CN105981908B - Liquid dairy product added with bovine colostrum and preparation method thereof - Google Patents
Liquid dairy product added with bovine colostrum and preparation method thereof Download PDFInfo
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- CN105981908B CN105981908B CN201510039406.5A CN201510039406A CN105981908B CN 105981908 B CN105981908 B CN 105981908B CN 201510039406 A CN201510039406 A CN 201510039406A CN 105981908 B CN105981908 B CN 105981908B
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- bovine colostrum
- sterile
- dairy product
- liquid dairy
- solution
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Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Dairy Products (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides a liquid dairy product added with bovine colostrum and a preparation method thereof. The liquid dairy product comprises 5-20% of sterile bovine colostrum solution, wherein the sterile bovine colostrum solution is obtained by degreasing raw material liquid bovine colostrum, removing part of casein and then carrying out microfiltration. The liquid dairy product can be a neutral liquid dairy product or an acidic liquid dairy product. The invention can avoid the denaturation inactivation of the immunoglobulin in the bovine colostrum caused by high-temperature sterilization in the processing process, well ensures the content of the immunoglobulin, and adds the bovine colostrum component which is sterile and has active IgG in the liquid dairy product, thereby greatly improving the immunological properties of the liquid dairy product.
Description
Technical Field
The invention relates to a liquid dairy product added with bovine colostrum and a preparation method thereof, in particular to a technology for preparing a sterile liquid bovine colostrum solution by utilizing a polypropylene filter membrane and adding the sterile liquid bovine colostrum solution into the liquid dairy product, and belongs to the field of dairy product preparation.
Background
The bovine colostrum is milk secreted by healthy cows within 72 hours after farrowing, contains precious active immunoglobulin and abundant nutritional ingredients such as milk calcium, protein, various trace elements and the like, and is known as the king of immunity in the 21 st century. Compared with normal milk, the colostrum milk has the advantages that the content is obviously different, the protein content of colostrum is higher, the fat and sugar content is lower, the iron content is 10-17 times of that of the normal milk, and the vitamin D and the vitamin A are 3 times and 10 times of that of the normal milk respectively.
The colostrum of cow has attracted much attention because it naturally contains various immune factors, such as active immunoglobulin, lactoferrin, lactoperoxidase, lysozyme, etc., of which the most important substance is active immunoglobulin, and the content of which is more than 10 times that of colostrum. The bovine colostrum contains five types of immunoglobulin, namely IgA, IgD, IgE, IgM and IgG, wherein the content of the IgG is more than 80%, and the characteristics of the immunoglobulin are more stable than those of other four types of immunoglobulin, and the immunoglobulin is the most main functional substance. A large number of researches and clinical trials prove that IgG in the bovine colostrum has the immunoregulation function, can effectively prevent and improve diseases, enhances the disease resistance, and can inhibit various pathogenic microorganisms. In addition, the bovine colostrum has other functions, such as promoting growth and development, improving intelligence, improving body condition, improving athletic performance, relieving fatigue, delaying aging, regulating intestinal flora, etc. Bovine colostrum was listed as the most promising non-herbal natural health food in the 21 st century by the American food technology Association (IFT) in 2000.
However, bovine colostrum contains some sources of sensitization, especially if applied to infant food, with a large potential risk. A great deal of research at present shows that the sensitization source is mainly in casein, so that the sensitization risk can be greatly reduced if the casein in the bovine colostrum is removed. On the other hand, since IgG in bovine colostrum is a strong heat-sensitive substance, the inactivation rate can reach 95% when the temperature is kept at 40 ℃ for 30min, and therefore, the conventional high-temperature sterilization technology cannot be used for sterilization. These factors all affect the use of bovine colostrum to some extent. Therefore, the method is important to ensure the sterility of the bovine colostrum on the premise of eliminating the potential sensitization source of the bovine colostrum and ensuring the activity of IgG.
For the solution containing heat-sensitive substances, the membrane filtration is a good sterilization process, particularly static filtration (parallel flow filtration), the temperature rise cannot be caused in the membrane filtration process, the heat-sensitive substances cannot be damaged, meanwhile, the particle size of microorganisms in the solution is larger than 0.2 μm, and the complete sterilization effect can be achieved when the microorganisms pass through a 0.2 μm microfiltration membrane. However, the bovine colostrum solution is very viscous and contains more substances with larger particle size, and if the bovine colostrum solution is directly passed through a membrane, the membrane flux is very low.
In the prior art, research reports on sterilization of bovine colostrum by adopting a microfiltration membrane filtration technology have been reported, wherein most of the microfiltration membranes are ceramic membranes. For example, CN101263839A discloses that milk containing bovine colostrum powder is filtered and sterilized by a ceramic microfilm with a pore diameter of 0.8-1.4 microns. The literature, "analysis of pollution resistance during the filtration of bovine colostrum by ceramic membrane" (Wangzhigao, Chen Wei hong, Xunan Ping, membrane science and technology, 2.2004, vol. 24, No. 1) examines the influence of pore size on the process of membrane filtration of bovine colostrum, and analyzes the formation of membrane filtration resistance with pore size of 0.2 μm, 0.5 μm and 0.8 μm and the influence thereof on permeation flux and protein retention rate. The ceramic filter membrane is prepared from materials such as alumina, titanium oxide, zirconia and the like through a special process, has high mechanical strength and strong pressure resistance, however, when the ceramic filter membrane is used for microfiltration, the operation mode is usually cross flow operation (namely dynamic filtration), has high tangential flow velocity, and can enable bovine colostrum to better pass under the conditions of high pressure and high rotating speed, thereby achieving the membrane-passing effect. But higher temperatures are generated during the filtration process, which causes denaturation and inactivation of IgG in the bovine colostrum; meanwhile, the ceramic membrane has high cost and needs other supporting facilities, so that the overall cost is too high during production, and the ceramic membrane is not beneficial to popularization.
