CN107744404A - Subpatellar fat pad fragment of tissue collection system and collection method in Arthroscopic Operative - Google Patents
Subpatellar fat pad fragment of tissue collection system and collection method in Arthroscopic Operative Download PDFInfo
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- 210000000577 adipose tissue Anatomy 0.000 title claims abstract description 29
- 239000012634 fragment Substances 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 13
- 210000001519 tissue Anatomy 0.000 title claims abstract description 12
- 238000009530 blood pressure measurement Methods 0.000 claims abstract description 5
- 239000002184 metal Substances 0.000 claims description 4
- 210000004417 patella Anatomy 0.000 claims description 2
- 230000008676 import Effects 0.000 claims 3
- 238000011010 flushing procedure Methods 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 2
- 210000000130 stem cell Anatomy 0.000 abstract 1
- 238000001356 surgical procedure Methods 0.000 description 6
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 210000000629 knee joint Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000000426 patellar ligament Anatomy 0.000 description 2
- 238000005498 polishing Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000000281 joint capsule Anatomy 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods
- A61B17/56—Surgical instruments or methods for treatment of bones or joints; Devices specially adapted therefor
- A61B17/58—Surgical instruments or methods for treatment of bones or joints; Devices specially adapted therefor for osteosynthesis, e.g. bone plates, screws or setting implements
- A61B17/88—Osteosynthesis instruments; Methods or means for implanting or extracting internal or external fixation devices
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Abstract
Description
技术领域technical field
本发明属于医疗器械技术领域，具体涉及关节镜术中髌下脂肪垫组织碎片收集系统及收集方法。The invention belongs to the technical field of medical devices, and in particular relates to a collection system and a collection method of infrapatellar fat pad tissue fragments during arthroscopy.
背景技术Background technique
髌下脂肪垫位于髌骨下方、髌韧带后面，是关节囊内略呈三角形的脂肪组织。它充填于膝关节前部，有减少髌韧带与骨关节面摩擦和为周围无血供组织提供营养代谢的作用。但由于膝关节特殊的生理结构，关节镜术中为了保障手术视野清晰，时常对髌下脂肪垫进行适量的切除，刨削后的脂肪组织随生理盐水一并抛弃。但近期的研究表明，髌下脂肪组织中含有充足的间充质干细胞，其具有的多样分化能力，特别是软骨样分化能力为治疗膝关节软骨缺损提供了可能。The infrapatellar fat pad is located below the patella and behind the patellar ligament, and is a slightly triangular-shaped fatty tissue in the joint capsule. It fills the front of the knee joint, reduces the friction between the patellar ligament and the articular surface of the bone, and provides nutrition and metabolism for the surrounding non-vascular tissues. However, due to the special physiological structure of the knee joint, in order to ensure a clear surgical field of view during arthroscopic surgery, an appropriate amount of infrapatellar fat pad is often removed, and the planed fat tissue is discarded together with normal saline. However, recent studies have shown that infrapatellar adipose tissue contains sufficient mesenchymal stem cells, and its diverse differentiation capabilities, especially chondroid differentiation capabilities, provide the possibility for the treatment of knee cartilage defects.
发明内容Contents of the invention
有鉴于此，本发明专利的目的在于提供一种关节镜术中髌下脂肪垫组织碎片收集系统及收集方法，通过该收集系统及收集方法对关节镜术中刨削后的髌下脂肪垫组织进行收集，为进一步提取间充质干细胞提供原料。In view of this, the purpose of the patent of the present invention is to provide a collection system and collection method of infrapatellar fat pad tissue fragments in arthroscopic surgery, through which the infrapatellar fat pad tissue after planing in arthroscopic surgery is collected Collected to provide raw materials for further extraction of mesenchymal stem cells.
