CN105193649B - With radiation proof and repair the external skin-care composition of skin barrier effect, toner and preparation method thereof - Google Patents
With radiation proof and repair the external skin-care composition of skin barrier effect, toner and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of with radiation proof and the external skin-care composition for repairing skin barrier effect and preparation method thereof, the composition is made up of the raw material of following weight percentage：Chinese medical extract 4wt%~8wt%, butanediol 2wt%~5wt%, glycerine 1wt%~5wt%, EDTA0.01wt%~0.05wt%, preservative 0.01wt%~0.50wt%, water surplus；The bulk drug that the Chinese medical extract is matched by following weight parts is made：Honeysuckle 2~6, the red sage root 1~4, Radix Codonopsis 1~4, the Radix Astragali 1~3, the tuber of dwarf lilyturf 1~2 and cactus 1~2.The present invention is verified by experiments non-stimulated to skin safe, and skin fibroblasts Proliferation Ability has good protective effect to caused by electron radiation, can also effectively remove skin free radical, while has antibacterial and skin barrier concurrently and repair effect.Easy, preparation method simplicity that raw material of the present invention obtain, it is green safe, it is easy to be received by allergic human population, there are good application prospect and market prospects.
Description
Technical field
It is specifically a kind of that there is radiation proof and repair skin barrier work(the present invention relates to a kind of external skin-care composition
The external skin-care composition of effect and the skin care formulation being prepared by the skin care compositions.
Background technology
People is a part of the ecosystem, and people are survived by Environmental change condition, made the life better；Together
When, the change of environment also influences the clothing, food, lodging and transportion -- basic necessities of life of the mankind and the health of the mankind.A variety of shadows be present in the external environment condition that we live
Ring the factor of skin health, ultraviolet, electrical equipment radiation, haze, acid rain, Enviromental pollutants etc. as known to us.In recent years,
As the improvement of people's living standards, people's environmental pollution recognizing dangers improve constantly, in order to protect the skin from the external world
Infringement, research environment contamination hazard and its protection are as one of focus of health care there research.
At present, the skin care grade of infringement type is defendd to be pursued by consumer in market, the skin care item research and development of defence infringement type are not by
Few industry specialists are had an optimistic view of.Major product effect of consumer's concern has haze (preventing air contamination), radiation proof, anti-stimulation etc..
The defence principle of in the market defence infringement type skin care item is main still based on isolation, releive supplemented by.Protection approaches are single (as prevented
Shine product) or unilateral (such as anti-irritant product) or shortage successes achieved in research support, also without the production of comprehensive defence infringement
Product, consumer can only be a in addition in a product of use and then use, add skin care item and use complexity.
In addition, as the gradual popularization of mobile phone and the covering of mobile telephone base station are more and more intensive, people's moment is in hand
In the radiation field of machine.Although the photon energy of mobile phone can also trigger far field less than 10,000,000 times minimum of energy ionizing radiation
The threat of radiation.And skin is one of organ that exposed area is maximum, time of contact is most long, therefore, cell phone microwave radiation is to skin
The influence of skin morphosis also can not be ignored.It was found by the inventors of the present invention that people long-time use mobile phone after, mobile phone radiation
The Proliferation Ability of skin fibroblasts can be caused, pathogenic microorganism increases on skin, so as to destroy skin barrier, causes skin
Aging.Presently commercially available skin-protection product can not effectively suppress mobile phone and other electromagnetic radiation such a damage to caused by skin.
Therefore by removing free radical, the suppression of protection radiation cause fibroblast proliferation, suppressing skin sex pheromone, maintenance skin screen
Hinder effect approach, reach the comprehensive emphasis resisted damage of the environmental pollution to skin and studied for the present invention.
The content of the invention
The primary and foremost purpose of the present invention is to propose a kind of skin care compositions with radiation proof and reparation skin barrier effect.
Another object of the present invention is the toner system for proposing a kind of preparation method of above-mentioned skin care compositions and obtaining
Agent.
The present invention invention thinking be：
The present invention utilizes advanced biotechnology, with reference to the theory of traditional Chinese medical science of Chinese tradition, by honeysuckle, the red sage root, Radix Codonopsis, Huang
Stilbene, the tuber of dwarf lilyturf and cactus are effectively combined in together, form compound, and as active component, are prepared into skin care formulation, its
Radiated with protection and cause fibroblast proliferation to suppress, suppress skin sex pheromone and maintenance skin barrier effect, so as to
Effectively prevention is with improving skin senescence.Each component is described as follows：
Honeysuckle：It is cold in nature, it is sweet, enter lung, the heart, stomach, there is the effect of clearing heat and detoxicating, anti-inflammatory, qi-restoratives treat wind, cure mainly swollen
Completely descend disease, warm disease heating, the disease such as heat toxin large carbuncle and tumour.
