CN107228785B - Humidification heating device for tissue immunohistochemical section - Google Patents

Abstract

The invention belongs to the technical field of medical equipment, and discloses a humidifying and heating device for tissue immunohistochemical slicing, which is provided with a water vapor outlet hinged at the front end of a water vapor conveying pipe; the tail end of the steam conveying pipe is hinged at the top end of the humidifying and heating kettle body; a water level alarm is placed in the humidifying and heating kettle body; a temperature controller and a dry-heating prevention switch are stuck on the outer wall of the humidifying and heating kettle body; a heater is arranged at the inner bottom of the humidifying and heating kettle body; the heater is connected with a foot control switch through a wire; the outer bottom of the humidifying and heating kettle body is sleeved with a magnetic sucker or a silica gel sucker; the water level alarm, the dry heating switch and the foot control switch are all connected with the temperature controller through wires. The steam of the invention heats and humidifies the slices, keeps 100% of the slices smooth, about 90% of the slices have no wrinkles, about 80% of the slices have no cracks, reduces the waste of precious tissue specimens, ensures the structural integrity of tissue cells, and reduces the occurrence of false positives in immunohistochemical staining.

Description

Humidification and heating device for tissue immunohistochemical section

technical field

The invention belongs to the technical field of pathophysiology in medical equipment, in particular to a humidifying and heating device for tissue immunohistochemical sectioning.

Background technique

At present, after the tissue has been well processed, the paraffin-embedded tissue block should be processed before sectioning. In order to obtain a smoother section and keep the protein from being easily denatured, the paraffin-embedded box is usually first placed in a low-temperature refrigerator. However, due to the low temperature of the wax block and low indoor humidity at the beginning of slicing, each slice is prone to shrinkage, unevenness, and cracks, resulting in poor immunohistochemical results.

In summary, the problems in the prior art are:

In the existing processing of tissue immunohistochemical sections, it is impossible to ensure that each section is flat and not shrunk during the sectioning process of immunohistochemical experiments; it cannot reduce the waste of precious tissue specimens and reduce the phenomenon of false positives in immunohistochemical staining.

Contents of the invention

Aiming at the problems in the prior art, the invention provides a humidifying and heating device for tissue immunohistochemical sectioning.

The present invention is achieved in this way, a humidifying and heating device for tissue immunohistochemical section, the humidifying and heating device for tissue immunohistochemical section is provided with a water vapor outlet hinged at the front end of the water vapor delivery tube; the water vapor delivery tube The end is hinged on the top of the humidifying and heating kettle body;

A water level alarm is placed inside the humidifying and heating pot; a temperature controller and an anti-dry switch are pasted on the outer wall of the humidifying and heating pot; a heater is placed on the inner bottom of the humidifying and heating pot; The heater is connected with a foot control switch through a wire; the bottom of the body of the humidification heating pot is equipped with a magnetic suction cup or a silica gel suction cup; the water level alarm, the dry switch, and the foot control switch are all connected with a temperature controller through a wire.

Further, the water vapor conveying pipe is a freely expandable structure.

Furthermore, the openings at the front end of the water vapor outlet are various in size and round or flat in shape.

Further, the exterior of the water vapor delivery pipe is covered with an insulation layer.

Further, the foot switch is provided with multiple gears, and the multiple gears are connected to the temperature controller through wires for adjusting different temperatures.

Further, the outer bottom of the humidifying and heating pot is covered with a waterproof base.

Further, the bottom of the body of the humidifying and heating kettle is equipped with a magnetic suction cup or a silica gel suction cup;

Further, the water level line is engraved on the body of the humidifying and heating pot, and the water level alarm will alarm if the water level exceeds or falls below the set range according to the water level line of the pot body.

Further, the temperature controller is respectively connected to the water level alarm, the dry switch, and the foot switch through a single-key and multi-key combination.

Further, the water vapor outlet is hinged on the water vapor delivery pipe through a cardan shaft; the control range of the temperature controller is 30°C to 60°C; for safety and stability, a magnetic chuck is set on the outer bottom of the humidification heating pot or silicone suction cups.

