CN106198363B - Flow cytometry detection chip - Google Patents

Abstract

The invention discloses a flow cytometry detection chip, which is sequentially compounded with a first protection film, a first sealing film, a microfluid chip and a second sealing film which can be peeled from top to bottom; the first sealing film is provided with at least four electrode holes, a sample adding hole and an air outlet hole; the microfluidic chip is provided with a liquid storage pool, a sample adding pool, a detection channel, a waste liquid pool and at least two detection holes positioned at two ends of the detection channel, wherein the liquid storage pool, the sample adding pool, the detection channel and the waste liquid pool are communicated with each other from top to bottom according to the using direction of the chip; the four electrode holes are arranged at positions corresponding to the inlets of the liquid storage tank, the two detection holes and the waste liquid tank respectively; the setting position of the sample adding hole corresponds to the sample adding pool; the setting position of the air outlet corresponds to the waste liquid pool. The invention provides the power for the liquid flow by utilizing the gravity principle, so the invention does not need to be additionally driven, has small chip volume and low cost, uses miniaturized equipment and saves energy, and simultaneously sets the electrode holes for sampling detection to ensure the precision of the detection result.

Description

A flow cytometry chip

technical field

The invention relates to a flow cytometry detection chip.

Background technique

Flow particle detection is a technology that quantitatively analyzes and sorts particles in liquid one by one. It can measure particle size, shape, concentration, activity, etc. It is widely used in hematology, immunology, molecular biology and other disciplines. Applications. The Coulter's principle used in the detection means that when the particles suspended in the electrolyte pass through the small hole with the electrolyte, it replaces the same volume of electrolyte, which leads to the gap between the two electrodes inside and outside the small hole in the circuit designed with constant current. The resistance changes instantaneously to generate potential pulses, and the size and number of pulses are proportional to the size and number of particles. Sample focusing is the key technology of flow particle detection. At present, chips that focus the sample liquid by external force are used in the current detection. This type of chip integrates the detection electrode in the chip, and the volume is large. Power, and need to add buffer to the chip when using, how to add the buffer, how much to add will also affect the use of the chip.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a small and convenient flow cytometry detection chip that does not require external force to be driven.

The technical solution to achieve the purpose of the present invention is a flow cytometry chip, which is sequentially compounded with a peelable first protective film, a first sealing film, a microfluidic chip and a second sealing film from top to bottom; the first sealing film is The membrane is provided with at least four electrode holes, a sample addition hole and an air outlet hole; the microfluidic chip is provided with a connected liquid storage tank, a sample addition tank, a detection channel and a waste liquid from top to bottom according to the use direction of the chip The four electrode holes are located at the entrance of the liquid storage tank, the two detection holes and the waste liquid pool, respectively; the setting positions of the sample injection holes correspond to The sample addition pool; the setting position of the air outlet corresponds to the waste liquid pool.

Each of the electrode holes is at least one dashed line pierced through the first sealing film; the buffer solution is preset in the liquid storage tank.

Six electrode holes are provided; four detection holes are provided, two at each end of the detection channel; and one electrode hole is provided on the first sealing film at positions corresponding to the four detection holes.

On the first sealing film, a balance hole is provided between the electrode hole at the lowest position and the air outlet hole.

The air outlet hole is a non-circular hole; or both the air outlet hole and the balance hole are non-circular holes.

The depths of the liquid storage tank, the sample adding tank, the detection channel and the waste liquid tank on the microfluidic chip are different.

The first sealing film is an opaque film.

The bottom of the second sealing film is also compounded with an opaque second protective film.

The first protective film and the second sealing film are both transparent films.

The electrode hole at the highest position on the first sealing film simultaneously serves as an air inlet hole.

After adopting the above-mentioned technical scheme, the present invention has positive effects: (1) the present invention utilizes the principle of gravity to provide the power of liquid flow, so no external drive is required, the chip size is small, the cost is low, and the equipment used is miniaturized, Save energy, and set up electrode holes for sampling detection to ensure the accuracy of detection results.

(2) The electrode hole of the present invention adopts a penetrating but not hollowed-out hole, which is convenient for setting electrodes during use, and at the same time, it can block the liquid and prevent the buffer solution from spilling. Such a structure can preset the buffer solution in the chip, which is convenient detection.

(3) The balance hole of the present invention is located above the air outlet. As a supplement to the air outlet, even if bubbles are generated at the air outlet during transportation or use, the liquid can still flow smoothly.

(4) The prior art adopts circular air outlet holes, but the circular air outlet hole liquid is easy to flow inside, and the conductor liquid environment is disordered. The present invention provides non-circular balance holes and air outlet holes, and the liquid is not easy to shake, which is beneficial to Stable liquid environment.

(5) The first protective film of the present invention can form a closed environment for the chip, avoid the total dislocation of the liquid during transportation and storage, and is relatively stable relative to the liquid surface environment. Before use, there is no liquid into the waste liquid pool. Under the action of gravity, the liquid flows downward; at the same time, the first protective film can also protect the liquid in the chip from being clean.

(6) The first sealing film and the second protective film of the present invention are opaque films, which can prevent the liquid from deteriorating due to light.

(7) The second protective film of the present invention is a transparent film, which is convenient for quality inspection.

(8) The depth of each pool of the chip of the present invention is different, which is beneficial to provide different driving forces during use.

Description of drawings

In order to make the content of the present invention easier to understand clearly, the present invention will be described in further detail below according to specific embodiments and in conjunction with the accompanying drawings, wherein

FIG. 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic structural diagram of the first sealing film of the present invention.

