CN104839010B - A kind of selection of paddy rice High quality and diseases resistance is high temperature resistant two-line sterile line - Google Patents
A kind of selection of paddy rice High quality and diseases resistance is high temperature resistant two-line sterile line Download PDFInfo
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 191
- 235000009566 rice Nutrition 0.000 title claims abstract description 191
- 201000010099 disease Diseases 0.000 title claims abstract description 23
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 23
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 199
- 235000013339 cereals Nutrition 0.000 claims abstract description 88
- 238000004519 manufacturing process Methods 0.000 claims abstract description 51
- 230000035558 fertility Effects 0.000 claims abstract description 28
- 238000009401 outcrossing Methods 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 24
- 208000035240 Disease Resistance Diseases 0.000 claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims description 68
- 238000003306 harvesting Methods 0.000 claims description 22
- 230000006698 induction Effects 0.000 claims description 19
- 230000008569 process Effects 0.000 claims description 16
- 230000008929 regeneration Effects 0.000 claims description 15
- 238000011069 regeneration method Methods 0.000 claims description 15
- 241000607479 Yersinia pestis Species 0.000 claims description 10
- 239000010903 husk Substances 0.000 claims description 5
- 230000008775 paternal effect Effects 0.000 claims description 5
- 238000004088 simulation Methods 0.000 claims description 5
- 230000036512 infertility Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 1
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- 238000012214 genetic breeding Methods 0.000 abstract description 2
- 244000269722 Thea sinensis Species 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000002146 bilateral effect Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000010152 pollination Effects 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 208000015220 Febrile disease Diseases 0.000 description 1
- 240000002582 Oryza sativa Indica Group Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000007261 regionalization Effects 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
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Abstract
The present invention relates to a kind of selection of the high temperature resistant two-line sterile line germplasm of paddy rice High quality and diseases resistance, belongs to Crop Genetic Breeding technical field.The method is related to be polymerized the rice germplasm seed selection of the excellent economical character such as long grain, high head rice rate and the production of hybrid seeds is high temperature resistant, disease-resistant, it is bred as the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, the two-line sterile line of rice that disease resistance is good, Comprehensive Traits are excellent.Assembled using above-mentioned sterile line in a large number, it is expected to filter out high-quality, two-line hybrid rice Combination nova that is disease-resistant and being easy to the production of hybrid seeds.
Description
Technical field
Present invention relates particularly to a kind of selection of the high temperature resistant two-line sterile line germplasm of paddy rice High quality and diseases resistance, belongs to crop
Biotechnology in Genetic Breeding field.
Background technology
Paddy rice is one of China or even world's staple food crop, therefore improves rice yield and is particularly important, wherein,
It is the important breeding objective of hybrid rice that high yield and high quality is disease-resistant.Problem present in current rice heterosis utilization process is increasingly
Significantly, embody a concentrated expression of that per unit area yield increase rate is little, meter Zhi Yu resistance is big nothing breakthrough, hybrid rice seed production of hybrid seeds production risk
Deng series of problems, more and more frequent especially with extreme weather in recent years, greater risk is brought to Rice Production.As summer
It is rice production of hybrid seeds safety that low temperature has a strong impact on two；Hot conditions constrain Rice Hybrid Seed Production and percentage of seedgermination and hybridization
The setting percentage of rice large area production and rice quality；Bad weather conditions even induce breaking out for rice blast, result in portion
Divide underproduction total crop failure of paddy rice breeding of large area production etc., greatly production hidden danger is brought to the grain security of China.Therefore, seek
Excellent parental rice germ plasm resource is sought and formulate, is the important technical of Genetic and breeding in rice improvement.This patent is intended from two
It is that sterile line improvement is started with, by the excellent economical characters such as long grain, high head rice rate and the high temperature resistant, blast resisting of the production of hybrid seeds that are polymerized,
It is bred as the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, and disease resistance is good, Comprehensive Traits are excellent
Two-line sterile line, and then by a large amount of combos, it is desirable to obtain high yield, high-quality, disease-resistant, and be suitable for multi-environment production of hybrid seeds production
Two-line hybrid rice Combination nova.
Content of the invention
It is an object of the invention to provide the selection of paddy rice high-quality, disease-resistant, high temperature resistant two-line sterile line germplasm, utilizes
Above-mentioned sterile line is assembled strong advantage hybrid rice new combination in a large number, it is expected to filter out high yield, high-quality, disease-resistant, and seed production
The two-line hybrid rice Combination nova of low cost.
