CN107986872A - A kind of slow-release bio bacterial manure, preparation method and its application in salt affected soil fertilising - Google Patents

Abstract

The invention relates to a slow-release biological bacterial fertilizer, a preparation method and its application in saline-alkali soil fertilization, and belongs to the technical field of biological bacterial fertilizer. The biological bacterial fertilizer uses bean dregs as a starting material, and after hydrolysis and fermentation, the plant fiber and protein in it can be effectively used as raw materials for slow-release fertilizers, and polymerized with polymer controlled-release components to form a three-dimensional Slow-release fertilizer particles with a network structure; on the one hand, the biological bacterial fertilizer realizes the reuse of tofu processing waste, and on the other hand, realizes the use effect of preparing high-efficiency slow-release fertilizers. When the slow-release fertilizer is applied to soil in saline-alkaline areas, It has good slow-release effect and good soil improvement.

Description

A slow-release biological bacterial fertilizer, its preparation method and its application in saline-alkali soil fertilization

technical field

The invention relates to a slow-release biological bacterial fertilizer, a preparation method and its application in saline-alkali soil fertilization, and belongs to the technical field of biological bacterial fertilizer.

Background technique

During the formation of the plain, a large amount of salt in the river water remained in the parent material of the plain and the water body of the groundwater. In addition, the particles of the parent material were fine and dense, resulting in poor drainage of the groundwater and shallow buried depth. Under the action of evaporation, the shallow underground The layer of water is transported to the surface through capillary tubes to be evaporated. During the process of capillary transporting water to the surface, the salt in the water is also brought to the surface. After the water is evaporated, the salt remains on the surface and the shallow soil on the ground. Soil salinization occurs when there is too much salt and there is not enough fresh water to dilute it and drain it away.

Salinization is widely distributed in my country. Except for the saline soil in coastal semi-humid areas, most of them are distributed in the arid and semi-arid regions along the Huaihe River-Qinling Mountains-Bayan Harshan-Tanggula Mountain line, that is, north of 33 degrees north latitude. area. According to the available data, saline soil is distributed in 18 provinces and autonomous regions of the country, with a total area of about 818,000 km ² , accounting for 8.5% of the national area, of which modern saline soil accounts for about 45% (369,300 km ² ), and the remaining (historical 448,700 km ² of saline soil formed above, and 173,300 km ² of potential saline soil (higher salt content meets the conditions for formation).

At present, the application of microbial remediation technology in environmental protection and soil remediation is mainly through the continuous utilization, degradation, and transformation of environmental pollution and damage factors by specific microorganisms, so that the contaminated and damaged soil can be safely and long-term repaired. Microorganisms are decomposers in natural ecosystems. They can continuously degrade various pollutants entering the environment, and finally convert them into inorganic substances such as CO ₂ and H ₂ O, purifying the polluted environment. Microbial treatment technology is not only suitable for large-scale pollution treatment, but also can use natural water or soil as a pollutant treatment site, thereby greatly saving the cost of treatment. It is fully foreseeable that in the 21st century, microbial remediation technology will become the most valuable and vital engineering technology of choice in the field of ecological and environmental protection around the world. Soil contains a lot of nutrients needed by microorganisms, such as C, H, O, N, P, S, etc. The growth of microbial cells requires a large amount of Na ⁺ and K ⁺ to maintain the absorption and transportation of nutrients by cells. That is to say, through the growth and reproduction of microorganisms, the saline-alkali components in the saline-alkali soil can be decomposed, transformed, absorbed and utilized, and the soluble salt content in the soil can be greatly reduced so as to restore the normal saline-alkali content of the soil and achieve the purpose of improving saline-alkali land.

CN102344814A discloses a method for preparing a soil conditioner for improving soil salinity. The saline-alkaline soil conditioner is prepared by combining Rhodopseudomonas palustris, Pichia membranosa, Lactobacillus casei, Streptomyces luteus Mixed with seaweed slurry, through multiple fermentations, a variety of beneficial microorganisms and seaweeds are organically combined, and a new type of microbial active agent-seaweed biological bacteria is formed by compound culture, and an appropriate amount of humic acid, organic matter, and active zeolite powder are added. And molasses, after fermentation, the fermentation product is dried, through the strong effect of unique ion adsorbent, organic acid-base neutralization balancer, ion conversion agent and beneficial bacteria transformation starter, to improve the soil environment, adjust the acid-base balance, The purpose of reducing salt content can effectively improve soil fertility and air permeability, loosen soil, eliminate compaction, degrade harmful substances, and promote the accumulation and transformation of organic matter. CN101560394A discloses a conditioner for saline-alkali soil, which is formed by mixing calcium powder, ammonium dihydrogen phosphate, humic acid and fermented organic fertilizer. This conditioner can reduce soil bulk density, increase cation exchange capacity, increase organic matter, and prevent microbial Diversity increased and vegetation recovered. CN104962288A discloses a soil bio-improvement agent for saline-alkali land, which comprises crushing corn stalks and cotton stalks; mixing peat manure, animal manure, bean dregs, distiller's grains, and vinegar dregs in a volume ratio of 1:1:1:1:1 to obtain an organic fertilizer; uniformly mixing the pulverized product of corn stalks and cotton stalks, the obtained organic waste and compound fertilizer according to the weight ratio of 65%-78%: 20%-30%: 2%-10%; adding water to the obtained mixture It is sealed and fermented, and sterilized during the decomposing process; when the volume of the mixture is reduced by 1/2, it is applied to the saline-alkali soil, which not only improves the soil fertility, improves the soil structure, reduces the evaporation of surface water, and inhibits the return of salt, but also benefits The survival of microorganisms is beneficial to the life of ground cover plants and improves the ecological environment of saline-alkali land.

However, the adjustment effect of the above-mentioned improvers is easy to be affected by rainfall, water flow, etc., and the effect of microbial treatment of saline-alkali land cannot be found for a long time. Therefore, it is necessary to develop a biological fertilizer with a slow-release effect.

Contents of the invention

The object of the present invention is: to propose a novel composite slow-release biological fertilizer, which utilizes bean dregs as a starting material, and can effectively utilize plant fiber and plant protein therein as a slow-release fertilizer after hydrolysis and fermentation. raw materials, and polymerize them with polymer controlled-release components to form slow-release fertilizer particles with a three-dimensional network structure; when the slow-release fertilizer is applied to soil in saline-alkaline areas, it has good slow-release effect and good soil improvement .

The technical solution is:

A first aspect of the present invention provides:

A slow-release biological bacterial fertilizer, which is prepared from hydrolyzed and fermented bean dregs, polymers, attapulgite, EM bacterial liquid, nitrogen fertilizer, phosphate fertilizer, potassium fertilizer, humic acid, and CaSO ₄ as raw materials.

