CN105733927A - Consumable for nucleic acid extraction and multiple combined consumables - Google Patents
Consumable for nucleic acid extraction and multiple combined consumables Download PDFInfo
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- CN105733927A CN105733927A CN201610220035.5A CN201610220035A CN105733927A CN 105733927 A CN105733927 A CN 105733927A CN 201610220035 A CN201610220035 A CN 201610220035A CN 105733927 A CN105733927 A CN 105733927A
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- nucleic acid
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- acid extraction
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 61
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 61
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 61
- 238000000605 extraction Methods 0.000 title claims abstract description 41
- 238000010828 elution Methods 0.000 claims abstract description 26
- 239000008280 blood Substances 0.000 claims abstract description 20
- 210000004369 blood Anatomy 0.000 claims abstract description 20
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims description 64
- 238000005406 washing Methods 0.000 claims description 29
- 238000000926 separation method Methods 0.000 claims description 22
- 238000005336 cracking Methods 0.000 claims description 18
- 238000004140 cleaning Methods 0.000 abstract description 8
- 238000000354 decomposition reaction Methods 0.000 abstract 4
- 239000007788 liquid Substances 0.000 abstract 1
- 239000011324 bead Substances 0.000 description 18
- 230000005291 magnetic effect Effects 0.000 description 17
- 239000000523 sample Substances 0.000 description 10
- 239000006166 lysate Substances 0.000 description 6
- 239000003480 eluent Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229910052814 silicon oxide Inorganic materials 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
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- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
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- Molecular Biology (AREA)
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Abstract
The invention provides a consumable for nucleic acid extraction and multiple combined consumables.The consumable is used for containing reagents and a blood sample required by nucleic acid extraction and comprises a splitting decomposition container, a cleaning container and an elution container which are arranged linearly and form an integral structure.The splitting decomposition container, the cleaning container and the elution container are respectively used for containing a splitting decomposition liquid, a blood sample, a cleaning solution and an eluant, the integral consumable is not needed to be frequently replaced when being not used, and one consumable can complete splitting decomposition, cleaning, elution and other steps, facilitates usage and accordingly improves the nucleic acid extraction efficiency.The multiple combined consumables are formed by a plurality of the integral consumables side by side and facilitate simultaneous nucleic acid extraction of multiple blood samples.
Description
Technical field
The present invention relates to biological technical field, more particularly, to a kind of consumptive material for nucleic acid extraction and multi-joint consumptive material.
Background technology
Nucleic acid includes two kinds of molecules of DNA, RNA, the biotechnology being object of study with nucleic acid, including to a series of technology such as the extraction of nucleic acid, clone, amplification, detection, order-checkings, develop rapidly in recent years, these technology not only use in research unit at present, and multiple functional departments extensive use in social life.And the first step of nucleic acids research, it is simply that extracting nucleic acid from various biological samples, the efficiency of nucleic acid extraction becomes the restraining factors of downstream nucleic acid research success.Nucleic acid is all exist with the state with protein bound in cell, mainly comprising the following steps of nucleic acid extraction: the impurity such as cell lysis removes the protein and the biomacromolecule such as polysaccharide, lipid that are combined with nucleic acid purification of nucleic acid, removes salt, organic agent.
Existing nucleic acid extraction adopts paramagnetic particle method substantially, uses nanotechnology that the surface of superparamagnetic nano particle is improved and after finishing, prepares into superparamagnetism silicon oxide nanometer magnetic bead.This magnetic bead can identify specifically with nucleic acid molecules on micro interface and efficiently be combined.Utilize the superparamagnetism of silicon oxide Nano microsphere, under the effect of Chaotropic salt (guanidine hydrochloride, guanidinium isothiocyanate etc.) and externally-applied magnetic field, can separate by DNA and RNA from blood, animal tissue, food, pathogenic microorganism equal samples, can be applicable to clinical disease diagnosis, transfusion safety, Forensic Identification, disease control detection, nucleic acid samples build the multiple fields such as storehouse, environmental microorganism detection, food safety detection, molecular biology research.
Existing nucleic acid-extracting apparatus, its reaction consumptive material is for containing the required lysate of reaction, cleaning mixture and eluent, and different steps needs to adopt different reaction consumptive materials, uses inconvenience, causes nucleic acid extraction efficiency low.
Summary of the invention
It is contemplated that solve above-mentioned technical problem at least to a certain extent.