The method of the present invention is comprised the following steps：
1）With the high head rice rate kind-A of long grain type, column cap is high temperature resistant, easy production of hybrid seeds kind-B and high resistant to rice blast kind-
C is parent, makees cross combination FA/FB, FA/FC, respectively with FAB、FACRepresent；Plantation FAB、FAC, and hybridize FAB/FAC, obtain multiple
A generation is handed over, with FABCRepresent；Plantation FABCAnd harvest F‘ 2Seed；
2）By F‘ 2Seed sowing planting carries out induction morbidity, high resistant to rice blast product in rice blast grave illness area using induction kind
Planting C selects leaf pest onset grade in the rice shoot of 0-1 level for control, therefrom, transplants rice transplanting；Heading dough stage, carry out panicle blast mirror
Fixed, therefrom select high resistant to rice blast and seed is long grain individual plant, harvest F3Seed；
3）Spring sows F3, to ear the maturity period in summer high temperature, select plant annesl good, setting percentage is high, Comprehensive Traits are excellent
Good strain, harvests seed by strain, with long grain, high head rice rate kind A as control, is processed using small rice polisher,
Therefrom select rice matter outward appearance good, the high F of grain type length, head rice rate4For strain；
4）Repeat 2,3 authentication step, therefrom select rice matter outward appearance good, grain type length, head rice rate are high, the good F of disease resistance6
Strain；
5）With low-temperature-sensitive two-line sterile line as female parent, with the F being selected in6Strain is paternal hybrid, obtains hybrid Fs1Generation；Plantation
Fs1In generation, it is returned with corresponding male parent after heading, obtains BCs1Generation；
6）Plant corresponding BCs1In generation, mix respectively and receive BC1Fs1For seed；Plantation BC1Fs1More than 2000 plants of colony, Cong Zhongxuan
Select fertility good, seed elongated, stigma exposing ratio are high, what Comprehensive Traits were excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, autumn short-day
During low temperature, mixing harvests the selfed seed BC of sterile strain1Fs2；
7）By BC1Fs2Direct-seeding planting carries out induction morbidity, high resistant to rice blast product in rice blast grave illness area using induction kind
Planting C selects leaf pest onset grade in the rice shoot of 0-1 level for control, therefrom, transplants rice transplanting；Heading stage carries out panicle blast identification, from
Middle selection high resistant to rice blast and seed be long grain individual plant rice head, cut the root and stem of certain plants regeneration move to Hainan in Winter expand numerous, harvest Fs3Seed；With
Long grain, high head rice rate kind A are control, are processed using small rice polisher, therefrom select rice matter outward appearance good, grain type length,
The high strain of head rice rate；
8）Repeat 7 authentication step, until the long grain of acquisition grain, head rice rate height, outcrossing characteristic are good, stable fertility, resist
The paddy rice two that characteristic of disease is good, Comprehensive Traits are excellent is sterile strain Fs6.
9）Processed using the method for potted plant, growth cabinet simulation High Temperature Stress, humidity in climate box during process
For 60%, degree/day arranges as follows：7:00-9:00,37 DEG C；9:00-14:00,40 DEG C；14:00-16:00,37 DEG C；16:
00- next day 7:00,35 DEG C.With the corresponding CMS line of kind B as control, it is selected in sterile strain Fs by above-mentioned6In generation, presses strain
Transplanting per 2 plants of basin, is cut off before immigration and has opened grain husk flower in basin alms bowl respectively, and individual plant bagging, often covers 2 fringes；Ensureing abundance
On the premise of pollen amount, continuously pollinated 5 days using same restorer, measured in high-temperature process period downwards from fringe top during taking-up
The spike length degree that blooms, and listed record, process after terminating are placed in whole potted plants under natural conditions and manage.After seed maturity
Investigate setting percentage, clever rate and plumpness is split, select outcrossing seed-setting rate etc. easily to make better than the corresponding CMS line of kind B, high temperature
The strain that plants, cuts root and stem of certain plants regeneration and moves to the numerous acquisition Fs of Hainan in Winter expansion7Generation.
