CN107290277A - Integrated structure of optical biosensor and microfluid device - Google Patents

Abstract

The invention provides an integrated structure of an optical biosensor and a microfluid device, comprising: a bearing component for bearing and accommodating an optical biosensor; a channel layer for at least one fluid to flow; and a covering part for the inflow and outflow of at least one fluid, wherein the at least one fluid flows in from the at least one fluid inlet, flows through the optical biosensor through the at least one flow channel for sensing, and flows out from the at least one fluid outlet. The integrated structure of the optical biosensor and the microfluid device can detect the optical signal generated by the optical biosensor, transmit fluid and avoid leakage, and is suitable for integrating various forms of optical biosensors and microfluid devices.

Description

Integrated structure of optical biosensor and microfluidic device

technical field

The invention relates to an integrated structure of an optical biosensor and a microfluidic device.

Background technique

With the continuous development of electronic technology, the tentacles of its application are also constantly exploring and combining across fields. Among them, in the rapidly developing field of biology or medical science and technology, the emergence of biosensors is nothing less than a major breakthrough in modern biology-related technologies. Biosensors are generally defined as: through immobilized biomolecules (immobilized biomolecules) combined with transducers or biochips, to detect chemical substances or biomolecules inside or outside the organism or to respond specifically to them after interacting with them a device.

Biosensors meet the needs of many important measurements, especially in the determination of interactions between drugs, metabolism and other biomolecules. Although traditional analytical instruments can also achieve similar goals, the most unique feature of biosensors is the high sensitivity, high specificity or high selectivity of biosensing components and biomolecules on them, and their real-time detection. ) characteristics. The reason is that the organism itself has a variety of chemoreceptors, in other words, the organism itself is actually a collection of chemoreceptors, and these chemoreceptors have a high degree of specificity or selectivity and sensitivity . With the gradual maturity of biosensor technology, more and more biosensors of various forms are also listed on the market. Among them, biosensors combined with optical mechanisms have great application potential for the detection of proteins or nucleic acids or other biochemical molecules.

On the other hand, through the manual or mechanical operations required in traditional biochemical analysis, the microfluidic components such as micro pumps, micro valves, micro filters, micro mixers, micro pipes, micro sensors and micro reactors are intensively produced On microfluidic devices, it is actively used in biosensors for sample pretreatment, mixing, transmission, separation and detection. Its application fields can cover such as new drug development, biological and medical research, or clinical testing such as health diagnosis, disease detection, infection pathogen detection, blood screening, and even national defense military detection, forensic identification, environmental and food inspection. and other non-medical applications.

However, the existing integrated structure of biosensors and microfluidic devices is often unable to provide the quality consistency, structural integrity and high mass production of the detection module due to the limitations of applications and existing integrated processes. Therefore, how to provide an effective and flexible integrated structure of optical biosensors and microfluidic devices is an important topic in the industry at present.

Contents of the invention

An embodiment of the present invention discloses an integrated structure of an optical biosensor and a microfluidic device, including: an integrated structure of an optical biosensor and a microfluidic device, including: a bearing member provided with at least one groove for To carry and accommodate at least one optical biosensor, wherein the optical biosensor has a biosensing layer; a channel layer is arranged above the carrier and includes at least one channel for at least one fluid to flow and a cover, disposed above the channel layer, and has at least one fluid inlet and at least one fluid outlet for the inflow and outflow of at least one fluid, the at least one fluid inlet and the at least one fluid outlet It is connected with the at least one channel of the channel layer; wherein, the at least one fluid flows in from the at least one fluid inlet, and flows through the optical biosensor and the biological material on it through the at least one channel. The sensing layer is used for sensing, and then flows out from the at least one fluid outlet.

Further, the optical biosensor is fixed in the groove of the carrier by using an adhesive fixing structure, and the groove is one of a concave surface, a concave portion and a hole.

Further, the upper surface of the optical biosensor is at the same horizontal position as the upper surface of the carrier.

Further, the carrier is made of an opaque material, and a light-transmitting window is provided, and the light-transmitting window is aligned with the optical biosensor when carrying; and the light-transmitting window is made of a transparent material Become or be a hollow opening.

Further, the surface of the channel layer is modified into a hydrophilic or hydrophobic surface.

Further, the channel layer further includes at least one of at least one pump component, at least one valve component, at least one liquid mixing component, and other microfluidic components, so as to carry out the flow and pretreatment of the at least one fluid.

