CN106674307A - Method for extracting stevioside from stevia rebaudiana - Google Patents
Method for extracting stevioside from stevia rebaudiana Download PDFInfo
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- CN106674307A CN106674307A CN201610947863.9A CN201610947863A CN106674307A CN 106674307 A CN106674307 A CN 106674307A CN 201610947863 A CN201610947863 A CN 201610947863A CN 106674307 A CN106674307 A CN 106674307A
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- ethanol
- stevia rebaudiana
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- 244000228451 Stevia rebaudiana Species 0.000 title claims abstract description 36
- 235000006092 Stevia rebaudiana Nutrition 0.000 title claims abstract description 36
- 235000019202 steviosides Nutrition 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 24
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 title abstract description 6
- 229940013618 stevioside Drugs 0.000 title abstract description 6
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 title abstract description 6
- 239000012535 impurity Substances 0.000 claims abstract description 36
- 239000011347 resin Substances 0.000 claims abstract description 15
- 229920005989 resin Polymers 0.000 claims abstract description 15
- 238000001179 sorption measurement Methods 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 83
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 75
- 239000004383 Steviol glycoside Substances 0.000 claims description 28
- 229930182488 steviol glycoside Natural products 0.000 claims description 28
- 235000019411 steviol glycoside Nutrition 0.000 claims description 28
- 150000008144 steviol glycosides Chemical class 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000012046 mixed solvent Substances 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 20
- 239000000706 filtrate Substances 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 13
- 239000012043 crude product Substances 0.000 claims description 10
- 239000003480 eluent Substances 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 7
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 6
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 5
- 239000000920 calcium hydroxide Substances 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 239000012065 filter cake Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000012467 final product Substances 0.000 claims description 5
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 5
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 5
- 238000011068 loading method Methods 0.000 claims description 5
- 239000002932 luster Substances 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000004064 recycling Methods 0.000 claims description 5
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000003463 adsorbent Substances 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 235000015110 jellies Nutrition 0.000 description 3
- 239000008274 jelly Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 2
- 241000208838 Asteraceae Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- CZMRCDWAGMRECN-UHFFFAOYSA-N Rohrzucker Natural products OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 241000544066 Stevia Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/20—Carbocyclic rings
- C07H15/24—Condensed ring systems having three or more rings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Seasonings (AREA)
Abstract
The invention belongs to the technical field of agricultural cultivation, and particularly relates to a method for extracting stevioside from stevia rebaudiana. The method comprises steps as follows: 1), blade treatment; 2), first impurity removal; 3), extraction; 4), second impurity removal; 5), resin adsorption; 6), collection. The method has the beneficial effects as follows: the extraction effect is good, and the purity of the obtained stevioside is high and is 95% or higher averagely.
Description
Technical field
The invention belongs to agricultural plantation technology field, and in particular to a kind of method that steviol glycoside is extracted from STEVIA REBAUDIANA.
Background technology
STEVIA REBAUDIANA (stevia rebaudiana) calls sweet chrysanthemum ﹑ sugar grass, category composite family (Compositae), Si Taiweiya
Category, original producton location is in South America subtropical zone.China started by sections such as Nanjing Botanical Garden Mem. Sun Yat-Sen, the Chinese Academy of Agricultural Sciences from 1976
Grind unit successively to plant experimentally successfully from Japan's introduction STEVIA REBAUDIANA.STEVIA REBAUDIANA contains 14 kinds of Wei amount Yuan Su 32 kinds of nutrient contents of ﹑, in vivo
Not metabolism, do not accumulate, not carcinogenic, nontoxicity, its security obtained recognizing for the tissue such as international FAO and WHO and U.S. FAD
Can, therefore it is both fabulous sugar source, is again good source of nutrition.
Stevioside, abbreviation stevioside is the class tool sweet taste extracted from dried STEVIA REBAUDIANA blade
Terpenes glycocide, it is main comprising 8 kinds of glucosides with different sugarinesses.Stevioside has high sugariness (for the 200-300 of sucrose
The characteristic of times) ﹑ (only the 1/300 of sucrose) low in calories, long-term consumption will not make one to get fat, particularly suitable obesity, diabetes,
Hypertension, artery sclerosis, carious tooth patient use, and physics, stable chemical performance, without Fermented, stevia can be extended
The shelf-life of glycosides product.Thus increasingly cause the concern and attention of people, in being widely used in food and medicine industry, praised
It is the third the natural sugar source after sucrose, beet sugar, is most rising new sugar source.
The content of the invention
It is an object of the invention to provide a kind of method that steviol glycoside is extracted from STEVIA REBAUDIANA.