10) two it is sterile strain Fs using early season low temperature, High aititude and growth cabinet to be selected in7Generation sterile
Property and can numerous property identified, and with low-temperature-sensitive two-line sterile line as control, select fertility stability better than control, stagnation temperature
The low low-temperature-sensitive two of degree is sterile strain；Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, most
Grain long grain is obtained eventually, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, and disease resistance is good, Comprehensive Traits are excellent
Good two-line sterile line.
The high head rice rate kind of described long grain type is C10（Fu Haowei, high-quality mid-season super hybrid rice praise excellent 99 seed selection and
Its characteristic, Agriculture of Anhui science, 2004,32 (3):429）, column cap is high temperature resistant, the indica type maintainer of easy production of hybrid seeds rice variety
The II-32B or rich B of paddy（Xia Mingyuan, Qi Huaxiong etc., shadow of the Climatic regionalization to cross combination setting percentage that four sterile lines are prepared
Ring. hubei agricultural science, 2004. 02.21-22）；High resistant to rice blast kind is the extensively anti-13B or rich B of paddy（Indica Rice three be not
Educate be extensively anti-13A seed selection, Sanming City agricultural science and technology, the 3rd phase in 2003）, long grain type long-grained nonglutinous rice low-temperature-sensitive two-line sterile line Kingsoft
s-2（Fujian section mirror word [2004] the 116th）It is sterile gene donor for two.By hybridization, backcrossing；Naturally and manually combine
Authentication method, obtains that the long grain of grain, head rice rate are high, the production of hybrid seeds is high temperature resistant, and outcrossing characteristic is good, stable fertility, and disease resistance is good, comprehensive
The excellent two-line sterile line of proterties.
The outstanding advantages of the present invention
The long grain of grain that patent of the present invention is bred as, head rice rate is high, the production of hybrid seeds is high temperature resistant, and outcrossing characteristic is good, stable fertility, resists
The two-line sterile line that characteristic of disease is good, Comprehensive Traits are excellent has following advantages：(1) the long grain of sterile line seed, head rice rate are high, improve
The Appearance Quality of Paddy Rice of the indica type first generation of hybrid.（2）Sterile line outcrossing characteristic is good, the production of hybrid seeds is high temperature resistant, it is low to split clever rate, overcomes miscellaneous
Hand over the paddy rice production of hybrid seeds that the shortcoming of sterile line non-refractory low humidity is produced, hybrid seed yield and quality is improved, reduce production cost.（3）Change
Good two-line sterile line rice blast resistance, overcomes the current combination high yield and high quality of two-line hybrid rice mostly, but the deficiency of disease resistance difference,
Reduce because of the production hidden danger of disease underproduction total crop failure in production, promote high yield, high-quality, high anti-two-line hybrid rice Combination nova in production
Large-area applications.
Specific embodiment
Embodiment 1：
1）With high head rice rate kind C10 of long grain type（Represented with kind A）, column cap is high temperature resistant, easy production of hybrid seeds kind II-32B
The wide anti-13B of (being represented with kind B) and high resistant to rice blast kind（Represented with kind C）For parent, make cross combination FA/FB, FA/
FC, respectively with FAB、FACRepresent；Plantation FAB、FAC, and hybridize FAB/FAC, a generation of reestablishing diplomatic relations is obtained, with FABCRepresent；Plantation FABCAnd
Harvest F, 2Seed；
2）By F, 2Seed plants Hangzhoupro tea ground rice blast grave illness area on the Fujian Longyan, and using induction kind International Rice, No. 6 are entered
Row induction morbidity, extensively anti-13B is control to high resistant to rice blast kind, therefrom selects leaf pest onset grade in the rice shoot of 0-1 level, moves
Plant rice transplanting；Heading dough stage, panicle blast identification is carried out, therefrom select high resistant to rice blast and seed is long grain individual plant, harvest F3Kind
Son；
3）Spring sows F3, ripe in summer high temperature heading, select plant annesl good, setting percentage is high, Comprehensive Traits are excellent
Strain, by strain harvest seed, with long grain, high head rice rate kind C10 as control, be processed using small rice polisher,