The red sage root：It is bitter in taste, it is slightly cold.The thoughts of returning home, Liver Channel.There is a stasis-dispelling and pain-killing, invigorate blood circulation, the effect of relieving restlessness that clears away heart-fire.For the moon
Through uncomfortable, closed dysmenorrhea, a lump in the abdomen causing distension and pain, chest and abdomen shouting pain, hot numbness pain, sore swell and ache curative, dysphoria and insomnia；Hepatosplenomegaly, angina pectoris.
Radix Codonopsis：It is sweet, it is mild-natured.There is tonifying middle-Jiao and Qi, quench the thirst, strengthening spleen and tonifying lung, nourishes blood and promotes the production of body fluid effect.For spleen-lung Qi deficiency, food
Few burnout, false asthma cough, insufficiency of vital energy and blood, sallow complexion, palpitation, injury thirst, Heat Diabetes.
The Radix Astragali：Nature and flavor：It is sweet, tepor.Return lung, spleen, liver and kidney channel.To exterior deficiency spontaneous perspiration, yang-energy weakness, sore diabrosis and lung qi
Asthenic symptoms have certain effect.
The tuber of dwarf lilyturf：Nature and flavor are sweet, slight bitter, are slightly cold.The thoughts of returning home, lung, stomach.Function is with curing mainly：Nourishing Yin and promoting production of body fluid, moistening lung clears away heart-fire.For
Dryness of the lung dry cough, cough for deficiency of Yin consumptive disease, larynx numbness pharyngalgia, injury thirst, Heat Diabetes, vexed insomnia, dry constipation of intestines.
Cactus：Nature and flavor slight bitter, it is cool.The thoughts of returning home, lung, stomach three pass through.With clearing heat and detoxicating, relaxing tendons and activating collaterals, eliminating stasis to subdue swelling, intestines are solved
Poison, cooling blood and relieving pain, ease constipation hemostasis, the effect of benefiting stomach and stopping pain and antibechic.
To realize the purpose of the present invention, the present invention uses following concrete technical scheme：
A kind of external skin-care composition with radiation proof and reparation skin barrier effect, wherein, the composition is under
State the raw material composition of percentage by weight：
The raw material that the Chinese medical extract is matched by following weight parts is made：
Honeysuckle 2~6, the red sage root 1~4, Radix Codonopsis 1~4, the Radix Astragali 1~3, the tuber of dwarf lilyturf 1~2 and cactus 1~2.
The preferable preparation method of the Chinese medical extract is：
(1) by above-mentioned raw materials medicinal powder it is broken after, weighed by the weight, mix；
(2) percent by volume is extracted for 60%~90% ethanol, and bulk drug is 1 with ethanol mass volume ratio g/mL：8~1：
20,70 DEG C~90 DEG C are extracted 1~4 hour；
(3) extract solution that step (2) obtains is cooled to 20 DEG C~30 DEG C, the filtering of 200~400 mesh, it is laggard filters out the dregs of a decoction
Row vacuum filtration, collects filtrate；
(4) filtrate rotary evaporation whole ethanol is obtained into concentrate, 1,3-BDO is added in concentrate, addition is step
Suddenly 60%~90% of filtrate volume percentage obtained by (3)；It is miscible uniform, filtering, clear liquid is taken, is produced.
The percent by volume of ethanol is 70% in the step (2), and bulk drug is 1 with ethanol mass volume ratio g/mL：15；
Extraction time is 3 hours.
Vacuum filtration condition is that two layers of filter paper is spread in Buchner funnel in the step (3), filter paper centre folder 0.3~
0.6cm diatomite is filtered by vacuum.
A kind of preparation method of above-mentioned skin care compositions, wherein, methods described step is as follows：
(1) composition in addition to Chinese medical extract and preservative is added to the water after being heated to 80 DEG C~90 DEG C, stirred,
80 turns~100 revs/min of stir speed (S.S.), after being incubated 0.5 hour~1 hour, cool, 1 DEG C~2 DEG C of rate of temperature fall is per minute；
(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
A kind of external skin-care composition with radiation proof and reparation skin barrier effect, wherein, the composition is under
State the raw material composition of percentage by weight：
The bulk drug that the Chinese medical extract is matched by following weight parts is made：
Honeysuckle 2~6, the red sage root 1~4, Radix Codonopsis 1~4, the Radix Astragali 1~3, the tuber of dwarf lilyturf 1~2 and cactus 1~2.
