CN104894040B - Salmonella in Food one-step method selective enrichment medium and preparation method thereof - Google Patents
Salmonella in Food one-step method selective enrichment medium and preparation method thereof Download PDFInfo
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- CN104894040B CN104894040B CN201510384409.2A CN201510384409A CN104894040B CN 104894040 B CN104894040 B CN 104894040B CN 201510384409 A CN201510384409 A CN 201510384409A CN 104894040 B CN104894040 B CN 104894040B
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- 241000607142 Salmonella Species 0.000 title claims abstract description 29
- 235000013305 food Nutrition 0.000 title claims description 12
- 238000002360 preparation method Methods 0.000 title claims description 7
- 238000000034 method Methods 0.000 title claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 24
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims abstract description 20
- 108010080698 Peptones Proteins 0.000 claims abstract description 14
- XUFXOAAUWZOOIT-SXARVLRPSA-N (2R,3R,4R,5S,6R)-5-[[(2R,3R,4R,5S,6R)-5-[[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)-1-cyclohex-2-enyl]amino]-2-oxanyl]oxy]-3,4-dihydroxy-6-(hydroxymethyl)-2-oxanyl]oxy]-6-(hydroxymethyl)oxane-2,3,4-triol Chemical compound O([C@H]1O[C@H](CO)[C@H]([C@@H]([C@H]1O)O)O[C@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O)N[C@@H]1[C@@H]([C@@H](O)[C@H](O)C(CO)=C1)O)C)[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O XUFXOAAUWZOOIT-SXARVLRPSA-N 0.000 claims abstract description 13
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 13
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 13
- 229930195725 Mannitol Natural products 0.000 claims abstract description 13
- 229960002632 acarbose Drugs 0.000 claims abstract description 13
- XUFXOAAUWZOOIT-UHFFFAOYSA-N acarviostatin I01 Natural products OC1C(O)C(NC2C(C(O)C(O)C(CO)=C2)O)C(C)OC1OC(C(C1O)O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O XUFXOAAUWZOOIT-UHFFFAOYSA-N 0.000 claims abstract description 13
- 235000015278 beef Nutrition 0.000 claims abstract description 13
- 229940099352 cholate Drugs 0.000 claims abstract description 13
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 claims abstract description 13
- 239000004467 fishmeal Substances 0.000 claims abstract description 13
- 239000000594 mannitol Substances 0.000 claims abstract description 13
- 235000010355 mannitol Nutrition 0.000 claims abstract description 13
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 13
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 13
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 13
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims abstract description 13
- 235000019345 sodium thiosulphate Nutrition 0.000 claims abstract description 13
- 239000012153 distilled water Substances 0.000 claims abstract description 12
- 239000011780 sodium chloride Substances 0.000 claims abstract description 12
- 239000012137 tryptone Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 11
- 229940054269 sodium pyruvate Drugs 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 150000002576 ketones Chemical class 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 2
- 229940083542 sodium Drugs 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 3
- 235000014106 fortified food Nutrition 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 16
- 239000002609 medium Substances 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 8
- 238000001514 detection method Methods 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 239000008187 granular material Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the selective enrichment medium of salmonella, it is related to a kind of few for quantity in enriched food and to being damaged cell selective enrichment medium having repairing effect.It is characterized in that, in terms of mass fraction, composition of raw materials composition are as follows: 2.5-7.5 parts of tryptone, 2.5-7.5 parts of fish meal protein peptone, 2-8 parts of beef extract, 2.5-7.5 parts of sodium chloride, 1-2 parts of disodium hydrogen phosphate, 6-12 parts of potassium dihydrogen phosphate, 1-3 parts of mannitol, 5-15 parts of Sodium Pyruvate, 0.2-1 parts of acarbose, No. 5 1.25-5 parts of cholate, 10-30 parts of sodium thiosulfate, 1000 parts of distilled water, pH value is 7.2 ~ 7.5.