Therefrom select rice matter outward appearance good, the high F of grain type length, head rice rate4For strain；
4）Repeat 2,3 authentication step, therefrom select rice matter outward appearance good, grain type length, head rice rate are high, the good F of disease resistance6
Strain；
5）With low-temperature-sensitive two-line sterile line Kingsoft s-2 as female parent, with the F being selected in6Strain is paternal hybrid, obtains hybrid Fs1
Generation；Plantation Fs1In generation, it is returned with corresponding male parent after heading, obtains BCs1Generation；
6）Plant corresponding BCs1In generation, mix respectively and receive BC1Fs1For seed；Plantation BC1Fs12100 plants of colony, therefrom selects to educate
Property good, seed elongated, stigma exposing ratio is high, and what Comprehensive Traits were excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, autumn short-day low temperature
When, mixing harvests the selfed seed BC of sterile strain1Fs2；
7）By BC1Fs2Seed plants Hangzhoupro tea ground rice blast grave illness area on the Fujian Longyan, using induction kind International Rice 6
Induction morbidity is carried out, extensively anti-13B is control to high resistant to rice blast kind, therefrom selects leaf pest onset grade in the rice shoot of 0-1 level, moves
Plant rice transplanting；Heading stage carries out panicle blast identification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves
Expand numerous, results Fs to Hainan in Winter3Seed；With long grain, high head rice rate kind C10 as control, carried out using small rice polisher
Processing, therefrom selects rice matter outward appearance good, the high strain of grain type length, head rice rate；
8）Repeat 7 authentication step, until the long grain of acquisition grain, head rice rate height, outcrossing characteristic are good, stable fertility, resist
The paddy rice two that characteristic of disease is good, Comprehensive Traits are excellent is sterile strain Fs6.
9）Processed using the method for potted plant, growth cabinet simulation High Temperature Stress, humidity in climate box during process
For 60%, degree/day arranges as follows：7:00-9:00,37 DEG C；9:00-14:00,40 DEG C；14:00-16:00,37 DEG C；16:
00- next day 7:00,35 DEG C.With CMS line II-32A as control, it is selected in sterile strain Fs by above-mentioned6In generation, is distinguished by strain
Transplanting per 2 plants of basin, is cut off before immigration and has opened grain husk flower in basin alms bowl, and individual plant bagging, often covers 2 fringes；Ensureing sufficient pollen
On the premise of amount, using the bright extensive 86 continuous pollination of restorer 5 days, measured from fringe top downwards during taking-up and open in high-temperature process period
Colored spike length degree, and listed record, process after terminating are placed in whole potted plants under natural conditions and manage.Examine after seed maturity
Examine setting percentage, clever rate and plumpness is split, outcrossing seed-setting rate etc. is selected better than II-32A, the strain of the easy production of hybrid seeds of high temperature, cut root and stem of certain plants regeneration
Move to Hainan in Winter and expand numerous acquisition Fs7Generation.
10) two it is sterile strain Fs using early season low temperature, High aititude and growth cabinet to be selected in7Generation sterile
Property and can numerous property identified, and with low-temperature-sensitive two-line sterile line as control, select fertility stability better than control, stagnation temperature
The low low-temperature-sensitive two of degree is sterile strain；Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, most
Grain long grain is obtained eventually, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, and disease resistance is good, Comprehensive Traits are excellent
Good two-line sterile line " Kingsoft s-3 ".
Advantage：The long grain of incubation, high head rice rate, two-line sterile line " Kingsoft s-3 " stable fertility of high temperature resistant, the easy production of hybrid seeds
（Critical-temperature≤23.5 DEG C）, the indica type long grain of seed, bran point purple, grain weigh 26.2g, length-width ratio 2.75, and head rice rate reaches
63.1%；The stigma exposing ratio of sterile line is 71.6%, and wherein bilateral exposing ratio is 43.7%, and production of hybrid seeds reproductive output is high；And high anti-rice
Seasonal febrile diseases, the first generation of hybrid yield that is prepared using the sterile line are high, and better resistance, rice are of fine quality, and hybrid seed yield is high, and hybrid vigour is obvious.