The preparation method of the Chinese medical extract is the same as above-mentioned.
A kind of preparation method of above-mentioned skin care compositions, wherein, methods described step is as follows：
(1) stir, stir after the component in water in addition to Chinese medical extract and preservative being heated into 80 DEG C~90 DEG C
100 revs/min of speed is mixed, after being incubated half an hour, is cooled, 1 DEG C of rate of temperature fall is per minute；
(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
The skin care formulation is toner, also referred to as watery toner, firming lotion or smoothing toner, utilizes Chinese medicine of the present invention
Other formulations, such as cream, facial mask, spray can also be prepared in extract and skin care field conventional method and auxiliary material.
The preparation method of Chinese medicine composition of the present invention is not limited to this specification record, and field of Chinese medicines customary preparation methods may also function as institute
State effect.The method of the invention is optimal.
Invention formulation recommends method：After daily face cleaning, toner is coated on skin, gently massaged, treats it
Slowly absorb.
The beneficial outcomes of the present invention：
The present invention is verified by experiments, to electron radiation caused by skin fibroblasts propagation suppression non-stimulated to skin safe
Fixture has good protective action, can also effectively remove skin free radical, while has antibacterial and skin barrier concurrently and repair work(
Effect.Easy, preparation method simplicity that raw material of the present invention obtain, it is green safe, it is easy to be received by allergic human population, well should has
With prospect and market prospects.
Brief description of the drawings
Fig. 1 Chinese medicine compositions A, B, C DPPH clearance rate figures.
Embodiment
Medicinal material used in the present embodiment can be commercially available from China Medicament Group Corp., meet People's Republic of China's medicine
2005 editions standards of allusion quotation.Other raw materials can be obtained by commercially available purchase, and the present invention is shown in Table 1 using the source of raw material, institute of the present invention
Instrument title and producer are shown in Table 2.
Table 1
Table 2
| Title | Model | Producer |
| Thermostat water bath | HH·S1-M | Beijing Chang'an scientific instrument factory |
| High-speed multifunctional pulverizer | JP-400B-8 | Yongkang City nine grades of rank in the feudal regimes Trade Co., Ltd. |
| Intelligent temperature control heating stirrer | SZCL | Yu Hua Instrument Ltd. of Gongyi City |
| Rotary evaporator | RE-2000 | Shanghai Yarong Biochemical Instrument Plant |
| Circulating water type vavuum pump | SHB-Ⅲ | Zhengzhou Greatwall Scientific Industrial ＆ Trading Co., Ltd. |
The preparation of 1 Chinese medicine composition of the present invention of embodiment
(1) after being crushed according to following raw material medicaments, weigh, mix by the weight；
Honeysuckle 40g, red sage root 20g, Radix Codonopsis 20g, Radix Astragali 20g, tuber of dwarf lilyturf 10g and cactus 10g.
(2) percent by volume is extracted for 70% ethanol, and bulk drug is 1 with ethanol mass volume ratio g/mL：15, it is positioned over electricity
In hot thermostat water bath, 80 DEG C are extracted 3 hours；
(3) extract solution that step (2) obtains is taken out from thermostat water bath, standing is cooled to normal temperature (20 DEG C), 200 mesh
Coarse filtration, the dregs of a decoction are filtered out, then be filtered by vacuum, filter condition：Two layers of filter paper is spread in Buchner funnel, is pressed from both sides among filter paper
0.5cm diatomite is filtered by vacuum, and collects filtrate；
(4) rotary evaporation whole ethanol obtains concentrate under the conditions of 0.1,43 DEG C of vacuum；1,3- fourths are added in concentrate
Glycol, addition are 70% of filtrate volume percentage obtained by step (3), miscible uniform.Vacuum filtration, it is rapid to filter conditional synchronization
(3) clear liquid, is taken, is produced.
The preparation of 2 Chinese medicine composition of the present invention of embodiment
(1) after being crushed according to following raw material medicaments, weigh, mix by the weight；
Honeysuckle 60g, red sage root 10g, Radix Codonopsis 10g, Radix Astragali 10g, tuber of dwarf lilyturf 20g and cactus 10g.