Further, the channel layer is a multi-layered structure with multiple layers, and its surface is modified into a hydrophilic or hydrophobic surface.

Further, the cover is made of an opaque material, and a light-transmitting window is provided, and after covering, the light-transmitting window is aligned with the optical biosensor; and the light-transmitting window is made of a transparent material Become or be a hollow opening.

Further, the flow channel layer is integrated with the cover part or the flow channel layer is integrated with the carrier part.

Another embodiment of the present invention discloses an integrated structure of an optical biosensor and a microfluidic device, including: a carrier, provided with at least one groove for carrying and accommodating at least one optical biosensor, wherein, The optical biosensor is equipped with a first biosensing layer and a second biosensing layer; an upper flow channel layer is arranged above the carrier and includes at least one first flow channel for at least one first Fluid flow; the lower flow channel layer is arranged below the carrier and includes at least one second flow channel for at least one second fluid flow; an upper cover is arranged above the upper flow channel layer and has At least one first fluid inlet and at least one first fluid outlet are used for the inflow and outflow of at least one first fluid, the at least one first fluid inlet and the at least one first fluid outlet are connected to the upper channel layer The at least one first flow channel is connected; and the lower cover is arranged below the lower flow channel layer and has at least one second fluid inlet and at least one second fluid outlet for the inflow of at least one second fluid and Outflow, the at least one second fluid inlet and the at least one second fluid outlet are connected to the at least one second flow channel of the lower flow channel layer; wherein, the at least one first fluid and the at least one A second fluid flows in from the at least one first fluid inlet and the at least one second fluid inlet of the upper cover and the lower cover respectively, and flows through the optical The optical biosensor and the first biosensing layer on it and the at least one second channel flow through the optical biosensor and the second biosensing layer on it for sensing, and then from the The at least one first fluid outlet and the at least one second fluid outlet flow out.

Further, the optical biosensor is fixed in the groove of the carrier by using an adhesive fixing structure, and the groove is one of a concave surface, a concave portion and a hole.

Further, the upper surface of the optical biosensor is at the same horizontal position as the upper surface of the carrier.

Further, the carrier is made of an opaque material, and a light-transmitting window is provided, and the light-transmitting window is aligned with the optical biosensor when carrying; and the light-transmitting window is made of a transparent material Become or be a hollow opening.

Further, the surfaces of the upper channel layer and the lower channel layer are modified into hydrophilic or hydrophobic surfaces.

Further, the upper flow channel layer and the lower flow channel layer also include at least one of at least one pump component, at least one valve component, at least one liquid mixing component, and other microfluidic components, so as to perform the at least one first Flow and pretreatment of the fluid and the at least one second fluid.

Further, the upper channel layer and the lower channel layer are a multi-layered structure with multiple layers, and their surfaces are modified to be hydrophilic or hydrophobic.

Further, the upper cover and the lower cover are made of an opaque material, and are respectively provided with a first light-transmitting window and a second light-transmitting window. After covering, the first light-transmitting window and the The second light-transmitting windows are respectively aligned with the optical biosensors; and the first light-transmitting window and the second light-transmitting window are made of a transparent material or are hollow openings.

Further, the upper runner layer is integrated with the upper cover, and the lower runner layer is integrated with the lower cover, or the upper runner layer and the lower runner layer are integrated with the carrier Integrate into one.

Description of drawings

1 is a schematic diagram of a first embodiment of an integrated structure of an optical biosensor and a microfluidic device of the present invention;

2 is a cross-sectional view of a first embodiment of an integrated structure of an optical biosensor and a microfluidic device of the present invention;

3 is a cross-sectional view of a second embodiment of an integrated structure of an optical biosensor and a microfluidic device according to the present invention.

Explanation of reference signs:

110-carrier; 111-groove; 112-optical biosensor; 113-bio-sensing layer; 120-channel layer; 130-cover; 131-fluid inlet; 132-fluid outlet; 313-first bio-sensing layer; 314-second bio-sensing layer; 320-upper channel layer; 330-lower channel layer; 340-upper cover; 350-lower cover; A-adhesive fixing structure.

detailed description

The implementation of the present invention is described below through specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific examples, and various modifications and changes can be made to the details in the description of the present invention based on different viewpoints and applications without departing from the spirit of the present invention.