CN 103039630A discloses a method for preparing sterile bovine colostrum whey with natural immunocompetence by a membrane filtration technology, wherein fat is removed from bovine colostrum by centrifugation, casein is precipitated by adopting an isoelectric point method or a chymosin method, obtained whey liquid is pre-filtered by adopting a hollow fiber membrane or a ceramic membrane with the aperture of 0.4-1.2 mu m, and residual fat, casein and most of thalli in the whey are removed; and filtering and sterilizing by adopting a polytetrafluoroethylene membrane filter with the pore diameter of 0.1-0.4 mu m. By using the method, the treatment capacity of the feed liquid is improved, and the loss rate of IgG can be reduced to a certain degree. However, the prefiltration in this method also employs dynamic filtration, as mentioned above, where the tangential flow rate allows the bovine colostrum to pass through the filter membrane well, but during the filtration process the temperature rises causing denaturation inactivation of IgG in the bovine colostrum. In addition, the method needs to adopt two membranes made of different materials to filter the bovine colostrum, the number of matched filter equipment is large, and the prices of the hollow fiber membrane, the ceramic membrane and the polytetrafluoroethylene membrane are relatively high, so that the practical application of the technology is limited.
Furthermore, the literature "influence of pH on the isolation and purification of insulin-like growth factor-1 (IGF-1) from bovine colostrum" (Xiaoyan, journal of food and Biotechnology, 7.2005, Vol. 24, No. 4) showed that IGF-1 permeability increased under alkaline conditions during ultrafiltration. It is believed that at pH >7, whey proteins acquire a net negative charge, increasing the electrostatic repulsion between molecules, eliminating the aggregation of protein molecules and increasing the permeability. However, the reference does not specify the filter material used.
On the other hand, milk is one of the favorite foods in daily life, and is called the most 'nearly perfect food' due to rich nutrition, easy digestion and absorption and convenient eating. If the bovine colostrum can be added into milk to develop a new liquid dairy product, the immunity of the milk can be greatly improved, and the nutrition of the milk is more comprehensive. However, due to the above-mentioned deficiencies in the art regarding the production of sterile bovine colostrum, which is free of allergens and ensures IgG activity, its practical application in liquid dairy products to which components of bovine colostrum are added is limited. In addition, because the components of the treated bovine colostrum are different from those of common milk, how to ensure the stability and good flavor and taste of the bovine colostrum after the bovine colostrum is added into the milk is also a problem to be considered.
Disclosure of Invention
The invention aims to develop a liquid dairy product added with bovine colostrum, wherein the bovine colostrum component which is sterile and has active IgG is added into the liquid dairy product, so that the immunological properties of the liquid dairy product are greatly improved.
The invention also aims to provide a preparation method of the liquid dairy product added with the bovine colostrum.
In order to provide a liquid dairy product added with bovine colostrum, the inventor of the invention starts with the research of preparing the sterile bovine colostrum rich in IgG, and finds in research that a polypropylene microfiltration membrane is a commonly used filter membrane in the food industry, takes food-grade isotactic polypropylene as a raw material, and has no additive in the whole production process; the physical and chemical properties are stable, and the compatibility is good; has a series of apertures, high porosity, large pollutant-receiving capacity, capability of back flushing and high-temperature disinfection and low price. Polypropylene filters have been widely used for micro-filtration sterilization in the food industry, however, no report on the use of polypropylene micro-filtration membranes for sterilization of bovine colostrum has been found in the prior art. The main reason for this may be that the mechanical strength of polypropylene microfiltration membranes widely available on the market is much lower than that of ceramic membranes, in particular, polypropylene microfiltration membranes have high low temperature brittleness and a pressure resistance range of not higher than 3Bar, and if they are used for static filtration of bovine colostrum, even if the bovine colostrum is degreased and casein-removed in advance, the emulsion after degreasing and casein-removal is difficult to pass through the polypropylene microfiltration membrane smoothly under a low pressure, and the membrane flux is low. After further research, the inventor of the present invention found that, by controlling the casein content in the defatted bovine colostrum emulsion and adjusting the emulsion to a specific pH value, the membrane flux can be significantly increased, so that the bovine colostrum emulsion can smoothly pass through a 0.2 μm polypropylene filter membrane in a static filtration manner under a relatively low pressure. The above operation is carried out in a sterile environment to obtain a sterile bovine colostrum solution, which can avoid denaturation and inactivation of immunoglobulin (mainly IgG) due to high temperature. Therefore, the invention successfully adopts the polypropylene filter membrane to carry out microfiltration on the bovine colostrum to prepare the sterile liquid bovine colostrum solution, can reduce the loss of IgG in the processing process, and takes the obtained sterile liquid bovine colostrum as one of the raw materials to prepare the liquid dairy product added with the bovine colostrum.
Specifically, in one aspect, the invention provides a liquid dairy product added with bovine colostrum, and the ingredients of the liquid dairy product comprise 5-20% of sterile bovine colostrum solution based on the total weight of the liquid dairy product, wherein the sterile bovine colostrum solution is obtained by degreasing raw material liquid bovine colostrum, removing part of casein, and then carrying out microfiltration.
As mentioned above, the obtaining of the sterile bovine colostrum which can eliminate the potential sensitization source of the bovine colostrum and ensure the IgG activity is one of the keys that the liquid dairy product added with the bovine colostrum and the preparation method thereof can be practically popularized and applied. According to a particular embodiment of the invention, the sterile bovine colostrum solution used in the present invention is prepared according to the following method:
degreasing raw material liquid bovine colostrum;
removing part of casein from the degreased emulsion and controlling the content of the casein in the emulsion to be 10-20% of the total content of protein in the emulsion;
and (3) adjusting the pH value of the emulsion after removing part of casein to 4.5-5.5 by using weak alkaline salt, and sequentially carrying out microfiltration by using polypropylene filter membranes of 0.8 mu m, 0.45 mu m and 0.2 mu m to obtain filtrate, namely the sterile liquid bovine colostrum solution.
According to the specific embodiment of the invention, the microfiltration mode of operation of the sterile liquid bovine colostrum solution is static filtration, namely parallel flow filtration, and temperature rise cannot be caused and heat-sensitive substances cannot be damaged in the membrane filtration process.
According to a specific embodiment of the present invention, in the preparation process of the sterile liquid bovine colostrum solution, the raw material liquid bovine colostrum is raw fresh bovine colostrum or reconstituted milk reconstituted from bovine colostrum powder. The content of IgG in the liquid bovine colostrum as the raw material is usually 10-100 mg/mL, generally 10-50 mg/mL, and is different according to the collection time and batch of the bovine colostrum. Preferably, the raw material liquid bovine colostrum has the total solid content of 13.0-18.0%, the fat content of 3.0-5.0%, the total protein content of 4.0-6.0% and the IgG content of 10-50 mg/mL.
