CN105062885B - A kind of family expenses prepare the algae juice machine of haematococcus pluvialis juice - Google Patents

Abstract

The invention discloses a home-use algae juice machine for preparing Haematococcus pluvialis juice, which comprises a closed device body and is divided into four modules: a sterilization module, a primary cultivation module, a secondary cultivation module and a drinking module, each of which is There are adjustable heating devices at the bottom of the modules, and the modules are separated by temperature insulation boards. The sterilizing module of the algae juice machine is connected to the water inlet device through a PVC hose, and the side wall of the sterilizing module is equipped with a nutrient storage and adding device; The ball control valve closes the water channel; the algae cells in the green stage are cultivated in the primary cultivation module, and the algae cells are transformed from green to red in the secondary cultivation module; the red algae liquid in the secondary cultivation module flows into the drinking module by turning on the drinking switch Heating pot; the heating pot can be used for drinking after being heated by the heating device of the drinking module. The algae juice machine of the present invention has simple structure and convenient operation, greatly increasing operability and convenience.

Description

A home-use algae juice machine for preparing Haematococcus pluvialis juice

technical field

The invention relates to a household algae juice machine for preparing Haematococcus pluvialls juice, which belongs to the technical field of small kitchen appliances.

Background technique

Microalgae can effectively use light energy, CO ₂ and inorganic salts to synthesize proteins, fats, carbohydrates and a variety of high value-added bioactive substances, and can produce health food, food additives, feed, biological fertilizers, cosmetics through microalgae cultivation and other natural products.

Haematococuus pluvialis is a single-celled freshwater green alga that belongs to Chlorophyta, Chlorophyceae, Volvox, Haematococuaceae, and Haematococcus genus in taxonomy. Under environmental stress conditions, it can rapidly synthesize a red ketone carotenoid astaxanthin, which is recognized as the best organism for producing natural astaxanthin in nature, and its accumulation can reach up to 4% of dry cell weight. Astaxanthin can be used as a feed additive in aquaculture (such as salmon) and poultry farming to prove that it is safe and edible. More importantly, astaxanthin has the strongest anti-fat and anti-oxidation ability, which is 10 times higher than β-carotene and 100 times higher than vitamin E. It is also known as "super vitamin E". It has been used in nutrition and health care, Pharmaceutical and cosmetic fields. Especially in European and American countries, natural astaxanthin health food is very popular among the people.

Haematococcus pluvialis has special biological properties, and there are mainly two different cell types in its life cycle, green swimming cells that undergo vegetative growth and red immobile cells that accumulate astaxanthin. Under favorable growth conditions, the cells grow vigorously and exist as green swimming cells, which contain a very small amount of astaxanthin; while under unfavorable conditions, the cells are stressed, the growth tends to be slow, and the tails of the swimming cells fall off. Loss of motor ability gradually tends to static state from swimming state, cell volume increases, transforms into chlamydospores, accumulates a large amount of astaxanthin at the same time, and cells turn from green to red.

At present, the domestic astaxanthin product market is a health food approved by the State Food and Drug Administration, mainly imported health food. Haematococcus pluvialis is mainly used in large-scale commercial cultivation, and the extracted natural astaxanthin is mostly used as an additive, health care product and cosmetic raw material for export. There is a large demand for astaxanthin in domestic and foreign markets, and the market prospect is promising. However, the source of astaxanthin products in the market is unknown, the market is chaotic, the price is high, and it is not easy to obtain. The unique biological characteristics of algal species and high requirements for cultivation conditions all restrict Haematococcus pluvialis from becoming a daily health food for Chinese people.

Contents of the invention

The technical problem to be solved by the present invention is to provide a home-use algae juice machine for preparing Haematococcus pluvialis juice in view of the deficiencies in the prior art. Algae, the algae juice machine is simple in structure and easy to operate, which greatly increases the operability of small-scale cultivation of Haematococcus pluvialis at home. The sterile product is algae juice rich in astaxanthin, which is safe and safe to use.

In order to achieve the above object, the present invention adopts the following technical solutions:

An algae juice machine for preparing Haematococcus pluvialis juice for home use. The main body of the algae juice machine is in the shape of a cuboid, including a sterilization module, a primary cultivation module, a secondary cultivation module and a drinking module, and each module is separated by a heat insulation board ; And the sterilization module, the first-level cultivation module, the second-level cultivation module, and the drinking module each have a heater, the heating temperature of the heater is set by an automatic program, and each module has a temperature sensor to detect and keep each module running at a specific temperature ; A heating pot is placed inside the drinking module.