本发明的目的是通过以下技术方案实现的：The purpose of the present invention is achieved through the following technical solutions:
关节镜术中髌下脂肪垫组织碎片收集系统，包括关节刨刀，还包括收集容器和负压装置，所述收集容器包括设置有内部负压腔的外壳和设置在所述内部负压腔中的滤筒，所述外壳的一端封闭，另一端设置有与所述内部负压腔连通的出口，所述出口与所述负压装置连通，所述滤筒的进口穿过所述外壳的封闭端与所述关节刨刀的负压出口连通；所述收集系统上还设置有旁通管路和测压管路，所述旁通管路包括用于连通所述关节刨刀的负压出口与所述负压装置之间的旁通管，所述旁通管上设置有用于开闭所述旁通管路的开关阀Ⅰ，所述旁通管路还包括设置在所述滤筒进口的开关阀Ⅱ和设置在所述外壳出口的开关阀Ⅲ；所述测压管路包括用于连通所述开关阀Ⅱ的出口与所述开关阀Ⅲ的进口的连通管，所述连通管上设置有用于检测所述滤筒内外压差的压差传感器及与所述压差传感器连通的报警器。The system for collecting tissue fragments of infrapatellar fat pad in arthroscopic operation includes a joint planer, and also includes a collection container and a negative pressure device, and the collection container includes a shell provided with an internal negative pressure chamber and a A filter cartridge, one end of the housing is closed, and the other end is provided with an outlet communicating with the internal negative pressure chamber, the outlet communicates with the negative pressure device, and the inlet of the filter cartridge passes through the closed end of the housing It communicates with the negative pressure outlet of the joint planer; the collection system is also provided with a bypass pipeline and a pressure measurement pipeline, and the bypass pipeline includes a negative pressure outlet for communicating with the joint planer and the The bypass pipe between the negative pressure devices, the bypass pipe is provided with a switch valve I for opening and closing the bypass pipeline, and the bypass pipeline also includes a switch valve arranged at the inlet of the filter cartridge II and the on-off valve III arranged at the outlet of the shell; the pressure measuring pipeline includes a connecting pipe for communicating the outlet of the on-off valve II with the inlet of the on-off valve III, and the connecting pipe is provided with a A differential pressure sensor for detecting the differential pressure inside and outside the filter cartridge and an alarm communicated with the differential pressure sensor.
进一步，所述外壳和滤筒之间还设置有端盖。Further, an end cover is also arranged between the shell and the filter cartridge.
进一步，所述端盖包括盖体和一体设置的具有与所述滤筒和所述关节刨刀的负压出口连通的内部通道的连接体，所述连接体的一端设置有与所述滤筒相连的螺纹，另一端设置有与所述关节刨刀出口管相连的接口Ⅰ，所述盖体上设置有与所述外壳相连的螺纹。Further, the end cover includes a cover body and a connecting body integrally provided with an internal channel communicating with the negative pressure outlet of the filter cartridge and the joint planer, and one end of the connecting body is provided with a The other end is provided with an interface I connected with the outlet pipe of the joint planer, and the cover is provided with a thread connected with the outer shell.
进一步，所述滤筒包括滤筒架和滤网，所述滤网为150目金属滤网。Further, the filter cartridge includes a filter cartridge holder and a filter screen, and the filter screen is a 150-mesh metal filter screen.
进一步，所述外壳和所述端盖间还设置有密封垫。Further, a gasket is also provided between the shell and the end cover.
进一步，所述负压装置为空气泵。Further, the negative pressure device is an air pump.