According to a specific embodiment of the present invention, the preparation process of the sterile liquid bovine colostrum solution can be a degreasing process that can completely remove fat in the bovine colostrum by using a conventional degreasing process in the field.
According to a specific embodiment of the present invention, in the preparation process of the sterile liquid bovine colostrum solution, the process of removing part of the casein comprises adjusting the pH of the defatted emulsion to 4.3-4.6 by using an acid precipitation method, precipitating the casein, and then filtering the casein by filter bag filtration (preferably using a filter bag with 140-180 meshes) and/or centrifugation (for example, centrifugation at 3000 rpm-4000 rpm and at a low temperature, for example, 4-10 ℃ for 20 min-30 min). Because the isoelectric point of the immunoglobulin IgG in the bovine colostrum is slightly basic, the content of the immunoglobulin IgG is not influenced when the immunoglobulin IgG is treated by an acid precipitation method, and compared with other methods for precipitating casein, the method has the advantages of lower cost, simpler process and convenience for actual production operation. The acid used in the acid precipitation method can be one or more of lactic acid, acetic acid and citric acid. In the invention, complete removal of casein is not pursued, but the content of casein in the filtrate is controlled to be 10-20 wt% of the total content of protein in the emulsion. Unless otherwise indicated, all percentages and ratios recited herein are by weight.
According to a specific embodiment of the invention, the weakly basic salt used for adjusting the pH value in the preparation process of the sterile liquid bovine colostrum solution is selected from one or more of sodium bicarbonate, potassium bicarbonate, sodium acetate and potassium acetate, and preferably sodium bicarbonate. In specific application, the pH value is adjusted by using a weak alkaline saline solution with the concentration of 5-15%, preferably 5-10%. The use of these weakly basic salts can minimize the loss of IgG content due to precipitation or denaturation inactivation of IgG caused by local overbasing, and can not bring significant adverse effects on the membrane-passing effect.
According to the specific embodiment of the invention, in the preparation process of the sterile liquid bovine colostrum solution, the membrane-passing pressure difference of each membrane is controlled to be 0.5-2 Bar during microfiltration, namely, the emulsion after the pH value is adjusted sequentially passes through 0.8-0.45-0.2-micron filter membranes under the pressure of 0.5-2 Bar (the pressure difference of two sides of the membrane). In the filtering process, the membrane-passing pressure difference of each membrane can properly float within the range of 0.5-2 Bar, but cannot exceed the pressure-bearing range of the polypropylene filter membrane.
In the present invention, there is no special requirement for the microfiltration device, for example, a plate and frame filter commonly used in industry can be used, and other microfiltration equipment capable of realizing a pressure difference static filtration operation mode can also be used.
It will be appreciated that in the process for the preparation of a sterile liquid bovine colostrum solution according to the invention, the steps should be operated at a relatively low temperature to avoid loss of active substance from the emulsion due to excessive temperatures. Preferably, the temperature of the emulsion in the whole process is controlled to be not higher than 10 ℃, and more preferably 4-8 ℃.
The sterile liquid bovine colostrum solution prepared by the method has the IgG content of 10-100 mg/mL, and usually 10-50 mg/mL. Although the sterile liquid bovine colostrum solution according to the invention retains a certain amount of casein in the emulsion before membrane filtration during the preparation process, it was experimentally confirmed that no allergenic substances were present in the sterile liquid bovine colostrum solution obtained after filtration. The technology of the invention can remove the possible allergic substances in the bovine colostrum and greatly reduce the potential risk while enabling the emulsion to smoothly pass through the polypropylene filter membrane in a static filtering mode, and well ensures the content of the functional components in the bovine colostrum, particularly the content of immunoglobulin IgG.
In the invention, the sterile liquid bovine colostrum solution is further taken as one of the raw materials to be added into the liquid dairy product, so as to prepare the liquid dairy product added with the bovine colostrum. In the liquid dairy product added with the bovine colostrum, other components except the added sterile liquid bovine colostrum solution can be carried out according to the conventional operation in the field.
According to the specific embodiment of the invention, the liquid dairy product added with bovine colostrum can be a neutral liquid dairy product or an acidic liquid dairy product.
In a preferred embodiment of the present invention, the liquid dairy product added with bovine colostrum is a neutral liquid dairy product, and the ingredients of the neutral liquid dairy product comprise, based on the total weight of the liquid dairy product: 5 to 20 percent of sterile bovine colostrum solution, 0.8 to 2.5 percent of stabilizer, 0.05 to 0.25 percent of sweetener and the balance of milk.
In another preferred embodiment of the present invention, the liquid dairy product added with bovine colostrum is an acidic liquid dairy product, and the ingredients of the acidic liquid dairy product comprise, based on the total weight of the liquid dairy product: 5 to 20 percent of sterile bovine colostrum solution, 0.3 to 0.8 percent of stabilizer, 0.05 to 0.25 percent of sweetener, 0.05 to 0.5 percent of acidity regulator and the balance of milk.
It is understood that in the formulation of the liquid dairy product with added bovine colostrum of the present invention, the content of the sterile bovine colostrum solution can be adjusted appropriately according to the content of IgG in the solution.
According to the specific embodiment of the present invention, the sweetener and the stabilizer in the ingredients of the liquid dairy product with bovine colostrum can be selected from those commonly used in the art. Preferably, in order to increase the sweetness and mouthfeel of the liquid dairy product to which the bovine colostrum is added, the sweetener of the present invention is preferably one or more of neotame, aspartame, sodium cyclamate and acesulfame potassium. In order to further improve the stability of the neutral liquid dairy product added with the bovine colostrum, the stabilizer is preferably one or more of gellan gum, carrageenan, xanthan gum and microcrystalline cellulose. In order to further improve the stability of the acidic liquid dairy product added with the bovine colostrum, the used stabilizer is a thickening agent and/or an emulsifying agent, and the thickening agent is preferably one or more of carboxymethyl cellulose, pectin, Arabic gum, carrageenan, guar gum and modified starch; the emulsifier is preferably one or more of glyceryl monostearate, sucrose fatty acid glyceride, sodium stearoyl lactylate and glyceryl distearate. The specific amounts of sweetener and stabilizer may be determined by reference to the state of the art or by simple experimentation.
