CN105474959A - Pinus massoniana tissue culture seedling twig short shoot cutting seedling method - Google Patents

Abstract

The invention discloses a Masson pine tissue culture seedling tender branch short spike cutting seedling method, including container selection, matrix preparation, cutting collection, cutting treatment, cutting and nursery management process, select a suitable seedling container, prepare the matrix according to a certain ratio and disinfection method, collect the juvenile tender branch as cutting and carry out elution disinfection treatment, directly carry out cutting after being treated with a root promoter, strengthen the control of light, temperature, water and fertilizer conditions during the period, and the cutting takes root in about one month, the rooting rate reaches more than 98%, and the survival rate reaches more than 97%. The present invention uses Masson pine tissue culture seedlings as the mother plant for cutting, on the one hand, overcomes the problem of long tissue culture succession cycle and high seedling cost, and at the same time, the reproduction multiple is high, the number of qualified juvenile spike strips available for cutting is large, and cutting rooting is very easy, the effect is remarkable, and there is good economic benefit, social benefit and ecological benefit.

Description

Method for Seedling Cultivation of Pine Masson Tissue Cultured Seedlings by Cutting Short Branches and Short Spikes

technical field

The invention belongs to the technical field of plant propagation, and relates to tissue culture and seedling raising technology, in particular to a method for raising seedlings of pine tissue cultured seedlings with short branches and short spikes.

Background technique

Masson pine ( Pinusmassoniana Lamb.) is the main tree species for afforestation in southern China. Due to the dual-purpose characteristics of resinous wood, it has high comprehensive utilization value and broad prospects for promotion. In recent years, with the intensification of contradictions between people's growing living needs and the shortage of timber resources and the gradual depletion of non-renewable resources such as petroleum, the sustainable development of ecology, economy and society has attracted more and more attention from the society. Based on the problems of ecological safety, resource security and social security in our country, it is necessary to vigorously develop the masson pine plantation as the main industrial timber tree species and biomass energy tree species in our country.

Guangxi is in the forefront of the country in the collection, breeding and preservation of Pinus massoniana germplasm resources. Since the "Sixth Five-Year Plan" national scientific and technological breakthroughs, a large number of excellent clones of Pinus massoniana have been selected and bred. The development of modern forestry pays attention to fast growth, high yield, high quality and high efficiency. To achieve the expected goal, the most effective way is to realize the afforestation of high-quality clone seedlings. The use of tissue culture and cutting clonal rapid propagation technology is an effective way to realize the industrialization of masson pine clones.

At present, the mother plants of Pinus massoniana are mainly seedlings, cutting seedlings, and grafted seedlings, and the reports of cutting with tissue-cultured seedlings as mother plants are still rare. Masson pine cutting rooting has an obvious age effect, that is, the younger the cuttings are, the easier the cutting rooting is. Studies have found that when cuttings and grafted seedlings are used as the mother plant for picking ears, the obtained spikes are seriously aging, difficult to re-young, have a strong memory of the age of the mother tree, and the rooting rate is extremely low; The mother plant has a low reproductive coefficient of spikes.

Contents of the invention

The invention overcomes the technical problems of low cutting propagation coefficient, unsatisfactory rooting rate and unstable rooting effect in the existing technology of masson pine cutting seedling raising, and provides a masson pine group with high yield of qualified cuttings, high survival rate of cutting rooting and remarkable effect. The method for raising seedlings by cutting short shoots and short spikes for cultivating seedlings.

In order to realize above object of the invention, technical scheme of the present invention is as follows:

A method for raising seedlings of masson pine tissue cultured seedlings with short branches and short ears, including container selection, substrate preparation, cuttings collection, cuttings treatment, cuttings and nursery management procedures, selecting a suitable seedling container, and preparing the substrate according to a certain ratio and disinfection method Finally, the juvenile twigs are collected as cuttings and subjected to elution and disinfection treatment, and the cuttings are directly carried out after being treated with a root-stimulating agent. as follows:

(1) Container selection: Select a 70-hole seedling tray with a hole size of 6cm×8cm and serrated holes on the side of the hole as a container for cutting seedlings;

(2) Substrate preparation: put the seedling substrate into a non-woven seedling bag with a size of 8cm×9cm, then place it in a seedling tray, then pour the packed seedling substrate into water, sterilize it for later use, and put it in the seedling bag during cutting The water content of the seedling substrate is controlled at 65-75%;