Further, the heater is a heating plate located at the bottom of the sterilization module, the primary cultivation module, the secondary cultivation module and the drinking module.

Further, the algae juice machine is also equipped with a main control board, which displays the real-time temperatures of the sterilization module, the primary cultivation module, the secondary cultivation module, and the drinking module, and two touch buttons to control the entire System operation and shutdown. The whole system needs to add nutrient salt and water again after being quoted once, so it is a semi-continuous culture and fully automatic control.

Further, the sterilizing module is located on the upper part of the main body of the algae juice machine, and the top has a round hole as a water inlet; the primary cultivation module and the secondary cultivation module are all located below the sterilization module, and the primary cultivation module The volume of the module is larger than that of the secondary cultivation module; the drinking module is located below the secondary cultivation module and adjacent to the primary cultivation module.

Further, a small water bucket is placed on the water inlet, and the small water bucket is connected to an external water supply device through a PVC hose, and the external water supply device is ordinary bottled mineral water; the PVC hose can be installed near the bottled mineral water. valve to prevent suck-back when changing the water supply bucket.

Further, a reagent storage box is installed on the side of the sterilization module, and the reagent storage box contains nutrients needed for the growth of Haematococcus pluvialis; the reagent storage box includes a pressure button, a small internal reagent temporary storage box, LED ultraviolet light, press the pressure pop-up button to pop out the internal small reagent storage box, take back the kit after adding the reagent, turn on the ultraviolet light for sterilization, dump the kit after sterilization and pour the reagent into the sterilization module.

Further, the bottom of the sterilization module has a water flow control valve, the side wall of the primary cultivation module has a water level sensor, the heater in the sterilization module is a water supply timing heating device, and the water enters the sterilization module from the water inlet. Start the heating device to heat to boiling and then turn off the heating device. When the temperature monitor cools to 20°C and the water level sensor detects that the water level has dropped, the flow control valve is opened, and the sterilized constant temperature water enters the primary cultivation module for Haematococcus pluvialis cultivation.

Further, there is a float control valve inside the secondary cultivation module, including a lever and a floating ball. There is a floating ball control valve on the side heat shield shared by the primary cultivation module and the secondary cultivation module, which changes with the water level. Pull the round PC sheet to adjust the opening and closing of the round hole channel. The channel is a water channel. The floating ball and the lever are connected through polypropylene PP filaments, and the lever and the channel are connected through PC short rods; when the water level of the secondary culture module drops, The floating ball descends as the water level drops, and the lever is pulled to open the water channel, and the pressure difference generated due to the water level difference makes the green swimming cells in the primary culture module enter the secondary culture module.

Further, the drinking module and the bottom of the secondary sterilization module have channels that can be opened and closed, and the opening and closing are controlled by the drinking switch attached to the channel.

Further, the algae juice machine as a whole is made of transparent polycarbonate (PC) material and aluminum alloy material to make a closed cultivation space, which can effectively avoid algae pollution during the cultivation process; the primary cultivation module and the secondary cultivation module The material is a transparent polycarbonate material, and the transparent material greatly increases the light surface area volume ratio of the algae liquid of the primary cultivation module, improves the light utilization rate of the reactor, and improves the culture density of the reactor; the heating pot Made of clear polycarbonate material.

Further, the specifications of the algae juice machine are: 46cm in length, 23cm in width, and 46cm in height; the clapboard is close to the fuselage to form a closed space; the water injection depth is 25cm high for the primary cultivation module, and the secondary cultivation module is 25cm high. 18cm, the lower part of the entrance of the nutrient supplementation device is 25cm higher than the base, and the thickness of the fuselage partition is uniform, all of which are 3mm.

When using the algae juice machine of the present invention, the method comprises the following steps:

(1) Water injection and inoculation: place a small bucket on the water inlet of the fuselage, connect the small bucket to the external water supply device with a PVC hose, turn on the system and open the water supply device to inject water into the equipment, and the heating plate is distributed in the sterilization module for heating The mineral water is boiled to achieve the sterilization effect; the sterilization is carried out in the sterilization module and is detected by the temperature sensor when it is cooled to 20°C, and when the water level sensor detects that the water level has dropped, the flow control valve opens and the sterilization is kept at a constant temperature. Water enters the primary cultivation module to cultivate Haematococcus pluvialis;