According to a specific embodiment of the invention, the acidity regulator in the ingredient of the acidic liquid dairy product with bovine colostrum is preferably one or more of lactic acid, acetic acid, citric acid and sodium citrate. The specific dosage of the water-soluble dairy product is determined according to the need of adjusting the liquid dairy product to a proper pH value (usually the pH value is 4.2-4.4).
According to the specific embodiment of the invention, the milk used in the ingredients of the invention meets the requirements of conventional raw milk, wherein the protein is more than or equal to 3.0(g/100g), the dry matter is more than or equal to 12.5(g/100g), and the rest part meets the requirements of GB 19301-. Specifically, the milk is full-cream milk, skim milk or milk with the fat content of 2.0(g/100g) to 3.0(g/100 g).
On the other hand, the invention also provides a preparation method of the liquid dairy product added with the bovine colostrum, which comprises the following steps:
mixing the liquid dairy product ingredients except the sterile bovine colostrum solution, homogenizing, sterilizing, and cooling to below 25 deg.C to obtain milk base material;
and adding the sterile bovine colostrum solution into the cooled milk base material in an online adding manner to prepare the liquid dairy product added with the bovine colostrum.
According to the specific embodiment of the invention, the preparation method of the liquid dairy product added with bovine colostrum further comprises the process of degreasing the raw material liquid bovine colostrum, removing part of casein, and then carrying out microfiltration to obtain sterile bovine colostrum solution. More specific preparation of sterile bovine colostrum solution is described above.
In the method for preparing the liquid dairy product added with bovine colostrum, the steps which are not mentioned in detail, such as the specific batching process of the milk base material (including homogenization, sterilization, acid adjustment and the like), the online adding process and the like, can be carried out according to the conventional operation in the field.
In conclusion, by applying the technology provided by the invention, the prepared liquid bovine colostrum solution can achieve a sterile effect, and simultaneously, the active immune substance IgG in the bovine colostrum can be well preserved.
Detailed Description
For a more clear understanding of the technical features, objects and advantages of the present invention, reference is now made to the following detailed description of the technical aspects of the present invention with reference to specific examples, which are intended to illustrate the present invention and not to limit the scope of the present invention.
The raw material liquid bovine colostrum used in examples 1 to 4 for preparing the sterile liquid bovine colostrum solution had a total solid content of 15.0%, a fat content of 4.0%, a total protein content of 4.5%, and an IgG content of 13.5 mg/mL; the microfiltration equipment adopted is a flat plate type microfilter, and the effective membrane area (single membrane) is 69.36cm
2. Steps not described in detail in the examples can be performed according to conventional operations in the art.
Example 1
The embodiment provides a sterile liquid bovine colostrum solution, which is prepared according to the following steps:
1. degreasing:
the fat in the raw material fresh bovine colostrum is completely removed by adopting a conventional degreasing process.
2. And (3) precipitating casein:
adjusting pH of bovine colostrum to 4.3 by acid precipitation, and precipitating casein.
3. And (3) filtering:
casein was roughly removed with a 140 mesh filter bag to give a filtrate. The filtrate was tested through 0.45 μm and 0.2 μm polypropylene filters under a membrane pressure differential of 2Bar, static filtration mode, and less than 20g of emulsion passed through the filter in the first 1 minute.
4. Centrifuging:
the filtrate obtained in step 3 above was centrifuged at 4000rpm for 30min to remove the precipitate and obtain a supernatant containing about 10% casein based on total protein.
5. Adjusting the pH value:
the supernatant obtained in step 4 was adjusted to pH4.5 with 8% potassium bicarbonate.
6. Preparation of sterile environment:
the microfiltration equipment and the polypropylene filter membranes with the diameters of 0.8 mu m, 0.45 mu m and 0.2 mu m are placed in a super clean bench and are irradiated for 1 hour by ultraviolet light to achieve the sterile effect.
7. Film coating:
and (3) adding the supernatant with the pH value adjusted in the step (5) into the microfiltration equipment in the step (6), enabling the supernatant to sequentially pass through polypropylene filter membranes with the diameters of 0.8 mu m, 0.45 mu m and 0.2 mu m under the pressure of about 2Bar, and then storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution for later use.
The film-passing effect of the present example is as follows:
it took about 17.3min to prepare 1000g of sterile liquid bovine colostrum solution without changing the filter.
Example 2
The embodiment provides a sterile liquid bovine colostrum solution, which is prepared according to the following steps:
1. degreasing:
and (3) completely removing fat in the bovine colostrum by adopting a conventional degreasing process.
2. And (3) precipitating casein:
adjusting pH of bovine colostrum to 4.5 by acid precipitation, and precipitating casein.
3. And (3) filtering:
casein was roughly removed using a 180 mesh filter bag to obtain a filtrate. The filtrate was tested through a 0.45 μm polypropylene filter under a membrane pressure difference of 2Bar in a static filtration mode, and less than 20g of emulsion passed through the filter in the initial 1 minute.
4. Centrifuging:
the above solution was centrifuged at 3000rpm for 20min, and the precipitate was removed to obtain a supernatant containing 20% casein based on the total protein content.
5. Adjusting the pH value:
and (3) adjusting the pH value of the supernatant obtained in the step (4) to 5.0 by using 8% potassium acetate.
6. Preparation of sterile environment:
and (3) putting the membrane passing equipment and the polypropylene filter membranes with the diameters of 0.8 mu m, 0.45 mu m and 0.2 mu m into a super clean bench, and carrying out ultraviolet irradiation for 1h to achieve the sterile effect.
7. Film coating:
and (3) adding the supernatant with the adjusted pH value in the step (5) into the membrane passing equipment in the step (6), enabling the supernatant to sequentially pass through polypropylene filter membranes with the diameters of 0.8 mu m, 0.45 mu m and 0.2 mu m under the membrane passing pressure difference of about 2Bar, and then storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
The film-passing effect of the present example is as follows:
it took about 20.1min to prepare 1000g of sterile liquid bovine colostrum solution without replacing the filter.
Example 3
The embodiment provides a sterile liquid bovine colostrum solution, which is prepared according to the following steps:
1. degreasing:
and (3) completely removing fat in the bovine colostrum by adopting a conventional degreasing process.
2. And (3) precipitating casein:
adjusting pH of bovine colostrum to 4.3 by acid precipitation, and precipitating casein.
3. And (3) filtering:
a portion of the casein was roughly removed with a 140 mesh filter bag to obtain a filtrate.
