CN107723237B - Multipurpose culture dish for auxiliary reproduction test - Google Patents

Abstract

The invention discloses a multi-purpose culture dish for assisted reproduction testing; it includes a culture dish and a dish cover. The culture dish includes a dish main body, an egg placement cup, a plurality of sperm placement cups and a plurality of sperm channels; a columnar holder is provided in the middle of the main body of the dish. platform, the egg placement cup is fixed on the cylindrical support; multiple sperm placement cups surround the egg placement cups and are located in the main body of the dish; the height of the mouth of the multiple sperm placement cups is slightly lower than the height of the bottom of the egg placement cup , multiple sperm placement cups have sperm outlet openings on their outer walls near the mouth of the cup, and multiple sperm outlet openings on the outer wall of the egg placement cup near the bottom of the cup; multiple sperm channels correspond to the sperm outlet openings and the opposite positions. The sperm is introduced into the mouth. Beneficial effects: multiple sperm channels are arranged at an angle, so that the survival of the fittest in sperm swimming can be better reflected, so that only the most active sperm can swim through the sperm channels to the egg placement cup and combine with high-quality eggs. Furthermore, the present invention It has strong practicality and good use effect.

Description

Multi-purpose petri dish for assisted reproduction experiments

Technical field

The present invention relates to the technical field of medical equipment, and specifically, to a multi-purpose culture dish for assisted reproduction testing.

Background technique

A petri dish, as the name suggests, is a laboratory vessel used for microorganism or cell culture. A traditional petri dish consists of a disc-shaped dish body and a cover, which is generally made of plastic or glass. Plastic is suitable for laboratory inoculation, streaking and bacterial isolation operations, and can also be used for the cultivation of plant materials. Glass can be used for plant materials, microbial culture and adherent culture of animal cells.

For traditional in vitro fertilization culture dishes, sperm placement chambers and egg placement chambers are generally set directly in the corresponding culture dishes, and a sperm guide channel is horizontally connected in the middle of the outer wall between them to ensure good activity. The sperm can run to the egg placement cavity through the corresponding sperm guide channel and combine with the egg placed in it, so as to achieve the purpose of in vitro egg fertilization. Although this method is widely used, it still has certain flaws and shortcomings.

Specifically, on the one hand, sperm with good activity cannot find the sperm guide channel and remain disoriented in the corresponding sperm placement cavity, while sperm with poor activity may run to the egg placement cavity through the corresponding sperm guide channel. The combination with the egg placed in it cannot effectively guarantee the combination of excellent sperm and egg, which will affect the quality of the offspring produced and is more likely to reduce the development rate of fertilized embryos and the success rate of embryo transplantation. In addition, On the one hand, most active sperm are suspended in the upper inner part of the corresponding sperm placement cavity, most of the high-quality eggs sink to the bottom of the corresponding egg placement cavity, and most of the eggs with poor quality are suspended in the upper inner part of the corresponding egg placement cavity. Because of the corresponding The exit of the sperm guide channel is located in the middle of the corresponding egg placement cavity. In this case, there are also sperms with good activity or low activity that combine with eggs of poor quality, so it is still not possible to efficiently ensure that the sperm with good activity and high-quality eggs are combined. Moreover, the existing in vitro fertilization culture dish has a single function and cannot be used as a culture dish for assisted reproduction experiments, making it not very practical.

Therefore, the existing in vitro fertilization culture dishes need to be improved.

Contents of the invention

The object of the present invention is to overcome the above-mentioned shortcomings of the prior art and provide a multi-purpose culture dish for assisted reproduction testing.

