctional goods.At present in the extraction process of algae polyphenol, also have extraction step and be responsible for, efficiency is low waits deficiency.
summary of the invention:
object of the present invention provide a kind of simple, fast and efficient while in Chlorella pyrenoidesa, extract the preparation method of a kind of Chlorella pyrenoidesa polyphenol of polyphenol substance.
the object of the present invention is achieved like this.A preparation method for Chlorella pyrenoidesa polyphenol, is characterized in that: technical process is: pulverize, mix, extract, filter, concrete operation method follows these steps to successively:
(1) pulverize: gather Chlorella pyrenoidesa, clean, dry, pulverize 40 mesh sieves;
(2) mix: take Chlorella pyrenoidesa, then add the alcoholic solution that concentration is 10-80%, solid-liquid ratio is 20-100ml/g, stirs, obtained mixed liquor;
(3) extract: above-mentioned mixed liquor being placed in microwave power is that the microwave field of 500-1500W extracts, and microwave treatment time is 20-100s, obtainedly extract filtrate;
(4) filter: by above-mentioned filtrate obtained by freeze drying to Chlorella pyrenoidesa polyphenol extract.
beneficial effect of the present invention: the present invention adopts following steps to extract Chlorella pyrenoidesa polyphenol: pulverize, mixing, extract, filter, the present invention can reach that absorbance is high, cost is low, is convenient to the advantages such as suitability for industrialized production.
superiority of the present invention is:
(1) features simple and practical process, technological process safety;
(2) do not need main equipment instrument, production cost is low;
(3) realize simultaneously to the extraction of Chlorella pyrenoidesa raw material seeweed polyphenol, and be applicable to the process of a large amount of sample.
Detailed description of the invention
below in conjunction with specific embodiment, the present invention is further illustrated
embodiment 1:
a preparation method for Chlorella pyrenoidesa polyphenol, is characterized in that: technical process is: pulverize, mix, extract, filter, concrete operation method follows these steps to successively:
(1) pulverize: gather Chlorella pyrenoidesa, clean, dry, pulverize 40 mesh sieves;
(2) mix: take Chlorella pyrenoidesa, then add the alcoholic solution that concentration is 10-80%, solid-liquid ratio is 20-100ml/g, stirs, obtained mixed liquor;
(3) extract: above-mentioned mixed liquor being placed in microwave power is that the microwave field of 500-1500W extracts, and microwave treatment time is 20-100s, obtainedly extract filtrate;
(4) filter: by above-mentioned filtrate obtained by freeze drying to Chlorella pyrenoidesa polyphenol extract.
embodiment 2
seeweed polyphenol is extracted with following steps:
(1) gather Chlorella pyrenoidesa, clean, dry, pulverize 40 mesh sieves;
(2) take Chlorella pyrenoidesa, then add the alcoholic solution that concentration is 30%, solid-liquid ratio is 10ml/g, and agitation as appropriate is even.
(3) mixture of step 2 being placed in microwave power is that the microwave field of 800W extracts, and microwave treatment time is 20s.
(4) filter, filtrate lyophilization obtains polyphenol extract.
seeweed polyphenol extraction ratio is 2.51%.
embodiment 3
seeweed polyphenol is extracted with following steps:
(1) gather Chlorella pyrenoidesa, clean, dry, pulverize 40 mesh sieves;
(2) take Chlorella pyrenoidesa to close, then add the alcoholic solution that concentration is 20%, solid-liquid ratio is 20ml/g, and agitation as appropriate is even.
(3) mixture of step 2 being placed in microwave power is that the microwave field of 800W extracts, and microwave treatment time is 40s.
(4) filter, filtrate lyophilization obtains polyphenol extract.
seeweed polyphenol extraction ratio is 3.08%.
embodiment 4
seeweed polyphenol is extracted with following steps:
(1) gather Chlorella pyrenoidesa, clean, dry, pulverize 40 mesh sieves;
(2) take Chlorella pyrenoidesa, then add the alcoholic solution that concentration is 10%, solid-liquid ratio is 30ml/g, and agitation as appropriate is even.
(3) mixture of step 2 being placed in microwave power is that the microwave field of 800W extracts, and microwave treatment time is 60s.
(4) filter, filtrate lyophilization obtains polyphenol extract.
seeweed polyphenol extraction ratio is 2.11%.
Claims (1)
1. a preparation method for Chlorella pyrenoidesa polyphenol, is characterized in that: technical process is: pulverize, mix, extract, filter, concrete operation method follows these steps to successively:
(1) pulverize: gather Chlorella pyrenoidesa, clean, dry, pulverize 40 mesh sieves;
(2) mix: take Chlorella pyrenoidesa, then add the alcoholic solution that concentration is 10-80%, solid-liquid ratio is 20-100ml/g, stirs, obtained mixed liquor;
(3) extract: above-mentioned mixed liquor being placed in microwave power is that the microwave field of 500-1500W extracts, and microwave treatment time is 20-100s, obtainedly extract filtrate;
(4) filter: by above-mentioned filtrate obtained by freeze drying to Chlorella pyrenoidesa polyphenol extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410747176.3A CN104473975A (en) | 2014-12-10 | 2014-12-10 | Preparation method of chlorella pyrenoidosa polyphenol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410747176.3A CN104473975A (en) | 2014-12-10 | 2014-12-10 | Preparation method of chlorella pyrenoidosa polyphenol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104473975A true CN104473975A (en) | 2015-04-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410747176.3A Pending CN104473975A (en) | 2014-12-10 | 2014-12-10 | Preparation method of chlorella pyrenoidosa polyphenol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104473975A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115252885A (en) * | 2022-07-26 | 2022-11-01 | 湖北大学 | Hydrogel dressing crosslinked by multiple hydrogen bonds and preparation and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060024328A1 (en) * | 2004-07-30 | 2006-02-02 | David Pasco | Potent immunostimulatory extracts from microalgae |
| CN102166230A (en) * | 2011-04-14 | 2011-08-31 | 大连民族学院 | Microwave-assisted extraction method of seaweed polyphenol |
| CN102726808A (en) * | 2011-04-07 | 2012-10-17 | 浙江省海洋开发研究院 | Method for using alga polyphenol to prevent blackening of seawater shrimps |
-
2014
- 2014-12-10 CN CN201410747176.3A patent/CN104473975A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060024328A1 (en) * | 2004-07-30 | 2006-02-02 | David Pasco | Potent immunostimulatory extracts from microalgae |
| CN102726808A (en) * | 2011-04-07 | 2012-10-17 | 浙江省海洋开发研究院 | Method for using alga polyphenol to prevent blackening of seawater shrimps |
| CN102166230A (en) * | 2011-04-14 | 2011-08-31 | 大连民族学院 | Microwave-assisted extraction method of seaweed polyphenol |
Non-Patent Citations (2)
| Title |
|---|
| 欧阳小琨等: "微波辅助提取鼠尾藻多酚及抗氧化活性研究", 《中国民族民间医药》 * |
| 钟明杰等: "微波辅助提取紫菜多酚及抗氧化活性研究", 《食品科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115252885A (en) * | 2022-07-26 | 2022-11-01 | 湖北大学 | Hydrogel dressing crosslinked by multiple hydrogen bonds and preparation and application thereof |
| CN115252885B (en) * | 2022-07-26 | 2023-08-22 | 湖北大学 | Hydrogel dressing crosslinked by multiple hydrogen bonds and preparation and application thereof |
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Application publication date: 20150401 |