(2) Carry out initial inoculation in the primary culture module, so that the cell inoculation density is 5×10 ⁴ -10×10 ⁴ cells/ml;

(3) Add nutrient salts: Add food-grade nutrient salts for Haematococcus pluvialis into the reagent storage box, press the pressure button, the internal reagent temporary storage box pops up, pour in nutrient salts, and the internal reagent temporary storage box returns to the body. The LED ultraviolet lamp starts to work to sterilize the air and nutrient salts in the kit by radiation; then the internal reagent temporary storage box is poured inward, and the nutrient salts enter the sterilization module and are mixed evenly under the entry of sterilized water. Add nutrient salts once after pouring out the algae liquid for each drinking. The nutrient salts are professional aseptic configurations and quantitative configurations;

(4) Primary cultivation: The heating plate distributed at the bottom of the primary cultivation module is heated, the temperature reaches 20°C and is maintained at 20°C, and the biomass is expanded at a constant temperature of 20°C for 5-8 days. Haematococcus pluvialis is a green swimming cell , when the height reaches 25cm, it flows into the secondary cultivation module;

(5) Secondary cultivation: the heating plate distributed at the bottom of the secondary cultivation module is heated, the temperature reaches 40°C and is maintained at 40°C, cultivated at a constant temperature of 4°C for 4-5 days, from green to red, and a large amount of astaxanthin is accumulated;

(6) Drinking: When the cell liquid turns red, turn on the drinking switch, the algae liquid from the secondary cultivation module flows out, the main control board touches the heating button, heats the algae liquid to boiling, cools and seasons and then drinks.

The algae juice machine of the present invention is suitable for small household cultivation of Haematococcus pluvialis rich in astaxanthin, and can achieve automatic, aseptic and continuous cultivation; The operability of cultivating Haematococcus pluvialis, the use of semi-continuous cultivation has greatly improved the cultivation efficiency, the automatic control saves time and cost, increases the operability and convenience, and the sterile product astaxanthin-rich algae juice is safe to drink , safety, health and nutrition. Improve the quality of life and health of the public.

Description of drawings

Fig. 1: the overall structure schematic diagram of algae juice machine of the present invention;

Figure 2: Schematic diagram of the structure of the reagent storage box for the algae juice machine of the present invention;

Fig. 3: the sectional view of algae juice machine of the present invention;

Figure 4: Schematic diagram of the structure of the float control valve of the algae juice machine of the present invention;

Figure 5: Schematic diagram of the water supply device for the algae juice machine of the present invention;

Figure 6: Schematic diagram of the main control board of the algae juice machine of the present invention;

Among them: 1. Sterilization module, 2. Primary cultivation module, 3. Secondary cultivation module, 4. Drinking module, 5. Water inlet, 6. Reagent storage box, 7. Heating plate, 8. Main control board, 9 , drinking switch, 10, float control valve, 11, heating pot, 12, pressure button, 13, LED ultraviolet light, 14, reagent temporary storage box, 15, temperature sensor, 16, water level sensor, 17, flow control valve, 18, water channel, 19, lever, 20, floating ball, 21, small bucket.

detailed description

The structure and operation mode of the household preparation Haematococcus pluvialls juice machine of the present invention will be described in detail below in conjunction with the accompanying drawings.

A household algae juice machine for preparing Haematococcus pluvialis juice, as shown in Figure 1, includes a sterilization module 1, a primary cultivation module 2, a secondary cultivation module 3 and a drinking module 4, each module is separated by a heat insulation board Separated, each with a heater at the bottom; the sterilization module is located on the upper part of the main body of the algae juice machine, the primary cultivation module and the secondary cultivation module are located below the sterilization module, and the volume of the primary cultivation module is larger than that of the secondary cultivation module. The drinking module is located below the secondary cultivation module and adjacent to the primary cultivation module:

The algae juice machine is made of transparent polycarbonate (PC) material and aluminum alloy material as a closed cultivation space, which is light in weight, and the closed type can effectively avoid algae pollution during the cultivation process; the material of the primary cultivation module and the secondary cultivation module is Transparent polycarbonate material is used to observe the growth and cultivation of Haematococcus pluvialis in real time. The transparent material greatly increases the light surface area to volume ratio of the algae liquid in the first-level cultivation module, improves the light utilization rate of the reactor, and improves the efficiency of the reactor. The culture density; The material of described heating pot is transparent polycarbonate material.