The technical solution adopted by the present invention to solve the existing technical problems is: a multi-purpose petri dish for assisted reproduction testing, which includes a petri dish and a lid used to cover the petri dish. The petri dish includes a dish body, an egg Placement cup, multiple sperm placement cups and multiple sperm channels;

Wherein, a cylindrical support is fixed in the inner middle of the main body of the dish, and the egg placement cup is fixed on the cylindrical support; a plurality of sperm placement cups surround the egg placement cup and are evenly fixed. It is arranged in the main body of the dish, and the height of the mouth of the plurality of sperm placing cups is slightly lower than the height of the cup bottom of the egg placing cup, and the outer wall of the plurality of sperm placing cups is close to the cup. Each mouth is provided with a sperm introduction port facing the egg placement cup, and the outer wall of the egg placement cup is provided with a plurality of sperm introduction ports facing the sperm placement cup at the corresponding position near the bottom of the cup; The sperm channel is correspondingly fixed between the sperm lead-out port and the sperm lead-in port at opposite positions; an egg filter is fixed at each sperm lead-in port.

The above technical solutions are further elaborated below:

Preferably, the mesh size of the egg filter is smaller than the diameter of the egg and larger than the diameter of the sperm.

Preferably, the height of the opening of the egg placement cup is lower than the height of the opening of the upper end of the main body of the dish.

Preferably, a fixing groove is provided downward on the upper end of the cylindrical support; and the outer wall of the lower end of the egg placement cup is fixed in the fixing groove.

Preferably, the top walls of the plurality of sperm channels are respectively provided with a plurality of ventilation holes evenly along their length directions.

Preferably, the main body of the dish is fixed with a protective ring formed integrally with it and surrounding its outer wall;

An overflow groove is formed between the inner wall of the protective ring and the outer wall of the dish body, and the height of the upper edge of the protective ring is higher than the height of the upper end of the dish body.

Preferably, the outer wall of the protective ring is provided with a label groove for fitting the indication label.

Preferably, the outer wall of the protective ring is provided with multiple circles of anti-slip texture.

Preferably, a T-shaped switch valve is fixed upward on the top wall of the plurality of sperm channels near the sperm introduction inlets at corresponding positions.

The beneficial effects of the present invention are:

First, the present invention provides a multi-purpose petri dish for assisted reproduction experiments. When implemented, on the one hand, the egg placement cup is fixed in the inner middle of the dish body, and a plurality of sperm placement cups are provided. The placing cups are evenly distributed around the egg placing cups, so that the sperms in multiple sperm placing cups can effectively compete to combine with the eggs in the corresponding egg placing cups, so as to ensure the survival of the fittest. On the one hand, multiple sperm channels are arranged at an angle, so that the height of the sperm outlet is lower than the height of the sperm inlet, which better reflects the survival of the fittest in sperm swimming, so that only the sperm with the best activity can pass through the corresponding sperm channel. Upstream to the corresponding egg placement cup and combined with the egg, and based on common sense, most active sperm are suspended in the upper part of the corresponding sperm placement cup, and most high-quality eggs sink to the bottom of the corresponding egg placement cup. The sperm introduction port is provided on the outer wall of the sperm placement cup and close to the mouth of the cup. The sperm introduction port is provided on the outer wall of the egg placement cup and close to the bottom of the cup. In this way, the sperm passage can Prioritize the active sperm to be guided upstream along the inner cavity to the egg placement cup, so as to ultimately ensure that the sperm with the best activity and the fastest speed are combined with the high-quality eggs in the egg placement cup, thereby enabling Comprehensively ensuring the quality of the reproduced offspring can greatly improve the development rate of fertilized embryos and the success rate of embryo transplantation. Furthermore, the present invention has strong practicability and good use effects.

Second, in this technical solution, on the one hand, the mesh diameter of the egg filter is smaller than the diameter of the egg and larger than the diameter of the sperm. In this way, it can ensure the passage of sperm and block the passage of eggs, so as to avoid the loss of eggs. On the other hand, In one aspect, the height of the opening of the egg placing cup is lower than the height of the opening of the upper end of the main body of the dish, so as to avoid overflow of the culture fluid contained therein, and the top walls of the plurality of sperm channels are along its A plurality of ventilation holes are provided evenly in the length direction, so that the culture solution in the sperm channel is less likely to generate bubbles and hinder the swimming of sperm. Furthermore, the present invention has high reliability in use.

