CN1072565A - A kind of Nonpoisonous disinfectant - Google Patents
A kind of Nonpoisonous disinfectant Download PDFInfo
- Publication number
- CN1072565A CN1072565A CN 92114751 CN92114751A CN1072565A CN 1072565 A CN1072565 A CN 1072565A CN 92114751 CN92114751 CN 92114751 CN 92114751 A CN92114751 A CN 92114751A CN 1072565 A CN1072565 A CN 1072565A
- Authority
- CN
- China
- Prior art keywords
- wurenye
- disinfectant
- liquid
- acid
- effect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000645 desinfectant Substances 0.000 title claims abstract description 16
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims abstract description 8
- 244000005700 microbiome Species 0.000 claims abstract description 8
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 5
- WCGUUGGRBIKTOS-GPOJBZKASA-N (3beta)-3-hydroxyurs-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CC[C@@H](C)[C@H](C)[C@H]5C4=CC[C@@H]3[C@]21C WCGUUGGRBIKTOS-GPOJBZKASA-N 0.000 claims abstract description 4
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 claims abstract description 4
- JKLISIRFYWXLQG-UHFFFAOYSA-N Epioleonolsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4CCC3C21C JKLISIRFYWXLQG-UHFFFAOYSA-N 0.000 claims abstract description 4
- YBRJHZPWOMJYKQ-UHFFFAOYSA-N Oleanolic acid Natural products CC1(C)CC2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C1)C(=O)O YBRJHZPWOMJYKQ-UHFFFAOYSA-N 0.000 claims abstract description 4
- MIJYXULNPSFWEK-UHFFFAOYSA-N Oleanolinsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MIJYXULNPSFWEK-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000001530 fumaric acid Substances 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 229940100243 oleanolic acid Drugs 0.000 claims abstract description 4
- HZLWUYJLOIAQFC-UHFFFAOYSA-N prosapogenin PS-A Natural products C12CC(C)(C)CCC2(C(O)=O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OCC(O)C(O)C1O HZLWUYJLOIAQFC-UHFFFAOYSA-N 0.000 claims abstract description 4
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229940096998 ursolic acid Drugs 0.000 claims abstract description 4
- PLSAJKYPRJGMHO-UHFFFAOYSA-N ursolic acid Natural products CC1CCC2(CCC3(C)C(C=CC4C5(C)CCC(O)C(C)(C)C5CCC34C)C2C1C)C(=O)O PLSAJKYPRJGMHO-UHFFFAOYSA-N 0.000 claims abstract description 4
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 claims abstract description 3
- 229960002598 fumaric acid Drugs 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 38
- 230000000694 effects Effects 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 4
- 230000002147 killing effect Effects 0.000 claims description 4
- 241000700605 Viruses Species 0.000 claims description 3
- 241000233866 Fungi Species 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 150000007513 acids Chemical class 0.000 claims description 2
- 235000013305 food Nutrition 0.000 claims description 2
- 230000000249 desinfective effect Effects 0.000 claims 1
- 229960003742 phenol Drugs 0.000 claims 1
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 14
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 abstract description 7
- 235000010233 benzoic acid Nutrition 0.000 abstract description 4
- 239000005711 Benzoic acid Substances 0.000 abstract description 3
- 230000003115 biocidal effect Effects 0.000 abstract description 3
- 239000000284 extract Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 229960004365 benzoic acid Drugs 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 230000000840 anti-viral effect Effects 0.000 abstract 1
- 230000000680 avirulence Effects 0.000 abstract 1
- 235000008216 herbs Nutrition 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 description 13
- 210000002784 stomach Anatomy 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 231100000419 toxicity Toxicity 0.000 description 5
- 230000001988 toxicity Effects 0.000 description 5
- 241000228197 Aspergillus flavus Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 230000002070 germicidal effect Effects 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 208000005374 Poisoning Diseases 0.000 description 3
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 3
- 241000607764 Shigella dysenteriae Species 0.000 description 3
- 206010040880 Skin irritation Diseases 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000004500 asepsis Methods 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 231100000572 poisoning Toxicity 0.000 description 3
- 230000000607 poisoning effect Effects 0.000 description 3
- 229940007046 shigella dysenteriae Drugs 0.000 description 3
- 231100000475 skin irritation Toxicity 0.000 description 3
- 230000036556 skin irritation Effects 0.000 description 3
- 241001478240 Coccus Species 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- YNQLUTRBYVCPMQ-UHFFFAOYSA-N Ethylbenzene Chemical compound CCC1=CC=CC=C1 YNQLUTRBYVCPMQ-UHFFFAOYSA-N 0.000 description 2