本发明还提供一种关节镜术中髌下脂肪垫组织碎片收集系统的收集方法，主要包括以下步骤：The present invention also provides a collection method of the infrapatellar fat pad tissue fragment collection system during arthroscopic surgery, which mainly includes the following steps:
(1)通过打开开关阀Ⅱ和开关阀Ⅲ、关闭开关阀Ⅰ，使收集容器处于工作状态，旁通管路处于断开状态；(1) By opening on-off valve II and on-off valve III and closing on-off valve I, the collection container is in working condition and the bypass pipeline is in disconnected state;
(2)使用关节刨刀对髌下脂肪垫进行抛削，并启动空气泵，通过空气泵形成的负压将髌下脂肪垫碎片吸入收集容器中，同时将冲洗液排出；(2) Use a knuckle planer to polish the infrapatellar fat pad, and start the air pump, and the negative pressure formed by the air pump will suck the infrapatellar fat pad fragments into the collection container, and discharge the washing liquid at the same time;
(3)当报警器发出报警声时，提示收集容器的阻力增大，不利于收集，应更换收集容器，停止抛削和空气泵，并关闭开关阀Ⅱ和开关阀Ⅲ，更换收集容器；(3) When the alarm sounds, it indicates that the resistance of the collection container increases, which is not conducive to collection. The collection container should be replaced, the throwing and air pump should be stopped, and the on-off valve II and on-off valve III should be closed to replace the collection container;
(4)再次启动空气泵，并打开开关阀Ⅱ和开关阀Ⅲ，继续抛削、收集；(4) Start the air pump again, and open the on-off valve II and on-off valve III to continue throwing and collecting;
(5)抛削结束，关闭开关阀Ⅱ和开关阀Ⅲ，打开开关阀Ⅰ，使收集容器处于旁通状态，同时，旁通管路处于工作状态，便于后续手术的进行；(5) After the polishing is completed, close the on-off valve II and on-off valve III, open the on-off valve I, so that the collection container is in a bypass state, and at the same time, the bypass pipeline is in a working state, which is convenient for subsequent operations;
(6)取下收集容器，收集结束。(6) Take off the collection container, and the collection ends.
本发明的有益效果在于：采用该收集系统对关节镜术中刨削后的髌下脂肪垫组织进行收集，为进一步提取间充质干细胞提供原料。The beneficial effect of the present invention is that: the collection system is used to collect the infrapatellar fat pad tissue after planing in arthroscopic operation, so as to provide raw materials for further extracting mesenchymal stem cells.
附图说明Description of drawings
为了使本发明的目的、技术方案和有益效果更加清楚，本发明提供如下附图进行说明：In order to make the purpose, technical scheme and beneficial effect of the present invention clearer, the present invention provides the following drawings for illustration:
图1为本发明的收集系统示意图；Fig. 1 is the collection system schematic diagram of the present invention;
图2为本发明的收集容器的结构示意图。Fig. 2 is a schematic structural view of the collection container of the present invention.
具体实施方式Detailed ways
下面将结合附图，对本发明的优选实施例进行详细的描述。The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
如图1、2所示，关节镜术中髌下脂肪垫组织碎片收集系统，包括关节刨刀2，还包括收集容器3和负压装置，收集容器3包括设置有内部负压腔的外壳34和设置在内部负压腔中的滤筒，外壳34的一端封闭，另一端设置有与内部负压腔连通的出口，出口与负压装置连通，滤筒的进口穿过外壳34的封闭端与关节刨刀2的负压出口连通；所述收集系统上还设置有旁通管路和测压管路，所述旁通管路包括用于连通所述关节刨刀2的负压出口与所述负压装置之间的旁通管8，所述旁通管上设置有用于开闭所述旁通管路的开关阀Ⅰ4，所述旁通管路还包括设置在所述滤筒进口的开关阀Ⅱ6和设置在所述外壳34出口的开关阀Ⅲ7；所述测压管路包括用于连通所述开关阀Ⅱ6的出口与所述开关阀Ⅲ7的进口的连通管9，所述连通管9上设置有用于检测所述滤筒内外压差的压差传感器5及与所述压差传感器5连通的报警器10。实施例中从关节刨刀2到收集容器3再到负压装置是本收集系统的收集通道；从关节刨刀2到开关阀Ⅰ4到负压装置的通道为旁通管路，其主要作用是：第一、在收集过程中更换收集容器3，方便将收集容器3隔离开来方便更换，第二、在收集过程结束时，为了不影响后续的操作通过旁通管路将收集容器3隔离出来，也方便对收集容器3的处理；设置在收集容器3进、出口之间的测量管路的主要作用是检测收集容器3滤筒内、外的压力差，并通过报警器10来提示是否更换收集容器3。As shown in Figures 1 and 2, the infrapatellar fat pad tissue debris collection system during arthroscopic surgery includes a joint planer 2, a collection container 3 and a negative pressure device, and the collection container 3 includes a shell 34 provided with an internal negative pressure chamber and The filter cartridge is arranged in the internal negative pressure chamber, one end of the housing 34 is closed, and the other end is provided with an outlet communicating with the internal negative pressure chamber, the outlet communicates with the negative pressure device, and the inlet of the filter cartridge passes through the closed end of the housing 34 and the joint The