Further, the humidification heating kettle body is inlaid with adjustable nuts; it can be matched with the slicer bolts, and can be installed on the side or back of the slicer; the humidification heating kettle body is a transparent glass layer or plastic layer casting structure.

Advantage of the present invention and positive effect are:

Compared with the existing slicing operation without heating and humidification treatment, the present invention heats and humidifies each slice before slicing, slices immediately after heating and humidifying, starts the patching operation and spreading operation, and keeps 100% slices flat and smooth. About 90% of the sections have no wrinkles, and about 80% of the sections have no cracks, thereby reducing the waste of precious tissue samples, ensuring the integrity of tissue cell structures, reducing false positives in immunohistochemical staining, and ensuring the reliability and accuracy of immunohistochemical results .

Description of drawings

Fig. 1 is a schematic diagram of a humidifying and heating device for tissue immunohistochemical sectioning provided in an embodiment of the present invention.

In the figure: 1. Water vapor delivery pipe; 2. Water vapor outlet; 3. Humidification and heating pot body; 4. Water level alarm; 5. Temperature controller; 6. Anti-dry switch; 7. Heater; 8. Feet Control switch; 9. Insulation layer; 10. Water level line; 11. Waterproof base; 12. Power supply; 13. Suction cup or silicone suction cup; 14. Nut.

Detailed ways

In order to further understand the content, features and effects of the present invention, the following examples are given, and detailed descriptions are given below with reference to the accompanying drawings.

The structure of the present invention will be described in detail below in conjunction with the accompanying drawings.

As shown in Figure 1, a humidifying and heating device for tissue immunohistochemical section provided by the embodiment of the present invention is provided with a water vapor outlet 2 hinged at the front end of a water vapor delivery tube 1; Heating the top of the kettle body 3;

A water level alarm 4 is placed inside the humidifying and heating kettle body 3; a temperature controller 5 and an anti-dry switch 6 are pasted on the outer wall of the humidifying and heating kettle body; A heater 7; the heater 7 is connected with a foot control switch 8 through a wire;

The water vapor delivery pipe 1 is a freely expandable structure.

The opening size of the front end of the steam outlet 2 is various.

The exterior of the water vapor delivery pipe is covered with an insulation layer 9 .

Add described foot switch 8 and be provided with multiple gears. The multiple gears are connected to the temperature controller through wires for adjusting different temperatures.

The outer bottom of the humidifying and heating kettle body 3 is covered with a waterproof base 11; the humidifying and heating kettle body is engraved with a water level line 10; the humidifying and heating kettle body is a transparent glass layer or plastic layer casting structure.

The water level alarm will alarm if the water level exceeds or falls below the set range according to the water level line of the kettle body.

The temperature controller is respectively connected to the water level alarm, the dry switch and the foot control switch through single-key and multi-key combinations.

The water vapor outlet is hinged on the water vapor delivery pipe through a cardan shaft; the control range of the temperature controller is 30°C to 60°C; for safety and stability, a magnetic suction cup or silica gel is set on the outer bottom of the humidification heating pot sucker13.

The nut 14 with adjustable position is inlaid on the humidifying and heating kettle body; it can be matched with the slicer bolts, and can be installed on the side or the back of the slicer.

The heater 7 is connected to the power supply 12 through wires.

The structure of the present invention will be further described below in conjunction with the method of use.

When the present invention is used, a suitable location is selected, a humidifying and heating kettle body is placed, a water vapor conveying pipe is installed, and the height and length of the conveying pipe are adjusted according to experimental requirements.

Turn on the main switch of the power supply, set and adjust the temperature according to the ambient temperature and other influencing factors, so that the temperature of the water vapor is suitable for slicing without affecting the quality of slicing.

When slicing, the presence and size of water vapor can be switched at any time through the foot switch.