- FLIACVVOZYBSBS-UHFFFAOYSA-N Methyl palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC FLIACVVOZYBSBS-UHFFFAOYSA-N 0.000 description 2
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 231100000321 erythema Toxicity 0.000 description 2
- 230000000527 lymphocytic effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 208000035742 Air-borne transmission Diseases 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- BDAGIHXWWSANSR-UHFFFAOYSA-N Formic acid Chemical class OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
- 240000007171 Imperata cylindrica Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000189150 Nigrospora Species 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000005557 airborne transmission Effects 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 230000003260 anti-sepsis Effects 0.000 description 1
- 230000001147 anti-toxic effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000037358 bacterial metabolism Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000001559 benzoic acids Chemical class 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000003559 chemosterilizing effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000254 damaging effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 150000002240 furans Chemical class 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000011886 postmortem examination Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Images
Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
A kind of avirulent novel disinfectant is to be main active ingredient with Wurenye.Wurenye is that extract and separate is come out from various Chinese herbs a kind ofly has solution more antibacterial, antibiotic, antiviral activity, because of including several chemical compositions that bactericidal effect is arranged such as oleanolic acid, ursolic acid, fumaric acid, succinic acid, benzoic acid in its principal component, so be a kind of avirulence, disinfectant that excitant is low, microorganism concentration is all had a bactericidal effect from 1%～100%.
Description
The present invention relates to a kind of avirulent novel Wurenye disinfectant.
Contain many microorganisms in the air, these microorganisms have very big influence to the mankind's vital movement, breathe microorganism through the people and enter in the human body with air, along with the resistance difference of everyone health, will be to everyone generation physiological change or pathological change in various degree.
According to reports; it is to influence the human life activity via various mediums that 41 kinds of important infectious diseases are arranged in the world; wherein air-borne transmission just has 14 kinds to account for the first place; Britain once carried out infection investigation the beginning of the eighties; the result shows that respiratory tract infection accounts for 30.3%; occupy first; the bacterial infection problem is also paid attention to deeply in the hospital again; in order to prevent that patient's two degree from infecting and protecting the crowd of health in the hospital; must be noted that hospital inner environment microorganism monitoring and sterilization work; countries in the world are already to air sterillization for this reason; purify and to be paid attention to, the various disinfectants of the bright development of broken hair do not have the various microorganisms of harm and then reduce the propagation of various infectious diseases and popular human health and life to reduce in the air.
Develop both at home and abroad at present, can be to the chemosterilant that the body surface air carries out disinfection not following tens kinds of a great variety, all has good Disinfection Effect in general, but these disinfectants all exist toxicity more or less, excitant and corrosivity and contaminated environment etc. are paid effect, unfavorable to health, therefore all can not use in the space that people's activity is arranged, bring very big inconvenience with regard to the industry of carrying out air sterillization for hospital, pharmaceutical factory, food industry urgent need like this, cause its range of application to be subjected to certain restriction.
The objective of the invention is to overcome the drawback of existing disinfectant existence, provide that a kind of to extract a kind of new disinfectant from natural plants be the Wurenye disinfectant, it is not only nontoxic, and have high efficiency and kill various harmful microbe characteristics, can use in the space that people's activity is arranged, be up to the present, the most novel scientific and technological invention in disinfectant.