Description
Technical field
The invention belongs to the selective enrichment mediums of salmonella, are related to a kind of few for quantity in enriched food
And to being damaged cell selective enrichment medium having repairing effect.
Technical background
Salmonella (Salmonella spp.) it is the important pathogenic bacteria that can cause food origin disease in enterobacteriaceae.I
It is by salmonellal that state's food posioning event, which has 70% ~ 80%, and salmonellal food poisoning usually accounts for
According to the first place of food posioning.
Whether standard chemical examines association (AOAC), International Organization for Standardization or national standard GB4789.4-
The detection of Salmonella in Food will carry out salmonella in sample by preceding increasing bacterium and increasing two steps of bacterium in 2010
Then enrichment culture carries out subsequent separation, Physiology and biochemistry and serological Identification again, this usually to take a substantial amount of time and essence
Power, different increasing bacterium methods also may all will affect testing result, and influenced salmonella by factors such as hot-cool environments and led to
Often in sub- lethal state, so often will lead to increase bacterium and fail and missing inspection.Current enriched medium has very much, mainly
To the compound increasing bacterium of a variety of pathogenic microorganisms, different pathogenic bacteria grow under unified environment mutually to be inhibited, and be directed to food at present
In single pathogenic bacteria a step enriched medium it is seldom, so there is weight to the research of pathogenic bacteria enriched medium specific in food
Want meaning.
Summary of the invention
The purpose of the present invention is to provide it is a kind of can in fast culture food quantity it is few and by the sramana of sublethal damage
Salmonella increases the culture medium of bacterium, the detection suitable for subsequent quantitative fluorescent PCR.
The purpose of the present invention is achieved through the following technical solutions:
A kind of quick enriched medium of Salmonella in Food, in terms of mass fraction, formula composition are as follows: tryptone
2.5-7.5 parts, 2.5-7.5 parts of fish meal protein peptone, 2-8 parts of beef extract, 2.5-7.5 parts of sodium chloride, 1-2 parts of disodium hydrogen phosphate,
6-12 parts of potassium dihydrogen phosphate, 1-3 parts of mannitol, 5-15 parts of Sodium Pyruvate, 0.2-1 parts of acarbose, No. 5 1.25-5 parts of cholate,
10-30 parts of sodium thiosulfate, 1000 parts of distilled water, pH value is 7.2 ~ 7.5.
Optimization formula of the invention is, in terms of mass fraction: 5 parts of tryptone, 5 parts of fish meal protein peptone, and beef extract 4
Part, 5 parts of sodium chloride, 1.5 parts of disodium hydrogen phosphate, 9 parts of potassium dihydrogen phosphate, 2 parts of mannitol, 8 parts of Sodium Pyruvate, acarbose 0.3
Part, No. 5 2.5 parts of cholate, 25 parts of sodium thiosulfate, 1000 parts of distilled water, pH value 7.2.
A further object of the present invention is to provide salmonella enriched medium preparation method, it is by following steps system
At:
(1) proportionally by tryptone, fish meal protein peptone, beef extract, sodium chloride, disodium hydrogen phosphate, 12 hydrations
Potassium dihydrogen phosphate, mannitol, Sodium Pyruvate, acarbose, cholate 5, it is molten that sodium thiosulfate adds to agitating and heating in distilled water
Solution.
(2) after being cooled to room temperature, adjusting pH with 1 mol/L NaOH is 7.2 ~ 7.5.
(3) 121 DEG C of 15 min of high pressure sterilization.
The present invention is basic culture medium with BPW, LB, NB, TSB, and wherein suitable component carries out mixture for selection, obtains basis
Culture medium.Peptone, fish meal protein peptone, beef extract provide required carbon source for bacterial growth, nitrogen source, inorganic salts, growth because
The nutritional ingredients such as son.Disodium hydrogen phosphate and potassium dihydrogen phosphate can keep pH in culture medium to keep stablizing as buffer reagent.Again
Mannitol is selected, Sodium Pyruvate is as growth promoter, and wherein Sodium Pyruvate has repairing damage bacterium and promotes Salmonella
The effect of bacterium growth, acarbose, cholate 5, sodium thiosulfate is as varied bacteria growing inhibitor.