FIG. 3 is a schematic structural diagram of the microfluidic chip of the present invention.

The numbers in the attached drawings are:

The first protective film 1, the electrode hole 21, the first electrode hole 21-1, the second electrode hole 21-2, the third electrode hole 21-3, the fourth electrode hole 21-4, the fifth electrode hole 21-5, The sixth electrode hole 21-6, the sample addition hole 22, the air outlet hole 23, the balance hole 24, the first sealing film 2, the microfluidic chip 3, the liquid storage tank 31, the sample addition tank 32, the waste liquid tank 33, the detection hole 34 , the second sealing film 4 , the second protective film 5 .

Detailed ways

(Example 1)

Referring to Figures 1, 2 and 3, a flow cytometry chip in this embodiment is sequentially compounded from top to bottom with a peelable first protective film 1, a first sealing film 2, a microfluidic chip 3, a Two sealing films 4 and a second protective film 5; the first sealing film 2 is provided with six electrode holes 21, a sample injection hole 22 and an air outlet 23; the microfluidic chip 3 is arranged from top to bottom according to the direction of use of the chip There are a connected liquid storage tank 31, a sample addition tank 32, a detection channel and a waste liquid tank 33, and two pairs of four detection holes 34 located at both ends of the detection channel; the setting positions of the six electrode holes 21 are respectively: corresponding to the liquid storage The first electrode hole 21-1 of the cell 31, the second electrode hole 21-2 corresponding to the four detection holes 34, the third electrode hole 21-3, the fifth electrode hole 21-5, the sixth electrode hole 21-6 and Corresponding to the fourth electrode hole 21-4 at the entrance of the waste liquid pool 33; of course, only four electrode holes 21 may be provided, and one at each end of the detection channel is also possible, and the accuracy of such detection data will be slightly worse. The setting position of the sample adding hole 22 corresponds to the sample adding pool 32 ; the setting position of the air outlet hole corresponds to the waste liquid pool 33 . Each electrode hole 21 is at least one dashed line pierced through the first sealing film 2 , and can be drawn into a zigzag shape as shown in FIG. 2 or other shapes; a buffer solution is preset in the liquid storage tank 31 . A balance hole 24 is provided on the first sealing film 2 between the lowest electrode hole 21 and the air outlet hole 23 . The air outlet hole 23 is a non-circular hole; or both the air outlet hole 23 and the balance hole 24 are non-circular holes. The depths of the liquid storage tank 31 , the sample addition tank 32 , the detection channel and the waste liquid tank 33 on the microfluidic chip 3 are different. The first sealing film 2 is an opaque film. The bottom of the second sealing film 4 is also compounded with an opaque second protective film 5 . Both the first protective film 1 and the second sealing film 4 are transparent films. The highest first electrode hole 21-1 on the first sealing film 2 also serves as an air inlet hole.

When in use, just tear off the first protective film 1, and insert each electrode from the electrode hole 21. Due to the scribe line, the electrode insertion is very convenient, and the detection instrument can be connected by adding the tissue liquid to be detected from the sample injection hole. test. During production, the second protective film 5 is not pasted first. Since the second sealing film 4 is transparent, the quality inspection can be carried out conveniently. After the quality inspection is passed, the second protective film 5 is pasted.

The specific embodiments described above further describe the purpose, technical solutions and beneficial effects of the present invention in further detail. It should be understood that the above descriptions are only specific embodiments of the present invention, and are not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims (6)

1. A flow cytometry chip, characterized in that: from top to bottom, a peelable first protective film (1), a first sealing film (2), a microfluidic chip (3) and a second seal are sequentially compounded Membrane (4); at least four electrode holes (21), a sample adding hole (22) and an air outlet hole (23) are arranged on the first sealing membrane (2); the microfluidic chip (3) is arranged according to the The use direction of the chip is provided with a connected liquid storage pool (31), a sample addition pool (32), a detection channel and a waste liquid pool (33) from top to bottom, and at least two detection holes (34) located at both ends of the detection channel ; The setting positions of the four electrode holes (21) correspond to the inlets of the liquid storage tank (31), the two detection holes (34) and the waste liquid pool (33) respectively; the setting of the sample adding holes (22) The position corresponds to the sample addition pool (32); the setting position of the air outlet corresponds to the waste liquid pool (33); each electrode hole (21) is at least one dashed line that penetrates the first sealing film (2); A buffer solution is preset in the liquid storage tank (31); the air outlet hole (23) is a non-circular hole; the lowest electrode hole (21) and the air outlet hole ( 23) A balance hole (24) is arranged between; the balance holes (24) are all non-circular holes; the electrode hole (21) at the highest position on the first sealing film (2) simultaneously serves as an air inlet hole. 2. A flow cytometry detection chip according to claim 1, characterized in that: six electrode holes (21) are provided; four detection holes (34) are provided, and two detection holes are provided at both ends of the detection channel. One electrode hole (21) is provided on the first sealing film (2) at positions corresponding to the four detection holes (34). 3. A flow cytometry detection chip according to claim 1 or 2, characterized in that: the liquid storage tank (31), the sample adding tank (32), the detection channel and the The depth of the waste pool (33) varies. 4 . The flow cytometry detection chip according to claim 3 , wherein the first sealing film ( 2 ) is an opaque film. 5 . 5 . The flow cytometry detection chip according to claim 4 , characterized in that: the bottom of the second sealing film ( 4 ) is further compounded with an opaque second protective film ( 5 ). 6 . 6 . The flow cytometry detection chip according to claim 5 , wherein the first protective film ( 1 ) and the second sealing film ( 4 ) are both transparent films. 7 .

Images