Wurenye of the present invention is a kind of solution that separating and extracting goes out from various herbal medicine, this solution all has obvious suppression elimination effect to various bacteriums, fungi, virus, through suitably processing, can be used for sterilization, treatment disease, the mildew-resistant storage, fruit, the Vegetable Preservation that also can be used for grain, because it is natural materials, toxicity is low, antibiotic property has tangible economic benefit by force and using value widely.
The chemical analysis of 5 benevolence thimerosals, being colour analysis and the analysis of GC/MS instrument through the extract and separate gas phase, to learn that its main chemical compositions has following several:
(1) fragrant alkanes has certain antibacterial activity as toluene, ethylbenzene, cogongrass etc.
(2) polynary alkanal and ester thereof: as sad, methyl palmitate etc. strong antifungal activity is arranged, anti-cancer activity is still arranged.
(3) phenols: can make albumen precipitation, and change permeability of cell membrane, antibiotic anti-corrosive properties are strong.
(4) benzoic acids: stronger antisepsis and sterilization effect is arranged.
(5) acids: bactericidal effect (seeing accompanying drawing 1-5) is all arranged as oleanolic acid, ursolic acid, fumaric acid, succinic acid, benzoic acid etc.
(6) other: can suppress the second vinegar as furans, and disturb the metabolism of bacterium, You such as Zu class can influence bacterial metabolism and sterilization.
The Wurenye that extracts from natural plants has three kinds of A1, B1, B2 liquid, and wherein A1 liquid is white transparent aqueous solution, slightly has slight tart flavour, dissolves in the organic solvents such as ethanol, ethyl acetate, and proportion is 1.027(10 ℃) pH value is in 5～6 scopes.
Main component content is:
A, phenols are equivalent to the amount of phenol, A1 liquid must not＜0.5081mg/ml, B1 liquid must not＜0.9448mg/ml, B2 liquid must not＜2.3626mg/ml.
B, formic acid class are equivalent to benzoic amount, A1 liquid must not＜76.37mg/ml, B1 liquid must not＜26.48mg/ml, B2 liquid must not＜131.61mg/ml.
The toxicity of Wurenye and bactericidal action can be by showing in the following examples that its effect, these surveys show that embodiment just proposes the characteristic of Wurenye and to the killing effect of microorganism, and do not limit use patent application range of the present invention.
Embodiment one Wurenye acute toxic test
1, get 20 of mouselets, male and female half and half, body weight are divided into two groups at random between 17～20g, give A1 liquid for one group, and irritating stomach dosage is 0.5ml/20g; Another group is given B1 liquid, irritates stomach dosage 0.5ml/20g, observes its situation, and observes continuously a week.None dead mouse (room temperature 15 degree) as a result.
2, healthy rabbits is 4, every about 2 kilograms of body weight, 3 rabbit are given A1, B1, B2 liquid respectively, and irritating stomach dosage is 10ml/2kg, and another gives normal saline solution, irritating stomach dosage also is 10ml/2kg, observe its poisoning situation and death state, observe a week continuously, poisoning symptom and death all do not appear in 4 rabbit, above-mentioned experiment is irritated stomach dosage and is equivalent to 5000mg/kg, is per os poisoning dosage (three times of LD50＞1500mg/kg).
Embodiment two, and the oral LD50 of Wurenye measures
Get 50 of mouselets, male and female half and half are divided into five groups at random, dilute gastric infusion according to a conventional method, continue after the administration to observe a week, write down dead situation, and dead animal is in time carried out postmortem examination, record pathology situation is pressed the Bliss method and is calculated, and measurement result learns that LD50 is 1737.90mg/kg.The 95% credible scope of LD50 is 1399.6～1689.6mg/kg, the result learns thus, Wurenye toxicity is low, than phenol solution little (LD50 282mg/kg mouse stomach), also littler than benzoic acid, the administration dead mouse all betides irritated behind the stomach after 24 hours, and mouse is no abnormal when dead, be organized in pathology and dissect, there is no and find to have assorted obvious change.
Embodiment three, skin irritation test
Get the pig mouse of 24 body weight, 250～350 grams, male and female half and half, random packet and be divided into four groups of A1 liquid, B1 liquid, B2 liquid, stoste, every group 6, carry out skin husband irritant test by the DECD method, behind the coating 1 hour, 24 hours, 48 hours, 72 hours respectively, observe the situation of administration part skin husband's excitant (oedema, erythema), and with the skin check plot relatively.