The primary and foremost purpose of the present invention is to overcome the awkward defect described in above-mentioned prior art, it is provided that a kind of consumptive material for nucleic acid extraction easy to use.
A further object of the present invention is to provide a kind of multi-joint consumptive material for nucleic acid extraction easy to use.
For solving above-mentioned technical problem, technical scheme is as follows:
For solving above-mentioned technical problem, technical scheme is as follows:
A kind of consumptive material for nucleic acid extraction, is used for containing the reagent needed for nucleic acid extraction and blood sample, and described consumptive material includes the cracking container, washing container and the elution container that are arranged in a linear, and described cracking container, washing container and elution container are integral type structure.
In the preferred scheme of one, the number of described washing container is multiple, and it is arranged in a linear.The magnetic bead that multiple washing containers are easy to being adsorbed with nucleic acid repeatedly washs, to reduce impurity content as far as possible.
In the preferred scheme of one, described consumptive material also includes the separation sleeve container for holding separation sleeve.
In the preferred scheme of one, described consumptive material also includes eluting pipe, and eluting pipe is placed in elution container.Eluting pipe is separable with elution container, has extracted rear nucleic acid and has existed and in eluting pipe, when the nucleic acid extracted is detected further, taken out by eluting pipe from elution container, without mobile whole consumptive material.
A kind of multi-joint consumptive material for nucleic acid extraction, described multi-joint consumptive material is formed side by side by some above-mentioned consumptive material integral types.
The present invention is in use, cracking container adds lysate and magnetic bead, nucleic acid-extracting apparatus takes out separation sleeve by gear shaft from separation sleeve container, separation sleeve is socketed in gear shaft bottom, separation sleeve is moved in cracking container by gear shaft, gear shaft drives separation sleeve to be stirred, and after blood sample cracking, nucleic acid absorption is on magnetic bead, and namely the core of gear shaft is absorption magnetic bead in the set tip of separation sleeve after inserting bar magnet；Magnetic bead is transferred to successively in the first washing container, the second washing container, the 3rd washing container and wash；Finally magnetic bead is transferred in the first elution container or eluting pipe to carry out eluting be nucleic acid and Beads enrichment, the extraction of nucleic acid can be completed.
Compared with prior art, technical solution of the present invention provides the benefit that: the present invention provides a kind of consumptive material for nucleic acid extraction, for containing reagent needed for nucleic acid extraction and blood sample, described consumptive material includes the cracking container, washing container and the elution container that are arranged in a linear, and described cracking container, washing container and elution container are integral type structure.Cracking container, washing container and elution container are respectively used to contain lysate and blood sample, cleaning mixture and eluent, the consumptive material of integral type need not frequently change consumptive material in use, one piece of consumptive material can complete the steps such as cracking, washing, eluting, it is simple to uses, thus improve the efficiency of nucleic acid extraction.
The present invention also provides for a kind of multi-joint consumptive material for nucleic acid extraction, and multi-joint consumptive material is formed side by side by some above-mentioned consumptive material integral types, and multi-joint consumptive material is easy to multiple blood samples be carried out nucleic acid extraction simultaneously.
Accompanying drawing explanation
Fig. 1 is the present invention structural representation for the consumptive material of nucleic acid extraction.
Fig. 2 is the schematic diagram of tetrad consumptive material in embodiment 2.
Wherein: 1, cracking container；2, separation sleeve container；3, the first washing container；4, the second washing container；5, the 3rd washing container；6, the first elution container；7, the second elution container；8, separation sleeve；9, eluting pipe.
Detailed description of the invention
Accompanying drawing being merely cited for property explanation, it is impossible to be interpreted as the restriction to this patent；
In order to the present embodiment is better described, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product；
To those skilled in the art, in accompanying drawing, some known features and explanation thereof are likely to omission and will be understood by.
Below in conjunction with drawings and Examples, technical scheme is described further.
Embodiment 1
As shown in Figure 1, a kind of consumptive material for nucleic acid extraction, for containing reagent needed for nucleic acid extraction and blood sample, described consumptive material includes the cracking container 1, separation sleeve container the 2, first washing container the 3, second washing container the 4, the 3rd washing container the 5, first elution container 6 and the second elution container 7 that are arranged in a linear, and above-mentioned each container is integral type structure.
Cracking container 1 is used for holding lysate and blood sample, and separation sleeve container 2 is used for holding separation sleeve 8.
First washing container the 3, second washing container 4 and the 3rd washing container 5 are all used for holding cleaning mixture, and the magnetic bead that multiple washing containers are easy to being adsorbed with nucleic acid repeatedly washs, to reduce impurity content as far as possible.