negative pressure outlet of the planer 2 is connected; the collection system is also provided with a bypass pipeline and a pressure measurement pipeline, and the bypass pipeline includes a negative pressure outlet for communicating with the joint planer 2 and the negative pressure. A bypass pipe 8 between the devices, the bypass pipe is provided with a switch valve I4 for opening and closing the bypass pipeline, and the bypass pipeline also includes a switch valve II6 arranged at the inlet of the filter cartridge and the on-off valve III7 arranged at the outlet of the casing 34; the pressure measuring pipeline includes a communication pipe 9 for communicating the outlet of the on-off valve II6 with the inlet of the on-off valve III7, and the communication pipe 9 is provided with There is a differential pressure sensor 5 for detecting the differential pressure inside and outside the filter cartridge and an alarm 10 communicating with the differential pressure sensor 5 . In the embodiment, from the joint planer 2 to the collection container 3 and then to the negative pressure device is the collection channel of the collection system; the passage from the joint planer 2 to the switching valve I4 to the negative pressure device is a bypass pipeline, and its main function is: the first One, to replace the collection container 3 during the collection process, it is convenient to isolate the collection container 3 for easy replacement; second, at the end of the collection process, in order not to affect the follow-up operation, the collection container 3 is isolated through the bypass pipeline, and also Facilitate the handling of the collection container 3; the main function of the measuring pipeline arranged between the inlet and outlet of the collection container 3 is to detect the pressure difference inside and outside the filter cartridge of the collection container 3, and to prompt whether to replace the collection container through the alarm 10 3.
本实施例中外壳34和滤筒之间还设置有端盖31。实施例中的端盖31用于连接外壳34和滤筒，方便收集容器3的组装。In this embodiment, an end cover 31 is also provided between the shell 34 and the filter cartridge. The end cap 31 in the embodiment is used to connect the shell 34 and the filter cartridge, so as to facilitate the assembly of the collection container 3 .
本实施例中的端盖31包括盖体和一体设置的具有与滤筒和关节刨刀2的负压出口连通的内部通道的连接体，连接体的一端设置有与滤筒相连的螺纹，另一端设置有与关节刨刀2出口管相连的接口Ⅰ，盖体上设置有与外壳34相连的螺纹。实施例中的端盖采用3D打印机一体成型，加工成本低。The end cap 31 in the present embodiment comprises a cover body and an integrally arranged connecting body having an internal passage communicated with the negative pressure outlet of the filter cartridge and the joint planer 2. One end of the connecting body is provided with a screw thread connected with the filter cartridge, and the other end An interface I connected with the outlet pipe of the joint planer 2 is provided, and a screw thread connected with the shell 34 is provided on the cover. The end cap in the embodiment is integrally formed by a 3D printer, and the processing cost is low.
本实施例中的滤筒包括滤筒架33和滤网35，滤网35为150目金属滤网。实施例中的滤筒采用滤筒架33和滤网35的组合，便于组装；采用150目金属滤网是为了将大多数的脂肪垫碎片收集到滤筒中。The filter cartridge in this embodiment includes a filter cartridge holder 33 and a filter screen 35, and the filter screen 35 is a 150-mesh metal filter screen. The filter cartridge in the embodiment adopts the combination of the filter cartridge frame 33 and the filter screen 35, which is convenient for assembly; the purpose of using a 150-mesh metal filter screen is to collect most of the fat pad fragments into the filter cartridge.
本实施例中的外壳34和端盖31间还设置有密封垫32。实施例中的密封垫32是为了保证负压腔的产生，保证收集容器3的正常运行。In this embodiment, a gasket 32 is also provided between the shell 34 and the end cover 31 . The gasket 32 in the embodiment is to ensure the generation of the negative pressure chamber and ensure the normal operation of the collection container 3 .
