CN107474104A - A kind of multistage Protein Separation collecting device of improved structure - Google Patents
A kind of multistage Protein Separation collecting device of improved structure Download PDFInfo
- Publication number
- CN107474104A CN107474104A CN201710859421.3A CN201710859421A CN107474104A CN 107474104 A CN107474104 A CN 107474104A CN 201710859421 A CN201710859421 A CN 201710859421A CN 107474104 A CN107474104 A CN 107474104A
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- Prior art keywords
- housing
- filter
- side wall
- pipe
- rotary drum
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of multistage Protein Separation collecting device of improved structure, including housing, the inside of housing is provided with dividing plate, housing includes upper chamber and lower chambers, and the bottom chamber of the housing is provided with filter, chromatographic column and first pump housing, and one end of filter is connected with feed pipe, the side wall of feed pipe through housing extends to the outside of housing, feed pipe is provided with the first control valve and flowmeter, and the first control valve and flowmeter are located at the outside of housing, and the inside of filter is provided with filter membrane.The present invention passes through the combination to multiple separation equipments, realize the requirement of multi-stage separation albumen, solve the separation and purifying for mixing complicated albumen, the separation of continuous high-efficient is formed, improves separative efficiency, while S-shaped circulating line and heater are provided with the side wall of housing, increase the separation solubility of protein, it is arranged on simultaneously by multiple separators in a housing, it is compact-sized, occupy little space.
Description
Technical field
The present invention relates to the multistage albumen point in pharmaceutical proteins purification technique field, more particularly to a kind of improved structure
From collecting device.
Background technology
Protein Separation typically individually uses chromatographic column, usually according to each component in sample mixture in stationary phase and mobile phase
Middle distribution coefficient is different, and so as to a kind of method of separation, existing separation method generally use fills micelle chromatographic column, passes through parent
Hydrophobic relation etc. carries out Separation of Proteins, still, when protein component is complicated, when finely being separated, it is difficult to realize effectively,
The separation of high-purity, in the chromatographic column subsequent processes with protein isolate of formation, the albumen of heterogeneity is easily again
Mixing, be this it is proposed that a kind of multistage Protein Separation collecting device of improved structure, to solve the above problems.
The content of the invention
The invention aims to solve shortcoming present in prior art, and a kind of multistage of the improved structure proposed
Protein Separation collecting device.
To achieve these goals, present invention employs following technical scheme：
A kind of multistage Protein Separation collecting device of improved structure, including housing, the inside of housing are provided with dividing plate, and housing includes upper
Chamber and lower chambers, the bottom chamber of the housing are provided with filter, chromatographic column and first pump housing, and one end of filter connects
Feed pipe is connected to, the side wall of feed pipe through housing extends to the outside of housing, and feed pipe is provided with the first control valve and flow
Meter, the first control valve and flowmeter are located at the outside of housing, and the inside of filter is provided with filter membrane, and filter is away from feed pipe
One end be connected with chromatographic column, the one end of chromatographic column away from filter is provided with first pump housing, and the outlet end of first pump housing passes through
First conduit is connected with centrifugal device, and centrifugal device includes motor and rotary drum, and the top of housing is provided with motor, the output shaft of motor
It is connected with rotary drum and rotary drum is located in the upper chamber of housing, the bottom of rotary drum is provided with rotating disc, and rotating disc is arranged on dividing plate, the
One conduit is connected to the bottom of rotary drum through dividing plate and rotating disc, is provided with multiple through holes in the side wall of rotary drum, in the side wall of housing
Provided with waste liquid collecting pipe, one end of waste liquid collecting pipe extends to the outside of housing, and the side wall of housing is hollow structure, side wall
Interior to be provided with S-shaped circulating line, the both ends of S-shaped circulating line extend respectively to the outside of housing, the top connection of S-shaped circulating line
There is water inlet pipe, water inlet pipe is provided with heater, and the bottom of S-shaped circulating line is connected with outlet pipe, and the top of the housing is provided with
Collecting box, collecting box are located at the side of motor, are connected between collecting box and housing by the second conduit, and the second conduit is provided with the
Two pump housings.