Third, in this embodiment, on the one hand, a protective ring integrally formed with the main body of the dish and surrounding the outer wall is fixed on the outside of the main body of the dish, and a protective ring is formed between the inner wall of the protective ring and the outer wall of the main body of the dish. It is an overflow tank, and the height of the upper edge of the protective ring is higher than the height of the opening of the upper end of the main body of the dish. In this way, even if the culture medium in the main body of the dish overflows, it will only It will overflow to the overflow tank and will not flow out to the workbench and cause pollution. On the other hand, the top walls of the plurality of sperm channels close to the sperm introduction inlets at corresponding positions are each fixed with a T-shape upward. The switch valve, when a plurality of the T-shaped switch valves are manually closed, will close a plurality of the sperm channels, so that the egg placement cup and the plurality of sperm placement cups are isolated from each other, so that Other culture fluids for assisted reproduction experiments can be placed in the egg placement cup and multiple sperm placement cups, so that the present invention can be used as a culture dish for assisted reproduction experiments, making it multi-purpose and comprehensive in function, and further, The use effect of the present invention can reach the best.

Description of the drawings

Figure 1 is a schematic diagram of the overall structure of a multi-purpose petri dish for assisted reproduction testing according to the present invention;

Figure 2 is an enlarged view of the sperm placement cup and the egg placement cup connected through the sperm channel in Figure 1;

The realization of the purpose, functional features and advantages of the present invention will be further described with reference to the embodiments and the accompanying drawings.

Reference number:

Petri dish 10;

Dish main body 101; cylindrical support 1011; egg placement cup 102; sperm placement cup 103; sperm channel 104; egg filter 1041; ventilation hole 1042; protective ring 105; label groove 1051; anti-slip texture 1052; overflow groove 106 ; T-shaped switching valve 107.

Detailed ways

The technical solution of the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments in order to understand the essence of the present invention more clearly and intuitively.

As shown in Figure 1 and Figure 2;

The invention provides a multi-purpose petri dish for assisted reproduction testing, including a petri dish 10 and a lid used to cover the petri dish 10 .

The improvement of the present invention is that: the culture dish 10 includes a dish body 101, an egg placement cup 102, a plurality of sperm placement cups 103 and a plurality of sperm channels 104;

Among them, a cylindrical support 1011 is fixed in the inner middle of the dish body 101, and the egg placement cup 102 is fixed on the cylindrical support 1011; a plurality of sperm placement cups 103 surround the egg placement. The cup 102 is uniformly fixed in the main body 101 of the dish, and the height of the openings of the sperm placing cups 103 is slightly lower than the height of the bottom of the egg placing cup 102. The outer wall of the sperm placement cup 103 is provided with a sperm outlet toward the egg placement cup 102 near its cup opening. The outer wall of the egg placement cup 102 is provided with a pair of corresponding holes toward the corresponding position near the bottom of the cup. The sperm introduction port of the sperm placement cup 103; a plurality of the sperm channels 104 are correspondingly fixed between the sperm introduction ports and the sperm introduction ports at opposite positions; each sperm introduction port is fixed respectively There is an egg filter 1041.

Based on the above, it is clear that the multi-purpose petri dish 10 for assisted reproduction experiments provided by the present invention, during specific implementation:

On the one hand, the egg placement cup 102 is fixed in the inner middle of the dish body 101, and a plurality of the sperm placement cups 103 are evenly distributed around the egg placement cup 102, so that multiple sperm placement cups 103 can be effectively held. The sperm in the sperm placement cup 103 compete fairly to combine with the eggs in the corresponding egg placement cup 102, so that the survival of the fittest can be ensured.

On the other hand, the height of the cup openings of the plurality of sperm placing cups 103 is slightly lower than the height of the cup bottom of the egg placing cup 102. The outer walls of the plurality of sperm placing cups 103 are close to the cup openings respectively. A sperm introduction port is provided toward the egg placement cup 102. The outer wall of the egg placement cup 102 is provided with a plurality of sperm introduction ports corresponding to the sperm placement cups 103 at corresponding positions near the bottom of the cup; Each of the sperm channels 104 is correspondingly fixed between the sperm lead-out port and the sperm lead-in port at opposite positions; from this, it can be deduced that the height of the multiple sperm lead-out ports is lower than the height of the sperm lead-in port, so that the height of the sperm lead-out ports 104 is lower than the height of the sperm lead-in port. Each of the sperm channels 104 is arranged at an angle, which better reflects the survival of the fittest as sperm swim upward, so that only the sperm with the best activity can travel upstream through the corresponding sperm channel 104 to the corresponding egg placement cup 102 to combine with the egg.