Skin irritation strength assessment standard
| Intensity | Score value |
| Do not have | 0.0～0.4 |
| Slightly | 0.5～1.9 |
| Moderate | 2.0～5.9 |
| By force | 6.0～8.0 |
Skin irritation test result (room temperature, humidity 73%)
The total score value stimulus intensity of group erythema score value oedema score value
A1 liquid group 0.16 0 0.16 does not have
B1 liquid group 0.33 0 0.33 does not have
B2 liquid group 0.50 0.16 0.66 is slight
Stoste group 1.33 0.50 1.83 is slight
Thus embodiment as can be known, the excitant of Wurenye is very little, A1, B1 be nonirritant almost.
Embodiment four, and the Wurenye Disinfection Effect is measured
1, adopt the MBC method to measure the bactericidal effect of Wurenye to staphylococcus aureus, Escherichia coli, shigella dysenteriae, pseudomonas aeruginosa.
| Concentration | Bacterium liquid measure (8 * 10/ml) | Meat soup amount (ml) | Sterilization liquid measure (ml) |
| 1% | 0.5 | 1.40 | 0.02 |
| 5% | 0.5 | 1.40 | 0.10 |
| 10% | 0.5 | 1.30 | 0.20 |
| 15% | 0.5 | 1.20 | 0.30 |
| 20% | 0.5 | 1.10 | 0.40 |
Measurement result shows
(1) A1, B2 can reach bactericidal action (asepsis growth) in 10 minutes to Escherichia coli, shigella dysenteriae and pseudomonas aeruginosa effect when 15% concentration.
(2) to Escherichia coli, shigella dysenteriae and pseudomonas aeruginosa effect can reach bactericidal action (asepsis growth) in 5 minutes to B1 liquid when 15% concentration.
(3) B1 liquid can reach bactericidal action in 5 minutes in 20% concentration to golden yellow staphylococcus effect.
2, it is as follows to the sterilization effect result of Bacterium prodigiosum to adopt liquid tube method to measure Wurenye:
The average viable count sterilizing rate t P of decontaminant concentration (%)
A1 100 13.0 79.03 13.57<0.001
50 14.0 82.26 15.37<0.001
1 18.0 70.97 14.31<0.001
0 62.0 0.00
B1 100 0.7 98.87 19.04<0.001
50 11.3 81.77 19.20<0.001
1 20.0 67.74 8.09<0.05
0 62.0
50 0.3 99.48 98.62<0.001
1 37.0 35.65 6.74<0.05
0 57.5
3, it is as follows to the germicidal efficiency result of hay bacillus to adopt liquid tube method to measure Wurenye:
Average viable count sterilizing rate (%) the t P of decontaminant concentration (%)
A1 100 39.3 39.26 4.99<0.01
50 47.7 26.29 3.67<0.05
1 51.0 21.17 2.24<0.05
0 64.7 0.00
B1 100 16.3 67.4 9.01<0.001
50 31.0 38.0 3.71<0.05
1 44.0 11.4 1.52<0.05
0 50.0 0.0
50 8.70 85.74 28.60<0.001
1 64.00 0.00 0.78<0.0
4, adopting liquid tube method to measure Wurenye shows Bacillus tuberculosis's germicidal efficiency result: through repeating 2 times experimental result, at Bacillus tuberculosis's liquid is 10 to 10 o'clock, A1, the B1, the B2 liquid that add 100% concentration respectively acted on after 5 minutes, there is no this bacteria growing.
5, A1 liquid is to gonococcal germicidal efficiency in the employing liquid tube method qualitative determination Wurenye, and the result shows 100%A1 liquid effect 5 minutes, and 50% A1 liquid effect can reach good sterilization effect (asepsis growth) in 10 minutes.