The algae juice machine is equipped with a main control board 8, as shown in Figure 6, which is composed of a temperature display and a control part. The temperature data obtained by the sensor 15 shows the real-time temperature of the sterilization module, the primary cultivation module, the secondary cultivation module and the drinking module; the control module is composed of two touch buttons of start and stop. The whole system starts to run, touch the stop button and the whole system stops running.

There is a round hole on the top of the sterilization module 1 as the water inlet 5, and a small bucket 21 is placed on the water inlet 5, as shown in Figure 5, the small bucket is connected to the external water supply device through a PVC hose (ordinary bottled mineral water) ;The PVC hose can be installed with a control valve close to the bottled mineral water to prevent sucking back when replacing the water supply bucket.

The side of the sterilization module is equipped with a reagent storage box 6, as shown in Figure 2, the reagent storage box is equipped with nutrient salts needed for the growth of Haematococcus pluvialis; the reagent storage box includes a pressure button 12, a small internal reagent temporary storage Box 14, LED ultraviolet lamp 13, the internal small reagent storage box is ejected by pressing the pressure pop-up button, and the reagent box is retracted after the reagent is added, the ultraviolet lamp is turned on for sterilization, the reagent box is dumped after sterilization, and the reagent is poured into the sterilization module.

There is a water flow control valve 17 at the bottom of the sterilization module, as shown in Figure 3, the side wall of the primary cultivation module 2 has a water level sensor 16, the heater in the sterilization module is a water supply timing heating device, and water enters the sterilization module from the water inlet Then start the heating device and heat it to boiling. After the heating device is turned off, when the temperature monitor cools to 20°C and the water level sensor detects that the water level has dropped, the flow control valve is opened, and the sterilized constant temperature water enters the first-level cultivation module to cultivate Haematococcus pluvialis . There is a float control valve 10 inside the secondary training module, including a lever 19 and a floating ball 20. As shown in Figure 4, a water channel 18 is opened on the side heat shield shared by the primary training module and the secondary training module. , the floating ball and the lever are connected through polypropylene PP filaments, and the lever and the water channel are connected through PC short rods; when the water level of the secondary culture module drops, the floating ball will drop with the water level, and the lever will be pulled to open the water channel, due to the water level difference. A pressure difference is generated to make the green swimming cells in the primary culture module enter into the secondary culture module. A heating pot 11 is placed inside the drinking module, and the heating pot is provided with a reference switch 9. When the drinking switch 9 is opened, the secondary cultivation module 3 Haematococcus pluvialis flows out into the drinking module, and the water level of the secondary cultivation module 3 drops.

The specifications of the algae juice machine are: length 46cm, width 23cm, height 46cm; The lower part of the inlet of the salt replenishment device is 25cm higher than the base, and the thickness of the fuselage partition is uniform, both of which are 3mm.

Example

(1) Water injection: place a small bucket on the water inlet of the fuselage, connect the small bucket to the external water supply device with a PVC hose, turn on the system to open the water supply device to inject water into the equipment, and turn on the heating plate to heat the mineral water in the sterilization module. Sterilize by boiling; when cooled to 20°C, the flow control valve is opened, and the sterilized constant temperature water enters the primary cultivation module for Haematococcus pluvialis cultivation;

(2) Inoculation: the initial inoculation is carried out in the primary culture module, and the initial cell inoculation density is 10×10 ⁴ cells/ml;

(3) Add nutrient salts: Add food-grade nutrient salts for Haematococcus pluvialis into the reagent storage box, press the pressure button, the internal reagent temporary storage box pops up, pour in nutrient salts, and the internal reagent temporary storage box returns to the body. The LED ultraviolet lamp starts to work to sterilize the air and nutrient salts in the kit by radiation; then the internal reagent temporary storage box is poured inward, and the nutrient salts enter the sterilization module and are mixed evenly under the entry of sterilized water. The nutrient salt is professional aseptic configuration, quantitative configuration;

(4) Primary cultivation: the heating plate distributed at the bottom of the primary cultivation module is heated, the temperature reaches 20°C and is maintained at 20°C, and cultured at a constant temperature of 20°C for 5 days. When the liquid level reaches 25cm, it flows into the secondary cultivation module. At the end of the 5th day of culture, samples were taken for cell density measurement and morphological identification. Microscopic examination showed that the cell density of Haematococcus pluvialis was 2.5×10 ⁵ cell/mL, and most of the algal cells were green swimming cells, accounting for 90% of the total number of cells;

(5) Secondary cultivation: heated by a heating plate distributed at the bottom of the secondary cultivation module, the temperature reached 40°C and maintained at 40°C, and cultured at a constant temperature of 4°C for 4 days. After 2 days of culture, algae samples were taken for morphological identification and determination of astaxanthin accumulation. Under the microscope, most of the algal cells had transformed from green swimming cells to red immobile cells, accounting for 96% of the total cells, and the astaxanthin content was 0.634mg/L.