4. Centrifuging:
the filtrate was centrifuged at 4000rpm for 25min to remove the precipitate and obtain a supernatant containing approximately 13% casein based on total protein.
5. Adjusting the pH value:
the supernatant obtained in step 4 was adjusted to pH 5.5 with 8% sodium acetate.
6. Preparation of sterile environment:
and (3) putting the membrane passing equipment and the polypropylene filter membranes with the diameters of 0.8 mu m, 0.45 mu m and 0.2 mu m into a super clean bench, and carrying out ultraviolet irradiation for 1h to achieve the sterile effect.
7. Film coating:
and (3) adding the supernatant with the adjusted pH value in the step (5) into a membrane-passing device, enabling the supernatant to sequentially pass through 0.8 mu m, 0.45 mu m and 0.2 mu m filter membranes under the membrane-passing pressure difference of about 1.5Bar, and then storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
In this example, it took about 24.3min to prepare 1000g of sterile liquid bovine colostrum solution without replacing the filter membrane.
Example 4
The embodiment provides a sterile liquid bovine colostrum solution, which is prepared according to the following steps:
1. degreasing:
and (3) completely removing fat in the bovine colostrum by adopting a conventional degreasing process.
2. And (3) precipitating casein:
adjusting pH of bovine colostrum to 4.5 by acid precipitation, and precipitating casein.
3. And (3) filtering:
casein was roughly removed with a 140 mesh filter bag to give a filtrate.
4. Centrifuging:
the filtrate was centrifuged at 4000rpm for 20min to remove the precipitate and obtain a supernatant having a casein content of about 15%.
5. Acid adjustment:
and (3) adjusting the pH value of the supernatant obtained in the step (4) to 4.5 by using 5% potassium bicarbonate.
6. Preparation of sterile environment:
and (3) putting the membrane passing equipment and the polypropylene filter membranes with the diameters of 0.8 mu m, 0.45 mu m and 0.2 mu m into a super clean bench, and carrying out ultraviolet irradiation for 1h to achieve the sterile effect.
7. Film coating:
and (3) adding the supernatant with the pH value adjusted in the step (5) into a membrane-passing device, enabling the supernatant to sequentially pass through 0.8 mu m, 0.45 mu m and 0.2 mu m filter membranes under the membrane-passing pressure difference of about 2Bar, and then storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
In this example, it took about 23.8min to prepare 1000g of sterile liquid bovine colostrum solution without replacing the filter membrane.
Examination of IgG content in the products of the examples
The results of examination of the IgG content in the product after the production of the sterile liquid bovine colostrum products according to the above examples are shown in table 1 (for comparison, the values in table 1 are values obtained by converting the sterile liquid bovine colostrum of each example to the same volume as the raw liquid bovine colostrum).
TABLE 1 determination of IgG content in the different examples
| IgG content (mg/mL) | Example 1 | Example 2 | Example 3 | Example 4 |
| Theoretical value | 13.5 | 13.5 | 13.5 | 13.5 |
| Detection value | 13.03 | 12.83 | 12.93 | 12.97 |
| Amount of loss | 3.48％ | 4.96％ | 4.22％ | 3.93％ |
As can be seen from Table 1, the loss amounts of IgG in the examples of the present invention are 3.48%, 4.96%, 4.22% and 3.93%, respectively, and the loss amounts are less than 5%, and the method for preparing the sterile liquid bovine colostrum product has a good effect.
Investigation of solution effect through filter membrane under different pH conditions
Five 1000g raw materials are taken, the pH value in the step 6 is respectively adjusted to 4.5, 5.0, 5.5, 6.0 and 6.5 under the same other conditions, and the membrane passing effect of a 0.2 mu m filter membrane is respectively examined, which is shown in table 2.
TABLE 2 different pH values worth examining
| Scheme one | Scheme two | Scheme three | Scheme four | Scheme five | |
| pH value of feed liquid | 4.5 | 5.0 | 5.5 | 6.0 | 6.5 |
| By number of membranes | 1 | 1 | 1 | 4 | 6 |
| Required time of | 24.3min | 26.1min | 24.7min | 189min | 308min |
As can be seen from Table 2, under the condition of pH4.5-5.5, the time required for the same amount of bovine colostrum solution to pass through the membrane is shortest, and only 1 filter membrane is needed, while when 1000g of pH6.0 solution passes through the membrane, 4 filter membranes need to be replaced, and when 1000g of pH6.5 solution passes through the membrane, 6 filter membranes need to be replaced (in the fourth and fifth schemes, the membranes are replaced every 1 h).
Examination of the Sterilization Effect
Respectively taking 1mL of the sterile liquid bovine colostrum solution obtained in the step 7 of the embodiment 1-4, inoculating the sterile liquid bovine colostrum solution into a total bacterial colony test piece and a mould yeast test piece, putting the total bacterial colony test piece into a 37 ℃ oven for culturing for 48 hours, putting the mould yeast test piece into a 25 ℃ oven for culturing for 120 hours, and then taking out to observe whether bacteria grow or not. See table 3 for details.
TABLE 3 examination of the Sterilization Effect
As can be seen from Table 3, the liquid bovine colostrum solution in the present invention has a good bacteria-removing effect and can grow aseptically within 6 months.
Allergy study
The sterile liquid bovine colostrum solution of example 1-example 4, completely defatted and casein-removed bovine colostrum, and bovine colostrum defatted only and casein-free were used as test samples, and the test was carried out on the same healthy 72 mice in five groups of 12 mice each, each group was fed with the above bovine colostrum solution, and the feeding was carried out once every morning and night, each time 100g, for 30 days, and the health status of the mice was checked every 3 days to examine the product allergy, and the health status contents of the mice are shown in table 4.