The invention provides following technical scheme：
A kind of method that steviol glycoside is extracted from STEVIA REBAUDIANA, comprises the steps of,
1）Blade process, by STEVIA REBAUDIANA blade reject impurity and color and luster it is gloomy after, vacuum freeze drying is crushed, is sifted out, and obtains
STEVIA REBAUDIANA powder；
2）First time removal of impurities, to step 1) in the STEVIA REBAUDIANA powder that obtains add the n-hexane of its weight 3-5 times, stir 20-40
Minute, filtration, recycling design, take STEVIA REBAUDIANA filter cake, dry, obtain the first removal of impurities powder；
3）Extract, step 2 is added in heating and stirring device）In the first removal of impurities powder for obtaining and its weight 3-5 times ethanol
With water mixed solution, the heating-up temperature of heating and stirring device is 40-60 DEG C, and mixing speed is 200-300 rev/min, during process
Between be 20-40 minutes, then filter, take filtrate, repeat to extract 1-3 merging filtrate afterwards, filtrate is dried, obtain steviol glycoside
Crude product；
4）Second removal of impurities, by step 3）In the steviol glycoside crude product that obtains be dissolved in appropriate water, add and used after bodied ferric sulfate
Calcium hydroxide adjusts pH to 7.5, after stirring 5-15 minutes, stands 10-20 minutes, filters, obtains the second removal of impurities is filtered liquid；
5）Resin adsorption；With the HPD-100 purification with macroreticular resin steps 4 through pretreatment）In the second removal of impurities for obtaining
Filtrate, by loading after the second removal of impurities is filtered liquid reduced pressure concentration, flow velocity during HPD-100 resin adsorptions is 2 BV/h, adsorption saturation
Afterwards, first then eluted with ethyl acetate mixed solvent with ethanol with the pure water rinsing 5-15 minute that flow velocity is 4 BV/h, washed
De- speed is 3 BV/h, collects the eluent of ethanol and ethyl acetate mixed solvent, in the ethanol and ethyl acetate mixtures,
Ethanol is 5 with the volume ratio of ethyl acetate:2；
6）Collect, the ethanol collected and ethyl acetate mixed solvent eluent are concentrated by evaporation, are dried, obtain final product steviol glycoside.
Preferably, the step 3）Middle ethanol and water mixed solvent, the mass percent of ethanol is 40%-60%.
Contain some atent solvents in general polymeric adsorbent, therefore different depth must be carried out pre- according to needs are applied using before
Process：First in extraction column, resin 1-2 hours are washed with clear water adverse current, then just stream washes 1-2 hours.After putting water purification, addition is higher than
The ethanol immersion 3-4 hours of 20-30 centimetre of resin bed, then put net cleaning solution, are a cleaning process.With same method repeatedly
Outlet cleaning solution sampling is washed till in test tube plus till 3 times of amount water do not show muddiness, then with the abundant drip washing of clear water to without obvious second
Alcohol smell, that is, complete preprocessing process.
The invention has the beneficial effects as follows：Extraction effect is good, and the steviol glycoside purity for obtaining is high, average out to more than 95%.
Specific embodiment
Embodiment 1
It is a kind of from STEVIA REBAUDIANA extract steviol glycoside method, by STEVIA REBAUDIANA blade reject impurity and color and luster it is gloomy after, vacuum is cold
Jelly dry, pulverize, sift out, and obtain STEVIA REBAUDIANA powder；
Take the above-mentioned g of STEVIA REBAUDIANA powder 500 and add 1.5 Kg n-hexanes, stirring 20 minutes, filtration, recycling design take STEVIA REBAUDIANA filter
Cake, dry, obtain the first removal of impurities powder；Add in heating and stirring device the ethanol of 3 times of the first removal of impurities powder and its weight with
In water mixed solution, the ethanol and water mixed solvent, the mass percent of ethanol is 40%, the heating temperature of heating and stirring device
Spend for 40 DEG C, mixing speed is 200 revs/min, and process time is 20 minutes, is then filtered, and takes filtrate, and filtrate is dried, and is obtained
To steviol glycoside crude product；Steviol glycoside crude product is dissolved in into appropriate water, add after bodied ferric sulfate with calcium hydroxide adjust pH to
7.5, after stirring 5 minutes, 10 minutes are stood, filter, obtain the second removal of impurities is filtered liquid；With the HPD-100 macropores through pretreatment
Purification on adsorbent resins the second removal of impurities is filtered liquid, by loading after the second removal of impurities is filtered liquid reduced pressure concentration, during HPD-100 resin adsorptions
Flow velocity is 2 BV/h, after adsorption saturation, first with the pure water rinsing that flow velocity is 4 BV/h 5 minutes, then with ethanol and ethyl acetate
Mixed solvent is eluted, and elution speed is 3 BV/h, collects the eluent of ethanol and ethyl acetate mixed solvent, the ethanol
In ethyl acetate mixtures, ethanol is 5 with the volume ratio of ethyl acetate:2；By the ethanol collected and ethyl acetate mixed solvent
Eluent is concentrated by evaporation, drying, obtains final product steviol glycoside, and the purity of gained steviol glycoside is 95.8%.