Moreover, combined with common sense, most active sperm are suspended in the upper part of the corresponding sperm placement cup 103, and most high-quality eggs sink to the bottom of the corresponding egg placement cup 102, and the sperm outlet is provided in the sperm placement cup 102. The outer wall of the cup 103 is close to the mouth of the cup, and the sperm introduction port is provided on the outer wall of the egg placement cup 102 and close to the bottom of the cup. In this way, the sperm channel 104 can preferentially guide the sperm with the best activity along the edge. Its inner cavity reaches upstream to the egg placement cup 102, which ultimately ensures that the sperm with the best activity and the fastest swimming are combined with the high-quality eggs in the egg placement cup 102, thereby fully ensuring reproduction. The quality of offspring can greatly improve the development rate of fertilized embryos and the success rate of embryo transplantation.

Furthermore, the present invention has strong practicability and good use effect.

It should be noted that in actual implementation, the diameter of the sperm is much smaller than the diameter of the egg. Therefore, in this technical solution, the mesh diameter of the egg filter 1041 is set to be smaller than the diameter of the egg and larger than the diameter of the sperm.

In this way, it can ensure the passage of sperm and block the passage of eggs, thus preventing egg loss.

It should be clarified that the diameter of a human egg is approximately 0.1 mm to 0.2 mm, and a mature human sperm is approximately 60 microns long, making the diameter of a mature human sperm less than 60 microns (0.06 mm).

Therefore, preferably, in this technical solution, the mesh size of the egg filter 1041 is set to 0.06-0.08 mm.

Preferably, in this technical solution, the mesh size of the egg filter 1041 is set to 0.07 mm.

Moreover, in this embodiment, the height of the opening of the egg placement cup 102 is lower than the height of the opening of the upper end of the main body 101 of the cup.

In this way, it is possible to prevent the culture medium contained therein from overflowing.

And during specific implementation, the upper end of the cylindrical support 1011 is provided with a fixing groove (not shown) downward; the outer wall of the lower end of the egg placement cup 102 is fixed in the fixing groove (not shown).

Preferably, in this technical solution, a plurality of ventilation holes 1042 are opened evenly along the length direction of the top walls of the plurality of sperm channels 104.

In this way, the culture fluid in the sperm channel 104 is less likely to generate bubbles and hinder the swimming of sperm.

Furthermore, the present invention has high reliability in use.

Further, during specific implementation, the dish body 101 is fixed with a protective ring 105 formed integrally with it and surrounding its outer wall;

Wherein, the lower edge of the inner wall of the protective ring 105 is connected to the lower edge of the outer wall of the dish body 101 to form an integral body, so that an overflow groove is formed between the inner wall of the protective ring 105 and the outer wall of the dish body 101 106. The height of the upper edge of the protective ring 105 is higher than the height of the upper opening of the dish body 101 .

In this way, even if the culture medium in the dish body 101 overflows too much, it will only overflow to the overflow tank 106 and will not flow out to the workbench and cause pollution.

Furthermore, during specific implementation, the outer wall of the protective ring 105 is provided with a label groove 1051 for fitting the indication label.

In this way, the label slot 1051 is provided to facilitate the labeling to indicate the purpose of the present invention and the culture medium placed therein, making it clear and easy to identify.

Furthermore, during specific implementation, the outer wall of the protective ring 105 is provided with multiple anti-skid textures 1052 around it.

In this way, when holding the outer wall of the protective ring 105 of the present invention, the hand and the anti-slip texture 1052 offset, so that the hand can effectively prevent slipping and falling off.

Furthermore, during specific implementation, a T-shaped switch valve 107 is fixed upward on the top wall of the plurality of sperm channels 104 close to the sperm introduction inlet at the corresponding position.