Embodiment five, and Wurenye is to the bactericidal effect of mould:
Adopt liquid tube method to measure the germicidal efficiency of Wurenye to Candida albicans and Aspergillus flavus, the result is as follows:
| Disinfectant | Concentration | Average viable count is read the coccus Aspergillus flavus in vain | Sterilizing rate (%) is read the coccus Aspergillus flavus in |
| A1 | |||
| 100 | 0.0 0.0 | 100.0 100.0 | |
| 50 | 7.0 0.0 | 91.7 100.0 | |
| 25 | 7.7 7.0 | 90.2 93.0 | |
| 10 | 54.7 92.0 | 31.0 8.0 | |
| 1 | 70.4 94.0 | 6.7 6.0 | |
| 0 | 79.3 100.0 | 0.0 0.0 | |
| |
100 | 0.0 0.0 | 100.0 100.0 |
| 50 | 0.0 0.0 | 100.0 100.0 | |
| 25 | 0.6 7.0 | 99.3 93.0 | |
| 10 | 1.0 96.0 | 98.7 4.0 | |
| 1 | 48.0 102.0 | 39.5 0.0 | |
| 0 | 79.3 100.0 | 0.0 0.0 | |
| |
100 | 0.0 0.0 | 100.0 100.0 |
| 50 | 0.0 0.0 | 100.0 100.0 | |
| 25 | 0.0 0.0 | 100.0 100.0 | |
| 10 | 0.3 0.0 | 99.6 100.0 | |
| 1 | 4.7 45.0 | 94.1 100.0 | |
| 0 | 79.3 100.0 | 0.0 0.0 |
Adopt MBC to measure in the Wurenye 30%A1 liquid in addition mould, withered mould, the nigrospora of aspergillus flavus, aspergillus niger, withered mould, wood is had better bactericidal effect.
Embodiment six, and Wurenye is measured the killing effect of virus:
1, the anti-toxic sterilizing effectiveness results of the T-3 Escherichia coli being explained body with liquid tube mensuration Wurenye is as follows: in 10 minutes
The average viable count of decontaminant concentration (class) sterilizing rate (%)
A1 100.0 0.0 100.0
50.0 0.0 100.0
10.0 25.0 96.7
1.0 79.0 86.1
0.0 760.0
B1 100.0 0.0 100.0
50.0 0.0 100.0
10.0 71.0 90.0
1.0 88.0 88.4
0.0 760.0
B2 100.0 0.0 100.0
50.0 0.3 99.9
10.0 54.0 92.9
1.0 72.0 90.5
0.0 760.0
2, adopt the SPRIA method to measure the bactericidal effect of Wurenye to HBsAg:
Get 0.1mlHBsAg positive serum (RPHA method 1: 2048) and add in the 0.4ml variable concentrations Wurenye (A1, B1, B2), act on and add neutralizer effect termination in 30 minutes sterilizing test after 2 minutes, 5 minutes, 10 minutes immediately.Establish the positive (do not add Wurenye and add physiological saline) and negative (do not add the HBsAg positive serum and add physiological saline) simultaneously.Serves as effectively with P/N value＜2.1 for declaring feminine gender, and 72.1 for declaring the positive invalid standard that is.
A1 liquid is to HBsAg exercising result (P/N value)
Concentration (%) time
2 minutes 5 minutes 10 minutes
100 0.9 1.1 0.7
50 9.9 9.9 11.6
25 9.9 9.9 15.3
B1 liquid is to HBsAg exercising result (P/N value)
Concentration (%) time
2 minutes 5 minutes 10 minutes
100 1.9 1.6 2.0
50 2.6 2.6 1.8
25 16.3 9.9 9.3
B2 liquid is to HBsAg exercising result (P/N value)
Concentration (%) time
2 minutes 5 minutes 10 minutes
100 1.3 1.1 0.9
50 0.9 0.9 0.7
25 9.9 9.3 3.9
50～100 concentration, acting on 5～10 minutes has Disinfection Effect to HBsAg.
3, Wurenye reaches the lymphocytic damaging action of the mankind the bactericidal effect of being grown disease I type (HIV-1).