Preferably, the other end of the water inlet pipe and outlet pipe is respectively connected with water storage box, and water inlet pipe is provided with the second electricity
Magnet valve.
Preferably, the waste liquid collecting pipe is located at upper chamber's side wall bottom of housing.
Preferably, the sectional area of the rotary drum is taper.
Preferably, the top of the collecting box is provided with refrigerating plant, and insulation is equipped with the side wall of collecting box and housing
Layer, and housing is provided with thermometer.
The present invention proposes a kind of multistage Protein Separation collecting device of improved structure：Pass through the group to multiple separation equipments
Close, realize the requirement of multi-stage separation albumen, solve the separation and purifying for mixing complicated albumen, form point of continuous high-efficient
From, separative efficiency is improved, while S-shaped circulating line and heater are provided with the side wall of housing, increase the separation of protein
Solubility, while be arranged on by multiple separators in a housing, it is compact-sized, occupy little space.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the multistage Protein Separation collecting device of improved structure proposed by the present invention；
Fig. 2 is a kind of structural representation of the filter of the multistage Protein Separation collecting device of improved structure proposed by the present invention
Figure；
Fig. 3 is that a kind of partial structurtes of the filter of the multistage Protein Separation collecting device of improved structure proposed by the present invention are shown
It is intended to.
In figure：1 housing, 2 feed pipes, 3 first control valves, 4 flowmeters, 5 waste liquid collecting pipes, 6 S-shaped circulating lines, 7
Heater, 8 second solenoid valves, 9 motors, 10 second pump housings, 11 collecting boxs, 12 rotary drums, 13 outlet pipes, 14 rotating discs, 15 first
The pump housing, 16 chromatographic columns, 17 filter membranes, 18 filters.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.
Reference picture 1-3, a kind of multistage Protein Separation collecting device of improved structure, including housing 1, the inside of housing 1 are set
There is dividing plate, housing 1 includes upper chamber and lower chambers, and the bottom chamber of housing 1 is provided with filter 18, the pump of chromatographic column 16 and first
Body 15, one end of filter 18 are connected with feed pipe 2, and the side wall of feed pipe 2 through housing 1 extends to the outside of housing 1, entered
Expects pipe 2 is provided with the first control valve 3 and flowmeter 4, and the first control valve 3 and flowmeter 4 are located at the outside of housing 1, filter
18 inside is provided with filter membrane 17, and the one end of filter 18 away from feed pipe 2 is connected with chromatographic column 16, and chromatographic column 16 is away from filtering
One end of device 18 is provided with first pump housing 15, and the outlet end of first pump housing 15 is connected with centrifugal device by the first conduit, centrifugation
Device includes motor 9 and rotary drum 12, and the top of housing 1 is provided with motor 9, and the output shaft of motor 9 is connected with rotary drum 12 and rotary drum 12
In the upper chamber of housing 1, the bottom of rotary drum 12 is provided with rotating disc 14, and rotating disc 14 is arranged on dividing plate, and the first conduit is worn
Cross dividing plate and rotating disc 14 is connected to the bottom of rotary drum 12, multiple through holes are provided with the side wall of rotary drum 12, are set in the side wall of housing 1
There is a waste liquid collecting pipe 5, one end of waste liquid collecting pipe 5 extends to the outside of housing 1, and the side wall of housing 1 is hollow structure, side
S-shaped circulating line 6 is provided with wall, the both ends of S-shaped circulating line 6 extend respectively to the outside of housing 1, the top of S-shaped circulating line 6
End is connected with water inlet pipe, and water inlet pipe is provided with heater 7, and the bottom of S-shaped circulating line 6 is connected with outlet pipe 13, the top of housing 1
End is provided with collecting box 11, and collecting box 11 is located at the side of motor 9, is connected between collecting box 11 and housing 1 by the second conduit, the
Two conduits are provided with second pump housing 10, and the other end of water inlet pipe and outlet pipe 13 is respectively connected with water storage box, and water inlet pipe is provided with
Second solenoid valve 8, waste liquid collecting pipe 5 are located at upper chamber's side wall bottom of housing 1, and the sectional area of rotary drum 12 is taper, collect
The top of case 11 is provided with refrigerating plant, and heat-insulation layer is equipped with the side wall of collecting box 11 and housing 1, and housing 1 is provided with temperature
Degree meter.