It should be noted that the structures, proportions, sizes, etc. shown in the drawings attached to this specification are only used to match the content disclosed in the specification, for those who are familiar with this technology to understand and read, and are not used to limit the implementation of the present invention. Conditions, so it has no technical substantive meaning, any modification of structure, change of proportional relationship or adjustment of size, without affecting the effect and purpose of the present invention, should fall within the scope of the present invention. within the range covered by the technical content.

1 and 2 are a schematic diagram and a cross-sectional view of a first embodiment of an integrated structure of an optical biosensor and a microfluidic device according to the present invention, respectively. The integrated structure of the optical biosensor and the microfluidic device is applied to the integrated packaging of an optical biosensor and a microfluidic device. As shown in FIGS. 1 and 2 , the integrated structure of the optical biosensor and the microfluidic device includes, from bottom to top, a carrier 110 , a channel layer 120 , and a cover 130 .

The carrier 110 is provided with at least one groove 111 for carrying and accommodating at least one optical biosensor 112 , and the at least one optical biosensor 112 has a biosensing layer 113 . The channel layer 120 is disposed above the carrier 110 and includes at least one channel for at least one fluid to flow. The cover 130 is arranged above the channel layer 120 and has at least one fluid inlet 131 and at least one fluid outlet 132 for the inflow and outflow of at least one fluid. At least one channel of the layer is connected, wherein at least one fluid flows in from at least one fluid inlet 131, flows through the optical biosensor 112 through at least one channel, and the biosensing layer 113 on it for sensing, and then from At least one fluid outlet 132 flows out.

It is worth noting that, in a preferred embodiment, the optical biosensor 112 is fixed in the groove 111 of the carrier 110 using an adhesive fixing structure A; and, when the optical biosensor 112 is placed, its upper surface can be The upper surface of the carrier 110 is located at the same horizontal position. The groove 111 can be a concave surface, a recess or a hole. The carrier 110 can be made of a transparent material or an opaque material, such as polymer material, plastic, ceramic, metal, silicon wafer, glass, or other composite materials. When the carrier 110 is made of an opaque material, the carrier 110 needs to be provided with a light-transmitting window (not shown in the figure), and the light-transmitting window is aligned with the optical biosensor 112 when carrying it; and the light-transmitting window is made of a transparent The material is made or is a hollow opening.

Likewise, the channel layer 120 can be made of a transparent material or an opaque material, such as polymer material, plastic, ceramic, metal, silicon wafer, glass, or other composite materials. The surface of the channel layer 120 can be modified to be hydrophilic or hydrophobic. The flow channel layer 120 may further include at least one pump component, at least one valve component, at least one liquid mixing component, other microfluidic components or any combination thereof, for flow and pretreatment of at least one fluid. Furthermore, the flow channel layer 120 can also be a multi-layered structure with multiple layers, and its surface can be modified to be hydrophilic or hydrophobic.

The cover 130 can be made of a transparent material or an opaque material, such as polymer material, plastic, ceramic, metal, silicon wafer, glass, or other composite materials. When the cover 130 is made of an opaque material, the cover 130 needs to be provided with a light-transmitting window (not shown), and after covering, the light-transmitting window is aligned with the optical biosensor 112; and the light-transmitting window is formed by a transparent The material is made or is a hollow opening. Furthermore, the flow channel layer 120 and the cover member 130 can be integrated into an integral structure, or the flow channel layer 120 and the carrier 110 can be integrated into an integral structure to reduce subsequent packaging procedures.

It is worth noting that, since the optical biosensor 112 applicable to the present invention needs to excite the optical signal of the biosensing layer in a light-emitting manner, and receive the sensed optical signal in a light-transmissive manner, when the carrier 110 Or when the cover 130 is made of an opaque material, an additional light-transmitting window must be provided to align with the optical biosensor 112, so that the optical signal generated after the light entering and sensing can be displayed, wherein, in FIG. 1 and FIG. The first embodiment shown in 2 is made of transparent material. Moreover, in the overall structure of the present invention, in addition to the flow channel, the joints of other components, for example, the joints between the carrier 110, the bio-sensing layer 113, the flow channel layer 120, and a cover 130 can all be isolated from fluids to avoid Fluid leaks.

FIG. 3 is a cross-sectional view of a second embodiment of an integrated structure of an optical biosensor and a microfluidic device according to the present invention. The integrated structure of the optical biosensor and the microfluidic device includes: a carrier 310 , an upper channel layer 320 , a lower channel layer 330 , an upper cover 340 , and a lower cover 350 .