Embodiment 2
It is a kind of from STEVIA REBAUDIANA extract steviol glycoside method, by STEVIA REBAUDIANA blade reject impurity and color and luster it is gloomy after, vacuum is cold
Jelly dry, pulverize, sift out, and obtain STEVIA REBAUDIANA powder；
Take the above-mentioned g of STEVIA REBAUDIANA powder 500 and add 2.0 Kg n-hexanes, stirring 30 minutes, filtration, recycling design take STEVIA REBAUDIANA filter
Cake, dry, obtain the first removal of impurities powder；Add in heating and stirring device the ethanol of 4 times of the first removal of impurities powder and its weight with
In water mixed solution, the ethanol and water mixed solvent, the mass percent of ethanol is 50%, the heating temperature of heating and stirring device
Spend for 50 DEG C, mixing speed is 250 revs/min, and process time is 30 minutes, is then filtered, and takes filtrate, and filtrate is dried, and is obtained
To steviol glycoside crude product；Steviol glycoside crude product is dissolved in into appropriate water, add after bodied ferric sulfate with calcium hydroxide adjust pH to
7.5, after stirring 10 minutes, 15 minutes are stood, filter, obtain the second removal of impurities is filtered liquid；With the HPD-100 macropores through pretreatment
Purification on adsorbent resins the second removal of impurities is filtered liquid, by loading after the second removal of impurities is filtered liquid reduced pressure concentration, during HPD-100 resin adsorptions
Flow velocity is 2 BV/h, after adsorption saturation, first with the pure water rinsing that flow velocity is 4 BV/h 10 minutes, then with ethanol and ethyl acetate
Mixed solvent is eluted, and elution speed is 3 BV/h, collects the eluent of ethanol and ethyl acetate mixed solvent, the ethanol
In ethyl acetate mixtures, ethanol is 5 with the volume ratio of ethyl acetate:2；By the ethanol collected and ethyl acetate mixed solvent
Eluent is concentrated by evaporation, drying, obtains final product steviol glycoside, and the purity of gained steviol glycoside is 96.5%.
Embodiment 3
It is a kind of from STEVIA REBAUDIANA extract steviol glycoside method, by STEVIA REBAUDIANA blade reject impurity and color and luster it is gloomy after, vacuum is cold
Jelly dry, pulverize, sift out, and obtain STEVIA REBAUDIANA powder；
Take the above-mentioned g of STEVIA REBAUDIANA powder 500 and add 2.5 Kg n-hexanes, stirring 40 minutes, filtration, recycling design take STEVIA REBAUDIANA filter
Cake, dry, obtain the first removal of impurities powder；Add in heating and stirring device the ethanol of 5 times of the first removal of impurities powder and its weight with
In water mixed solution, the ethanol and water mixed solvent, the mass percent of ethanol is 60%, the heating temperature of heating and stirring device
Spend for 60 DEG C, mixing speed is 300 revs/min, and process time is 40 minutes, is then filtered, and takes filtrate, and filtrate is dried, and is obtained
To steviol glycoside crude product；Steviol glycoside crude product is dissolved in into appropriate water, add after bodied ferric sulfate with calcium hydroxide adjust pH to
7.5, after stirring 15 minutes, 20 minutes are stood, filter, obtain the second removal of impurities is filtered liquid；With the HPD-100 macropores through pretreatment
Purification on adsorbent resins the second removal of impurities is filtered liquid, by loading after the second removal of impurities is filtered liquid reduced pressure concentration, during HPD-100 resin adsorptions
Flow velocity is 2 BV/h, after adsorption saturation, first with the pure water rinsing that flow velocity is 4 BV/h 15 minutes, then with ethanol and ethyl acetate
Mixed solvent is eluted, and elution speed is 3 BV/h, collects the eluent of ethanol and ethyl acetate mixed solvent, the ethanol
In ethyl acetate mixtures, ethanol is 5 with the volume ratio of ethyl acetate:2；By the ethanol collected and ethyl acetate mixed solvent
Eluent is concentrated by evaporation, drying, obtains final product steviol glycoside, and the purity of gained steviol glycoside is 97.2%.