Embodiment：Starting first pump housing 15 and heater 7, heater 7 heats to the liquid in S-shaped circulating line 6,
Liquid in housing 1 is heated, mixed protein solution is entered filter 18 by first pump housing 15 by feed pipe 2,
Multiple filter membranes 17 on filter 18 filter to protein solution, are filtered again by chromatographic column 16 after filtering is good, mistake
Entered after filter is good by first pump housing 15 in rotary drum 12, motor 9 drives rotary drum 12 to rotate, and mixed protein solution is centrifuged
Filtering, after centrifugation is good, egg mix white liquor forms bubble and entered by second pump housing 10 in collecting box 11, is carried out by refrigerating plant
Cooling condensation, egg mix white liquor waste liquid is discharged by waste liquid collecting pipe 5, while can be respectively mounted in the bottom corner of the present apparatus
There is universal wheel, universal wheel is provided with locking device.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (5)
1. a kind of multistage Protein Separation collecting device of improved structure, including housing（1）, housing（1）Inside be provided with dividing plate, shell
Body（1）Including upper chamber and lower chambers, it is characterised in that the housing（1）Bottom chamber be provided with filter（18）, chromatography
Post（16）With first pump housing（15）, filter（18）One end be connected with feed pipe（2）, feed pipe（2）Through housing（1）'s
Side wall extends to housing（1）Outside, feed pipe（2）It is provided with the first control valve（3）And flowmeter（4）, the first control valve（3）
And flowmeter（4）Positioned at housing（1）Outside, filter（18）Inside be provided with filter membrane（17）, filter（18）It is remote
Feed pipe（2）One end be connected with chromatographic column（16）, chromatographic column（16）Away from filter（18）One end be provided with first pump housing
（15）, first pump housing（15）Outlet end centrifugal device is connected with by the first conduit, centrifugal device includes motor（9）And rotary drum
（12）, housing（1）Top be provided with motor（9）, motor（9）Output shaft be connected with rotary drum（12）And rotary drum（12）Positioned at housing
（1）Upper chamber in, rotary drum（12）Bottom be provided with rotating disc（14）, rotating disc（14）On dividing plate, the first conduit is worn
Cross dividing plate and rotating disc（14）It is connected to rotary drum（12）Bottom, rotary drum（12）Side wall on be provided with multiple through holes, housing（1）'s
Side wall is provided with waste liquid collecting pipe（5）, waste liquid collecting pipe（5）One end extend to housing（1）Outside, housing（1）'s
Side wall is hollow structure, and S-shaped circulating line is provided with side wall（6）, S-shaped circulating line（6）Both ends extend respectively to housing（1）
Outside, S-shaped circulating line（6）Top be connected with water inlet pipe, water inlet pipe is provided with heater（7）, S-shaped circulating line（6）
Bottom be connected with outlet pipe（13）, the housing（1）Top be provided with collecting box（11）, collecting box（11）Positioned at motor（9）
Side, collecting box（11）With housing（1）Between connected by the second conduit, the second conduit is provided with second pump housing（10）.
A kind of 2. multistage Protein Separation collecting device of improved structure according to claim 1, it is characterised in that it is described enter
Water pipe and outlet pipe（13）The other end be respectively connected with water storage box, and water inlet pipe is provided with second solenoid valve（8）.