Compared with the existing technology, the invention has the following advantages that
The present invention increases two steps of bacterium and selective enrichment before capable of merging Salmonella in Food detection, will increase the contracting of bacterium time
Be short to 12-16 h, and salmonella while proliferation other microorganisms by a degree of inhibition.
The present invention according to formula and preparation method prepare enriched medium can directly salmonella be separately cultured and
Identification, also can be directly used for the detection of subsequent quantitative fluorescent PCR.
Specific embodiment
To be best understood from the present invention, the present invention will be described in further detail with reference to the following examples, but of the invention
Claimed range is not limited to the range of embodiment expression
Embodiment 1
Weigh 2.5 g of tryptone, 7.5 g of fish meal protein peptone, 2 g of beef extract, 7.5 g of sodium chloride, phosphoric acid hydrogen two
1 g of sodium, 12 g of potassium dihydrogen phosphate, 1 g of mannitol, 15 g of Sodium Pyruvate, 0.2 g of acarbose, No. 55 g of cholate, sulphur
10 g of sodium thiosulfate, 1000 mL of distilled water.
By tryptone, fish meal protein peptone, beef extract, sodium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, mannitol, third
Ketone acid sodium, acarbose, No. 3 cholate, sodium thiosulfate, distilled water stirring and dissolving, with 1 mol/L NaOH tune pH to 7.2,121
DEG C 15 min of high pressure sterilization is saved under room temperature.
It is 10 by cell concentration9 The salmonella culture solution dilution 10 of CFU/L6Times, then take 1 mL to access 99 mL preparation
Good is used in salmonella enriched medium, makes salmonella cell concentration 10 in culture medium1CFU/L, at 37 DEG C, 200
The h of r/min shaking table culture 12 and 16, the enrichment liquid 50uL after taking Zengjing Granule are applied to TTB selection after carrying out appropriate gradient dilution
Property culture medium flat plate culture 24-48 h after carry out bacterium colony counting.It the results are shown in Table 1.
As known from Table 1, by the increasing bacterium of 12-16 h, the cell concentration of salmonella increases to 10 by 10 CFU/mL8 CFU/
mL~109 CFU/mL meets later period PCR detection needs.
Embodiment 2
Weigh 7.5 g of tryptone, 2.5 g of fish meal protein peptone, 8 g of beef extract, 2.5 g of sodium chloride, phosphoric acid hydrogen
2 g of disodium, 6 g of potassium dihydrogen phosphate, 3 g of mannitol, 5 g of Sodium Pyruvate, 1 g of acarbose, No. 5 1.25 g of cholate,
30 g of sodium thiosulfate, 1000 parts of distilled water.
By tryptone, fish meal protein peptone, beef extract, sodium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, mannitol, third
Ketone acid sodium, acarbose, No. 3 cholate, sodium thiosulfate, distilled water stirring and dissolving, 1 mol/L NaOH tune pH to 7.5,121 DEG C
15 min of high pressure sterilization is saved under room temperature.
It is 10 by cell concentration9The salmonella culture solution dilution 10 of CFU/L6Times, then take 1 mL to access 99 mL preparation
Good is used in salmonella enriched medium, makes salmonella cell concentration 10 in culture medium1CFU/L, at 37 DEG C, 200
The h of r/min shaking table culture 12 and 16, the enrichment liquid 50uL after taking Zengjing Granule are applied to TTB agar after carrying out appropriate gradient dilution
Bacterium colony counting is carried out after plate culture 24-48 h.It the results are shown in Table 2.