TABLE 4 mouse health Studies
As can be seen from Table 4, when the bovine colostrum solution of the embodiments of the present invention is fed to mice, the mice have good health condition and no uncomfortable symptoms, which indicates that the bovine colostrum treated by the present process does not contain allergen and can be eaten by human body. Examples of neutral liquid dairy products to which bovine colostrum is added:
example 5 neutral liquid Dairy product with addition of bovine colostrum
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The method for preparing the liquid dairy product added with the bovine colostrum comprises the following steps:
1. preparation of pasteurized milk:
raw milk inspection: the method mainly tests sensory indexes, fat, protein, dry matters, adulteration, alcohol test and other indexes to ensure that the sensory indexes meet the national raw milk standard;
and (3) filtering: removing mechanical impurities visible to the flesh by filtration;
cooling and storing: cooling the filtered milk to 1-8 ℃, then storing the cooled milk in an original milk tank, and carrying out pasteurization within 6 hours;
pasteurization and cooling storage: and (4) sterilizing at 75-90 ℃ for 15s, cooling to 1-8 ℃, and storing in a pasteurized milk tank, wherein the storage temperature is required to be 1-8 ℃, and the storage time is not more than 6 h.
2. And (3) product ingredients:
adding 10-30 wt% of the pasteurized milk into a material melting tank, heating to 40-70 ℃, adding a stabilizer and a sweetener, melting under the stirring condition of 1400rpm for 20-30 min to obtain a material liquid 1, and keeping the temperature within the required range;
adding the rest pasteurized milk, carrying out constant volume, homogenizing, adopting a two-stage homogenizing mode, firstly adjusting the two-stage pressure to 40-50 bar, then adjusting the one-stage pressure to 150-280 bar, and storing in a mixing tank after homogenizing;
UHT sterilization: and (3) UTH sterilization is carried out on the homogenized milk in the mixing tank, the sterilization temperature is 137-142 ℃, the time is 4s, and then the milk is cooled to below 25 ℃, so as to obtain the milk base material.
The bovine colostrum is added in a sterile way: and adding the sterile bovine colostrum solution into the milk base material in an aseptic online manner to obtain the milk solution added with the bovine colostrum.
3. Sterile filling and boxing and leaving factory:
aseptically packaging the milk solution added with the bovine colostrum; spraying code and pasting a pipe; and (5) leaving the factory after being inspected to be qualified by a heat preservation test.
Example 6 neutral liquid Dairy product with addition of bovine colostrum
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The preparation method is the same as example 5.
Example 7 neutral liquid Dairy product with addition of bovine colostrum
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The preparation method is the same as example 5.
Example 8 neutral liquid Dairy product with addition of bovine colostrum
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The preparation method is the same as example 5.
Acid liquid dairy example:
example 9 acidic liquid Dairy product with addition of bovine colostrum
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The method for preparing the acidic liquid dairy product added with the bovine colostrum comprises the following steps:
1. preparation of pasteurized milk: the same as in example 5.
2. And (3) product ingredients:
adding 10-30 wt% of the pasteurized milk into a material melting tank, heating to 40-70 ℃, adding a stabilizer and a sweetener, melting under the stirring condition of 1400rpm for 20-30 min to obtain a material liquid 1, and keeping the temperature within the required range;
adding the rest pasteurized milk to a constant volume, then adding an acidity regulator, adjusting the pH value of the solution to 4.2-4.4, then homogenizing, adopting a two-stage homogenization mode, firstly adjusting the two-stage pressure to 40-50 bar, then adjusting the first-stage pressure to 150-280 bar, and storing in a mixing tank after homogenizing;
UHT sterilization: and (3) UTH sterilization is carried out on the homogenized milk in the blending tank, the sterilization temperature is 120-125 ℃, the sterilization time is 4s, and then the milk is cooled to below 25 ℃ to obtain the milk base material.
The bovine colostrum is added in a sterile way: and adding the sterile bovine colostrum solution into the milk base material in an aseptic online manner to obtain the milk added with the bovine colostrum.
3. Sterile filling and boxing and leaving factory:
aseptically packaging the milk solution added with the bovine colostrum; spraying a code and pasting a pipe; and (5) leaving the factory after being inspected to be qualified by a heat preservation test.
Example 10 acidic liquid Dairy product with bovine colostrum addition
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The preparation method is the same as example 9.
Example 11 acidic liquid Dairy product with addition of bovine colostrum
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The preparation method is the same as example 9.
Example 12 acidic liquid Dairy product with addition of bovine colostrum
Product formulation (based on 1000kg product, the selection of the raw materials meets the relevant national standard)
The preparation method is the same as example 9.
Sensory and flavor evaluation test
The products provided by the examples 5-12 of the neutral liquid milk and the acidic liquid milk, the common pure milk and the common acidic liquid milk are respectively used as test samples to carry out sensory and flavor evaluation experiments. The samples were compared for sensory and flavor every one month for six months. The results of the sensory and flavor evaluation tests are shown in tables 5 and 6.
TABLE 5 comparison of sensory and flavor results of various examples of neutral liquid dairy products at different times
TABLE 6 comparison of sensory and flavor results of acidic liquid dairy products at various times for various examples
From the experimental results in tables 5 and 6, the neutral and acidic liquid dairy products added with the bovine colostrums can be stored for more than 6 months at normal temperature, do not have any peculiar smell and color change, do not generate browning, and are beneficial to popularization and sale of the products.
Experiment of influence of stabilizer system on product stability
The stability of the samples of examples 5-12 of the neutral and acidic liquid dairy products was examined respectively, and the examination time was 6 months, and the results are shown in tables 7 and 8.
TABLE 7 investigation of product stability for various examples of neutral liquid dairy products
TABLE 8 investigation of the product stability of the examples of acidic liquid Dairy products
As can be seen from tables 7 and 8, the stabilizer of the present invention has good stability, and the product stored at room temperature for 6 months is stable and can meet the requirements.
Determination of changes in IgG content of products
The content of IgG in the samples of examples 5-12 in the neutral and acidic dairy products is respectively inspected, the inspection time is 6 months, and the results are shown in tables 9 and 10.
TABLE 9 investigation of IgG (mg/mL) content in products of examples of neutral liquid dairy products
| Time of day | Example 5 | Example 6 | Example 7 | Example 8 |
| One month | 0.561 | 1.199 | 1.964 | 2.465 |
| Two months old | 0.554 | 1.183 | 1.913 | 2.433 |
| Three months old | 0.537 | 1.172 | 1.874 | 2.409 |
| Four months old | 0.521 | 1.153 | 1.871 | 2.378 |
| Five months old | 0.507 | 1.129 | 1.792 | 2.313 |
| Six months old | 0.505 | 1.116 | 1.787 | 2.284 |
TABLE 10 examination of IgG content (mg/mL) in the products of the examples of acidic liquid dairy products
| Time of day | Example 9 | Example 10 | Example 11 | Example 12 |
| One month | 0.557 | 1.195 | 1.947 | 2.427 |
| Two months old | 0.543 | 1.178 | 1.925 | 2.403 |
| Three months old | 0.534 | 1.154 | 1.901 | 2.391 |
| Four months old | 0.521 | 1.149 | 1.883 | 2.377 |
| Five months old | 0.517 | 1.124 | 1.795 | 2.304 |
| Six months old | 0.511 | 1.109 | 1.779 | 2.269 |
As can be seen from tables 9 and 10, the loss rate of IgG in the products of examples was small.