In one embodiment, the nitrogen fertilizer is urea.

In one embodiment, the phosphate fertilizer is ammonium dihydrogen phosphate.

In one embodiment, the potassium fertilizer is potassium sulfate.

A second aspect of the present invention provides:

A preparation method for slow-release biological bacterial fertilizer, comprising the steps of:

Step 1, hydrolysis of bean dregs: by weight, take 10-12 parts of bean dregs, wash with water, add bean dregs to 500-600 parts of water, then add cellulase, and use dilute acid to adjust the pH to 4-6 , carry out the cellulose hydrolysis reaction, and then deactivate the enzyme treatment;

In the second step, after the hydrolyzate of the first step is concentrated under reduced pressure, the bacterial liquid is added for fermentation treatment, and after vacuum drying, the bean dregs fermented product is obtained;

Step 3, the preparation of the water phase: by weight, 20-35 parts of bean dregs fermented product, 400-500 parts of water, 1-4 parts of anionic surfactant, 4-8 parts of emulsifier, methyl methacrylate 10-12 parts, 15-20 parts of methacrylic acid, 3-5 parts of chitosan, 2-4 parts of N-isopropylacrylamide, 0.2-0.5 parts of acetic acid, 1-3 parts of initiator, and water Mutually;

Step 4, preparation of the oil phase: in parts by weight, mix toluene and chlorobenzene at a weight ratio of 1:1 to 1.2, then add 0.1 to 0.2% surfactant by weight of the mixture, and mix well to obtain the oil phase;

Step 5, reversed-phase suspension polymerization: After mixing the water phase and the oil phase according to the volume ratio of 1:3~6, blow nitrogen into the reactor, and then add N,N,N',N'-tetramethylethane Diamine, reflux reaction under nitrogen conditions; after the reaction, the solid matter is filtered out and cleaned with ethanol to obtain a granular slow-release fertilizer base material;

Step 6, by weight, mix 40-50 parts of granular slow-release fertilizer base material, 20-25 parts of attapulgite, 4-8 parts of EM bacteria solution, and then mix with 2-4 parts of urea, diphosphate Mix 4-6 parts of ammonium hydrogen, 5-8 parts of potassium sulfate, 10-12 parts of humic acid, and 5-8 parts of CaSO ₄ , mix them evenly, and granulate them.

In one embodiment, the dilute acid in the first step refers to 1-5wt% dilute hydrochloric acid, the amount of cellulase added is 0.05-0.5% of the weight of bean dregs, and the cellulase is green wood enzyme, which hydrolyzes The reaction time is 12 to 24 hours, and the reaction temperature is 40 to 45°C; the enzyme inactivation treatment refers to the high temperature above 95°C to inactivate the enzyme.

In one embodiment, the concentration in the second step means that the volume is concentrated to the original 20-30%, the fermentation temperature is 30-35°C, and the fermentation time is 40-80h; the bacterial liquid refers to the fermentation of Saccharomyces cerevisiae and Bacillus subtilis bacteria liquid.

In one embodiment, the anionic surfactant described in the 3rd step is selected from alkylbenzene sulfonate; Emulsifier is selected from at least one in sucrose ester, soybean lecithin and monoglyceride; Initiator is Potassium sulfate.

In one embodiment, the surfactant in step 4 is SPAN-40.

In one embodiment, the reflux reaction in step 5 is carried out at 75-85° C. for 2-4 hours.

A third aspect of the present invention provides:

Application of the above-mentioned slow-release biological bacterial fertilizer in saline-alkali soil fertilization.

In one embodiment, corn is planted in the saline-alkaline soil.

Beneficial effect

1) The present invention utilizes bean dregs as a raw material, through hydrolysis and fermentation of the more plant fibers and protein contained in the bean dregs, the cellulose is hydrolyzed to produce more sugars and fibers containing hydroxyl groups, and at the same time through The protein is decomposed into small molecular proteins, peptides, etc. by fermentation. These fibers containing a large number of hydroxyl groups and small molecular proteins and peptides containing more amino groups can easily form an interpenetrating network with the polyacrylic acid main chain in the reverse-phase polymerization reaction. , a fiber-polymer composite slow-release base material with a large spatial network structure, which has a large internal space area, can provide slow-release fertilizers and provide sufficient space for the growth of microorganisms, and the slow-release base There are macromolecular proteins linked to the material, which can further provide nutrients for microbial activities, and gradually release fertilizers such as amino acids metabolized by the bacterial fluid, which improves the slow release of nitrogen fertilizers and makes the fertilizers more durable;

2) In the present invention, the microbial activity of the EM bacterial liquid in the slow-release base material is used to continuously produce nutrients that can promote root growth, and the EM bacterial liquid can produce more metabolic amino acids, so that the slow-release fertilizer base material It can form an acidic environment, adjust soil pH with acid, neutralize alkalinity, activate nutrients in soil and fertilizers, change the soil structure of saline-alkali land, and destroy the conditions for salt to rise along capillary pores; promote Ca ²⁺ in CaSO ₄ and soil The exchanged Na ⁺ undergoes a substitution reaction, and the formed sodium sulfate is washed out with water, which enhances soil salt discharge, and at the same time promotes soil flocculation and better water permeability.

3) The long chain of chitosan molecules added to the slow-release fertilizer base material in the present invention contains amino groups, hydroxyl groups, and undeacylated acetamido groups, which can react with nitrogen, oxygen, etc. in macromolecules and peptides after soybean protein fermentation Form complexes through hydrogen bonds, shuttle in the network structure to form a semi-interpenetrating network, further increase the specific surface area of the slow-release fertilizer gene, and promote the activity of microorganisms.

4) When the slow-release fertilizer provided by the present invention is applied to saline-alkali land, it can help alleviate soil compaction in saline-alkali land, neutralize acidity and alkalinity, promote soil aggregation, increase organic carbon content in soil, and promote plant growth.

Description of drawings

Figure 1 is a graph showing the change of soil total nitrogen over time in different experimental groups;

Fig. 2 is the measurement result figure of soil respiration rate in different experimental groups;

Fig. 3 is the determination result figure of organic carbon content in soil in different experimental groups;

Fig. 4 is a graph showing the measurement results of sodium salt content in soil in different experimental groups.

Detailed ways

The present invention will be further described in detail through specific embodiments below. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

Approximate terms used herein may be used throughout the specification and claims to modify any number of expressions, which permissible changes would result in a change in the basic function to which it is related. Accordingly, a value modified by a term such as "about" is not to be limited to the precise value specified. In at least some cases, the approximation may correspond to the precision of the instrument used to measure the value. Unless context or language dictates otherwise, range limitations may be combined and/or interchanged, and such ranges are identified to include all the subranges included herein. Except where indicated in the working examples or elsewhere, all numbers or expressions indicating amounts of ingredients, reaction conditions, etc. used in the specification and claims are to be understood in all cases as being protected by the word "about " Modification.

Values expressed in range format should be understood in a flexible manner to include not only the values explicitly recited as the limits of the range, but also all individual values or subranges encompassed within that range, as if each value and subrange were expressly List out. For example, a concentration range of "about 0.1% to about 5%" should be understood to include not only the explicitly recited concentrations of about 0.1% to about 5%, but also individual concentrations within the indicated range (e.g., 1%, 2%, %, 3% and 4%) and subranges (for example, 0.1% to 0.5%, 1% to 2.2%, 3.3% to 4.4%).

As used herein, the words "comprises," "comprising," "having," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, article, or apparatus comprising listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or inherent to the process, method, article, or apparatus.

The percentages mentioned in the present invention all refer to mass percentages unless otherwise specified.

In the present invention, the bean dregs is a by-product in the process of producing bean curd, which is a solid residue obtained after pressure filtration, and has various nutrients such as protein, fat, calcium, phosphorus, and iron. The production and sales of tofu in my country are relatively large, and the corresponding output of bean dregs is also large. Okara contains more plant fiber and plant protein, which can be used as organic fertilizer.

Trichoderma viride (No. GIM3.141), Saccharomyces cerevisiae (No. GIM2.139) and Bacillus subtilis ( No. GIM1.135) used in the present invention were purchased from Guangdong Microbial Culture Collection Center to buy. The EM stock solution was purchased from Aimule Environmental Biotechnology (Nanjing) Co., Ltd., which contains more than 80 kinds of effective and active microorganisms such as photosynthetic bacteria, lactic acid bacteria, and yeasts. The number of viable bacteria is ≥100 million/ml, pH ≥ 3.8, and the color is yellow Brown, translucent liquid with a strong sour or sour smell.

The invention provides a slow-release biological bacterial fertilizer, which is made of bean dregs, high molecular polymers, attapulgite, EM bacterial liquid, nitrogen fertilizer, phosphate fertilizer, potassium fertilizer, humic acid and _CaSO4 which have been treated by hydrolysis and fermentation as raw materials Prepared.

Among the above-mentioned raw materials, soybean dregs contain more plant fiber and plant protein. First, hydrolyze and ferment the cellulose to produce more sugars and fibers containing hydroxyl groups. At the same time, the protein is fermented. Decompose into small molecular proteins, peptides, etc. These fibers containing a large number of hydroxyl groups and small molecular proteins and peptides containing more amino groups make covalent bonds between proteins, peptide molecules and monomers to obtain a three-way cross-linking network Frame structure, interpenetrating network with polyacrylic acid main chain, fiber-polymer composite slow-release base material with large spatial network structure, which has a large internal space area, can provide slow-release fertilizer and provide microorganisms The growth of the slow-release base material provides sufficient space, and the slow-release base material is linked with macromolecular proteins, which can further provide nutrients for microbial activities, gradually release fertilizers such as amino acids metabolized by the bacterial liquid, improve the slow-release performance of nitrogen fertilizers, and make fertilizers more effective more durable. The EM bacteria solution plays a role in microbial activity in the slow-release base material, continuously producing nutrients that can promote root growth, and the EM bacteria solution can produce more metabolized amino acids, so that an acidic environment can be formed near the slow-release fertilizer base material, and acid regulation Soil pH, neutralizing alkalinity, activating nutrients in soil and fertilizers, changing the soil structure of saline-alkali land, destroying the conditions for the salt to rise along capillary pores; promoting the replacement of Ca ²⁺ in CaSO ₄ with exchangeable Na ⁺ in the soil Reaction, the formed sodium sulfate is washed out with water, which enhances soil salt discharge, and at the same time promotes soil flocculation and better water permeability. When slow-release fertilizer is applied to saline-alkali land, it can help alleviate soil compaction in saline-alkali land, neutralize acidity and alkalinity, promote soil aggregation, increase organic carbon content in soil, and promote plant growth.

In one embodiment, the nitrogen fertilizer can be urea; the phosphate fertilizer can be ammonium dihydrogen phosphate; and the potassium fertilizer can be potassium sulfate.

The present invention also provides the preparation method of above-mentioned slow-release fertilizer, and the steps are mainly:

Step 1, hydrolysis of bean dregs: by weight, take 10-12 parts of bean dregs, wash with water, add bean dregs to 500-600 parts of water, then add cellulase, and use dilute acid to adjust the pH to 4-6 , carry out cellulose hydrolysis reaction, and then inactivate enzyme treatment; in this step, mainly utilize cellulase to carry out enzymatic hydrolysis to soybean plant fiber, can effectively decompose soybean plant fiber, make it produce more sugar, and More hydroxyl groups are produced on the fiber, and subsequent cross-linking reactions are utilized; in one embodiment, dilute acid refers to 1-5wt% dilute hydrochloric acid, and the amount of cellulase added is 0.05-0.5% of the weight of bean dregs, so The above-mentioned cellulase is green wood enzyme, the hydrolysis reaction time is 12-24 hours, and the reaction temperature is 40-45° C.; the enzyme-killing treatment refers to high-temperature enzyme killing above 95° C.

In the second step, after the hydrolyzate of the first step is concentrated under reduced pressure, the bacterial liquid is added for fermentation treatment, and after vacuum drying, the bean dregs fermented product is obtained; in this step, the bean dregs after enzymatic hydrolysis is fermented, Utilizing the action of microorganisms, macromolecular proteins can be decomposed to obtain small molecular proteins and amino acids, which can effectively construct a three-dimensional interpenetrating network of protein-cellulose-polyacrylic acid, and can provide fermentation substrates for microorganisms to produce plant growth. Nitrogen source; in one embodiment, concentration means that the volume is concentrated to 20-30% of the original, the fermentation temperature is 30-35°C, and the fermentation time is 40-80h; the bacterial liquid refers to the bacterial liquid of Saccharomyces cerevisiae and Bacillus subtilis .

Step 3, the preparation of the water phase: by weight, 20-35 parts of bean dregs fermented product, 400-500 parts of water, 1-4 parts of anionic surfactant, 4-8 parts of emulsifier, methyl methacrylate 10-12 parts, 15-20 parts of methacrylic acid, 3-5 parts of chitosan, 2-4 parts of N-isopropylacrylamide, 0.2-0.5 parts of acetic acid, 1-3 parts of initiator, and water Phase; In one embodiment, described anionic surfactant is selected from alkylbenzene sulfonate; Emulsifier is selected from at least one in sucrose ester, soybean lecithin and monoglyceride; Initiator is potassium persulfate . The added methyl methacrylate, the added methacrylic acid and N-isopropylacrylamide are monomers used as the main chain molecules of the copolymerization network, and the added chitosan molecule long chain contains amino groups, hydroxyl groups and undesorbed The acetylamino group of acyl can form complexes with nitrogen and oxygen in macromolecules and peptides after fermentation of soybean protein through hydrogen bonds, and shuttle in the network structure to form a semi-interpenetrating network, which further improves the ratio of slow-release fertilizer genes. surface area, which facilitates the activity of microorganisms.

Step 4, preparation of the oil phase: in parts by weight, mix toluene and chlorobenzene at a weight ratio of 1:1 to 1.2, then add 0.1 to 0.2% surfactant by weight of the mixture, and mix well to obtain the oil phase; In one embodiment, the surfactant is SPAN-40.

Step 5, reversed-phase suspension polymerization: After mixing the water phase and the oil phase according to the volume ratio of 1:3~6, blow nitrogen into the reactor, and then add N,N,N',N'-tetramethylethane Diamine, reflux reaction under nitrogen; after the reaction, the solid matter was filtered out and washed with ethanol to obtain a granular slow-release fertilizer base material; through the third and fourth steps, what was obtained was the inverse suspension polymerization. The water phase and the oil phase, under the action of the initiator, the fibers, proteins, monomers, and chitosan in the water phase will build a three-dimensional interpenetrating network, and then after washing and purification, the slow-release base material will be obtained. In one embodiment, the reflux reaction is carried out at 75-85° C. for 2-4 hours.

Step 6, by weight, mix 40-50 parts of granular slow-release fertilizer base material, 20-25 parts of attapulgite, 4-8 parts of EM bacteria solution, and then mix with 2-4 parts of urea, diphosphate Mix 4-6 parts of ammonium hydrogen, 5-8 parts of potassium sulfate, 10-12 parts of humic acid, and 5-8 parts of CaSO ₄ , mix them evenly, and granulate them. In this step, first, the granular slow-release fertilizer base material and attapulgite are used as the carrier of the fertilizer. After mixing with the EM bacterial liquid, the microorganisms in the EM bacterial liquid are firstly allowed to enter the inner pores of the carrier. Next, compound organic fertilizer is obtained by mixing with other components of the fertilizer.

Embodiment 1 Preparation of slow-release biological bacterial fertilizer

The first step, the hydrolysis of bean dregs: by weight, take 10 parts of bean dregs, after washing with water, add bean dregs to 500 parts of water, then add green wood enzyme, the amount of green wood enzyme added is 0.05% of the weight of bean dregs, and use 1wt% dilute hydrochloric acid to adjust the pH to 4-6, and carry out the hydrolysis reaction of cellulose. The hydrolysis reaction time is 12 hours, the reaction temperature is 40°C, and the enzyme is killed at a high temperature above 95°C;

In the second step, the hydrolyzate of the first step is concentrated under reduced pressure to 20% of the original volume, and then the bacterial liquid of Saccharomyces cerevisiae and Bacillus subtilis is added for fermentation treatment. The fermentation temperature is 30°C, the fermentation time is 40h, and then vacuum dried. , to obtain fermented bean dregs;

The third step, the preparation of the water phase: by weight, 20 parts of fermented bean dregs, 400 parts of water, 1 part of sodium octadecylbenzenesulfonate, 4 parts of monoglycerides, and 10 parts of methyl methacrylate , 15 parts of methacrylic acid, 3 parts of chitosan, 2 parts of N-isopropylacrylamide, 0.2 part of acetic acid, mixed evenly, and 1 part of potassium persulfate to obtain the aqueous phase;

Step 4, preparation of the oil phase: in parts by weight, mix toluene and chlorobenzene at a weight ratio of 1:1, then add 0.1% SPAN-40 by weight of the mixture, and mix well to obtain the oil phase;

Step 5, reversed-phase suspension polymerization: After mixing the water phase and the oil phase according to the volume ratio of 1:3, nitrogen gas is introduced into the reactor, and then N,N,N',N'-tetramethylethylenediamine is added , and reacted at 75°C under nitrogen for 2 hours; after the reaction, the solid matter was filtered out and washed with ethanol to obtain a granular slow-release fertilizer base material;

The 6th step, by weight, 40 parts of granular slow-release fertilizer base material, 20 parts of attapulgite, 4 parts of EM bacteria liquid are mixed uniformly, then with 2 parts of urea, 4 parts of ammonium dihydrogen phosphate, 5 parts of potassium sulfate 10 parts, 10 parts of humic acid, 5 parts of CaSO ₄ are mixed evenly, and after granulation, it is obtained.

Example 2

The first step, the hydrolysis of bean dregs: by weight, take 12 parts of bean dregs, after washing with water, add bean dregs to 600 parts of water, then add green wood enzyme, the amount of green wood enzyme added is 0.5% of the weight of bean dregs, and use 5wt% dilute hydrochloric acid to adjust the pH to 4-6, and carry out the cellulose hydrolysis reaction. The hydrolysis reaction time is 24 hours, the reaction temperature is 45°C, and the enzyme is killed at a high temperature above 95°C;

In the second step, the hydrolyzate in the first step is concentrated under reduced pressure to 30% of its original volume, and then the bacterial liquid of Saccharomyces cerevisiae and Bacillus subtilis is added for fermentation treatment. The fermentation temperature is 35°C, the fermentation time is 80h, and then vacuum dried. , to obtain fermented bean dregs;

Step 3, the preparation of the water phase: by weight, 35 parts of fermented bean dregs, 500 parts of water, 4 parts of sodium octadecylbenzenesulfonate, 8 parts of monoglycerides, and 12 parts of methyl methacrylate , 20 parts of methacrylic acid, 5 parts of chitosan, 4 parts of N-isopropylacrylamide, 0.5 part of acetic acid, mixed evenly, and 3 parts of potassium persulfate to obtain the water phase;

Step 4, the preparation of the oil phase: by weight, mix toluene and chlorobenzene according to the weight ratio of 1: 1.2, then add 0.2% SPAN-40 by weight of the mixture, and mix well to obtain the oil phase;

Step 5, reversed-phase suspension polymerization: After mixing the water phase and the oil phase according to the volume ratio of 1:6, nitrogen gas is introduced into the reactor, and then N,N,N',N'-tetramethylethylenediamine is added , and reacted at 85° C. for 4 hours under nitrogen; after the reaction, the solid matter was filtered out and washed with ethanol to obtain a granular slow-release fertilizer base material;

The 6th step, by weight, mix 50 parts of granular slow-release fertilizer base material, 25 parts of attapulgite, 8 parts of EM bacterial liquid, then mix with 4 parts of urea, 6 parts of ammonium dihydrogen phosphate, 8 parts of potassium sulfate 12 parts, 12 parts of humic acid, 8 parts of CaSO ₄ are mixed evenly, and after granulation, it is obtained.

Example 3

The first step, the hydrolysis of bean dregs: by weight, take 11 parts of bean dregs, after washing with water, add bean dregs to 550 parts of water, then add green wood enzyme, the amount of green wood enzyme added is 0.2% of the weight of bean dregs, and use 2wt% dilute hydrochloric acid to adjust the pH to 4-6, and carry out the hydrolysis reaction of cellulose. The hydrolysis reaction time is 18 hours, the reaction temperature is 42°C, and the enzyme is killed at a high temperature above 95°C;

In the second step, the hydrolyzate in the first step is concentrated under reduced pressure to 25% of its original volume, and then the bacterial liquid of Saccharomyces cerevisiae and Bacillus subtilis is added for fermentation treatment. The fermentation temperature is 32°C, the fermentation time is 60h, and then vacuum dried. , to obtain fermented bean dregs;

Step 3, the preparation of the water phase: by weight, 25 parts of fermented bean dregs, 450 parts of water, 3 parts of sodium octadecylbenzenesulfonate, 6 parts of monoglycerides, and 11 parts of methyl methacrylate , 18 parts of methacrylic acid, 4 parts of chitosan, 3 parts of N-isopropylacrylamide, 0.3 part of acetic acid, mixed evenly, and 2 parts of potassium persulfate to obtain the water phase;

Step 4, preparation of the oil phase: in parts by weight, mix toluene and chlorobenzene at a weight ratio of 1:1.1, then add SPAN-40 at 0.15% by weight of the mixture, and mix well to obtain the oil phase;

Step 5, reversed-phase suspension polymerization: After mixing the water phase and the oil phase according to the volume ratio of 1:5, nitrogen gas is introduced into the reactor, and then N,N,N',N'-tetramethylethylenediamine is added , reacting at 80°C under nitrogen for 3 hours; after the reaction, the solid matter was filtered out and washed with ethanol to obtain a granular slow-release fertilizer base material;

The 6th step, by weight, 45 parts of granular slow-release fertilizer base material, 22 parts of attapulgite, 5 parts of EM bacteria liquid are mixed uniformly, then with 3 parts of urea, 5 parts of ammonium dihydrogen phosphate, 6 parts of potassium sulfate 11 parts of humic acid and 6 parts of CaSO ₄ are mixed evenly, and after granulation, it is obtained.

Comparative example 1

The difference from Example 3 is that the bean dregs fermented product does not react with the granular slow-release fertilizer base material, but is directly mixed with other raw materials in step 6 according to the original weight ratio.

The first step, the hydrolysis of bean dregs: by weight, take 11 parts of bean dregs, after washing with water, add bean dregs to 550 parts of water, then add green wood enzyme, the amount of green wood enzyme added is 0.2% of the weight of bean dregs, and use 2wt% dilute hydrochloric acid to adjust the pH to 4-6, and carry out the hydrolysis reaction of cellulose. The hydrolysis reaction time is 18 hours, the reaction temperature is 42°C, and the enzyme is killed at a high temperature above 95°C;

In the second step, the hydrolyzate in the first step is concentrated under reduced pressure to 25% of its original volume, and then the bacterial liquid of Saccharomyces cerevisiae and Bacillus subtilis is added for fermentation treatment. The fermentation temperature is 32°C, the fermentation time is 60h, and then vacuum dried. , to obtain fermented bean dregs;

Step 3, the preparation of the water phase: by weight, 450 parts of water, 3 parts of sodium octadecylbenzenesulfonate, 6 parts of monoglycerides of fatty acids, 11 parts of methyl methacrylate, and 18 parts of methacrylic acid , 4 parts of chitosan, 3 parts of N-isopropylacrylamide, 0.3 part of acetic acid, and 2 parts of potassium persulfate are mixed uniformly to obtain the water phase;

Step 4, preparation of the oil phase: in parts by weight, mix toluene and chlorobenzene at a weight ratio of 1:1.1, then add SPAN-40 at 0.15% by weight of the mixture, and mix well to obtain the oil phase;

Step 5, reversed-phase suspension polymerization: After mixing the water phase and the oil phase according to the volume ratio of 1:5, nitrogen gas is introduced into the reactor, and then N,N,N',N'-tetramethylethylenediamine is added , reacting at 80°C under nitrogen for 3 hours; after the reaction, the solid matter was filtered out and washed with ethanol to obtain a granular slow-release fertilizer base material;

The 6th step, in parts by weight, mix 16.8 parts of bean dregs fermentation products, 28.2 parts of granular slow-release fertilizer base materials, 22 parts of attapulgite, and 5 parts of EM bacterial liquid, and then mix them with 3 parts of urea, ammonium dihydrogen phosphate 5 parts, 6 parts of potassium sulfate, 11 parts of humic acid, 6 parts of CaSO ₄ , mixed evenly, and granulated to obtain.

Comparative example 2

The difference with Example 3 is: chitosan is not added in the water phase in the reverse phase suspension polymerization.

The first step, the hydrolysis of bean dregs: by weight, take 11 parts of bean dregs, after washing with water, add bean dregs to 550 parts of water, then add green wood enzyme, the amount of green wood enzyme added is 0.2% of the weight of bean dregs, and use 2wt% dilute hydrochloric acid to adjust the pH to 4-6, and carry out the hydrolysis reaction of cellulose. The hydrolysis reaction time is 18 hours, the reaction temperature is 42°C, and the enzyme is killed at a high temperature above 95°C;

In the second step, the hydrolyzate in the first step is concentrated under reduced pressure to 25% of its original volume, and then the bacterial liquid of Saccharomyces cerevisiae and Bacillus subtilis is added for fermentation treatment. The fermentation temperature is 32°C, the fermentation time is 60h, and then vacuum dried. , to obtain fermented bean dregs;

Step 3, the preparation of the water phase: by weight, 25 parts of fermented bean dregs, 450 parts of water, 3 parts of sodium octadecylbenzenesulfonate, 6 parts of monoglycerides, and 11 parts of methyl methacrylate , 18 parts of methacrylic acid, 3 parts of N-isopropylacrylamide, 0.3 parts of acetic acid, mixed evenly, and 2 parts of potassium persulfate to obtain the water phase;

Step 4, preparation of the oil phase: in parts by weight, mix toluene and chlorobenzene at a weight ratio of 1:1.1, then add SPAN-40 at 0.15% by weight of the mixture, and mix well to obtain the oil phase;

Step 5, reversed-phase suspension polymerization: After mixing the water phase and the oil phase according to the volume ratio of 1:5, nitrogen gas is introduced into the reactor, and then N,N,N',N'-tetramethylethylenediamine is added , reacting at 80°C under nitrogen for 3 hours; after the reaction, the solid matter was filtered out and washed with ethanol to obtain a granular slow-release fertilizer base material;

The 6th step, by weight, 45 parts of granular slow-release fertilizer base material, 22 parts of attapulgite, 5 parts of EM bacteria liquid are mixed uniformly, then with 3 parts of urea, 5 parts of ammonium dihydrogen phosphate, 6 parts of potassium sulfate 11 parts of humic acid and 6 parts of CaSO ₄ are mixed evenly, and after granulation, it is obtained.

Comparative example 3

The difference from Example 3 is that the bean dregs have not been hydrolyzed.

Comparative example 4

The difference from Example 3 is that the bean dregs have not been fermented.

In the following experiments, the soil used is moderately saline-alkali coastal coastal flats in Jiangsu Province. The salt content of the 0-20cm plow layer soil in the test plot is 5.5-6.4g/kg, which belongs to moderate saline-alkali land, pH is 9.54, organic matter content is 8.45g/kg, total nitrogen is 0.43g/kg, and alkali-hydrolyzed nitrogen is 34.59mg /kg, available phosphorus is 9.21mg/kg, and available potassium is 191.87mg/kg. Determination methods of basic soil samples: organic matter: potassium dichromate volumetric method; total nitrogen: semi-micro Kelvin method; total phosphorus: sodium hydroxide fusion-molybdenum antimony scandium colorimetric method; alkaline nitrogen: alkaline solution diffusion method; available phosphorus : sodium bicarbonate extraction method; available potassium: ammonium acetate extraction flame photometry.

A total of 8 treatments were set up, and each treatment was repeated 3 times. There were 24 plots in total, and the plot area was 100 m ² . Except control, other each treatment is designed according to the principle of equal fertilization, is 120kg/mu, and fertilization position is planting ditch both sides, and fertilization is to dig deep 10cm wide 15cm ditch, cover soil after fertilization. Random arrangement was adopted, and various field managements were the same as local routines during the experiment. The test crop is maize, the variety is Dafeng 30, and the growth period is 127 days. Fertilization was applied on April 22, 2015, corn was sown on April 23, and soil and air samples were collected at the jointing stage, heading stage, and maturity stage of corn on June 21, August 2, and September 21 of the same year. The soil samples were the non-rhizosphere soil of corn, and the collection depth was 0-20 cm.

Determination of Changes in Total Nitrogen Content in Soil

After fertilization, the soil with a sampling depth of 0-20 cm was taken at 2-58 days and every 4 days to measure the total nitrogen content.

(1) Pretreatment of soil samples

1. Weigh three 0.5g soil samples into 25ml colorimetric tubes, add 10ml potassium persulfate solution, and shake well.

2. Put it in an oven and digest it at 125 degrees Celsius for an hour.

3. After the samples are cooled, transfer them to 500ml beakers and dilute to 500ml with ammonia-free water.

4. After normal pressure filtration, get the filtrate, adjust the pH to 7, measure two 2.50ml filtrates in 25ml colorimetric tubes, dilute to the mark, and use it as the sample to be tested.

(2) Preparation of standard series solutions

1. Preparation of standard solution: Measure 5.00ml of potassium nitrate standard stock solution in a 50ml colorimetric tube, and dilute to the mark to obtain a standard solution.

2. Preparation of standard series solutions: pipette 0.00, 0.50, 1.00, 2.00, 3.00, 5.00ml of standard solutions into 25ml colorimetric tubes, first dilute to 10ml, then add 5ml of alkaline potassium persulfate solution respectively.

3. Put it in an oven and digest it at 125 degrees Celsius for one hour.

4. After the solution is cooled, add 1ml hydrochloric acid solution respectively, shake well and wait for the test.

(3) Measure the absorbance of the solution and make a standard curve

1. Using a 10ml quartz cuvette, measure the absorbance of each solution at 220nm and 275nm wavelengths on a UV spectrophotometer with water as a reference, and shake the solution evenly before measuring the absorbance of the sample solution.

2. Corrected absorbance = -2 .

Result as shown in Figure 1, as can be seen from the figure, in comparative example 1 and 2, the release rate of total nitrogen content in the initial stage soil is faster, and the range of content fluctuation is, gradually descends from 34d, and adopts the example in the embodiment The release rate of total nitrogen is relatively uniform. It shows that the slow-release fertilizer prepared by the present invention has the effect of stabilizing the release rate.

Determination of soil respiration rate

Method: insert the base 5 cm into the soil between the corn rows, buckle the static box on the base and seal it with water. From 9:00 am to 11:00 am every day, gas is collected every 10 minutes from 0 min, for a total of 4 times. _CO gas samples were measured with an Agilent 7890B GC. The calculation formula of _CO2 emission flux (F) is:

;

In the formula: F is the CO ₂ emission flux/mg∙m ^-2 ∙h ^-1 ; ρ is the density of CO ₂ in the standard state/kg∙m ^-3 ; V and A are the volume and bottom area of the static tank, respectively, in units of are m ³ and m ² respectively; ∆c/∆t is the change rate of CO ₂ gas concentration in the static box within time ∆t; T is the temperature in the static box/°C.

^※ P<0.05 relative to blank group, *P<0.05 relative to Example 3 group

The main reason for soil respiration rate is that soil respiration is mainly produced by microbial oxidation of organic matter and root system respiration. As can be seen from the above table, the biological slow-release fertilizer used in the present invention can effectively make microorganisms act and continuously maintain plant root activity. , keeping the soil with a high respiration rate; in Comparative Example 1, because the bean dregs fermented product was not directly constructed on the interpenetrating network, the action efficiency of microorganisms was significantly lower than that of Example 3. The comparison chart is shown in Figure 2.

Determination of soil microbial biomass carbon

Take about 30 g of fresh soil samples, put them in a vacuum desiccator with a 50 mL alcohol-free chloroform beaker, sterilize them for 5 days, add 0.5 mol/L _K2SO4 to the sterilized soil samples _, Shake for 30 min and filter. A non-sterile control group was set up. Determination of soil microbial biomass carbon: absorb 15 mL of filtrate, add potassium dichromate solution to digest and measure, and the conversion factor is 0.4. Determination of soil microbial biomass nitrogen: absorb 15 mL of filtrate, add concentrated H ₂ SO ₄ , concentrate to about 3 mL after acidification, and then measure the total nitrogen in the filtrate according to the Kjeldahl method, and reduce nitrate nitrogen with Alloy , the conversion factor is 0.54.

^※ P<0.05 relative to blank group, *P<0.05 relative to Example 3 group

Soil microbial biomass is the source and sink of plant nutrients, the driving force for the transformation and circulation of soil organic matter and soil nutrients (N, P, K, etc.), participates in the decomposition of soil organic matter and the formation of humus, and is the source of soil carbon and nitrogen nutrients. Important parameters in transformation and circulation research. It can be seen from the above table that the soil microbial biomass carbon content of each fertilization treatment is higher than that of the control, and the difference is significant. The biological slow-release fertilizer adopted in the present invention can effectively promote the growth of microorganisms in the fertilizer, and improve the organic surface carbon and nitrogen content in the soil. Compared with the comparison example 3 groups, it can be seen that the bean dregs have been hydrolyzed, Fermentation treatment can effectively build it into the three-dimensional interpenetrating network of the slow-release fertilizer, making it easier for microorganisms to act on its surface and increasing the carbon content. The comparison chart is shown in Figure 3.

Determination of soil aggregates

According to Elliott soil aggregate wet sieving method (Elliott et.al,. Aggregate structures and carbon, nitrogen, and phosphorus in native aand cultivated soils [J]. SoilScience Society of America Journal, 1986, 50:627-633), here With slight changes on the basis, about 100g of air-dried soil samples were used to determine the composition of soil aggregates by institutional wet sieving method (machine model: ZY200-1, produced by Shanghai Dema Information Technology Co., Ltd.). The specific method is: place the sample on the sieve with the largest aperture, the apertures are 5mm, 2mm, 1mm, 0.5mm, 0.25mm from top to bottom, first soak in water for 10 minutes, when the whole set of sieves is at the bottom, the top sieve Keep the upper edge below the water surface, and vibrate vertically up and down for 10 minutes; transfer the soil particles from each level of sieve layer to aluminum boxes, remove moisture, dry and weigh them, and finally calculate the mass percentage of aggregates at each level. The stability of aggregates is expressed by the average weight diameter:

Among them, d _i represents the average particle diameter of soil aggregates of this grade, which is equal to the average value of the two-stage sieve holes in value, w _i represents the weight (g) of soil aggregate components of grade i, and W is soil aggregates of different particle sizes total weight.

^※ P<0.05 relative to blank group, *P<0.05 relative to Example 3 group

It can be seen from the table that for large particles in the soil (>5mm and 5-2mm grading), it is of great help to improve soil water permeability and air permeability. Water-based attapulgite and polyacrylate copolymer, its surface has stronger hydrophilicity, easily makes the aggregate in the soil increase by the mode of adsorption, improves the ratio of large particle; Through embodiment 3 and comparative example 2 can It can be seen that due to the addition of chitosan to the slow-release organic fertilizer, because it is a biodegradable natural raw material, it has good biocompatibility, rich surface groups, and good adsorption to metal ions, acids, etc. , after it is loaded through the polyacrylic resin network, it effectively promotes the adsorption of slow-release fertilizers and improves the classification of soil aggregates. It shows that the organic bacterial fertilizer provided by the invention can significantly increase the particle size water-stable aggregate content, improve the soil aggregate structure, and increase the stability and erosion resistance of the soil structure.

Determination of soil electrical conductivity

The soil contains salt ions, and the salt content is positively correlated with the conductivity. In this experiment, a conductivity meter was used to measure the conductivity of the soil leachate. The conductivity meter and conductivity thermometer produced by Shanghai Precision Scientific Instrument Co., Ltd. were used. Accurately weigh 5g of air-dried soil that has been sieved by 1mm, place it in a 100ml dry conical flask, add 25m1 of ultrapure water (soil-to-water ratio 1:5), shake for 10min, filter the supernatant into the conical flask with medium-speed filter paper Then read it with a conductivity meter.

^※ P<0.05 relative to blank group, *P<0.05 relative to Example 3 group

Soil conductivity is one of the most important factors in the soil. The higher the salt content in the soil, the greater the osmotic pressure of the leachate, and the greater the conductivity. Conductivity values are influenced both by external factors and by the intrinsic conditions of the soil. There is a strong relationship with the organic matter in the soil, microbial activity and the complex mineral composition of the soil. If the conductivity value in the soil is too high, the salt content will be high, which will inhibit the absorption of nutrients in the soil. It can be seen from the table that after the treatment with organic bacterial fertilizer, compared with the blank group, the application of microbial fertilizer in the soil can effectively promote the enhancement of microbial activity in the soil, and the microbial diversity reduces the conductivity value. Wherein, embodiment 3 can find out with respect to comparative example 2, because the grafting of chitosan on slow-release fertilizer base material can effectively make inorganic salt be adsorbed on its surface by the hydrophilicity of chitosan, adsorptivity, more It is easily metabolized by microorganisms and reduces the salt conductivity in the soil; at the same time, due to the ion exchange effect of calcium sulfate, it can effectively convert the sodium ions in the soil into sodium sulfate, which is taken away by rainwater.

Determination of soil salinity

Adopt the standard dry residue method, weigh 10g of air-dried soil sieved 1mm, place it in a 100ml dry Erlenmeyer flask, add 50ml of ultrapure water (soil-to-water ratio 1:5), vibrate for 10min, and filter the upper surface with medium-speed filter paper. Filter the clear liquid into a triangular flask, accurately draw 20ml of clear filtrate, put it into a known weighing bottle and evaporate it to dryness in a water bath, then add a little H ₂ O ₂ until the residue is white, take it out and put it in an oven for 2 hours , take it out to cool for 30 minutes, and weigh it on a balance.

Result calculation:

Soil total salt content (g/kg)=(W ₀ -W ₁ )×50×1000/(W×20)

In the formula: W ₁ is the weight of the weighing bottle (g), W ₀ is the weight of the weighing bottle + residue weight (g), and W is the dry weight of the sample (g).

^※ P<0.05 relative to blank group, *P<0.05 relative to Example 3 group

Excessive water-soluble salinity is an important feature of salinized soil, and high salinity inhibits plant growth. The accumulation of soluble salt NaCl on the soil surface is the most obvious characteristic of salinized soil. As can be seen from the table, the biological bacterial fertilizer of the present invention can effectively reduce the content of total inorganic salts in saline-alkali soil. Through the application of microbial fertilizer, the environmental characteristics of the soil are improved, the soil structure and firmness are improved, and the soil aggregates are increased, which is conducive to reducing the evaporation of water on the soil surface, thereby reducing the accumulation of salt ions on the surface and reducing the salt content. On the other hand, the planting of salt-tolerant plants is conducive to the absorption of salt in the soil surface.

Determination of Soil Water Soluble Ions Na ⁺ , K ⁺

The volumetric method was used to determine the water-soluble calcium and magnesium ions in soil. Weigh 20g of air-dried and sieved soil sample, place it in a 250ml Erlenmeyer flask, add 100ml of ultrapure water, shake for 10min, and then filter to obtain the test solution. Draw 10ml of the test solution into a test tube and measure it on an atomic absorption spectrophotometer.

Calculation results:

Potassium ion (K+) g/kg=C ₁ ×V/m;

Sodium ion (Na+) g/kg=C ₂ ×V/m;

In the formula, C ₁ is the potassium ion concentration (mg/L) measured by the working curve; C ₂ is the potassium ion concentration (mg/L) measured by the working curve; V is the volume of the leachate (mL); m is the drying Soil weight (g).

^※ P<0.05 relative to blank group, *P<0.05 relative to Example 3 group

Soil water-soluble salt ions are the most direct factor affecting the absorption of soil nutrients by plants in salinized soil. Excessively high salt ions will inhibit the normal growth of plants. Excessive sodium ions are harmful to plant growth and poisonous to plants. root system, and will deteriorate the physical properties of the soil. Because there is antagonism between sodium ions and potassium ions, high concentrations of sodium ions will inhibit the absorption of potassium, which is not conducive to the absorption of plant roots. As can be seen from the above table, the soil colloid in the microbial fertilizer can replace the sodium ion in the soil, so that the physical properties in the soil can be improved, so the surface layer sodium ion content gradually decreases; 4, due to the construction of proteins and peptides into the slow-release fertilizer in a three-dimensional space network, it effectively promotes the growth of microorganisms and improves the elimination rate of sodium ions; at the same time, with the decline of sodium ions, it inhibits the The activity of potassium ions will gradually increase, and the soluble ions in the soil will increase, which is conducive to the absorption of potassium by plants. The comparison chart is shown in Figure 4.

corn planting experiment

After planting, corn harvesting was carried out in each treatment field, and 100 grains were randomly selected in each group to count the total weight, and the thousand-grain weight was calculated; 10 corn plants were randomly selected in each test group, and the total plant yield, stem diameter and average height above the ground were counted, and calculated The average yield per plant, average stem diameter and average height above ground are shown in the table below:

^※ P<0.05 relative to blank group, *P<0.05 relative to Example 3 group

It can be seen from the above table that the growth of corn can be effectively promoted after using the above-mentioned organic bacterial fertilizer.

Claims (10)

1. a kind of slow-release bio bacterial manure, it is characterised in that it is bean dregs, the high molecular polymerization by being crossed by hydrolysis and fermentation process Thing, attapulgite, EM bacterium solutions, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, humic acid, CaSO₄It is prepared as raw material.

2. slow-release bio bacterial manure according to claim 1, it is characterised in that the nitrogenous fertilizer is urea.

3. slow-release bio bacterial manure according to claim 1, it is characterised in that the phosphate fertilizer is ammonium dihydrogen phosphate.

4. slow-release bio bacterial manure according to claim 1, it is characterised in that the potash fertilizer is potassium sulfate.

5. a kind of preparation method of slow-release bio bacterial manure, it is characterised in that include the following steps：

1st step, the hydrolysis of bean dregs：By weight, 10~12 parts of bean dregs are taken, after washing with water, bean dregs are added to 500~ In 600 parts of water, cellulase is added, and with dilute acid for adjusting pH to 4~6, carry out cellulose hydrolysis, then at enzyme deactivation Reason；

2nd step, after the hydrolysate of the 1st step is concentrated under reduced pressure, adds bacterium solution and carries out fermentation process, then after vacuum drying, obtain To fermenting bean dregs thing；

3rd step, the preparation of water phase：By weight, by 20~35 parts of fermenting bean dregs thing, 400~500 parts of water, anionic surface 1~4 part of activating agent, 4~8 parts of emulsifying agent, 10~12 parts of methyl methacrylate, 15~20 parts of methacrylic acid, chitosan 3~ 5 parts, 2~4 parts of n-isopropyl acrylamide, 0.2~0.5 part of acetic acid be uniformly mixed, 1~3 part of initiator, obtain water phase；

4th step, the preparation of oil phase：By weight, by toluene and chlorobenzene according to weight ratio 1：1~1.2 mixing, adds mixing The surfactant of thing weight 0.1~0.2%, after mixing, obtains oil phase；

5th step, inverse suspension polymerization：By water phase and oil phase according to volume ratio 1：After 3~6 mixing, nitrogen is passed through in the reactor, Add N, N, N ', N '-tetramethylethylenediamine, back flow reaction under a nitrogen atmosphere；After reaction, solids is filtered out, is used in combination Ethanol cleans, and obtains particulate slow-release fertilizer base-material；

6th step, by weight, by 40~50 parts of particulate slow-release fertilizer base-material, 20~25 parts of attapulgite, 4~8 parts of EM bacterium solutions Be uniformly mixed, then with 2~4 parts of urea, 4~6 parts of ammonium dihydrogen phosphate, 5~8 parts of potassium sulfate, 10~12 parts of humic acid, CaSO₄ 5~ 8 parts are uniformly mixed, and after granulation, to obtain the final product.

6. the preparation method of slow-release bio bacterial manure according to claim 5, it is characterised in that the diluted acid in the 1st step refers to 1 The dilute hydrochloric acid of~5wt%, the addition of cellulase are the 0.05~0.5% of bean dregs weight, and the cellulase is green wood Enzyme, hydrolysis time are 12~24h, and reaction temperature is 40~45 DEG C；Destroy the enzyme treatment refers to more than 95 DEG C high temperature enzyme deactivations.

7. the preparation method of slow-release bio bacterial manure according to claim 5, it is characterised in that the concentration in the 2nd step refers to Volume concentration is to original 20~30%, and fermentation temperature is 30~35 DEG C, and fermentation time is 40~80h；Bacterium solution refers to saccharomyces cerevisiae With the bacterium solution of bacillus subtilis.

8. the preparation method of slow-release bio bacterial manure according to claim 5, it is characterised in that described in the 3rd step it is cloudy from Sub- surfactant is selected from alkylbenzenesulfonate；Emulsifying agent in sucrose ester, soybean lecithin and mono fatty acid glyceride extremely Few one kind；Initiator is potassium peroxydisulfate.

9. the preparation method of slow-release bio bacterial manure according to claim 5, it is characterised in that the surface-active in the 4th step Agent is SPAN-40；Back flow reaction is 2~4h of reaction at 75~85 DEG C in 5th step.

10. application of the Claims 1 to 4 any one of them slow-release bio bacterial manure in salt affected soil fertilising.