As known from Table 1, by the increasing bacterium of 12-16 h, the cell concentration of salmonella increases to 10 by 10 CFU/mL8 CFU/
mL~109 CFU/mL meets later period PCR detection needs.
Embodiment 3
Weigh 5 g of tryptone, 5 g of fish meal protein peptone, 4 g of beef extract, 5 g of sodium chloride, disodium hydrogen phosphate
1.5 g, 9 g of potassium dihydrogen phosphate, 2 g of mannitol, 10 g of Sodium Pyruvate, acarbose 0.6 g, No. 3 2.5 g of cholate, sulphur
25 g of sodium thiosulfate, 1000 mL of distilled water.
By tryptone, fish meal protein peptone, beef extract, sodium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, mannitol, third
Ketone acid sodium, acarbose, No. 3 cholate, sodium thiosulfate, distilled water stirring and dissolving, 1mol/L NaOH tune pH to 7.3,121 DEG C
15 min of high pressure sterilization is saved under room temperature.
It is 10 by cell concentration9The salmonella culture solution dilution 10 of CFU/L6Times, then take 1 mL to access 99 mL preparation
Good is used in salmonella enriched medium, makes salmonella cell concentration 10 in culture medium1 CFU/L, at 37 DEG C, 200 r/
The h of min shaking table culture 12 and 16, enrichment liquid 50uL after taking Zengjing Granule carry out being applied to TTB agar after appropriate gradient dilution flat
Bacterium colony counting is carried out after plate culture 24-48 h.It the results are shown in Table 3
Claims (1)
1. being used for Salmonella in Food one-step method selective enrichment medium, which is characterized in that in terms of mass fraction, raw material
Formula composition are as follows: 2.5-7.5 parts of tryptone, 2.5-7.5 parts of fish meal protein peptone, 2-8 parts of beef extract, sodium chloride 2.5-7.5
Part, 1-2 parts of disodium hydrogen phosphate, 6-12 parts of potassium dihydrogen phosphate, 1-3 parts of mannitol, 5-15 parts of Sodium Pyruvate, acarbose 0.2-1
Part, No. 5 1.25-5 parts of cholate, 10-30 parts of sodium thiosulfate, 1000 parts of distilled water, pH value is 7.2~7.5；With mass fraction
Meter；For the preparation method of salmonella one-step method selective enrichment medium, include the following steps:
(1) by tryptone, fish meal protein peptone, beef extract, sodium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, mannitol, third
Ketone acid sodium, acarbose, cholate 5, sodium thiosulfate is added in distilled water, micro- heating hydrotropy；
(2) after being cooled to room temperature, adjusting pH with 1mol/L NaOH is 7.2~7.5；
(3) 121 DEG C of high pressure sterilization 15min.
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| CN110438051A (en) * | 2019-08-29 | 2019-11-12 | 海南省妇幼保健院 | A method of producing the Salmonella strains of high yield |
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| CN106896225A (en) * | 2017-04-28 | 2017-06-27 | 河南艾能生物科技有限公司 | A kind of method for quick of salmonella |
| CN111575215B (en) * | 2020-06-05 | 2022-04-22 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Heat damage salmonella culture medium and preparation method thereof |
| CN113416765B (en) * | 2021-08-12 | 2022-08-16 | 合肥工业大学 | Detection method of Cronobacter sakazakii in infant formula milk powder |
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| 1种选择性富集沙门氏菌、志贺氏菌、金黄色葡萄球菌和副溶血性弧菌共增菌培养基SSSV研究;翁思聪等;《中国食品学报》;20130131;第13卷(第1期);19-28 |
| Evaluation of culture media for selective enrichment and isolation of Salmonella in seafood.;Rakesh Kumar等;《Journal of AOAC International》;20101231;第93卷(第5期);387-393 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438051A (en) * | 2019-08-29 | 2019-11-12 | 海南省妇幼保健院 | A method of producing the Salmonella strains of high yield |
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