Investigation of product sterility
The sterility test of the products provided in examples 5-12 in the neutral and acidic liquid dairy products is carried out respectively, the test period is six months, and the specific method comprises the following steps: respectively taking 1mL of samples in the examples, inoculating the samples into a total bacterial colony test piece and a mold yeast test piece under an aseptic condition, putting the total bacterial colony test piece into a 37 ℃ oven for culturing for 48 hours, putting the mold yeast test piece into a 25 ℃ oven for culturing for 120 hours, and then taking out the mold yeast test piece to observe whether bacteria grow. The results are shown in tables 11 and 12.
TABLE 11 inspection of sterility of the examples of neutral liquid dairy products
TABLE 12 sterility examination of the examples of acidic liquid Dairy products
As can be seen from tables 11 and 12, the sterility of the products in the examples of the present invention was good within six months, indicating that the products prepared by the present invention were sterile, and also indicating that the bovine colostrum prepared by the present invention was sterile and satisfied the safety requirements.
Claims (12)
1. A liquid dairy product added with bovine colostrum comprises 5-20% of sterile bovine colostrum solution in ingredients based on the total weight of the liquid dairy product, wherein the sterile bovine colostrum solution is obtained by degreasing raw material liquid bovine colostrum, removing part of casein and then carrying out microfiltration;
wherein the sterile bovine colostrum solution is prepared by the following method:
degreasing: completely removing fat in raw fresh bovine colostrum by adopting a degreasing process;
and (3) precipitating casein: adjusting pH of bovine colostrum to 4.3 by acid precipitation, and precipitating casein;
and (3) filtering: roughly removing casein by using a filter bag with 140 meshes to obtain filtrate;
centrifuging: centrifuging the obtained filtrate at 4000rpm for 30min, removing precipitate to obtain supernatant, wherein the casein content in the supernatant is 10% of the total protein content;
adjusting the pH value: regulating the obtained supernatant with 8% potassium bicarbonate to pH 4.5;
preparation of sterile environment: placing the microfiltration equipment and polypropylene filter membranes of 0.8 μm, 0.45 μm and 0.2 μm into a super clean bench, and performing ultraviolet irradiation for 1h to achieve sterile effect;
film coating: and adding the supernatant with the adjusted pH value into the sterile microfiltration equipment, sequentially passing through 0.8 mu m, 0.45 mu m and 0.2 mu m polypropylene filter membranes under the pressure of 2Bar, and storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
2. The liquid dairy product with added bovine colostrum according to claim 1, which is a neutral liquid dairy product or an acidic liquid dairy product.
3. The liquid dairy product with bovine colostrum added according to claim 2, wherein the ingredients of the neutral liquid dairy product comprise, based on the total weight of the liquid dairy product: 5-20% of sterile bovine colostrum solution, 0.8-2.5% of stabilizer, 0.05-0.25% of sweetening agent and the balance of milk;
the ingredients of the acidic liquid dairy product comprise the following components in percentage by weight of the total weight of the liquid dairy product: 5-20% of sterile bovine colostrum solution, 0.3-0.8% of stabilizer, 0.05-0.25% of sweetening agent, 0.05-0.5% of acidity regulator and the balance of milk.
4. A method of producing a liquid dairy product with added bovine colostrum according to any one of claims 1 to 3, which comprises:
mixing the liquid dairy product ingredients except the sterile bovine colostrum solution, homogenizing, sterilizing, and cooling to below 25 deg.C to obtain milk base material;
adding the sterilized bovine colostrum solution into the cooled milk base material in an online adding manner to prepare a liquid milk product added with bovine colostrum;
wherein the sterile bovine colostrum solution is prepared by the following method:
degreasing: completely removing fat in raw fresh bovine colostrum by adopting a degreasing process;
and (3) precipitating casein: adjusting pH of bovine colostrum to 4.3 by acid precipitation, and precipitating casein;
and (3) filtering: roughly removing casein by using a filter bag with 140 meshes to obtain filtrate;
centrifuging: centrifuging the obtained filtrate at 4000rpm for 30min, removing precipitate to obtain supernatant, wherein the casein content in the supernatant is 10% of the total protein content;
adjusting the pH value: regulating the obtained supernatant with 8% potassium bicarbonate to pH 4.5;
preparation of sterile environment: placing the microfiltration equipment and polypropylene filter membranes of 0.8 μm, 0.45 μm and 0.2 μm into a super clean bench, and performing ultraviolet irradiation for 1h to achieve sterile effect;
film coating: and adding the supernatant with the adjusted pH value into the sterile microfiltration equipment, sequentially passing through 0.8 mu m, 0.45 mu m and 0.2 mu m polypropylene filter membranes under the pressure of 2Bar, and storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
5. A liquid dairy product added with bovine colostrum comprises 5-20% of sterile bovine colostrum solution in ingredients based on the total weight of the liquid dairy product, wherein the sterile bovine colostrum solution is obtained by degreasing raw material liquid bovine colostrum, removing part of casein and then carrying out microfiltration;
wherein the sterile bovine colostrum solution is prepared by the following method:
degreasing: completely removing fat in the bovine colostrum by adopting a degreasing process;
and (3) precipitating casein: adjusting pH of bovine colostrum to 4.5 by acid precipitation, and precipitating casein;
and (3) filtering: roughly removing casein by using a filter bag with 180 meshes to obtain filtrate;
centrifuging: centrifuging the filtrate at 3000rpm for 20min, removing precipitate to obtain supernatant containing casein 20% of total protein;
adjusting the pH value: adjusting the pH value of the obtained supernatant to 5.0 by using 8% potassium acetate;
preparation of sterile environment: putting the membrane-passing equipment and polypropylene filter membranes of 0.8 mu m, 0.45 mu m and 0.2 mu m into a super clean bench, and carrying out ultraviolet irradiation for 1h to achieve the sterile effect;
film coating: and adding the supernatant with the adjusted pH value into the sterile membrane-passing equipment, sequentially passing through 0.8 mu m, 0.45 mu m and 0.2 mu m polypropylene filter membranes under the membrane-passing pressure difference of 2Bar, and storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
6. The liquid dairy product with added bovine colostrum according to claim 5, which is a neutral liquid dairy product or an acidic liquid dairy product.