Embodiment 2：
1）With high head rice rate kind C10 of long grain type（Represented with kind A）, column cap is high temperature resistant, the easy production of hybrid seeds and disease-resistant variety
The wide anti-13B of the rich B of paddy (being represented with kind B) and high resistant to rice blast kind（Represented with kind C）For parent, make cross combination FA/
FB, FA/FC, respectively with FAB、FACRepresent；Plantation FAB、FAC, and hybridize FAB/FAC, a generation of reestablishing diplomatic relations is obtained, with FABCRepresent；Plantation
FABCAnd harvest F, 2Seed；
2）By F, 2Seed plants Hangzhoupro tea ground rice blast grave illness area on the Fujian Longyan, and using induction kind International Rice, No. 6 are entered
Row induction morbidity, extensively anti-13B is control to high resistant to rice blast kind, therefrom selects leaf pest onset grade in the rice shoot of 0-1 level, moves
Plant rice transplanting；Heading dough stage, panicle blast identification is carried out, therefrom select high resistant to rice blast and seed is long grain individual plant, harvest F3Kind
Son；
3）Spring sows F3, ripe in summer high temperature heading, select plant annesl good, setting percentage is high, Comprehensive Traits are excellent
Strain, by strain harvest seed, with long grain, high head rice rate kind C10 as control, be processed using small rice polisher,
Therefrom select rice matter outward appearance good, the high F of grain type length, head rice rate4Strain；
4）Repeat 2,3 authentication step, therefrom select rice matter outward appearance good, grain type length, head rice rate are high, the good F of disease resistance6
Strain；
5）With low-temperature-sensitive two-line sterile line Kingsoft s-2 as female parent, with the F being selected in6Strain is paternal hybrid, obtains hybrid Fs1
Generation；Plantation hybrid Fs1In generation, it is returned with corresponding male parent after heading, obtains BCs1Generation；
6）Plant corresponding BCs1In generation, mix respectively and receive BC1Fs1For seed；Plantation BC1Fs12000 plants of colony, therefrom selects to educate
Property be seed elongated, stigma exposing ratio is high, and what Comprehensive Traits were excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, autumn short-day low temperature
When, mixing harvests the selfed seed BC of sterile strain1Fs2；
7）By BC1Fs2Seed plants Hangzhoupro tea ground rice blast grave illness area on the Fujian Longyan, using induction kind International Rice 6
Induction morbidity is carried out, extensively anti-13B is control to high resistant to rice blast kind, therefrom selects leaf pest onset grade in the rice shoot of 0-1 level, moves
Plant rice transplanting；Heading stage carries out panicle blast identification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves
Expand numerous, results Fs to Hainan in Winter3Seed；With long grain, high head rice rate kind C10 as control, carried out using small rice polisher
Processing, therefrom selects rice matter outward appearance good, the high strain of grain type length, head rice rate；
8）Repeat 7 authentication step, until the long grain of acquisition grain, head rice rate height, outcrossing characteristic are good, stable fertility, resist
The paddy rice two that characteristic of disease is good, Comprehensive Traits are excellent is sterile strain Fs6.
9）Processed using the method for potted plant, growth cabinet simulation High Temperature Stress, humidity in climate box during process
For 60%, degree/day arranges as follows：7:00-9:00,37 DEG C；9:00-14:00,40 DEG C；14:00-16:00,37 DEG C；16:
00- next day 7:00,35 DEG C.With the rich A of CMS line paddy as control, it is selected in sterile strain Fs by above-mentioned6In generation, is inserted respectively by strain
Plant in basin alms bowl, per 2 plants of basin, cut off before immigration and grain husk flower has been opened, and individual plant bagging, often cover 2 fringes；Ensureing sufficient pollen amount
On the premise of, using the extensive 3301 continuous pollination in restorer Fujian 5 days, measured from fringe top downwards during taking-up and open in high-temperature process period
Colored spike length degree, and listed record, process after terminating are placed in whole potted plants under natural conditions and manage.Examine after seed maturity
Examine setting percentage, clever rate and plumpness is split, A rich better than paddy, the strains of the easy production of hybrid seeds of high temperature such as outcrossing seed-setting rate is selected, cut root and stem of certain plants regeneration and move
Expand numerous acquisition Fs to Hainan in Winter7Generation.
10) two it is sterile strain F using early season low temperature, High aititude and growth cabinet to be selected in7The sterility in generation
And can numerous property identified, and with low-temperature-sensitive two-line sterile line as control, select fertility stability better than control Kingsoft s-2,
The low low-temperature-sensitive two of critical-temperature is sterile strain；Repeat above-mentioned fertility, head rice rate and resistance and the identification of production of hybrid seeds outcrossing rate
Step, final obtain the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, and disease resistance is good, comprehensive
The excellent two-line sterile line of proterties " poly s ".