(3) Collection of cuttings: Select young twigs of Pinus massoniana that are 3-4cm long, more than 4mm in diameter, initially lignified, and have clusters of needles at the top, which are soft and vigorous, as cuttings;

(4) Cutting treatment: quickly immerse the collected cuttings in the treatment solution to wash off and disinfect the cuttings for 5-10 minutes, then wash them with clean water for use;

(5) Cutting: Soak the treated cuttings in the root-stimulating agent for 4-12 hours, then use talcum powder to make the root-stimulating agent into a slurry, dip the cuttings in the slurry and insert them directly into the seedling substrate prepared in step (2) In the middle, by spraying and moisturizing, the relative humidity of the seedling raising environment is controlled to 90-95%, the temperature is 20-30°C, and the light is 1500-2000lx. After about 1 month, new shoots are slightly drawn from the top of the cuttings, and the root system is formed to obtain rooted cuttings;

(6) Nursery management: move the rooted cuttings to the field nursery, control the conditions of light, temperature, water and fertilizer within the first 2 months, and manage water, fertilizer and pests and diseases according to conventional seedling raising methods after 2 months.

The raw materials of the seedling-raising substrate described in the above step (2) include fermented pine bark, coconut bran, yellow heart soil and pumice, and the pine bark, coconut bran, yellow heart soil, and pumice are mixed uniformly by volume ratio = 2:2:4:2 to obtain Seedling substrate.

The disinfection described in the above step (2) is a mixed solution with a volume concentration of 0.1-0.3% carbendazim + a volume concentration of 0.5% potassium permanganate.

The treatment liquid described in the above step (4) is a mixed solution of volume concentration 1.5% absolute ethanol + volume concentration 0.1% malonic acid + volume concentration 0.1-0.2% mancozeb + volume concentration 0.02% streptomycin.

The root promoting agent described in the above step (5) contains: NAA50-100mg/L, IBA0-50mg/L, ABT ₁ 0-50mg/L, 6-BA5-10mg/L, VB ₁ 50-100mg/L, A mixed preparation of VB ₆ 20-50mg/L, niacin 20-50mg/L, biotin 10-30mg/L, KNO ₃ 850mg/L, KH ₂ PO ₄ 240mg/L.

In the nursery management described in the above step (6), within the first month, pay attention to shading, control the light at 1500-2000lx, the temperature at 20-30°C, and the relative humidity at 75-85%. Use volume every 7-10 days Spray foliar fertilizer with high-phosphorus compound fertilizer with a concentration of 1‰6N-35P-10K; within the second month, pay attention to ventilation and moisture retention, with a relative humidity of 60-70%, and use a volume concentration of 1-3‰30N every 10-14 days -13P-8K high-nitrogen compound fertilizer was sprayed as foliar fertilizer.

Compared with the prior art, the advantages and positive effects of the present invention are as follows:

1. In the present invention, the tissue-cultured seedlings of Pinus massoniana are used as the mother plant for picking ears. The sprouts are obviously juvenile, vigorous, and the reproduction coefficient is 10-30. The rooting rate of cuttings and cuttings reaches more than 98% in one month, and the survival rate reaches more than 97%. , greatly improving the yield and cutting effect of qualified cuttings, with obvious progress and remarkable benefits.

2. According to the growth characteristics of the masson pine tissue culture seedlings, the present invention determines the technical measures such as the container specification, substrate ratio, cutting ear type, cutting ear processing method, and root-stimulating agent formula adapted to the masson pine cutting seedling cultivation, and manages the seedlings after the cutting. It shows that the whole technology is reliable, easy to operate, good seedling raising effect, great potential for transformation of results, and broad prospects for popularization and application.

3. The present invention uses the tissue-cultured seedlings of Pine massoniana as the mother plant for picking ears to carry out cuttings. On the one hand, it overcomes the problems of long tissue culture subculture cycle and high seedling raising costs, and at the same time has a high multiplication factor, which can be used for qualified juvenile panicles for cuttings. The number of cuttings is large, it is easy to take root from cuttings, the effect is remarkable, and it has good economic, social and ecological benefits.

Reference sign

Fig. 1 is the mother plant of masson pine tissue culture seedling picking fringe in the present invention.

Fig. 2 is the rooted cutting seedling of Pinus massoniana in the present invention.