The preferred embodiments of the present invention are the foregoing is only, the present invention is not limited to, although with reference to aforementioned reality
Apply example to be described in detail the present invention, for a person skilled in the art, it still can be to aforementioned each enforcement
Technical scheme described in example is modified, or carries out equivalent to which part technical characteristic.All essences in the present invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (2)
1. it is a kind of from STEVIA REBAUDIANA extract steviol glycoside method, it is characterised in that comprise the steps of,
1）Blade process, by STEVIA REBAUDIANA blade reject impurity and color and luster it is gloomy after, vacuum freeze drying is crushed, is sifted out, and obtains
STEVIA REBAUDIANA powder；
2）First time removal of impurities, to step 1) in the STEVIA REBAUDIANA powder that obtains add the n-hexane of its weight 3-5 times, stir 20-40
Minute, filtration, recycling design, take STEVIA REBAUDIANA filter cake, dry, obtain the first removal of impurities powder；
3）Extract, step 2 is added in heating and stirring device）In the first removal of impurities powder for obtaining and its weight 3-5 times ethanol
With water mixed solution, the heating-up temperature of heating and stirring device is 40-60 DEG C, and mixing speed is 200-300 rev/min, during process
Between be 20-40 minutes, then filter, take filtrate, repeat to extract 1-3 merging filtrate afterwards, filtrate is dried, obtain steviol glycoside
Crude product；
4）Second removal of impurities, by step 3）In the steviol glycoside crude product that obtains be dissolved in appropriate water, add and used after bodied ferric sulfate
Calcium hydroxide adjusts pH to 7.5, after stirring 5-15 minutes, stands 10-20 minutes, filters, obtains the second removal of impurities is filtered liquid；
5）Resin adsorption, with the HPD-100 purification with macroreticular resin steps 4 through pretreatment）In the second removal of impurities for obtaining
Filtrate, by loading after the second removal of impurities is filtered liquid reduced pressure concentration, flow velocity during HPD-100 resin adsorptions is 2 BV/h, adsorption saturation
Afterwards, first then eluted with ethyl acetate mixed solvent with ethanol with the pure water rinsing 5-15 minute that flow velocity is 4 BV/h, washed
De- speed is 3 BV/h, collects the eluent of ethanol and ethyl acetate mixed solvent, in the ethanol and ethyl acetate mixtures,
Ethanol is 5 with the volume ratio of ethyl acetate:2；
6）Collect, the ethanol collected and ethyl acetate mixed solvent eluent are concentrated by evaporation, are dried, obtain final product steviol glycoside.
2. it is according to claim 1 it is a kind of from STEVIA REBAUDIANA extract steviol glycoside method, it is characterised in that the step
3）Middle ethanol and water mixed solvent, the mass percent of ethanol is 40%-60%.
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Cited By (4)
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|---|---|---|---|---|
| CN107312548A (en) * | 2017-07-17 | 2017-11-03 | 河海大学 | A kind of organic improvement base for promoting solonchak sodium ion exchange |
| CN107722082A (en) * | 2017-09-30 | 2018-02-23 | 江苏艾斯维特生物科技有限公司 | A kind of production method of stevioside |
| WO2020063894A1 (en) * | 2018-09-30 | 2020-04-02 | 晨光生物科技集团股份有限公司 | Industrial utilization method for stevia rebaudiana and stevioside and chlorogenic acid of stevia rebaudiana |
| CN111153944A (en) * | 2019-09-26 | 2020-05-15 | 内蒙古昶辉生物科技股份有限公司 | Method for extracting stevioside by adopting simulated moving bed chromatography |
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2016
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107312548A (en) * | 2017-07-17 | 2017-11-03 | 河海大学 | A kind of organic improvement base for promoting solonchak sodium ion exchange |
| CN107312548B (en) * | 2017-07-17 | 2020-06-09 | 河海大学 | An organic modifier that promotes sodium ion exchange in saline soil |
| CN107722082A (en) * | 2017-09-30 | 2018-02-23 | 江苏艾斯维特生物科技有限公司 | A kind of production method of stevioside |
| CN107722082B (en) * | 2017-09-30 | 2020-02-04 | 江苏艾斯维特生物科技有限公司 | Production method of stevioside |
| WO2020063894A1 (en) * | 2018-09-30 | 2020-04-02 | 晨光生物科技集团股份有限公司 | Industrial utilization method for stevia rebaudiana and stevioside and chlorogenic acid of stevia rebaudiana |
| KR20210076030A (en) * | 2018-09-30 | 2021-06-23 | 첸광 바이오테크 그룹 캄파니 리미티드 | Industrial use method of Stevia rebaudiana and its stevioside and chlorogenic acid |
| KR102600268B1 (en) | 2018-09-30 | 2023-11-10 | 첸광 바이오테크 그룹 캄파니 리미티드 | Industrial use method of Stevia rebaudiana and its stevioside and chlorogenic acid |
| US11891411B2 (en) | 2018-09-30 | 2024-02-06 | Chenguang Biotech Group Co., Ltd. | Industrial utilization method for Stevia rebaudiana and stevioside and chlorogenic acid of Stevia rebaudiana |
| CN111153944A (en) * | 2019-09-26 | 2020-05-15 | 内蒙古昶辉生物科技股份有限公司 | Method for extracting stevioside by adopting simulated moving bed chromatography |
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