(2) percent by volume is extracted for 90% ethanol, and bulk drug is 1 with ethanol mass volume ratio g/mL：8, it is positioned over electricity
In hot thermostat water bath, 70 DEG C are extracted 4 hours；
(3) extract solution that step (2) obtains is taken out from thermostat water bath, standing is cooled to normal temperature, the thick mistake of 200 mesh
Filter, filters out the dregs of a decoction, obtains filtrate；The filtrate is filtered by vacuum again, filters condition：Two layers of filter paper is spread in Buchner funnel, is filtered
Folder 0.3cm diatomite vacuum filtration, collects filtrate among paper；
(4) rotary evaporation whole ethanol obtains concentrate under the conditions of 0.1,43 DEG C of vacuum；1,3- fourths are added in concentrate
Glycol, addition are 90% of filtrate volume percentage obtained by step (3), miscible uniform.Vacuum filtration, it is rapid to filter conditional synchronization
(3) clear liquid, is taken, is produced.
The preparation of 3 Chinese medicine composition of the present invention of embodiment
(1) after being crushed according to following raw material medicaments, weigh, mix by the weight；
Honeysuckle 20g, red sage root 40g, Radix Codonopsis 40g, Radix Astragali 30g, tuber of dwarf lilyturf 10g and cactus 20g.
(2) percent by volume is extracted for 60% ethanol, and bulk drug is 1 with ethanol mass volume ratio g/mL：20, it is positioned over electricity
In hot thermostat water bath, 90 DEG C are extracted 1 hour；
(3) extract solution that step (2) obtains is taken out from thermostat water bath, standing is cooled to normal temperature, the thick mistake of 100 mesh
Filter, filters out the dregs of a decoction, obtains filtrate；The filtrate is filtered by vacuum again, filters condition：Two layers of filter paper is spread in Buchner funnel, is filtered
Folder 0.6cm diatomite vacuum filtration, collects filtrate among paper；
(4) rotary evaporation whole ethanol obtains concentrate under the conditions of 0.1,43 DEG C of vacuum；1,3- fourths are added in concentrate
Glycol, addition are 60% of filtrate volume percentage obtained by step (3), miscible uniform.Vacuum filtration, it is rapid to filter conditional synchronization
(3) clear liquid, is taken, is produced.
The preparation of 4 toner of the present invention of embodiment
Raw material and proportioning (mass percent)
Preparation method：
(1) composition in addition to Chinese medical extract and preservative is added to the water after being heated to 80 DEG C, stirred, stirring speed
80 revs/min of rate, after being incubated 1 hour, cool, 2 DEG C of rate of temperature fall is per minute；
(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
The preparation of 5 toner of the present invention of embodiment
Raw material and proportioning (mass percent)
Preparation method：
(1) composition in addition to Chinese medical extract and preservative is added to the water after being heated to 85 DEG C, stirred, stirring speed
90 revs/min of rate, after being incubated 40 minutes, cool, 1 DEG C of rate of temperature fall is per minute；
(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
The preparation of 6 toner of the present invention of embodiment
Raw material and proportioning (mass percent)
Preparation method：
(1) composition in addition to Chinese medical extract and preservative is added to the water after being heated to 80 DEG C, stirred, stirring speed
100 revs/min of rate, after being incubated half an hour, cool, 1 DEG C of rate of temperature fall is per minute；
(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
The preparation of 7 toner of the present invention of embodiment
Raw material and proportioning (mass percent)
Preparation method：
(1) composition in addition to Chinese medical extract and preservative is added to the water after being heated to 90 DEG C, stirred, stirring speed
100 revs/min of rate, after being incubated half an hour, cool, 1 DEG C of rate of temperature fall is per minute；
(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
Efficacy experiments of the present invention
First, embodiment 1, embodiment 2, the evaluation of embodiment 3 gained Chinese medical extract A, B, C anti-radiation effect
(1) research purpose
By observing Chinese medical extract A, B, C (being prepared respectively by embodiment 1, embodiment 2 and embodiment 3) to mobile phone
The influence that Mouse Skin Fibroblasts strain 3T6 grows after radiation, evaluation Chinese medicine composition A, B, C suppress cell to mobile phone radiation
The protective effect of growth.
(2) experiment material
By test product：Embodiment 1, embodiment 2, embodiment 3 gained Chinese medical extract A, B, C
Cell line：Mouse Skin Fibroblasts strain 3T6, purchased from Chinese Academy of Sciences's cell bank, make after this laboratory passage
With.
Reagent：FBS (lot numbers：1428479), 0.25%Trypsin-EDTA (lot numbers：1213981) GIBCO companies are purchased from.