7. The liquid dairy product with bovine colostrum added according to claim 6, wherein the ingredients of the neutral liquid dairy product comprise, based on the total weight of the liquid dairy product: 5-20% of sterile bovine colostrum solution, 0.8-2.5% of stabilizer, 0.05-0.25% of sweetening agent and the balance of milk;
the ingredients of the acidic liquid dairy product comprise the following components in percentage by weight of the total weight of the liquid dairy product: 5-20% of sterile bovine colostrum solution, 0.3-0.8% of stabilizer, 0.05-0.25% of sweetening agent, 0.05-0.5% of acidity regulator and the balance of milk.
8. A method of producing a liquid dairy product with added bovine colostrum according to any one of claims 5 to 7, which comprises:
mixing the liquid dairy product ingredients except the sterile bovine colostrum solution, homogenizing, sterilizing, and cooling to below 25 deg.C to obtain milk base material;
adding the sterilized bovine colostrum solution into the cooled milk base material in an online adding manner to prepare a liquid milk product added with bovine colostrum;
wherein the sterile bovine colostrum solution is prepared by the following method:
degreasing: completely removing fat in the bovine colostrum by adopting a degreasing process;
and (3) precipitating casein: adjusting pH of bovine colostrum to 4.5 by acid precipitation, and precipitating casein;
and (3) filtering: roughly removing casein by using a filter bag with 180 meshes to obtain filtrate;
centrifuging: centrifuging the filtrate at 3000rpm for 20min, removing precipitate to obtain supernatant containing casein 20% of total protein;
adjusting the pH value: adjusting the pH value of the obtained supernatant to 5.0 by using 8% potassium acetate;
preparation of sterile environment: putting the membrane-passing equipment and polypropylene filter membranes of 0.8 mu m, 0.45 mu m and 0.2 mu m into a super clean bench, and carrying out ultraviolet irradiation for 1h to achieve the sterile effect;
film coating: and adding the supernatant with the adjusted pH value into the sterile membrane-passing equipment, sequentially passing through 0.8 mu m, 0.45 mu m and 0.2 mu m polypropylene filter membranes under the membrane-passing pressure difference of 2Bar, and storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
9. A liquid dairy product added with bovine colostrum comprises 5-20% of sterile bovine colostrum solution in ingredients based on the total weight of the liquid dairy product, wherein the sterile bovine colostrum solution is obtained by degreasing raw material liquid bovine colostrum, removing part of casein and then carrying out microfiltration;
wherein the sterile bovine colostrum solution is prepared by the following method:
degreasing: completely removing fat in the bovine colostrum by adopting a degreasing process;
and (3) precipitating casein: adjusting pH of bovine colostrum to 4.3 by acid precipitation, and precipitating casein;
and (3) filtering: roughly removing part of casein by using a filter bag with 140 meshes to obtain filtrate;
centrifuging: centrifuging the filtrate at 4000rpm for 25min, and removing precipitate to obtain supernatant containing casein 13% of total protein;
adjusting the pH value: adjusting the obtained supernatant with 8% sodium acetate to pH 5.5;
preparation of sterile environment: putting the membrane-passing equipment and polypropylene filter membranes of 0.8 mu m, 0.45 mu m and 0.2 mu m into a super clean bench, and carrying out ultraviolet irradiation for 1h to achieve the sterile effect;
film coating: and adding the supernatant with the adjusted pH value into a membrane-passing device, sequentially passing through 0.8 mu m, 0.45 mu m and 0.2 mu m filter membranes under the membrane-passing pressure difference of 1.5Bar, and storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
10. The liquid dairy product with added bovine colostrum according to claim 9, which is a neutral liquid dairy product or an acidic liquid dairy product.
11. The liquid dairy product with added bovine colostrum according to claim 10, wherein the ingredients of the neutral liquid dairy product comprise, based on the total weight of the liquid dairy product: 5-20% of sterile bovine colostrum solution, 0.8-2.5% of stabilizer, 0.05-0.25% of sweetening agent and the balance of milk;
the ingredients of the acidic liquid dairy product comprise the following components in percentage by weight of the total weight of the liquid dairy product: 5-20% of sterile bovine colostrum solution, 0.3-0.8% of stabilizer, 0.05-0.25% of sweetening agent, 0.05-0.5% of acidity regulator and the balance of milk.
12. A method of producing a liquid dairy product supplemented with bovine colostrum according to any of claims 9-11, which comprises:
mixing the liquid dairy product ingredients except the sterile bovine colostrum solution, homogenizing, sterilizing, and cooling to below 25 deg.C to obtain milk base material;
adding the sterilized bovine colostrum solution into the cooled milk base material in an online adding manner to prepare a liquid milk product added with bovine colostrum;
wherein the sterile bovine colostrum solution is prepared by the following method:
degreasing: completely removing fat in the bovine colostrum by adopting a degreasing process;
and (3) precipitating casein: adjusting pH of bovine colostrum to 4.3 by acid precipitation, and precipitating casein;
and (3) filtering: roughly removing part of casein by using a filter bag with 140 meshes to obtain filtrate;
centrifuging: centrifuging the filtrate at 4000rpm for 25min, and removing precipitate to obtain supernatant containing casein 13% of total protein;
adjusting the pH value: adjusting the obtained supernatant with 8% sodium acetate to pH 5.5;
preparation of sterile environment: putting the membrane-passing equipment and polypropylene filter membranes of 0.8 mu m, 0.45 mu m and 0.2 mu m into a super clean bench, and carrying out ultraviolet irradiation for 1h to achieve the sterile effect;
film coating: and adding the supernatant with the adjusted pH value into a membrane-passing device, sequentially passing through 0.8 mu m, 0.45 mu m and 0.2 mu m filter membranes under the membrane-passing pressure difference of 1.5Bar, and storing the solution in a sterile sealed bag to obtain a sterile liquid bovine colostrum solution.
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