Fig. 3 is that the cuttage of Pinus massoniana obtained by the present invention becomes a living seedling.

detailed description

The present invention will be further described below in conjunction with specific examples.

Example 1:

The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A 70-hole seedling tray with a hole size of 6 cm x 8 cm and serrated holes on the side of the hole is selected as a container for cutting seedlings. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising matrix with water, and use a mixture of volume concentration 0.1% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising matrix for later use. When cutting, the seedling-raising matrix in the seedling-raising bag The water content is controlled at 65%.

Select the newly pumped 3cm long, more than 4mm in diameter, initial lignification, soft and vigorous juvenile Pinus massoniana twigs with clusters of needles at the top as cuttings; the collected cuttings are quickly immersed in a volume concentration of 1.5% The cuttings are eluted and disinfected in the treatment solution obtained by mixing water ethanol + 0.1% malonic acid + 0.1% mancozeb + 0.02% streptomycin. Soak in the treatment solution for 10 minutes, then wash with clean water for later use.

Put the treated cuttings into a mixture containing NAA50mg/L, 6-BA5mg/L, VB ₁ 50mg/L, VB ₆ 20mg/L, niacin 20mg/L, biotin 10mg/L, KNO ₃ 850mg/L, KH ₂ Soak in the root-promoting agent formed by the mixed preparation of PO ₄ 240mg/L for 12 hours, then use talcum powder to make the root-promoting agent into a slurry, dip the cuttings in the slurry and insert them directly into the prepared seedling substrate, and spray to moisturize to control seedling growth The relative humidity of the environment is 90-95%, the temperature is 20-30°C, and the light is 1500-2000lx. After about one month, new shoots will emerge from the top of the cuttings, and the root system will form. Rooted cuttings are obtained, and the rooting rate is 98.3%.

Move the rooted cuttings to the field nursery. In the first month, pay attention to shading, control the light at 1500-2000lx, the temperature at 20-30°C, and the relative humidity at 75-85%. Use a volume concentration of 1‰6N- Spray foliage fertilizer with 35P-10K high-phosphorus compound fertilizer; within the second month, pay attention to ventilation and moisture, relative humidity 60-70%, and use high-nitrogen compound fertilizer with a volume concentration of 3‰30N-13P-8K every 10 days Spray foliage fertilizer; after 2 months, carry out water, fertilizer and pest management according to the conventional seedling raising method. Statistics show that the survival rate of masson pine seedlings is 97.8%.

Example 2:

The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A seedling raising tray with 70 holes with a hole size of 6 cm x 8 cm and serrated holes on the side of the holes is selected as a container for cutting seedlings. Mix evenly with fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising substrate with water, and use a mixture of volume concentration 0.3% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising substrate for later use. When cutting, the seedling-raising substrate in the seedling-raising bag The water content is controlled at 75%.

Select the newly pumped 4cm long, more than 4mm in diameter, initial lignification, soft and vigorous juvenile Pinus massoniana twigs with clusters of needles at the top as cuttings; the collected cuttings are quickly immersed in a volume concentration of 1.5% The cuttings are eluted and disinfected in the treatment solution obtained by mixing water ethanol + 0.1% malonic acid + 0.2% mancozeb + 0.02% streptomycin. Soak in the treatment solution for 5 minutes, then rinse with clean water for later use.

Put the treated cuttings into a mixture containing NAA100mg/L, 6-BA10mg/L, VB ₁ 100mg/L, VB ₆ 50mg/L, niacin 50mg/L, biotin 30mg/L, KNO ₃ 850mg/L, KH ₂ Soak in the root-promoting agent formed by the mixed preparation of PO ₄ 240mg/L for 4 hours, then use talcum powder to make the root-promoting agent into a slurry, dip the cuttings in the slurry and insert them directly into the prepared seedling substrate, and spray to moisturize to control seedling growth The relative humidity of the environment is 90-95%, the temperature is 20-30°C, and the light is 1500-2000lx. After about one month, new shoots will emerge from the top of the cuttings, and the root system will form. Rooted cuttings are obtained, and the rooting rate is 98.1%.