Advantage：The long grain of incubation, high head rice rate, two-line sterile line " poly s " stable fertility of high temperature resistant, the easy production of hybrid seeds
（Critical-temperature≤23.5 DEG C）, the sterile line high resistant to rice blast, the long grain of seed be indica type, bran point purple, and grain weighs 28.1g, length and width
Than 2.71, head rice rate 60.6%；Stigma exposing ratio is 68.2%, and wherein bilateral exposing ratio is 41.2%, and production of hybrid seeds reproductive output is high；
The two-line hybrid rice yield that is prepared using the sterile line is high, and better resistance, rice are of fine quality, and breeding cost is low, and production application potentiality are big.
Embodiment 3：
1）With high head rice rate water kind C10 of long grain type（Represented with kind A）, column cap is high temperature resistant, easy production of hybrid seeds maintainer II-
32B (being represented with kind B) and the rich B of high resistant to rice blast kind paddy（Represented with kind C）For parent, make cross combination FA/FB,
FA/FC, respectively with FAB、FACRepresent；Plantation FAB、FAC, and hybridize FAB/FAC, a generation of reestablishing diplomatic relations is obtained, with FABCRepresent；Plantation FABC
And harvest F, 2Seed；
2）By F, 2Seed plants Hangzhoupro tea ground rice blast grave illness area on the Fujian Longyan, is lured using induction kind " 946 "
Morbidity, the rich B of high resistant to rice blast kind paddy are control, therefrom select leaf pest onset grade in the rice shoot of 0-1 level, transplant rice transplanting；
Heading dough stage, panicle blast identification is carried out, therefrom select high resistant to rice blast and seed is long grain individual plant, harvest F3Seed；
3）Spring sows F3, ripe in summer high temperature heading, select plant annesl good, setting percentage is high, Comprehensive Traits are excellent
Strain, by strain harvest seed, with long grain, high head rice rate kind C10 as control, be processed using small rice polisher,
Therefrom select rice matter outward appearance good, the high F of grain type length, head rice rate4Strain；
4）Repeat 2,3 authentication step, therefrom select rice matter outward appearance good, grain type length, head rice rate are high, the good F of disease resistance6
Strain；
5）With low-temperature-sensitive two-line sterile line Kingsoft s-2 as female parent, with the F being selected in6Strain is paternal hybrid, obtains hybrid Fs1
Generation；Plantation hybrid Fs1In generation, it is returned with corresponding male parent after heading, obtains BCs1Generation；
6）Plant corresponding BCs1In generation, mix respectively and receive BC1Fs1For seed；Plantation BC1Fs12000 plants of colony, therefrom selects to educate
Property be seed elongated, stigma exposing ratio is high, and what Comprehensive Traits were excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, autumn short-day low temperature
When, mixing harvests the selfed seed BC of sterile strain1Fs2；
7）By BC1Fs2Seed sowing planting Hangzhoupro tea ground rice blast grave illness area on the Fujian Longyan, is entered using induction kind " 946 "
Row induction morbidity, the rich B of high resistant to rice blast kind paddy is control, selects leaf pest onset grade in the rice shoot of 0-1 level, transplants rice transplanting；
Heading stage carries out panicle blast identification, therefrom selects high resistant to rice blast and seed is long grain individual plant rice head, cuts root and stem of certain plants regeneration and moves to Hainan
Winter expands numerous, results Fs3Seed；With long grain, high head rice rate kind C10 as control, it is processed using small rice polisher, from
Middle selection rice matter outward appearance is good, the high strain of grain type length, head rice rate；
8）Repeat 7 authentication step, until the long grain of acquisition grain, head rice rate height, outcrossing characteristic are good, stable fertility, resist
The paddy rice two that characteristic of disease is good, Comprehensive Traits are excellent is sterile strain Fs6.