Modified form RPMI-1640 type culture medium (lot numbers：NXL0745 HYCLONE companies) are purchased from.DMSO (lot numbers：20121102) it is purchased from
Chemical Reagent Co., Ltd., Sinopharm Group.Cell proliferation-toxicity detection-CCK-8 kits are Japanese colleague DOJINDO chemistry
Research institute's product.
Laboratory apparatus：X71 types inverted microscope is Olympus Products.HH CP-01W type carbon dioxide cell cultures
Case is Medical Equipment Plant of Shanghai Boxun Industrial Co., Ltd. product.The single one side clean work station of SW-CJ-IFD types is that Suzhou is net
Change equipment Co., Ltd product.RT-6000 type ELIASAs, Shenzhen thunder Du's Products.The type of Nokia's mobile phone 1050, frequency range are
GSM900MHz。
(3) experimental method
Experiment is divided into blank control group, radiation control group, medicine various concentrations group, every group of 8 multiple holes.It is thin to collect logarithmic phase
Born of the same parents, count, by 1 × 104/ mL concentration is inoculated into 96 orifice plates, per the μ L of hole 200.37 DEG C are put, 5%CO224 are cultivated in cell culture incubator
Hour, make cell attachment.Medication group adds the different μ L of Chinese medical extract 100, and blank control group and radiation control group add 100 μ L
Serum free medium, continue to cultivate.After 24 hours, cell plates are placed in CO2Incubator exoadaptation 1 hour, except blank control group
Outside cell plates, other cell plates are placed on above mobile phone, mobile phone keeps talking state, and blank control group cell plates are in another room
Between be placed in above mobile phone, mobile phone remains off.Using CCK methods detection cytoactive after 6 hours, and calculate proliferation inhibition rate
And Proliferation Ability protective rate.
(4) experimental result
As a result show, after mobile phone radiation 6 hours, skin fibroblasts propagation is suppressed so that absorbance drops
Low, radiation cellular control unit OD values relatively have significant difference (P compared with blank control group<0.01), proliferation inhibition rate reaches
16.89%；Chinese medical extract A, B, C dosage are in 1000 μ gmL-1Shi Jun has obvious protect to cell inhibitory effect after radiation
Shield acts on, and radiation can be effectively reduced to fibroblastic inhibitory action, so as to which absorbance rises, with radiating control group ratio
Relatively there is significant difference (P<0.05).(being shown in Table 3) Chinese medical extract A's is reachable to fibroblast proliferation suppression protective rate
11.86%.
Influences of table 3 Chinese medical extract A, B, the C to cell propagation/toxicity after radiation
| Group | Concentration μ gmL-1 | Absorbance | Proliferation inhibition rate (%) | Proliferation Ability protective rate (%) |
| Chinese medical extract A | 1000 | 1.18±0.09* | -- | 11.86 |
| Chinese medical extract B | 1000 | 1.15±0.07* | -- | 9.57 |
| Chinese medical extract C | 1000 | 1.13±0.05* | -- | 7.96 |
| Radiate control group | -- | 1.04±0.09## | 16.89 | -- |
| Blank control group | -- | 1.26±0.05 | -- |
2nd, embodiment 1, embodiment 2, gained Chinese medical extract A, B, the C of embodiment 3 to radiate indirectly-acting protection effect
Radiating the effect to bioactive substance has two approach：First, directly acting on large biological molecule such as DNA, pass through
The fracture for bringing it about chemical bond is ionized and excited, causes the change of molecular structure and the forfeiture of bioactivity, this effect claims
Directly to act on；Second, ray interacts with hydrone, the activation products such as free radical are produced, the latter is remake for biology point
Son, causes its damage, and such effect is referred to as indirectly-acting.In vivo, due to a large amount of presence of hydrone, indirectly-acting tool
There is prior meaning.Therefore, for radiate indirectly-acting, can select remove free radical effect come evaluate Chinese medical extract A,
B, the protection effects of C to radiation indirectly-acting.
By the gained Chinese medical extract of embodiment 1~3 and deionized water with 3：97 mass ratio mixes, and obtains Chinese medical extract
The aqueous solution；Test group is used as using the aqueous solution of the Chinese medical extract；Wherein, the gained Chinese medical extract A counter samples of embodiment 1
1；The gained Chinese medical extract B counter samples 2 of embodiment 2；The gained Chinese medical extract C counter samples 3 of embodiment 3；By in test group
Chinese medical extract replace with vitamin C derivatives, obtained aqueous solution is as positive control.