The carrier 310 is provided with at least one groove for carrying and accommodating at least one optical biosensor, wherein at least one optical biosensor has a first biosensing layer 313 and a second biosensing layer 314 . The upper channel layer 320 is disposed above the carrier 310 and includes at least one first channel for at least one first fluid to flow. The lower flow channel layer 330 is disposed under the carrier 310 and includes at least one second flow channel for at least one second fluid to flow. The upper cover 340 is arranged above the upper channel layer 320 and has at least one first fluid inlet and at least one first fluid outlet for the inflow and outflow of at least one first fluid, at least one first fluid inlet and at least one The first fluid outlet is connected to at least one first channel of the upper channel layer 320 . The lower cover 350 is disposed below the lower channel layer 330 and has at least one second fluid inlet and at least one second fluid outlet for the inflow and outflow of at least one second fluid, at least one second fluid inlet and at least one The second fluid outlet is connected to at least one second channel of the lower channel layer 330, wherein at least one first fluid and at least one second fluid are respectively supplied by at least one first fluid inlet of the upper cover 340 and the lower cover 350. And at least one second fluid inlet flows in, flows through at least one optical biosensor and the first biosensing layer 313 on it through at least one first flow channel, and at least one second flow channel flows through at least one optical biosensor and the second bio-sensing layer 314 thereon for sensing, and then flow out from at least one first fluid outlet and at least one second fluid outlet respectively.

It should be noted that the second embodiment is similar to the first embodiment, the main difference is that the first embodiment uses a three-layer structure, while the second embodiment uses a five-layer structure; The lower layer is provided with an upper channel layer 320, a lower channel layer 330, an upper cover 340, and a lower cover 350. Different fluids can be injected through the upper layer and the lower layer, so it is suitable for sensing applications with more complex or multi-channel reaction processes. The components in the second embodiment are similar to those in the first embodiment, and the implementation details thereof will not be repeated.

In summary, the embodiment of the present invention discloses an integrated structure of an optical biosensor and a microfluidic device. By combining the optical biosensor and the microfluidic device, the optical signal generated by the optical biosensor can be detected, and the overall The structure, except for the flow channel, can isolate fluid and avoid leakage at the joints of other components, and is suitable for integrating various forms of optical biosensors and microfluidic devices.

However, the above-mentioned embodiments are only illustrative to illustrate the effects of the present invention, and are not intended to limit the present invention. Anyone skilled in the art can modify and change the above-mentioned embodiments without departing from the spirit and scope of the present invention. . In addition, the numbers of components in the above-mentioned embodiments are only for illustrative purposes, and are not intended to limit the present invention.

Claims (18)