9）Processed using the method for potted plant, growth cabinet simulation High Temperature Stress, humidity in climate box during process
For 60%, degree/day arranges as follows：7:00-9:00,37 DEG C；9:00-14:00,40 DEG C；14:00-16:00,37 DEG C；16:
00- next day 7:00,35 DEG C.With CMS line II-32A as control, it is selected in sterile strain Fs by above-mentioned6In generation, is distinguished by strain
Transplanting per 2 plants of basin, is cut off before immigration and has opened grain husk flower in basin alms bowl, and individual plant bagging, often covers 2 fringes；Ensureing sufficient pollen
On the premise of amount, using the bright extensive 63 continuous pollination of restorer 5 days, measured from fringe top downwards during taking-up and open in high-temperature process period
Colored spike length degree, and listed record, process after terminating are placed in whole potted plants under natural conditions and manage.Examine after seed maturity
Examine setting percentage, clever rate and plumpness is split, outcrossing seed-setting rate etc. is selected better than II-32A, the strain of the easy production of hybrid seeds of high temperature, cut root and stem of certain plants regeneration
Move to Hainan in Winter and expand numerous acquisition Fs7Generation.
10) two it is sterile strain Fs using early season low temperature, High aititude and growth cabinet to be selected in7The sterility in generation
And can numerous property identified, and with low-temperature-sensitive two-line sterile line as control, select fertility stability better than control Kingsoft s-2,
The low low-temperature-sensitive two of critical-temperature is sterile strain；Repeat above-mentioned fertility, head rice rate and resistance and the identification of production of hybrid seeds outcrossing rate
Step, final obtain the long grain of grain, head rice rate is high, the production of hybrid seeds is high temperature resistant, outcrossing characteristic is good, stable fertility, and disease resistance is good, comprehensive
The excellent two-line sterile line of proterties " golden poly- s-2 ".
Advantage：The long grain of incubation, high head rice rate, two-line sterile line " the golden poly- s-2 " stable fertility of high temperature resistant, the easy production of hybrid seeds
（Critical-temperature≤23.5 DEG C）, the sterile line rice quality is excellent, high resistant to rice blast, and bran point purple, grain weigh 26.3g, length-width ratio
2.81, head rice rate reaches 65.5%；Stigma exposing ratio is 73.8%, and wherein bilateral exposing ratio is 46.1%, and production of hybrid seeds reproductive output is high；
The two-line hybrid rice yield that is prepared using the sterile line is high, and better resistance, head rice rate are high, and mouthfeel is good, and hybrid seed yield is high, cost
Low, it is expected to be bred as the two-line hybrid rice Combination nova of large scale application.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes that is done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (1)
1. the selection of the high temperature resistant disease-resistant two-line sterile line germplasm of a kind of paddy rice high-quality, it is characterised in that：Comprise the following steps：
1）With long grain type, high head rice rate rice varieties-A, column cap is high temperature resistant, easy production of hybrid seeds kind-B and high resistant to rice blast product
It is parent to plant-C, makees cross combination FA/FB, FA/FC, respectively with FAB、FACRepresent；Plantation FAB、FAC, and hybridize FAB/FAC, obtain
Reestablish diplomatic relations a generation, with FABCRepresent；Plantation FABCAnd harvest F, 2Seed；
2）By F, 2Seed is seeded in rice blast grave illness area, carries out induction morbidity using induction kind, and high resistant to rice blast kind C is right
According to therefrom selection leaf pest onset grade transplants rice transplanting in the rice shoot of 0-1 level；Heading-dough stage, carries out panicle blast identification, therefrom
Select high resistant to rice blast and seed is long grain individual plant, harvest F3Seed；
3）Spring sows F3, ear the maturity period in summer high temperature, select plant annesl good, the strain that setting percentage is high, Comprehensive Traits are excellent
System, harvests seed by strain, with long grain, high head rice rate kind A as control, is processed using small rice polisher, Cong Zhongxuan
Select that meter matter outward appearance is good, the high F of grain type length, head rice rate4Strain；
4）Repeat 2,3 authentication step, therefrom select rice matter outward appearance good, grain type length, head rice rate are high, the good F of disease resistance6Strain；
5）With low-temperature-sensitive two-line sterile line as female parent, with the F being selected in6Strain is paternal hybrid, obtains hybrid Fs1Generation；Plantation hybrid
Fs1In generation, it is returned with corresponding male parent after heading, obtains BCs1Generation；
6）Plant corresponding BCs1In generation, mix respectively and receive BC1Fs1For seed；Plantation BC1Fs1More than 2000 plants of colony, therefrom selects to educate