Measurement result such as Fig. 1.Test result indicates that quality hundred of the Chinese medical extract in its aqueous solution obtained by each embodiment
When point content is 3.0%, there is obvious DPPH Scavenging activities, has that to reduce hydroxy radical, alkane free radical or oxidation free
The valid density of base and the effect for interrupting lipid peroxidation chain reaction, so as to the work with good delaying skin radiative aging
With.
3rd, the bacteria resistance function evaluation of embodiment 1, embodiment 2, embodiment 3 gained Chinese medical extract A, B, C
The air pollutions such as haze, the content of microorganisms in environment is made to increase, wherein common Escherichia coli, golden yellow grape
Coccus, propionibacterium acnes contact skin easily cause skin acne, it is sensitive phenomena such as.
Embodiment 1~3 gained Chinese medical extract A, B, C are subjected to Escherichia coli, staphylococcus aureus, acne propionic acid bar
Bacterium suppresses efficacy assessments.
Odontothrips loti：Corresponding culture medium, 121 DEG C of high pressure steam sterilization 20min are configured according to different strains testeds.Make solid
Body culture medium flat plate, each culture dish about pours into 20mL culture mediums, and after culture medium solidifying, 100 μ L bacterium solutions have been taken (with liquid-transfering gun
By the bacteria suspension of Maxwell turbidimetry for Determination concentration) instill in culture dish, using spread plate, bacteria suspension is spread evenly across
On solid medium.Media surface directly vertically put Oxford cup (internal diameter 6mm, external diameter 8mm, high 10mm it is circular small
Pipe), gently pressurize, it is contacted tight with culture medium.Each culture dish places three Oxford cups.Added in each Oxford cup
200 μ L testing samples, the culture dish of bacterium is gently placed in bacteriological incubator or so 37 DEG C of cultures, 16 hours.
Wherein, the gained Chinese medical extract A counter samples 1 of embodiment 1；The gained Chinese medical extract B counter samples 2 of embodiment 2；
The gained Chinese medical extract C counter samples 3 of embodiment 3；Positive control is 0.2%MTI (methylisothiazolinones/iodine propinyl fourth
Methyl carbamic ester).MTI is the preservative commonly used in cosmetics, it is proposed that dosage is 0.05%~0.2%.Have to bacterium, fungi
Excellent antibacterial activity.Measurement result such as table 4.
Table 4 Chinese medical extract A, B, C bacteria resistance function is evaluated
Note：Antibacterial circle diameter >=20mm has been judged to bacteriostasis for extremely sensitive " +++ ", and the bigger fungistatic effect of inhibition zone is more
It is good；15mm≤bacteriostatic diameter<20mm is Gao Min " ++ "；10mm≤bacteriostatic diameter<15mm quick "+" in being；Bacteriostatic diameter<10mm is
It is invalid, it is judged to no bacteriostasis.With reference to susceptibility test methods [J] animal and veterinary scientific and technological informations of Chen Yu duckweed antibacterials,
2007, (5)：88-89.
From experimental result as can be seen that sample 1, sample 2, sample 3 are respectively provided with good fungistatic effect, can effectively reduce
Adverse effect of the microorganism to skin in environment.
4th, embodiment 6, the evaluation of the anti-radiation effect of the gained preparation of embodiment 7
The gained preparation of embodiment 6 is corresponded into toner 2；The gained preparation of embodiment 7 corresponds to toner 3.
Negative control is separately used as using table 5.Negative control toner preparation method used is as follows：
It is formulated according to the gained of table 5, first inhibiting factor, oat polypeptide, butanediol, glycerine, EDTA, allantoin will be stimulated to add
After 80 DEG C~90 DEG C are heated in deionized water, stir cooling；Less than 40 DEG C are cooled to, the preservative is added and stirs.
The negative control toner formula of table 5
| Material name | Dosage (wt%) |
| Stimulate inhibiting factor | 2.0 |
| Oat polypeptide | 8.0 |
| Butanediol | 2.0 |
| Glycerine | 5.0 |
| EDTA | 0.05 |
| Allantoin | 0.1 |
| Deionized water | Surplus |
| Preservative | 0.5 |
Kunming kind hairless mouse, 60, male and female half and half, 18~22g of body weight, tieing up tonneau China experimental animal technology by Beijing has
Limit company provides.
Experimental method：60 mouse are randomly divided into 6 groups, i.e.,：1. Normal group, control group is 2. radiated, 3. 1 group of sample,
4. 2 groups of sample, 5. 3 groups of sample, 6. negative control group.Every group 10, by sex and group sub-cage rearing, under identical environment,
Temperature is controlled to maintain 20~25 DEG C, 12h illumination, 12h is dark, and conventional feeding drinking-water, no undesirable element influences, and raises one week and adapts to
Experiment is proceeded by after laboratory environment.