3. the multistage Protein Separation collecting device of a kind of improved structure according to claim 1, it is characterised in that described useless
Abandon liquid collecting pipe（5）Positioned at housing（1）Upper chamber's side wall bottom.
4. the multistage Protein Separation collecting device of a kind of improved structure according to claim 1, it is characterised in that described turn
Drum（12）Sectional area be taper.
A kind of 5. multistage Protein Separation collecting device of improved structure according to claim 1, it is characterised in that the receipts
Header（11）Top be provided with refrigerating plant, and collecting box（11）And housing（1）Side wall in be equipped with heat-insulation layer, and housing
（1）It is provided with thermometer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710859421.3A CN107474104A (en) | 2017-09-21 | 2017-09-21 | A kind of multistage Protein Separation collecting device of improved structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710859421.3A CN107474104A (en) | 2017-09-21 | 2017-09-21 | A kind of multistage Protein Separation collecting device of improved structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107474104A true CN107474104A (en) | 2017-12-15 |
Family
ID=60585628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710859421.3A Pending CN107474104A (en) | 2017-09-21 | 2017-09-21 | A kind of multistage Protein Separation collecting device of improved structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107474104A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6139746A (en) * | 1999-02-22 | 2000-10-31 | Kopf; Henry B. | Method and apparatus for purification of biological substances |
| US20030104571A1 (en) * | 2001-12-05 | 2003-06-05 | Smith Mark L. | Flexible method and apparatus for high throughput production and purification of multiple proteins |
| US20120149885A1 (en) * | 2011-04-10 | 2012-06-14 | Therapeutic Proteins International Llc | Downstream bioprocessing device |
| CN205420248U (en) * | 2016-03-18 | 2016-08-03 | 绿琪（北京）生物科技有限公司 | Multistage albumen separation collecting device |
| CN106591117A (en) * | 2016-12-30 | 2017-04-26 | 天津市诺奥科技发展有限公司 | Purifying and separating device for enzyme |
| CN206173353U (en) * | 2016-09-19 | 2017-05-17 | 天津鑫正牧农蛋白质有限公司 | Improve device of quickly separating phosphoric acid albumen of structure |
| CN206315290U (en) * | 2016-08-24 | 2017-07-11 | 谢凤妮 | A kind of Chinese medicine extract isolates and purifies system |
| CN206345853U (en) * | 2016-12-30 | 2017-07-21 | 天津市诺奥科技发展有限公司 | High alkaline proteases purify process units |
-
2017
- 2017-09-21 CN CN201710859421.3A patent/CN107474104A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6139746A (en) * | 1999-02-22 | 2000-10-31 | Kopf; Henry B. | Method and apparatus for purification of biological substances |
| US20030104571A1 (en) * | 2001-12-05 | 2003-06-05 | Smith Mark L. | Flexible method and apparatus for high throughput production and purification of multiple proteins |
| US20120149885A1 (en) * | 2011-04-10 | 2012-06-14 | Therapeutic Proteins International Llc | Downstream bioprocessing device |
| CN205420248U (en) * | 2016-03-18 | 2016-08-03 | 绿琪（北京）生物科技有限公司 | Multistage albumen separation collecting device |
| CN206315290U (en) * | 2016-08-24 | 2017-07-11 | 谢凤妮 | A kind of Chinese medicine extract isolates and purifies system |
| CN206173353U (en) * | 2016-09-19 | 2017-05-17 | 天津鑫正牧农蛋白质有限公司 | Improve device of quickly separating phosphoric acid albumen of structure |
| CN106591117A (en) * | 2016-12-30 | 2017-04-26 | 天津市诺奥科技发展有限公司 | Purifying and separating device for enzyme |
| CN206345853U (en) * | 2016-12-30 | 2017-07-21 | 天津市诺奥科技发展有限公司 | High alkaline proteases purify process units |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171215 |
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| RJ01 | Rejection of invention patent application after publication |