Property good, seed elongated, stigma exposing ratio is high, and what Comprehensive Traits were excellent two is sterile individual plant, cuts root and stem of certain plants regeneration, autumn short-day low temperature
When, mixing harvests the selfed seed BC of sterile strain1Fs2；
7）By BC1Fs2Seed is planted in rice blast grave illness area, carries out induction morbidity, high resistant to rice blast kind C using induction kind
For control, therefrom select leaf pest onset grade in the rice shoot of 0-1 level, transplant rice transplanting；Heading stage carries out panicle blast identification, Cong Zhongxuan
It is long grain individual plant rice head to select high resistant to rice blast and seed, cuts root and stem of certain plants regeneration and moves to Hainan in Winter and expand numerous, harvests BC1Fs3Seed；With length
Grain, high head rice rate kind A are control, are processed using small rice polisher, therefrom select rice matter outward appearance good, grain type length, whole
The high strain of polished rice rate；
8）Repeat 7 authentication step, until the long grain of acquisition grain, head rice rate height, outcrossing characteristic are good, stable fertility, disease resistance
The excellent paddy rice two of good, Comprehensive Traits is sterile strain BC1Fs6；
9）Processed using the method for potted plant, growth cabinet simulation High Temperature Stress, during process, in climate box, humidity is
60%, degree/day arranges as follows：7:00-9:00,37 DEG C；9:00-14:00,40 DEG C；14:00-16:00,37 DEG C；16:00-
Next day 7:00,35 DEG C, with the corresponding CMS line of kind B as control, it is selected in sterile strain BC by above-mentioned1Fs6In generation, is divided by strain
Other transplanting per 2 plants of basin, is cut off before immigration and has opened grain husk flower in basin alms bowl, and individual plant bagging, often covers 2 fringes；Ensureing sufficient flower
On the premise of powder amount, continuously pollinated 5 days using same restorer, measured from fringe top downwards during taking-up and open in high-temperature process period
Colored spike length degree, and listed record, process after terminating are placed in whole potted plants under natural conditions and manage, and examine after seed maturity
Examine setting percentage, clever rate and plumpness is split, select corresponding CMS line, the easy production of hybrid seeds of high temperature better than kind B such as outcrossing seed-setting rate
Strain, cut root and stem of certain plants regeneration and move to Hainan in Winter and expand numerous acquisition BC1Fs7Generation；
10) two it is sterile strain Fs using early season low temperature, High aititude and growth cabinet to be selected in7The sterility in generation and can
Numerous property is identified, and with low-temperature-sensitive two-line sterile line as control, selects fertility stability better than control, and critical-temperature is low
Low-temperature-sensitive two is sterile strain；Repeat above-mentioned fertility, head rice rate and resistance and production of hybrid seeds outcrossing rate authentication step, final acquisition
The long grain of grain, head rice rate are high, the production of hybrid seeds is high temperature resistant, and outcrossing characteristic is good, stable fertility, disease resistance is good, Comprehensive Traits are excellent two
It is sterile line；
The high head rice rate kind of described long grain type praises excellent 99 male parent C10 for new hybrid rice varieties, column cap is high temperature resistant, easy system
Plant indica type maintainer II-32B of rice variety or the rich B of paddy；High resistant to rice blast kind is the wide anti-13B or rich B of paddy, and low-temperature-sensitive two is
Sterile line is Kingsoft s-2.
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| CN105475121B (en) * | 2016-01-29 | 2018-10-26 | 重庆市农业科学院 | A kind of selection of high temperature resistant disease resisting rice two-line sterile line |
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| CN111955341A (en) * | 2020-08-10 | 2020-11-20 | 江苏沿海地区农业科学研究所 | Efficient breeding method of heat-resistant rice |
| CN112753570A (en) * | 2021-02-19 | 2021-05-07 | 湖南袁创超级稻技术有限公司 | Breeding method of short-grain rice sterile line and simple seed production technology application |
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| US8288635B2 (en) * | 2009-09-22 | 2012-10-16 | Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar templeton |
| CN103229712B (en) * | 2013-05-06 | 2014-02-19 | 福建农林大学 | A kind of breeding method of multi-resistance and cold-tolerant two-line sterile line |
| CN103891605B (en) * | 2014-04-04 | 2015-06-03 | 福建农林大学 | Breeding method for disease-resistant functional rice two-line sterile line |
| CN104137765B (en) * | 2014-07-14 | 2015-11-18 | 福建农林大学 | The selection of high resistance to cold and diseases long fringe neck two-line sterile line |
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