本实施例中的负压装置为空气泵1。实施例中的空气泵1为了在收集容器3中产生负压腔，保证收集过程顺利进行。The negative pressure device in this embodiment is the air pump 1 . The air pump 1 in the embodiment is used to generate a negative pressure chamber in the collection container 3 to ensure the smooth progress of the collection process.
本发明还提供一种关节镜术中髌下脂肪垫组织碎片收集系统的收集方法，主要包括以下步骤：The present invention also provides a collection method of the infrapatellar fat pad tissue fragment collection system during arthroscopic surgery, which mainly includes the following steps:
(1)通过打开开关阀Ⅱ6和开关阀Ⅲ7、关闭开关阀Ⅰ4，使收集容器3处于工作状态，旁通管路处于断开状态；(1) By opening the on-off valve II6 and the on-off valve III7, and closing the on-off valve I4, the collection container 3 is in the working state, and the bypass pipeline is in the disconnected state;
(2)使用关节刨刀2对髌下脂肪垫进行抛削，并启动空气泵1，通过空气泵1形成的负压将髌下脂肪垫碎片吸入收集容器3中，同时将冲洗液排出；(2) Use the knuckle planer 2 to polish the infrapatellar fat pad, start the air pump 1, and suck the infrapatellar fat pad fragments into the collection container 3 through the negative pressure formed by the air pump 1, and discharge the washing liquid at the same time;
(3)当报警器10发出报警声时，提示收集容器3的阻力增大，不利于收集，应更换收集容器3，停止抛削和空气泵1，并关闭开关阀Ⅱ6和开关阀Ⅲ7，更换收集容器3；(3) When the alarm 10 sounds an alarm, it indicates that the resistance of the collection container 3 increases, which is not conducive to collection. The collection container 3 should be replaced, the throwing and air pump 1 should be stopped, and the on-off valve II6 and on-off valve III7 should be closed. collection container 3;
(4)再次启动空气泵1，并打开开关阀Ⅱ6和开关阀Ⅲ7，继续抛削、收集；(4) Start the air pump 1 again, and open the on-off valve II6 and the on-off valve III7 to continue throwing and collecting;
(5)抛削结束，关闭开关阀Ⅱ6和开关阀Ⅲ7，打开开关阀Ⅰ4，使收集容器3处于旁通状态，同时，旁通管路处于工作状态，便于后续手术的进行；(5) After finishing the polishing, close the on-off valve II6 and the on-off valve III7, open the on-off valve I4, so that the collection container 3 is in a bypass state, and at the same time, the bypass pipeline is in a working state, which is convenient for subsequent operations;
(6)取下收集容器3，收集结束。(6) Take off the collection container 3, and the collection ends.
最后说明的是，以上优选实施例仅用以说明本发明的技术方案而非限制，尽管通过上述优选实施例已经对本发明进行了详细的描述，但本领域技术人员应当理解，可以在形式上和细节上对其作出各种各样的改变，而不偏离本发明权利要求书所限定的范围。Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that it can be described in terms of form and Various changes may be made in the details without departing from the scope of the invention defined by the claims.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113074104A (en) * | 2021-03-19 | 2021-07-06 | 西安航空学院 | Pressure-limiting overflow device for hydraulic axial plunger pump |
| KR102521122B1 (en) * | 2022-11-22 | 2023-04-11 | 디에스메카 주식회사 | A tissue separation device for biological samples |
| EP4324494A1 (en) | 2022-08-19 | 2024-02-21 | H & B Electronic GmbH & Co. KG | Collecting device |
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| EP4458383A4 (en) * | 2021-12-27 | 2025-08-06 | Miracell Co Ltd | FILTER ARRANGEMENT FOR CARTILAGE SEPARATION |
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| DE102022121041A1 (en) * | 2022-08-19 | 2024-02-22 | H & B Electronic Gmbh & Co. Kg | Collection device |
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