1. An integrated structure of an optical biosensor and a microfluidic device, characterized in that it comprises: A carrier is provided with a groove for carrying and accommodating an optical biosensor, wherein the optical biosensor is provided with a biosensing layer; A flow channel layer is disposed above the carrier and includes at least one flow channel for at least one fluid to flow; and A cover is arranged above the channel layer and has at least one fluid inlet and at least one fluid outlet for the inflow and outflow of at least one fluid, the at least one fluid inlet and the at least one fluid outlet are connected to the at least one fluid outlet The at least one channel of the channel layer is connected; Wherein, the at least one fluid flows in from the at least one fluid inlet, flows through the optical biosensor and the biosensing layer on it through the at least one channel for sensing, and then flows from the at least one fluid inlet Fluid exits out. 2. The integrated structure of an optical biosensor and a microfluidic device as claimed in claim 1, wherein the optical biosensor is fixed in the groove of the carrier using an adhesive fixing structure, so that The groove is one of a concave surface, a concave portion and a hole. 3 . The integrated structure of an optical biosensor and a microfluidic device according to claim 1 , wherein the upper surface of the optical biosensor is at the same level as the upper surface of the carrier. 4 . 4. The integrated structure of an optical biosensor and a microfluidic device as claimed in claim 1, wherein the carrier is made of an opaque material, and a light-transmitting window is set, and the transparent window is set when carrying the carrier. The light window is aligned with the optical biosensor; and the light-transmitting window is made of a transparent material or is a hollow opening. 5 . The integrated structure of an optical biosensor and a microfluidic device according to claim 1 , wherein the surface of the channel layer is modified into a hydrophilic or hydrophobic surface. 6. The integrated structure of an optical biosensor and a microfluidic device as claimed in claim 1, wherein the channel layer further comprises at least one pump component, at least one valve component, at least one liquid mixing component, other microfluidic components, etc. At least one of the fluid components is used for the flow and pretreatment of the at least one fluid. 7. The integrated structure of optical biosensor and microfluidic device as claimed in claim 1, wherein the channel layer is a multi-layered structure with multiple layers, and its surface is modified to be hydrophilic or Hydrophobic surface. 8. The integrated structure of optical biosensor and microfluidic device as claimed in claim 1, characterized in that, the cover is made of an opaque material, and a light-transmitting window is set, and after covering, the transparent The light window is aligned with the optical biosensor; and the light-transmitting window is made of a transparent material or is a hollow opening. 9. The integrated structure of an optical biosensor and a microfluidic device according to claim 1, wherein the channel layer is integrated with the cover or the channel layer is integrated with the carrier as one. 10. An integrated structure of an optical biosensor and a microfluidic device, characterized in that it comprises: A carrier is provided with a groove for carrying and accommodating an optical biosensor, wherein the optical biosensor has a first biosensing layer and a second biosensing layer; An upper flow channel layer is arranged above the carrier and includes at least one first flow channel for at least one first fluid to flow; The lower flow channel layer is arranged below the carrier and includes at least one second flow channel for at least one second fluid to flow; An upper cover is arranged above the upper channel layer and has at least one first fluid inlet and at least one first fluid outlet for the inflow and outflow of at least one first fluid, and the at least one first fluid inlet and the at least one first fluid outlet is connected to the at least one first channel of the upper channel layer; and The lower cover is arranged below the lower channel layer and has at least one second fluid inlet and at least one second fluid outlet for the inflow and outflow of at least one second fluid, the at least one second fluid inlet and The at least one second fluid outlet is connected to the at least one second channel of the lower channel layer; Wherein, the at least one first fluid and the at least one second fluid flow in from the at least one first fluid inlet and the at least one second fluid inlet of the upper cover and the lower cover respectively, The at least one first flow channel flows through the optical biosensor and the first biosensing layer thereon, and the at least one second flow channel flows through the optical biosensor and the second biosensor layer thereon. The bio-sensing layer is used for sensing, and flows out from the at least one first fluid outlet and the at least one second fluid outlet respectively. 11. The integrated structure of an optical biosensor and a microfluidic device as claimed in claim 10, wherein the optical biosensor is fixed in the groove of the carrier using an adhesive fixing structure, so that The groove is one of a concave surface, a concave portion and a hole. 12 . The integrated structure of an optical biosensor and a microfluidic device according to claim 10 , wherein the upper surface of the optical biosensor is at the same level as the upper surface of the carrier. 13 . 13. The integrated structure of an optical biosensor and a microfluidic device as claimed in claim 10, wherein the carrier is made of an opaque material, and a light-transmissive window is set, and the transparent The light window is aligned with the optical biosensor; and the light-transmitting window is made of a transparent material or is a hollow opening. 14 . The integrated structure of an optical biosensor and a microfluidic device according to claim 10 , wherein the surfaces of the upper channel layer and the lower channel layer are modified into hydrophilic or hydrophobic surfaces. 15. The integrated structure of an optical biosensor and a microfluidic device as claimed in claim 10, wherein the upper channel layer and the lower channel layer further comprise at least one pump component, at least one valve component, at least At least one of a liquid mixing component and other microfluidic components is used to carry out the flow and pretreatment of the at least one first fluid and the at least one second fluid. 16. The integrated structure of optical biosensor and microfluidic device as claimed in claim 10, characterized in that, the upper flow channel layer and the lower flow channel layer are a multi-layered structure with multiple layers, and its surface is modified The texture becomes a hydrophilic or hydrophobic surface. 17. The integrated structure of an optical biosensor and a microfluidic device as claimed in claim 10, wherein the upper cover and the lower cover are made of an opaque material, and a first light-transmitting window is respectively provided and a second light-transmitting window, after covering, the first light-transmitting window and the second light-transmitting window are respectively aligned with the optical biosensor; and the first light-transmitting window and the second light-transmitting window The light-transmitting window is made of a transparent material or is a hollow opening. 18. The integrated structure of optical biosensor and microfluidic device as claimed in claim 10, characterized in that, the upper channel layer is integrated with the upper cover, and the lower channel layer is integrated with the lower The cover part is integrated or the upper flow channel layer and the lower flow channel layer are integrated with the bearing part.