In this way, when the plurality of T-shaped switching valves 107 are manually closed, the plurality of sperm channels 104 are closed, so that the egg placement cup 102 and the plurality of sperm placement cups 103 interact with each other. Partition allows other culture fluids for assisted reproduction experiments to be placed in the egg placement cup 102 and multiple sperm placement cups 103, so that the present invention can be used as the culture dish 10 for assisted reproduction experiments, making it versatile. ,Full-featured.

Furthermore, the use effect of the present invention can be optimal.

It should be added that during specific implementation, the number of sperm placement cups 103 is preferably set to four, and the four sperm placement cups 103 are evenly distributed on the front, back, left, and right of the egg placement cup 102. The outer wall of the protective ring 105 is also provided with two gripping ears (not shown) opposite to the outside in order to facilitate the hand holding.

Other embodiments, etc., will not be described one by one here.

In summary, the overall structure of the present invention is simple, easy to implement, easy to operate, strong in practicability, strong in specificity, and low in manufacturing cost. In the improvement of the structure and the improvement of technology, there is no need to increase too much cost, making the present invention more cost-effective. The invention must have good market promotion value. The invention will be very popular and can be effectively popularized.

The above descriptions are only preferred embodiments of the present invention, and do not limit the scope of patent protection of the present invention. Any transformation of equivalent structures or equivalent processes using the contents of the description and drawings of the present invention, or direct or indirect application in other Relevant technical fields are similarly included in the scope of patent protection of the present invention.

Claims (7)

1. The utility model provides an auxiliary reproduction test is with multipurpose culture dish, includes the culture dish and is used for the lid to close the dish lid of culture dish, its characterized in that: the culture dish comprises a dish main body, an ovum placing cup, a plurality of sperm placing cups and a plurality of sperm channels;

wherein, a columnar saddle is fixedly arranged in the middle part of the dish main body, and the ovum placing cup is fixedly arranged on the columnar saddle; the plurality of sperm placing cups are uniformly fixed in the dish main body around the ovum placing cup, the heights of the cup mouths of the sperm placing cups are slightly lower than the cup bottom height of the ovum placing cup, the outer walls of the sperm placing cups are respectively provided with a sperm outlet which faces the ovum placing cup near the cup mouths, and the outer walls of the ovum placing cups are provided with a plurality of sperm inlets which face the sperm placing cup at corresponding positions near the cup bottom; the sperm channels are correspondingly and fixedly arranged between the sperm outlet and the sperm inlet which are opposite in position; an ovum filter screen is fixedly arranged at each sperm guide inlet;

the upper end surface of the columnar support is provided with a fixing groove downwards; the outer wall of the lower end of the ovum placing cup is clamped and fixed in the fixing groove;

the top walls of the sperm channels are respectively and uniformly provided with a plurality of vent holes along the length direction.

2. The multipurpose petri dish for assisted reproduction test according to claim 1, wherein: the mesh aperture of the ovum filter screen is smaller than the diameter of the ovum and larger than the diameter of the sperm.

3. The multipurpose petri dish for assisted reproduction test according to claim 1, wherein: the cup mouth of the ovum placing cup is lower than the open top of the dish main body.

4. The multipurpose petri dish for assisted reproduction test according to claim 1, wherein: the outer side of the dish main body is fixedly provided with a protection ring which is integrally formed with the dish main body and surrounds the outer wall of the dish main body;

the inner wall of the protection ring and the outer wall of the dish main body form an overflow groove, and the height of the upper edge of the protection ring is higher than the height of the upper end opening of the dish main body.

5. The multipurpose petri dish for assisted reproduction test according to claim 4, wherein: the outer wall of the protection ring is provided with a label groove for attaching the indication label.

6. The multipurpose petri dish for assisted reproduction test according to claim 4, wherein: the outer wall of the protection ring is surrounded by a plurality of circles of anti-skid lines.

7. The multipurpose petri dish for assisted reproduction test according to any one of claims 1 to 6, wherein: the top walls of the sperm channels are close to the sperm inlets at the corresponding positions, and T-shaped switch valves are respectively and fixedly arranged upwards.