The size and direction of the opening of the water vapor delivery pipe can be adjusted arbitrarily, and the length of the water vapor delivery pipe can be adjusted arbitrarily;

The water vapor delivery pipe and the heating and humidifying pot body can be adjusted in different directions and heights;

The water vapor delivery pipe can be equipped with an insulation layer to prevent temperature imbalance and burn the operator;

The foot control switch of the heating and humidifying pot body can adjust the intensity of heating and control the size of water vapor;

The water level alarm can control the water level, prevent dry burning, destroy the heating and humidifying pot, and affect the immunohistochemical experiment;

The pot body of the heating and humidifying pot can use visible materials, and can use scales to mark the water level, which is convenient for observation and prevents dry burning.

The present invention will be further described in conjunction with the working principle below.

When using the present invention, add bottled mineral water to the pot to ensure that it is added above the water level line, adjust the temperature control switch, set the corresponding heating temperature according to the ambient temperature and other influencing factors, and adjust the height and length of the steam delivery pipe to make the water The steam just reaches the slicer position, and the power switch is turned on, and the delivery of the steam is controlled at any time through the foot control switch, and the slice starts when the steam starts to emerge.

The water vapor of the present invention heats and humidifies the slices, keeps 100% of the slices flat, about 90% of the slices without wrinkles, and about 80% of the slices without cracks, reduces the waste of precious tissue samples, ensures the integrity of the tissue cell structure, and reduces the occurrence of immunohistochemical staining false positive.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form. Any simple modifications made to the above embodiments according to the technical essence of the present invention, equivalent changes and modifications, all belong to this invention. within the scope of the technical solution of the invention.

Claims (10)

1. A humidifying and heating device for tissue immunohistochemical sectioning, characterized in that, the humidifying and heating device for tissue immunohistochemical sectioning is provided with a water vapor outlet hinged at the front end of the water vapor delivery tube; The end is hinged on the top of the humidifying and heating pot body; A water level alarm is placed inside the humidifying and heating pot; a temperature controller and an anti-dry switch are pasted on the outer wall of the humidifying and heating pot; a heater is placed on the inner bottom of the humidifying and heating pot; The heater is connected with a foot control switch through a wire; the bottom of the body of the humidification heating pot is equipped with a magnetic suction cup or a silica gel suction cup; the water level alarm, the dry switch, and the foot control switch are all connected with a temperature controller through a wire. 2 . The humidifying and heating device for tissue immunohistochemical sectioning according to claim 1 , wherein the water vapor conveying tube is a freely retractable structure. 3 . 3. The humidifying and heating device for tissue immunohistochemical sectioning according to claim 1, characterized in that, the openings at the front end of the water vapor outlet are various in size and round or flat in shape. 4 . The humidifying and heating device for tissue immunohistochemical sectioning according to claim 1 , wherein the water vapor delivery tube is covered with a thermal insulation layer. 5 . 5. The humidifying and heating device for tissue immunohistochemical sectioning as claimed in claim 1, wherein the foot control switch is provided with multiple gears, and the multiple gears are connected to a temperature controller through wires for adjusting different temperatures . 6 . The humidifying and heating device for tissue immunohistochemical sectioning according to claim 1 , characterized in that, the outer bottom of the humidifying and heating kettle body is covered with a waterproof base. 7. The humidifying and heating device for tissue immunohistochemical sectioning according to claim 1, characterized in that, the humidifying and heating kettle body is engraved with a water level line, and the water level alarm is set according to whether the water level exceeds or falls below the water level line of the kettle body alarm range. 8. The humidifying and heating device for tissue immunohistochemical sectioning as claimed in claim 1, wherein the temperature controller is respectively connected to a water level alarm, a dry switch, and a foot control switch through a combination of a single key and a multi-key. . 9. The humidifying and heating device for tissue immunohistochemical sectioning as claimed in claim 1, wherein the water vapor outlet is hinged on the water vapor delivery pipe through a cardan shaft; the outer bottom of the humidifying and heating pot is set There are magnetic suction cups or silicone suction cups. 10. The humidifying and heating device for tissue immunohistochemical sectioning as claimed in claim 1, wherein the humidifying and heating kettle body is inlaid with adjustable position nuts; the humidifying and heating kettle body is a transparent glass layer or The plastic layer casts the structure.