Move the rooted cuttings to the field nursery. In the first month, pay attention to shading, control the light at 1500-2000lx, the temperature at 20-30°C, and the relative humidity at 75-85%. Use a volume concentration of 1‰6N- Spray foliar fertilizer with 35P-10K high-phosphorus compound fertilizer; within the second month, pay attention to ventilation and moisturizing, with a relative humidity of 60-70%, and use a high-nitrogen compound fertilizer with a volume concentration of 1‰30N-13P-8K every 14 days Foliar fertilizer spraying was carried out; after 2 months, water, fertilizer and pest management were carried out according to conventional seedling raising methods, and the survival rate of masson pine seedlings was statistically 97.0%.

Example 3:

The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A 70-hole seedling tray with a hole size of 6 cm x 8 cm and serrated holes on the side of the hole is selected as a container for cutting seedlings. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising substrate with water, and use a mixture of volume concentration 0.2% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising substrate for later use. When cutting, the seedling-raising substrate in the seedling-raising bag The water content is controlled at 70%.

Select the newly pumped 3cm long, more than 4mm in diameter, initial lignification, soft and vigorous juvenile Pinus massoniana twigs with clusters of needles at the top as cuttings; the collected cuttings are quickly immersed in a volume concentration of 1.5% The cuttings are eluted and disinfected in the treatment solution obtained by mixing water ethanol + 0.1% malonic acid + 0.1% mancozeb + 0.02% streptomycin. Soak in the treatment solution for 10 minutes, then wash with clean water for later use.

Put the treated cuttings into a mixture containing NAA50mg/L, IBA50mg/L, ABT ₁ 50mg/L, 6-BA5mg/L, VB ₁ 50mg/L, VB ₆ 20mg/L, niacin 20mg/L, biotin 10mg/L L, KNO ₃ 850mg/L, KH ₂ PO ₄ 240mg/L mixed preparations, soak in the root accelerator for 12h, then use talcum powder to make the root accelerator into slurry, dip the cuttings in the slurry and insert them directly into the prepared In the seedling-growing medium, control the relative humidity of 90-95% by spraying and moisturizing, the temperature of 20-25°C, and the light of 1500-2000lx. After about 1 month, new shoots will emerge from the top of the cuttings, and the root system will form. Rooted cuttings are obtained, and the rooting rate is 98%.

Move the rooted cuttings to the field nursery. In the first month, pay attention to shading, control the light at 1500-2000lx, the temperature at 20-25°C, and the relative humidity at 75-85%. Use a volume concentration of 1‰6N- Spray foliar fertilizer with 35P-10K high-phosphorus compound fertilizer; within the second month, pay attention to ventilation and moisture, with a relative humidity of 60-70%, and use a volume concentration of 2‰30N-13P-8K high-nitrogen compound fertilizer every 10 days Spray foliage fertilizer; after 2 months, carry out water, fertilizer and pest management according to conventional seedling raising methods, and the survival rate of masson pine seedlings is 99.2%.

Example 4:

The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A 70-hole seedling tray with a hole size of 6 cm x 8 cm and serrated holes on the side of the hole is selected as a container for cutting seedlings. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising matrix with water, and use a mixture of volume concentration 0.1% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising matrix for later use. When cutting, the seedling-raising matrix in the seedling-raising bag The water content is controlled at 65%.

Select the newly pumped 3cm long, more than 4mm in diameter, initial lignification, soft and vigorous juvenile Pinus massoniana twigs with clusters of needles at the top as cuttings; the collected cuttings are quickly immersed in a volume concentration of 1.5% The cuttings are eluted and disinfected in the treatment solution obtained by mixing water ethanol + 0.1% malonic acid + 0.2% mancozeb + 0.02% streptomycin. Soak in the treatment solution for 5 minutes, then rinse with clean water for later use.

Put the treated cuttings into a mixture containing NAA100mg/L, IBA50mg/L, ABT ₁ 50mg/L, 6-BA10mg/L, VB ₁ 100mg/L, VB ₆ 50mg/L, niacin 50mg/L, biotin 30mg/L L, KNO ₃ 850mg/L, KH ₂ PO ₄ 240mg/L mixed preparations, soak in the root accelerator for 4 hours, then use talcum powder to make the root accelerator into slurry, dip the cuttings in the slurry and insert them directly into the prepared In the seedling-growing medium, control the relative humidity of 90-95% by spraying and moisturizing, the temperature of 25-30°C, and the light of 1500-2000lx. After about 1 month, new shoots will emerge from the top of the cuttings, and the root system will form. Rooted cuttings are obtained, and the rooting rate is 98.2%.