Mobile phone is placed in the cage exclusively for this experimental design (diameter 45cm, high 11cm).Using Nokia
1050 type mobile phones (104 × 44 × 17) carry out radiation test as radiation source.
60 mouse are randomly divided into 6 groups (every group 10), one group is not smeared sample, and mobile phone remains turned-off shape during experiment
State, as Normal group.One group is not smeared sample, and mobile phone keeps opening state during experiment, and when when daily 10,14,
When 16, in an ON state, 15min is connected every time, as radiation control group.Other four groups are used as experimental group, during experiment, hand
Machine keeps opening state, and when when daily 10,14,16 when, in an ON state, connect 15min every time.Sample is smeared respectively
1, sample 2, sample 3, negative control cosmetic water sample.Sample is uniformly embrocated respectively in mouse back, is 0.5mL every time,
Once a day, mouse is placed in the cage of radiation source after smearing uniformly, each 45min, once a day, continues 96 days.
96th day, mouse back apparatus is lost hair or feathers, area is 2cm × 3cm.After disconnected neck puts to death whole mouse, remove immediately
Full thickness skin (being free of subcutaneous fat) at the depilation of back, it is to be measured to be frozen in preservation in -70 DEG C of refrigerators.Using histopathologic slide's system
Chip technology, HE dyeing, is changed with 40 times of om observation dermis, and with biochemical analysis method measure SOD activity, MDA contents.
SOD can remove super oxygen cation (i.e. oxygen radical), protect cells from damaging.SOD vigor height can between it is reversed
The ability of film projector body scavenging activated oxygen.Unrighted acid in free radical attack film fat, brings it about oxidation, lipid peroxidation
The catabolite MDA of matter will be raised, and by determining internal MDA contents, can reflect damage of the interior free yl to body indirectly
Degree.
All data are handled by SPSS19.0 softwares, and each group experiment parameter is represented with x ± s.
SOD, MDA content (x ± s) (n=10) in each group hairless mouse skin tissue of table 6
SOD, MDA content are shown in Table 6 in mouse skin.As a result show：Embodiment 6 (counter sample 2), (the corresponding sample of embodiment 7
Product 3) it is respectively provided with radioresistance effect.The toner that inventor is prepared using embodiment 4,5 repeats above-mentioned experiment, is as a result same as above.
5th, the maintenance barrier evaluation of present invention gained preparation
1. cuticula moisture loss is tested
Test sample is radiation proof toner, prepares and obtains according to embodiment methods described.
Blank control is separately used as using the toner of table 5.The preparation method of blank control toner used and the radiation proof of preparation
Negative control preparation method in efficacy assessments is identical.
Test before through the trained health volunteer 30 of professional (each 15 of men and women), the age at 20~30 years old, institute
Have subject without skin or systemic disease history, recipient site is without exception, and tested period do not smear it is any unrelated with experiment
Medicine or cosmetics.
Test site room temperature (25 ± 1) DEG C, relative humidity (40 ± 5) %, subject should keep body as stabilization before test
State.After wiping subject's both arms using 35 DEG C or so of clear water, start to test after the 30min that sits quietly under test environment.
At subject's inboard arm is away from palm base portion 5cm, tested region (4cm × 4cm) is marked, same arm can be simultaneously
Mark multiple regions (interval 1cm), the equal random distribution of test sample.The blank value of each test zone is first measured, then test specimens
By (2.0 ± 0.1) mg/cm2Dosage carry out single application, Multi-functional skin moisture tester and moisture of skin are used after coating
Lost instrument measures the moisture loss value in the tested region of 1h, 2h and 4h and blank control region respectively.
Subject is smeared 5 data measured in same sample region and averaged by test, using SPSS statistical softwares
Carry out t check analyses.
Experimental result
Moisture of skin, which scatters and disappears, to be changed
Moisture of skin windage (Transepidermal water loss, TEWL) is reflected in test period, test block
Domain moisture loss changes with time rule, and it can be with the skin barrier maintenance function of characterization test sample, its smaller, moisture of value
Scatter and disappear fewer, skin repair ability is stronger；Conversely, skin repair ability is weaker.
Percentage of water loss (%)=(sample sets water loss amount-starting moisture windage)/starting moisture windage × 100
As a result show：In 4h, embodiment 4,5,6,7 has skin repair ability effect.