The above examples are only for illustrating the technical concept and technical characteristics of the present invention, and cannot limit the protection scope of the present invention. All equivalent changes or modifications made according to the essence of the present invention shall fall within the protection scope of the present invention.

Claims (1)

1. a kind of family expenses prepare the algae juice machine of haematococcus pluvialis juice, and algae juice owner body is in rectangular shape, including sterilization module (1), one-level culture module (2), two grades of culture modules and drink module (4) at (3), and modules are separated with thermal insulation board respectively；Its Be characterised by, sterilization module, one-level culture module, two grades culture module, drink module respectively have heater, heater heating temperature Degree is set by automated procedures, and each module has temperature sensor (15) to detect that holding modules are transported at a certain temperature OK；A heating kettle (11) is placed in described drinking inside module (4)；

Described heater is heating plate (7), cultivates module positioned at sterilization module, one-level culture module, two grades, drinks module Bottom；

Described algae juice machine is also equipped with master control borad (8), display sterilization module, one-level culture module, two grades of culture modules, drinks mould The real time temperature of block, and start and close operation and closing that two touch control buttons control whole system respectively；

Positioned at the upper part of algae juice owner's body, it is water inlet (5) that top has a circular hole to described sterilization module (1)；Described One-level culture module (2), two grades of cultures module (3) are respectively positioned on the lower section of sterilization module (1), the volume of one-level culture module (2) More than two grades of cultures module (3)；Described module (4) of drinking positioned at two grades of lower sections of culture module (3), and with one-level culture mould Block (2) is adjacent；

A small-sized bucket (21) is placed with described water inlet (5), by polyvinyl chloride hose by small-sized bucket and outside Water supply installation is connected, and outside water supply installation is common barreled mineral water；Polyvinyl chloride hose installs control at barreled mineral water Valve processed；

Described sterilization module side is provided with reagent receiver (6), and reagent receiver needs built with haematococcus pluvialis growing Nutritive salt；Described reagent receiver includes pressure-button (12), the reagent temporary storage box (14) of Internal Small-scale, LED uviol lamps (13), being pressed by pressure type ejector button ejects Internal Small-scale reagent storage box, and kit is withdrawn after adding reagent, ultraviolet Lamp opens sterilizing, and kit is toppled over and for reagent to pour into sterilization module after sterilizing；

The sterilization module bottom has a stream water control valve, and one-level culture sidewalls have level sensor, described Heater in sterilization module is water supply Timed heating device, and water starts heating devices heat after entering sterilization module by water inlet Closed to boiling post heating device, when monitoring temperature is cooled to 20 DEG C and when level sensor detects water level decreasing, flowing water Control valve is opened, and sterilizing thermostatted water carries out culture haematococcus pluvialis into one-level culture module；

Two grades of culture inside modules have a floater controlled valve, including lever and ball float, one-level culture module and two grades of cultures One is provided with the shared side thermal insulation board of module pulls round polycarbonate piece to adjust with SEA LEVEL VARIATION by floater controlled valve control Save the circular hole passage of passage switching, the passage is aquaporin (18), and ball float is connected with lever by polypropylene PP filaments, lever and Aquaporin is connected by makrolon stub；When module water level decreasing is cultivated for two grades, ball float declines with water level decreasing, affects Lever opens aquaporin, produces green swarm cell in pressure official post one-level culture module to enter two grades of cultures due to water-head Module；

Described module (4) of drinking has passage to be opened/closed with two grades of culture module (3) bottoms, and opening and closing is drunk by passage institute band Switch (9) control；

Described algae juice machine is integrally made closed culture space by clear polycarbonate (PC) material and aluminum alloy materials；One-level The material of culture module and two grades of culture modules is transparent polycarbonate material；The material of described heating kettle is transparent poly- carbonic acid Ester material；Described algae juice machine specification is：46cm long, 23cm wide, 46cm high；Thermal insulation board is close to fuselage and causes confined space；Note Water one-level culture module a height of 25cm, two grades of culture a height of 18cm of module, nutritive salt supplementary device entrance bottom is away from base A height of 25cm, fuselage block board thickness is homogeneous, is 3mm.