Move the rooted cuttings to the field nursery. In the first month, pay attention to shading, control the light at 1500-2000lx, the temperature at 25-30°C, and the relative humidity at 75-85%. Use a volume concentration of 1‰6N- Spray foliage fertilizer with 35P-10K high-phosphorus compound fertilizer; within the second month, pay attention to ventilation and moisturizing, with a relative humidity of 60-70%, and use a high-nitrogen compound fertilizer with a volume concentration of 2‰30N-13P-8K every 12 days Spray foliage fertilizer; after 2 months, carry out water, fertilizer and pest management according to the conventional seedling raising method. Statistics show that the survival rate of masson pine seedlings is 97.5%.

Example 5:

The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A seedling raising tray with 70 holes with a hole size of 6 cm x 8 cm and serrated holes on the side of the holes is selected as a container for cutting seedlings. Mix evenly with fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising substrate with water, and use a mixture of volume concentration 0.2% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising substrate for later use. When cutting, the seedling-raising substrate in the seedling-raising bag The water content is controlled at 70%.

Select the newly pumped 4cm long, more than 4mm in diameter, initial lignification, soft and vigorous juvenile Pinus massoniana twigs with clusters of needles at the top as cuttings; the collected cuttings are quickly immersed in a volume concentration of 1.5% The cuttings are eluted and disinfected in the treatment solution obtained by mixing water ethanol + 0.1% malonic acid + 0.1% mancozeb + 0.02% streptomycin. Soak in the treatment solution for 10 minutes, then wash with clean water for later use.

Put the treated cuttings into a mixture containing NAA50mg/L, IBA50mg/L, ABT ₁ 50mg/L, 6-BA10mg/L, VB ₁ 100mg/L, VB ₆ 30mg/L, niacin 30mg/L, biotin 20mg/L L, KNO ₃ 850mg/L, KH ₂ PO ₄ 240mg/L mixed preparations, soak in the root accelerator for 8 hours, then use talcum powder to make the root accelerator into slurry, dip the cuttings in the slurry and insert them directly into the prepared In the seedling-growing medium, control the relative humidity of 90-95% by spraying and moisturizing, the temperature of 20-30°C, and the light of 1500-2000lx. After about 1 month, new shoots will be drawn from the top of the cuttings, and the root system will be formed. Rooted cuttings will be obtained. The rooting rate is 99.4%.

Move the rooted cuttings to the field nursery. In the first month, pay attention to shading, control the light at 1500-2000lx, the temperature at 20-30°C, and the relative humidity at 75-85%. Use a volume concentration of 1‰6N- Spray foliage fertilizer with 35P-10K high-phosphorus compound fertilizer; in the second month, pay attention to ventilation and moisture, with a relative humidity of 60-70%, and use a volume concentration of 3‰30N-13P-8K high-nitrogen compound fertilizer every 14 days Spray foliage fertilizer; after 2 months, carry out water, fertilizer and pest management according to the conventional seedling raising method. Statistics show that the survival rate of masson pine seedlings is 98.9%.

Embodiment 6:

The invention discloses a method for raising seedlings of masson pine tissue-cultured seedlings with short branches and short spikes. A 70-hole seedling tray with a hole size of 6 cm x 8 cm and serrated holes on the side of the hole is selected as a container for cutting seedlings. Mix the fermented pine bark, coconut bran, yellow heart soil, and pumice in a volume ratio=2:2:4:2 to obtain a seedling-raising substrate, put the seedling-raising substrate into a non-woven fabric seedling-raising bag with a size of 8cm×9cm, and then place the In the seedling-raising tray, then drench the installed seedling-raising substrate with water, and use a mixture of volume concentration 0.3% carbendazim + volume concentration 0.5% potassium permanganate to sterilize the seedling-raising substrate for later use. When cutting, the seedling-raising substrate in the seedling-raising bag The water content is controlled at 75%.

Select the newly pumped 3cm long, more than 4mm in diameter, initial lignification, soft and vigorous juvenile Pinus massoniana twigs with clusters of needles at the top as cuttings; the collected cuttings are quickly immersed in a volume concentration of 1.5% The cuttings are eluted and disinfected in the treatment solution obtained by mixing water ethanol + 0.1% malonic acid + 0.2% mancozeb + 0.02% streptomycin. Soak in the treatment solution for 5 minutes, then rinse with clean water for later use.