Prove that through experimental study A1 liquid is slight to lymphocytic toxicity in the Wurenye, to the HIV-I and killing action is arranged, valid density is between 1: 32～1: 50, and the time is 2～5 minutes.
Description of drawings:
Gas chromatogram after Fig. 1 oleanolic acid methylates
Gas chromatogram after Fig. 2 ursolic acid methylates
Gas chromatogram after Fig. 3 succinic acid methylates
Gas chromatogram after Fig. 4 fumaric acid methylates
Fig. 5 phenol mass spectrogram
Claims (4)
1, a kind of avirulent novel disinfectant is characterized in that containing effective composition Wurenye, microorganisms such as fungi, bacterium and virus are all had the effect of killing, and its concentration produces effect all at 1%～100%.
2, disinfectant as claimed in claim 1 is characterized in that the acids composition in the main component of Wurenye is that oleanolic acid, ursolic acid, fumaric acid, succinic acid, phenol etc. have disinfecting components.
3, disinfectant as claimed in claim 1 is characterized in that can be used for the molten method of liquid processes and gas and carries out disinfection.
4, disinfectant as claimed in claim 1 is characterized in that can be used for the hospital food industry, in crowd activity's environment such as pharmaceutical factory.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92114751 CN1072565A (en) | 1992-12-24 | 1992-12-24 | A kind of Nonpoisonous disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92114751 CN1072565A (en) | 1992-12-24 | 1992-12-24 | A kind of Nonpoisonous disinfectant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1072565A true CN1072565A (en) | 1993-06-02 |
Family
ID=4947078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92114751 Pending CN1072565A (en) | 1992-12-24 | 1992-12-24 | A kind of Nonpoisonous disinfectant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1072565A (en) |
-
1992
- 1992-12-24 CN CN 92114751 patent/CN1072565A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1230117A (en) | Pharmaceutical compsns. with antimicrobial activity | |
| CN106421217B (en) | A kind of solution of benzalkonium chloride and preparation method thereof | |
| CN1802165A (en) | Non-toxic mucous membrane disinfectant containing isopropyl alcohol, sesame oil, aloe vera and lemon oil | |
| CN106309412A (en) | Compound ethanol disinfectant | |
| AU2021234626A1 (en) | Compositions for disrupting biofilm formation and for treating biofilm-related disorders | |
| CN111700910A (en) | Cleaning disinfectant for preventing human from infecting animal germs | |
| CN104940191B (en) | A kind of fat-soluble nutgall catechin gallic acid palmitate disinfectant and preparation method | |
| CN112999331A (en) | Preparation method and application of biological sterilization preparation | |
| CN102363040B (en) | Antimicrobial peptide preparation for mucosal tissues | |
| CN106692171B (en) | Compound selamectin external drop for pets as well as method and application thereof | |
| CN102106347A (en) | Cinnamaldehyde, eugenol, thymol and tannin compound bactericide | |
| CN108935526A (en) | A kind of cordate houttuynia silver ion composite disinfectant | |
| CN101077362A (en) | Plant-type skin mucosa disinfecting agent | |
| CN1786134A (en) | Multifunction antiseptic sterilizing cleaning agent composition and its preparation method | |
| CN116784345B (en) | A composition for improving nitrogen absorption of wheat plants | |
| CN118161525A (en) | Copper-containing antibacterial gel and application thereof | |
| CN1895672A (en) | Multifunctional broad-spectrum obtundent of polyphyletic synergistic and its use | |
| CN1072565A (en) | A kind of Nonpoisonous disinfectant | |
| US10154996B2 (en) | Fungicidal agent | |
| CN111328811B (en) | A kind of low-concentration alcohol sterilizing and disinfecting liquid and its application | |
| CN111920911B (en) | Mosquito-proof disinfectant and preparation method thereof | |
| CN1279939C (en) | Medicinal spraying agent for treating nasitis | |
| CN1309305C (en) | Chinese herbal medicine air disinfectant | |
| RU2292919C1 (en) | Agent for skin integument disinfection | |
| JP5455540B2 (en) | How to disinfect a poultry house or barn |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |