CN107866196B - Conjoined feeding device and method thereof - Google Patents
Conjoined feeding device and method thereof Download PDFInfo
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- CN107866196B CN107866196B CN201711337302.8A CN201711337302A CN107866196B CN 107866196 B CN107866196 B CN 107866196B CN 201711337302 A CN201711337302 A CN 201711337302A CN 107866196 B CN107866196 B CN 107866196B
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000002347 injection Methods 0.000 claims abstract description 154
- 239000007924 injection Substances 0.000 claims abstract description 154
- 238000007789 sealing Methods 0.000 claims abstract description 79
- 230000000903 blocking effect Effects 0.000 claims abstract description 57
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 34
- 240000007643 Phytolacca americana Species 0.000 claims description 10
- 235000009074 Phytolacca americana Nutrition 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000005070 sampling Methods 0.000 claims description 9
- 230000003068 static effect Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 230000006835 compression Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 8
- 108010074051 C-Reactive Protein Proteins 0.000 description 2
- 102100032752 C-reactive protein Human genes 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000008645 C-Reactive Protein (CRP) Assay Methods 0.000 description 1
- 239000001988 antibody-antigen conjugate Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000000562 conjugate Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/24—Stationary reactors without moving elements inside
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J4/00—Feed or outlet devices; Feed or outlet control devices
- B01J4/001—Feed or outlet devices as such, e.g. feeding tubes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- Immunology (AREA)
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- Biomedical Technology (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a conjoined feeding device, which comprises a first injection cylinder, a second injection cylinder, a reaction cup, a sampler, a first hole blocking object and a second hole blocking object; the upper end in the cylinder of the first injection cylinder is provided with a sealing plunger, the lower end of the first injection cylinder is provided with a first plugging object in a sealing way, and the lower end of the sealing plunger is provided with a poking rod; the first injection cylinder can be movably sealed at the upper end in the cylinder of the second injection cylinder, so that the first injection cylinder is a plunger of the second injection cylinder; the lower end of the second injection cylinder is provided with a second hole plugging object in a sealing way; the invention has simple structure, adopts the conjoined injection cylinder structure to ensure that the R1 and R2 reagents can be sprayed to the sampler after being fully mixed, and a specific operation process is only needed to press the sealing plunger for a user, thereby being convenient for reducing the operation cost of personnel and large-scale instruments for reagent detection and improving the efficiency.
Description
Technical Field
The invention belongs to the field of medical reagent detection, and particularly relates to a conjoined supply device and a conjoined supply method.
Background
The diagnosis reagent detection is a common instant diagnosis method, when the reagent detection is adopted, two reagents are often required to be mixed and then added into a sample for reaction, at present, two reagents are often respectively added into a reagent cup through an external feeding device and then added into the sample for reaction, the process needs a large-scale instrument for solving, the instrument needs a liquid path system, a plurality of mechanical arms and a large amount of consumables, hundreds of thousands to millions of materials are input in the early stage, the experiment speed is low, and the instant detection is difficult to realize.
Disclosure of Invention
The invention aims to: in order to overcome the defects in the prior art, the invention provides an integrated one-piece feeding device and a method thereof, wherein the integrated one-piece feeding device is simple in operation process.
The technical scheme is as follows: in order to achieve the above purpose, the integrated supply device of the invention comprises a first injection cylinder, a second injection cylinder, a reaction cup, a sampler, a first hole blocking object and a second hole blocking object;
the upper end in the cylinder of the first injection cylinder is provided with a sealing plunger, the lower end of the first injection cylinder is provided with a first hole plugging object in a sealing way, and the lower end of the sealing plunger is provided with a poking rod;
the first injection cylinder is arranged at the upper end in the cylinder of the second injection cylinder, and the first injection cylinder is sealed and movable in the second injection cylinder, so that the first injection cylinder is a plunger of the second injection cylinder; the lower end of the second injection cylinder is provided with a second hole plugging object in a sealing way;
the second injection cylinder is arranged at the upper end of the reaction cup, the lower end of the second injection cylinder is connected with a sampler, and the lower end of the sampling end of the sampler extends into the reaction cup;
under the action of external force, the sealing plunger moves downwards along with the breaking rod to break the first hole blocking object, and then the sealing plunger moves downwards to the bottom of the first injection cylinder, so that the breaking rod enters the second injection cylinder, and the liquid in the first injection cylinder is injected into the second injection cylinder; then the sealing plunger drives the first injection cylinder and the poking rod to move downwards together, the poking rod pokes the second hole blocking object, and liquid in the second injection cylinder is injected into the reaction cup.
Further, the poking rod is in a pointed column structure.
Further, the tip of the poking rod is arranged at a distance from the first hole blocking object;
in the vertical state of the first injection cylinder, the thickness of the first cavity gas in the first injection cylinder is a, the distance between the tip of the poking rod and the first hole blocking object is b, and a and b meet the requirement of a & gtb; and in the process of pushing the sealing plunger, the thickness of the gas in the first cavity compressed by the sealing plunger is c, so that c is more than b.
Further, the lower end of the inner wall of the second injection cylinder is provided with an injection positioning ring; the first injection cylinder can be displaced to be contacted and clamped with the injection positioning ring;
the lower end of the inner wall of the first injection cylinder is provided with a plunger positioning ring, and the sealing plunger can be displaced to be contacted and clamped with the plunger positioning ring.
Further, the discharge port end of the second injection cylinder is detachably inserted into the reaction cup, so that a mixing cavity is formed between the second hole blocking object and the bottom of the reaction cup.
Further, a cup rim positioning ring is arranged on the outer wall of the second injection cylinder; when the discharge port end of the second injection cylinder is inserted into the reaction cup, the cup opening positioning ring contacts with the cup opening of the reaction cup.
Further, the reaction cup also comprises a sampler and a sampler support, wherein the sampling end of the sampler extends into the reaction cup; the sampling end and the opposite side end of the sampler are connected with a second injection cylinder through the sampler support.
Further, the vertical distance between the tip of the poking rod and the sealing plunger is L, and the vertical distance between the plunger positioning ring and the second hole plugging object is L; satisfy L > L; the thrust required by pushing the sealing plunger to move in the first injection cylinder is F; the thrust required for pushing the first injection cylinder to move in the second injection cylinder is F; satisfying F > F.
Further, a capillary air guide groove (1.1) is vertically formed in the outer side wall of the first injection cylinder (1), the lower end of the capillary air guide groove (1.1) extends to the bottom end of the first injection cylinder (1), and the upper end of the capillary air guide groove (1.1) is spaced from the top end of the first injection cylinder (1).
Further, a method of a one-piece feeding device:
before use or shipment: pre-filling the first cavity in the first injection cylinder and the second cavity in the second injection cylinder with an R reagent and an R reagent respectively;
the user uses: the reaction cup is detached from the second injection cylinder, and the reaction cup is reinstalled after the sampler collects the sample; and then the external mechanical push rod pushes the sealing plunger downwards to enable the sealing plunger to compress air in the first cavity, along with the continuous downward movement of the sealing plunger, the tip end of the poking rod reaches the first hole blocking object and pokes the sealing plunger, the first hole blocking object moves downwards relatively in the second injection cylinder due to compressed gas, R reagent in the first cavity is sprayed into the second cavity through the first hole blocking object which is poked out and fully mixed with R reagent in the second cavity, along with the continuous downward movement of the plunger until the sealing plunger contacts the plunger positioning ring, the sealing plunger and the first injection cylinder start to be relatively stationary under the blocking of the plunger positioning ring, and the mechanical push rod still pushes the sealing plunger downwards due to the continuous downward movement of the mechanical push rod, so that static friction force is overcome between the outer wall of the first injection cylinder and the inner wall of the second injection cylinder, the first injection cylinder moves downwards relatively in the second injection cylinder, and then the first injection cylinder and the sealing plunger synchronously move downwards until the tip end of the poking rod reaches the second hole blocking object and pokes out, the second hole blocking object is fully mixed with the R reagent in the second cavity due to the action of compressed gas, and the mixed reagent in the second cavity is sprayed out from the reaction cup downwards through the second hole blocking object.
Supplementary method before device use or shipment: when the R reagent is filled in the first cavity, part of air needs to be reserved in the first cavity, and the requirement that after the air in the first cavity is compressed by the sealing plunger is met, the stroke of the tip end of the poking rod can reach the first hole blocking object;
the specific numerical relationship is as follows: in the vertical state of the first injection cylinder, the thickness of air in the first cavity is a, the distance between the tip of the poking rod and the first hole blocking object is b, a and b meet a & gtb, the compression ratio of gas cannot be infinitely increased due to the limitation of materials and thrust, the thickness of the gas in the first cavity compressed by the sealing plunger is c, and the thickness of the gas in the first cavity also meets c & gtb, and the mathematical relationship ensures that the stroke of the tip of the poking rod can reach the first hole blocking object;
and (3) the same principle: when the second cavity is filled with the R reagent, the second cavity is also required to be filled with a reserved part of air, so that the tip of the poking rod can reach the position of the second hole plugging object.
The beneficial effects are that: the invention has simple structure, adopts the conjoined injection cylinder structure to ensure that the R1 and R2 reagents can be sprayed to the sampler after being fully mixed, and is convenient for a user to use by only pressing the sealing plunger in the specific operation process, thereby reducing the study cost of reagent detection.
Drawings
FIG. 1 is a schematic view of the present invention in its entirety;
FIG. 2 is a schematic diagram of the overall structure of the present invention prior to use;
FIG. 3 is a schematic view of the internal structure of FIG. 2;
FIG. 4 is a front cross-sectional view of the present device prior to use;
FIG. 5 is a front cross-sectional view of the first hole blocking object after the breaking object has been broken;
fig. 6 is a front cross-sectional view of the breaking object after breaking the second hole blocking object.
Detailed Description
The invention will be further described with reference to the accompanying drawings.
The integrated supply device as shown in fig. 1 to 6 comprises a first injection cylinder 1, a second injection cylinder 9, a reaction cup 12, a sampler 15, a first hole blocking object 4 and a second hole blocking object 14, wherein the first hole blocking object 4 and the second hole blocking object 14 are of a film blocking hole blocking structure in the embodiment; the upper end in the cylinder of the first injection cylinder 1 is provided with a sealing plunger 8, the lower end of the first injection cylinder 1 is provided with a first hole blocking object 4 in a sealing manner, the lower end of the sealing plunger 8 is provided with a poking rod 2, the poking rod 2 in the embodiment is of a pointed column structure, and the tip 17 of the poking rod 2 is arranged at a distance from the first hole blocking object 4; the first injection cylinder 1 is arranged at the upper end in the second injection cylinder 9, and the first injection cylinder 1 is in sealing movement in the second injection cylinder 9, so that the first injection cylinder 1 is a plunger of the second injection cylinder 9; the lower end of the second injection cylinder 9 is provided with a second hole plugging object 14 in a sealing way; the second injection tube 9 is arranged at the upper end of the reaction cup 12, the lower end of the second injection tube 9 is connected with a sampler 15, and the lower end of a sampling end 16 of the sampler 15 extends into the reaction cup 12; the specific operation process comprises the following steps: under the action of external force, the sealing plunger 8 moves downwards along with the breaking rod 2 to break the first hole blocking object 4, so that the sealing plunger 8 continues to move downwards to the bottom of the first injection cylinder 1, the breaking rod 2 enters the second injection cylinder, and the liquid in the first injection cylinder 1 is injected into the second injection cylinder 9; then the sealing plunger 8 drives the first injection cylinder 1 and the poking rod 2 to move downwards together, the poking rod 2 pokes the second hole blocking object 14, and the liquid in the second injection cylinder 9 is injected into the reaction cup 12, so that the specific operation process is only needed to be sampled by an operator or a user, and the sealing plunger is pressed, thereby being convenient for reducing the study cost of reagent detection.
In order to make the tip of the tip 17 of the breaking rod 2 smoothly reach the first hole plugging object 4, the following conditions need to be satisfied when the first injection cylinder 1 is in the vertical state, the gas thickness of the first cavity 11 in the first injection cylinder 1 is a, the distance between the tip 17 of the breaking rod 2 and the first hole plugging object 4 is b, and a and b satisfy a > b; and in the process of pushing the sealing plunger 8, the thickness of the gas in the first cavity 11 compressed by the sealing plunger 8 is c, so that c > b is satisfied.
The lower end of the inner wall of the second injection cylinder 9 is provided with an injection positioning ring 5; the first injection cylinder 1 can be displaced to be in contact with the injection positioning ring 5 for clamping, a plunger positioning ring 3 is arranged at the lower end of the inner wall of the first injection cylinder 1, and the sealing plunger 8 can be displaced to be in contact with the plunger positioning ring 3 for clamping.
The discharge port end of the second injection cylinder 9 is detachably inserted into the reaction cup 12, so that a mixing cavity 7 is formed between the second hole blocking object 14 and the bottom of the reaction cup 12.
The outer wall of the second injection cylinder 9 is provided with a cup opening positioning ring 10; when the discharge port end of the second injection cylinder 9 is inserted into the reaction cup 12, the cup opening positioning ring 10 contacts with the cup opening of the reaction cup 12.
The reaction cup also comprises a sampler 15 and a sampler support 6, wherein a sampling end 16 of the sampler 15 extends into the reaction cup 12; the opposite end of the sampling end 16 of the sampler 15 is supported and connected with the second syringe 9 through the sampler support 6.
The vertical distance between the tip 17 of the poking rod 2 and the sealing plunger 8 is L1, and the vertical distance between the plunger positioning ring 3 and the second hole plugging object 14 is L2; satisfy L2 > L1; the thrust required for pushing the sealing plunger 8 to move in the first injection cylinder 1 is F1; the thrust required for pushing the first syringe 1 to move in the second syringe 9 is F2; in this embodiment, the friction coefficient between the sealing plunger 8 and the first syringe 1 is controlled to be far smaller than the friction coefficient between the first syringe 1 and the second syringe 9, so that when the sealing plunger 8 is pushed, the first syringe 1 and the second syringe 9 do not slide relatively, and after the sealing plunger 8 moves to the plunger positioning ring 3 in the barrel of the first syringe 1 to be blocked and limited, the sealing plunger 8 is pushed to move between the first syringe 1 and the second syringe 9; specific procedures are described below.
The method, the process and the principle of the scheme are as follows:
before use or shipment: prefilling the first cavity 11 in the first syringe 1 and the second cavity 13 in the second syringe 9 with an R1 reagent and an R2 reagent, respectively;
the user uses: the reaction cup 12 is detached from the second injection cylinder 9, and the reaction cup 12 is reinstalled after the sampler 15 collects the sample; then the external mechanical push rod pushes the sealing plunger 8 downwards to enable the sealing plunger 8 to compress air in the first cavity 11, along with the continuous downward movement of the sealing plunger 8, the tip 17 of the poking rod 2 reaches the first hole blocking object 4 and pokes, at the moment of poking, the first hole blocking object 4 is in contact with the compressed air, the R1 reagent in the first cavity 11 is sprayed into the second cavity 13 through the first hole blocking object 4 which is already broken, and is fully mixed with the R2 reagent in the second cavity 13, along with the continuous downward movement of the plunger until the plunger contacts the plunger positioning ring 3, the sealing plunger 8 and the first injection cylinder 1 start to keep relative static under the blocking of the plunger positioning ring 3, and because the mechanical push rod continues to push the sealing plunger 8 downwards, at the moment, the outer wall of the first injection cylinder 1 and the inner wall of the second injection cylinder 9 start to overcome static friction force, the first injection cylinder 1 moves downwards relatively inside the second injection cylinder 9, and the first injection cylinder 1 and the sealing plunger 8 synchronously move downwards until the tip 17 of the poking rod 2 reaches the second hole blocking object 14 and is in contact with the second cavity 13, and the second hole blocking object 14 is broken, and the reagent is sprayed out from the second cavity 14 through the reaction cup 12 which is already in contact with the compressed air.
Supplementary method before device use or shipment: when the first cavity 11 is filled with the R1 reagent, part of air needs to be kept in the first cavity 11, and the requirement that the stroke of the tip 17 of the poking rod 2 can reach the first hole plugging object 4 after the air in the first cavity 11 is compressed by the sealing plunger 8 is met;
the specific numerical relationship is as follows: in the vertical state of the first injection cylinder 1, the thickness of air in the first cavity 11 is a, the distance between the tip 17 of the poking rod 2 and the first hole plugging object 4 is b, a and b meet a & gtb, the compression ratio of gas cannot be infinitely increased due to material and thrust limitation, the thickness of the gas in the first cavity 11 compressed by the sealing plunger 8 is c, and the thickness of the gas also meets c & gtb, and the mathematical relationship ensures that the stroke of the tip 17 of the poking rod 2 can reach the first hole plugging object 4;
and (3) the same principle: when the second cavity is filled with the R2 reagent, it is also necessary to fill the second cavity 13 with a remaining portion of air so that the tip 17 of the breaking bar 2 reaches the second hole stopper 14.
The reagent detection case of this protocol is supplemented with:
product name: c-reactive protein (CRP) assay kit (latex enhanced turbidimetry)
[ test principle ]
CRP antigen in blood is combined with anti-human CRP polyclonal antibody bound on the surface of latex particles, and antigen-antibody reaction occurs to form latex-antibody-antigen conjugate. The turbidity of this conjugate at 630nm was measured, and light was scattered as it passed through the reaction suspension and was detected by a specific protein analyzer, the intensity of scattering being proportional to the content of antigen-antibody immunocomplexes.
[ Main component ]
The device adopts the operation description of a mechanical device: the second syringe is removed from the cuvette and the sample is collected by the collector and reinstalled in the cuvette. Placing the conjoined injection device on an instrument, moving the sealing plunger 8 downwards along with the poking rod 2 to poke the first hole blocking object 4 under the action of the mechanical arm of the instrument, further moving the sealing plunger 8 downwards to the bottom of the first injection cylinder 1, enabling the poking rod to enter the second injection cylinder, and injecting the liquid in the first injection cylinder 1 into the second injection cylinder 9; then the sealing plunger 8 drives the first injection cylinder 1 and the poking rod 2 to move downwards together, the poking rod 2 pokes the second hole blocking object 14, and the liquid in the second injection cylinder 9 is injected into the reaction cup 12. And (5) detecting after the instrument is vibrated and mixed uniformly, and printing a report.
Supplementary explanation of the present embodiment: in this embodiment, as shown in fig. 1, in order to ensure smooth implementation of product assembly, a capillary air guide groove 1.1 is vertically arranged on the outer side wall of the first syringe 1, the lower end of the capillary air guide groove 1.1 extends to the bottom end of the first syringe 1, and the upper end of the capillary air guide groove 1.1 maintains a distance from the top end of the first syringe 1; the purpose is as follows: in the process of assembling the second syringe 9 and the first syringe 1, if the first syringe 1 is not inserted into the second syringe 9, the air in the second cavity 13 is compressed in the process of assembling if the capillary air guide groove 1.1 is not provided, so that a rebound force is formed, when the first syringe 1 is ready to be inserted into the second syringe 9 under the condition of the capillary air guide groove 1.1, the capillary air guide groove 1.1 conducts the outside and the second cavity 13, so that the first syringe 1 can be smoothly inserted, and meanwhile, with the further insertion of the first syringe 1, the upper end of the capillary air guide groove 1.1 is kept at a distance from the top end of the first syringe 1 until the first syringe 1 is completely inserted into the second syringe 9, so that the capillary air guide groove 1.1 loses the effect of balancing air pressure, and the second cavity 13 is completely sealed;
in addition, the scheme is not limited to the arrangement of the capillary air guide groove 1.1 to realize the smooth implementation of the assembly process; in the product assembling process, the scheme can also ensure that the inserting process of the first injection cylinder 1 can be smoothly implemented by not sealing the second hole plugging object 14 at the lower end of the second injection cylinder 9 before the first injection cylinder 1 is ready to be inserted into the second injection cylinder 9; and after the first injection cylinder 1 is completely inserted, sealing the second hole plugging object 14 at the lower end of the second injection cylinder 9.
The foregoing is only a preferred embodiment of the invention, it being noted that: it will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present invention, and such modifications and adaptations are intended to be comprehended within the scope of the invention.
Claims (6)
1. A conjoined feeding apparatus, characterized in that: the device comprises a first injection cylinder (1), a second injection cylinder (9), a reaction cup (12), a sampler (15), a first hole blocking object (4) and a second hole blocking object (14);
the upper end in the cylinder of the first injection cylinder (1) is provided with a sealing plunger (8), the lower end of the first injection cylinder (1) is provided with a first hole blocking object (4) in a sealing manner, and the lower end of the sealing plunger (8) is provided with a poking rod (2);
the first injection cylinder (1) is arranged at the upper end in the cylinder of the second injection cylinder (9), and the first injection cylinder (1) is sealed and movable in the second injection cylinder (9), so that the first injection cylinder (1) is a plunger of the second injection cylinder (9); the lower end of the second injection cylinder (9) is provided with a second hole blocking object (14) in a sealing manner, the second injection cylinder (9) is arranged at the upper end of the reaction cup (12), the lower end of the second injection cylinder (9) is connected with a sampler (15), and the lower end of a sampling end (16) of the sampler (15) extends into the reaction cup (12);
under the action of external force, the sealing plunger (8) moves downwards along with the poking rod (2) to poke the first hole blocking object (4), so that the sealing plunger (8) continues to move downwards to the bottom of the first injection cylinder (1), the poking rod (2) enters the second injection cylinder, and liquid in the first injection cylinder (1) is injected into the second injection cylinder (9); then the sealing plunger (8) drives the first injection cylinder (1) and the poking rod (2) to move downwards together, the poking rod (2) pokes the second hole blocking object (14), and liquid in the second injection cylinder (9) is injected into the reaction cup (12);
the poking rod (2) is of a pointed column structure; the tip (17) of the poking rod (2) is arranged at a distance from the first hole blocking object (4); in the vertical state of the first injection cylinder (1), the gas thickness of a first cavity (11) in the first injection cylinder (1) is a, the distance between the tip (17) of the poking rod (2) and the first hole blocking object (4) is b, and a and b meet a & gtb; in the process of pushing the sealing plunger (8), the thickness of the gas in the first cavity (11) compressed by the sealing plunger (8) is c, and c is more than b;
an injection positioning ring (5) is arranged at the lower end of the inner wall of the second injection cylinder (9); the first injection cylinder (1) can be displaced to be clamped in contact with the injection positioning ring (5);
the lower end of the inner wall of the first injection cylinder (1) is provided with a plunger positioning ring (3), and the sealing plunger (8) can be displaced to be contacted and clamped with the plunger positioning ring (3);
the vertical distance between the tip (17) of the poking rod (2) and the sealing plunger (8) is L1, and the vertical distance between the plunger positioning ring (3) and the second hole plugging object (14) is L2; satisfy L2 > L1; the thrust required by pushing the sealing plunger (8) to move in the first injection cylinder (1) is F1; the thrust required for pushing the first injection cylinder (1) to move in the second injection cylinder (9) is F2; satisfies F2 > F1.
2. A conjoined feeding apparatus according to claim 1, wherein: the discharge port end of the second injection cylinder (9) is detachably inserted into the reaction cup (12), so that a mixing cavity (7) is formed between the second hole blocking object (14) and the cup bottom of the reaction cup (12).
3. A conjoined feeding apparatus according to claim 2, wherein: the outer wall of the second injection cylinder (9) is provided with a cup opening positioning ring (10); when the discharge port end of the second injection cylinder (9) is inserted into the reaction cup (12), the cup opening positioning ring (10) contacts with the cup opening of the reaction cup (12).
4. A conjoined feeding apparatus according to claim 3, wherein: the reaction cup also comprises a sampler (15) and a sampler support (6), wherein a sampling end (16) of the sampler (15) stretches into the reaction cup (12); the opposite side end of a sampling end (16) of the sampler (15) is in supporting connection with the second injection cylinder (9) through the sampler support (6).
5. The one-piece feeding device according to claim 4, wherein: the novel injection device is characterized in that a capillary air guide groove (1.1) is vertically formed in the outer side wall of the first injection cylinder (1), the lower end of the capillary air guide groove (1.1) extends to the bottom end of the first injection cylinder (1), and the upper end of the capillary air guide groove (1.1) is spaced from the top end of the first injection cylinder (1).
6. The method of operation of a one-piece feeding device of claim 5, wherein:
before use or shipment: pre-filling a first cavity (11) in the first syringe (1) and a second cavity (13) in the second syringe (9) with an R1 reagent and an R2 reagent respectively;
the user uses: removing the reaction cup (12) from the second injection cylinder (9), and reinstalling the reaction cup (12) after the sampler (15) collects the sample; then the external mechanical push rod pushes the sealing plunger (8) downwards to enable the sealing plunger (8) to compress air in the first cavity (11), along with the continuous downward movement of the sealing plunger (8), the tip (17) of the poking rod (2) reaches the first hole blocking object (4) and pokes, the R1 reagent in the first cavity (11) is sprayed into the second cavity (13) through the broken first hole blocking object (4) due to compressed air at the moment of poking, and is fully mixed with the R2 reagent in the second cavity (13), along with the continuous downward movement of the plunger until the sealing plunger (8) and the first injection cylinder (1) start to be kept relatively static under the blocking of the plunger positioning ring (3), the first injection cylinder (1) and the second injection cylinder (9) start to overcome static friction force at the moment when the outer wall of the first injection cylinder (1) is continuously pushed downwards, the first injection cylinder (1) moves downwards in the second injection cylinder (9) relatively until the tip (14) is broken due to the continuous downward movement of the plunger positioning ring (3), and the sealing plunger (8) starts to be simultaneously broken until the tip (14) and the sealing plunger (1) moves downwards until the tip (14) is stopped synchronously, the mixed reagent in the second cavity (13) is sprayed into the reaction cup (12) below through the broken second hole blocking object (14) and reacts with the sample;
supplementary method before device use or shipment: when the first cavity (11) is filled with the R1 reagent, part of air needs to be reserved in the first cavity (11), and the requirement that after the air in the first cavity (11) is compressed by the sealing plunger (8), the stroke of the tip (17) of the poking rod (2) can reach the first hole blocking object (4) is met;
the specific numerical relationship is as follows: in the vertical state of the first injection cylinder (1), the air thickness in the first cavity (11) is a, the distance between the tip (17) of the poking and breaking rod (2) and the first hole blocking object (4) is b, a and b meet a & gtb, the compression ratio of gas cannot be infinitely increased due to material and thrust limitation, the compressed thickness of the gas in the first cavity (11) by the sealing plunger (8) is c, and the c & gtb is met, and the mathematical relationship ensures that the stroke of the tip (17) of the poking and breaking rod (2) can reach the first hole blocking object (4);
and (3) the same principle: when the second cavity is filled with the R2 reagent, the second cavity (13) is also required to be filled with a reserved part of air, so that the tip (17) of the poking rod (2) can reach the position of the second hole blocking object (14).
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| CN108531377A (en) * | 2018-06-26 | 2018-09-14 | 广东省第二人民医院（广东省卫生应急医院） | It is a kind of can pre-filled reagent diaphragm type nucleic acid amplification airtight reactor tube |
| CN109868216A (en) * | 2019-04-01 | 2019-06-11 | 丹娜（天津）生物科技有限公司 | A kind of fungi 1-3- β-D glucan detection kit of simplification |
| CN109925966B (en) * | 2019-04-01 | 2024-09-10 | 丹娜(天津)生物科技股份有限公司 | Reagent filling and reaction device |
| CN110102242B (en) * | 2019-06-12 | 2023-09-05 | 成都恩普生医疗科技有限公司 | Reaction cup integrating packaging and mixing method |
| MX2022003405A (en) * | 2019-09-20 | 2022-08-22 | Meiragtx Therapeutics Inc | Injection systems and methods of their use. |
| CN113188865B (en) * | 2021-04-29 | 2022-11-04 | 河南科技大学第一附属医院 | A pre-processing device for fecal sample detection in intensive care unit |
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