First elution container 6 and the second elution container 7 be optional one in use, when selecting the first elution container 6, it is necessary to add eluent wherein；When selecting the second elution container 7, put into eluting pipe 9 wherein, eluting pipe 9 adds eluent, eluting pipe 9 and the second elution container 7 are separable, after extraction completes, nucleic acid exists and in eluting pipe 9, when the nucleic acid extracted is detected further, eluting pipe 9 is taken out from the second elution container 7, without mobile whole consumptive material.
The present invention is in use, cracking container 1 adds lysate and magnetic bead, nucleic acid-extracting apparatus takes out separation sleeve 8 by gear shaft from separation sleeve container 2, separation sleeve 8 is socketed in gear shaft bottom, separation sleeve 8 is moved in cracking container 1 by gear shaft, gear shaft drives separation sleeve 8 to be stirred, and after blood sample cracking, nucleic acid absorption is on magnetic bead, and namely the core of gear shaft is absorption magnetic bead in the set tip of separation sleeve 8 after inserting bar magnet；Magnetic bead is transferred to successively in first washing container the 3, second washing container the 4, the 3rd washing container 5 and wash；Finally magnetic bead is transferred in the first elution container 6 or eluting pipe 9 to carry out eluting be nucleic acid and Beads enrichment, the extraction of nucleic acid can be completed.
The present invention provides a kind of consumptive material for nucleic acid extraction, for containing reagent needed for nucleic acid extraction and blood sample, the consumptive material of integral type need not frequently change consumptive material in use, and one piece of consumptive material can complete the steps such as cracking, washing, eluting, easy to use, thus improve the efficiency of nucleic acid extraction.
Embodiment 2
The present embodiment provides a kind of multi-joint consumptive material for nucleic acid extraction, and multi-joint consumptive material consumptive material integral type described in some embodiments 1 forms side by side, and multi-joint consumptive material is easy to multiple blood samples be carried out nucleic acid extraction simultaneously.Fig. 2 is the schematic diagram of tetrad consumptive material, and tetrad consumptive material forms side by side by adopting 4 above-mentioned consumptive material integral types.
Multi-joint consumptive material is easy to multiple blood samples be carried out nucleic acid extraction simultaneously, in actual applications, extracts the blood sample number of nucleic acid as required, multi-joint consumptive material is combined.Such as, extract nucleic acid if desired for 6 blood samples, then adopt 1 tetrad consumptive material and 2 common consumptive materials side by side；Extract nucleic acid if desired for 8 blood samples, then adopt 2 tetrad consumptive materials side by side, the so not only waste that it also avoid consumptive material easy to use.
Common consumptive material and multi-joint consumptive material all can pre-packaged reagent, it is previously added in consumptive material by lysate, cleaning mixture, eluent, magnetic bead and separation sleeve, eluting pipe 9 can be put and also can not be placed in consumptive material, by the mode of heating, aluminum film is sealed up whole container mouths of pipe afterwards, during use, aluminum film is torn or pierces through, can test, reduce toward the loading time in consumptive material, improve extraction efficiency.When adopting pre-packaged, any number of blood sample is all without waste reagent and consumptive material, and this is also the bright spot of this consumptive material.During pre-packaged reagent, magnetic bead can leave in arbitrary hole of three washing containers 602.
The corresponding same or analogous parts of same or analogous label；
Term the being merely cited for property explanation of position relationship described in accompanying drawing, it is impossible to be interpreted as the restriction to this patent；
Obviously, the above embodiment of the present invention is only for clearly demonstrating example of the present invention, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.All any amendment, equivalent replacement and improvement etc. made within the spirit and principles in the present invention, should be included within the protection domain of the claims in the present invention.
Claims (5)
1. the consumptive material for nucleic acid extraction, for containing reagent needed for nucleic acid extraction and blood sample, it is characterized in that, described consumptive material includes the cracking container, washing container and the elution container that are arranged in a linear, and described cracking container, washing container and elution container are integral type structure.
2. the consumptive material for nucleic acid extraction according to claim 1, it is characterised in that the number of described washing container is multiple, and it is arranged in a linear.
3. the consumptive material for nucleic acid extraction according to claim 1, it is characterised in that described consumptive material also includes the separation sleeve container for holding separation sleeve.
4. the consumptive material for nucleic acid extraction according to claim 1, it is characterised in that described consumptive material also includes eluting pipe, and eluting pipe is placed in elution container.