Put the treated cuttings into a mixture containing NAA75mg/L, IBA25mg/L, ABT ₁ 25mg/L, 6-BA10mg/L, VB ₁ 75mg/L, VB ₆ 40mg/L, niacin 40mg/L, biotin 20mg/L L, KNO ₃ 850mg/L, KH ₂ PO ₄ 240mg/L mixed preparations, soak in the root accelerator for 10h, then use talcum powder to make the root accelerator into slurry, dip the cuttings in the slurry and insert them directly into the prepared In the seedling-growing medium, control the relative humidity of 90-95% by spraying and moisturizing, the temperature of 20-30°C, and the light of 1500-2000lx. After about 1 month, new shoots will be drawn from the top of the cuttings, and the root system will be formed. Rooted cuttings will be obtained. The rooting rate is 98.6%.

Move the rooted cuttings to the field nursery. In the first month, pay attention to shading, control the light at 1500-2000lx, the temperature at 20-30°C, and the relative humidity at 75-85%. Use a volume concentration of 1‰6N- Spray foliar fertilizer with 35P-10K high-phosphorus compound fertilizer; within the second month, pay attention to ventilation and moisturizing, with a relative humidity of 60-70%, and use high-nitrogen compound fertilizer with a volume concentration of 3‰30N-13P-8K every 12 days Spray foliage fertilizer; after 2 months, carry out water, fertilizer and pest management according to the conventional seedling raising method. Statistics show that the survival rate of masson pine seedlings is 97.5%.

Claims (6)

1. a masson pine plantlet in vitro spray single-node cutting seed-raising method, it is characterized in that: comprise selection of container, matrix preparation, cuttings collection, cuttings process, cuttage and nursery bed management operation, select suitable container for plant growth, according to a certain ratio and after sterilization method gets out matrix, juvenile form spray is collected as cuttings and carries out wash-out and disinfect, after rooting promoter process, directly carry out cuttage, period adds the control of the fertile condition of high light warm water, obtains Pinus massoniana Seedlings after carrying out nursery bed management; Its operating procedure is as follows:

(1) selection of container: select cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container;

(2) matrix prepares: non-woven fabrics Seedling bag seedling medium being loaded 8cm × 9cm size specification, then seedling-cultivating tray is placed in, water of then being drenched by the seedling medium installed, for subsequent use after sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is at 65-75%;

(3) cuttings collection: choose that newly to take out 3-4cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings;

(4) cuttings process: by the cuttings collected, immerses in treatment fluid rapidly, carries out the wash-out of cuttings and disinfect, soak time 5-10 minute, then using clear water cleaned standby seam;

(5) cuttage: the cuttings handled well is put into rooting promoter and soaks 4-12h, then use talcum powder rooting promoter furnishing pulpous state, in the seedling medium that after cuttings being dipped in slurry, directly inserting step (2) is got ready, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, and root growth, obtains cuttage seeding of taking root;

(6) nursery bed management: cuttage seeding of taking root is removed to nursery, land for growing field crops, controls light temperature water and fertilizer condition well in 1-2 month, after 2 months, seedling-cultivating method carries out liquid manure and pest management routinely.

2. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, it is characterized in that: the raw material of the seedling medium described in step (2) comprises fermented-pine bark, coconut palm chaff, yellow soil and float stone, and pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2 mix and obtain seedling medium.

3. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, is characterized in that: the sterilization described in step (2) is the mixed liquor adopting volumetric concentration 0.1-0.3% carbendazim+volumetric concentration 0.5% potassium permanganate.

4. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, is characterized in that: the treatment fluid described in step (4) is the mixed solution of volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.1-0.2% mancozeb+volumetric concentration 0.02% streptomycin.

5. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, is characterized in that: the rooting promoter described in step (5) is for containing: NAA50-100mg/L, IBA0-50mg/L, ABT ₁0-50mg/L, 6-BA5-10mg/L, VB ₁50-100mg/L, VB ₆20-50mg/L, nicotinic acid 20-50mg/L, vitamin h 10-30mg/L, KNO ₃850mg/L, KH ₂pO ₄the mix preparation of 240mg/L.

6. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, it is characterized in that: in the nursery bed management described in step (6), in 1st month, attention is shaded, illumination controls at 1500-2000lx, temperature at 20-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 7-10d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 10-14d volumetric concentration 1-3 ‰ 30N-13P-8K carries out Foliar Spraying.