Claims (10)
- A kind of 1. external skin-care composition with radiation proof and reparation skin barrier effect, it is characterised in that the external application shield Skin composition is made up of the raw material of following weight percentage：The bulk drug that the Chinese medical extract is matched by following weight parts is made：Honeysuckle 2~6, the red sage root 1~4, Radix Codonopsis 1~4, the Radix Astragali 1~3, the tuber of dwarf lilyturf 1~2 and cactus 1~2.
- 2. external skin-care composition as claimed in claim 1, it is characterised in that the preparation method of the Chinese medical extract is：(1) after bulk drug described in claim 1 is crushed, weigh, mix by the weight；(2) percent by volume is extracted for 60%~90% ethanol, and bulk drug is 1 with ethanol mass volume ratio g/mL：8~1：20, 70 DEG C~90 DEG C are extracted 1~4 hour；(3) extract solution that step (2) obtains is cooled to 20 DEG C~30 DEG C, the filtering of 200~400 mesh, carried out very after filtering out the dregs of a decoction Sky filters, and collects filtrate；(4) filtrate rotary evaporation whole ethanol is obtained into concentrate, 1,3-BDO is added in concentrate, addition is step (3) the 60%~90% of gained filtrate volume percentage；It is miscible uniform, filtering, clear liquid is taken, is produced.
- 3. external skin-care composition as claimed in claim 2, it is characterised in that the percent by volume of ethanol in the step (2) For 70%, bulk drug is 1 with ethanol mass volume ratio g/mL：15；Extraction time is 3 hours.
- 4. external skin-care composition as claimed in claim 2, it is characterised in that in the step (3) vacuum filtration condition be Two layers of filter paper is spread in Buchner funnel, folder 0.3~0.6cm diatomite is filtered by vacuum among filter paper.
- 5. the preparation method of external skin-care composition described in any one in a kind of Claims 1-4, it is characterised in that described Preparation method step is as follows：(1) by the component in water in addition to the Chinese medical extract and preservative, after being heated to 80 DEG C~90 DEG C, stir, 80 turns~100 revs/min of stir speed (S.S.), after being incubated 0.5~1 hour, cool, 1 DEG C~2 DEG C of rate of temperature fall is per minute；(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
- A kind of 6. external skin-care composition with radiation proof and reparation skin barrier effect, it is characterised in that the external application shield Skin composition is made up of the raw material of following weight percentage：The bulk drug that the Chinese medical extract is matched by following weight parts is made：Honeysuckle 2~6, the red sage root 1~4, Radix Codonopsis 1~4, the Radix Astragali 1~3, the tuber of dwarf lilyturf 1~2 and cactus 1~2.
- 7. external skin-care composition as claimed in claim 6, it is characterised in that the preparation method of the Chinese medical extract is：(1) according to above-mentioned raw materials medicinal powder it is broken after weigh by the weight, mix；(2) percent by volume is extracted for 60%~90% ethanol, and bulk drug is 1 with ethanol mass volume ratio g/mL：8~1：20, 70 DEG C~90 DEG C are extracted 1~4 hour；(3) extract solution that step (2) obtains is cooled to 20 DEG C~30 DEG C, 200~400 mesh coarse filtrations, carried out after filtering out the dregs of a decoction Vacuum filtration, collect filtrate；(4) filtrate rotary evaporation whole ethanol is obtained into concentrate, 1,3-BDO is added in concentrate, addition is step (3) the 60%~90% of gained filtrate volume percentage；It is miscible uniform, filtering, clear liquid is taken, is produced.
- 8. external skin-care composition as claimed in claim 7, it is characterised in that the percent by volume of ethanol in the step (2) For 70%, bulk drug is 1 with ethanol mass volume ratio g/mL：15；Extraction time is 3 hours.
- 9. external skin-care composition as claimed in claim 7, it is characterised in that in the step (3) vacuum filtration condition be Two layers of filter paper is spread in Buchner funnel, folder 0.3~0.6cm diatomite is filtered by vacuum among filter paper.
- 10. the preparation method of external skin-care composition described in any one in a kind of claim 6 to 9, it is characterised in that described Preparation method step is as follows：(1) stirred after the component in water in addition to Chinese medical extract and preservative being heated into 80 DEG C~90 DEG C, stirring speed 80 turns~100 revs/min of rate, after being incubated 0.5~1 hour, cool, 1 DEG C~2 DEG C of rate of temperature fall is per minute；(2) less than 40 DEG C are cooled to, the preservative and Chinese medical extract is added and stirs, produce.
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