5. the multi-joint consumptive material for nucleic acid extraction, it is characterised in that described multi-joint consumptive material consumptive material integral type described in some claim 1 forms side by side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610220035.5A CN105733927A (en) | 2016-04-11 | 2016-04-11 | Consumable for nucleic acid extraction and multiple combined consumables |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610220035.5A CN105733927A (en) | 2016-04-11 | 2016-04-11 | Consumable for nucleic acid extraction and multiple combined consumables |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105733927A true CN105733927A (en) | 2016-07-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201610220035.5A Pending CN105733927A (en) | 2016-04-11 | 2016-04-11 | Consumable for nucleic acid extraction and multiple combined consumables |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108517290A (en) * | 2018-05-31 | 2018-09-11 | 西安交通大学 | A kind of five chamber nucleic acid-extracting apparatus and method based on immiscible phase interfacial tension |
| CN110819627A (en) * | 2019-11-29 | 2020-02-21 | 杭州博日科技有限公司 | Multi-column single tube consumable and use method thereof in nucleic acid extraction and purification by magnetic rod method |
| CN112126589A (en) * | 2020-10-13 | 2020-12-25 | 南京中科拜尔医学技术有限公司 | Autonomous liquid suction type nucleic acid detection card box based on magnetic rod method and use method |
| CN112340686A (en) * | 2020-04-26 | 2021-02-09 | 杭州博日科技股份有限公司 | Full-automatic liquid split charging equipment |
| CN112442443A (en) * | 2020-06-11 | 2021-03-05 | 杭州天微基因科技有限公司 | Nucleic acid extraction and amplification integrated detection method and detection connecting pipe |
| CN112852626A (en) * | 2021-03-22 | 2021-05-28 | 广州滴纳生物科技有限公司 | Integrated sample detection device and detection method thereof |
| CN112920932A (en) * | 2021-03-17 | 2021-06-08 | 杭州博日科技股份有限公司 | Consumable fixing device for nucleic acid extraction and using method thereof |
| CN114606111A (en) * | 2021-12-27 | 2022-06-10 | 宁波爱基因科技有限公司 | Nucleic acid extraction tube structure, magnetic rod sleeve and nucleic acid extraction method |
| WO2023168760A1 (en) * | 2022-03-10 | 2023-09-14 | 广东和信健康科技有限公司 | Detection consumable and detection apparatus |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108517290A (en) * | 2018-05-31 | 2018-09-11 | 西安交通大学 | A kind of five chamber nucleic acid-extracting apparatus and method based on immiscible phase interfacial tension |
| CN110819627A (en) * | 2019-11-29 | 2020-02-21 | 杭州博日科技有限公司 | Multi-column single tube consumable and use method thereof in nucleic acid extraction and purification by magnetic rod method |
| CN112340686A (en) * | 2020-04-26 | 2021-02-09 | 杭州博日科技股份有限公司 | Full-automatic liquid split charging equipment |
| CN112442443A (en) * | 2020-06-11 | 2021-03-05 | 杭州天微基因科技有限公司 | Nucleic acid extraction and amplification integrated detection method and detection connecting pipe |
| CN112442443B (en) * | 2020-06-11 | 2023-07-28 | 杭州天微基因科技有限公司 | Nucleic acid extraction and amplification integrated detection method and detection connecting tube |
| CN112126589A (en) * | 2020-10-13 | 2020-12-25 | 南京中科拜尔医学技术有限公司 | Autonomous liquid suction type nucleic acid detection card box based on magnetic rod method and use method |
| CN112920932A (en) * | 2021-03-17 | 2021-06-08 | 杭州博日科技股份有限公司 | Consumable fixing device for nucleic acid extraction and using method thereof |
| CN112920932B (en) * | 2021-03-17 | 2023-10-27 | 杭州博日科技股份有限公司 | Consumable fixing device for nucleic acid extraction and use method thereof |
| CN112852626A (en) * | 2021-03-22 | 2021-05-28 | 广州滴纳生物科技有限公司 | Integrated sample detection device and detection method thereof |
| CN114606111A (en) * | 2021-12-27 | 2022-06-10 | 宁波爱基因科技有限公司 | Nucleic acid extraction tube structure, magnetic rod sleeve and nucleic acid extraction method |
| WO2023168760A1 (en) * | 2022-03-10 | 2023-09-14 | 广东和信健康科技有限公司 | Detection consumable and detection apparatus |
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