CN108403756A - A kind of pet anti parasitic compound preparation - Google Patents
A kind of pet anti parasitic compound preparation Download PDFInfo
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- CN108403756A CN108403756A CN201810568158.7A CN201810568158A CN108403756A CN 108403756 A CN108403756 A CN 108403756A CN 201810568158 A CN201810568158 A CN 201810568158A CN 108403756 A CN108403756 A CN 108403756A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 52
- 150000001875 compounds Chemical class 0.000 title claims abstract description 43
- 230000002141 anti-parasite Effects 0.000 title claims abstract description 29
- 239000003096 antiparasitic agent Substances 0.000 title claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 239000000284 extract Substances 0.000 claims abstract description 31
- 241000246044 Sophora flavescens Species 0.000 claims abstract description 27
- 241001460390 Ganoderma lipsiense Species 0.000 claims abstract description 26
- 241000588724 Escherichia coli Species 0.000 claims abstract description 8
- 102000004447 HSP40 Heat-Shock Proteins Human genes 0.000 claims abstract description 8
- 108010042283 HSP40 Heat-Shock Proteins Proteins 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 30
- 239000000243 solution Substances 0.000 claims description 25
- 239000012141 concentrate Substances 0.000 claims description 24
- 238000000855 fermentation Methods 0.000 claims description 23
- 230000004151 fermentation Effects 0.000 claims description 23
- 239000002609 medium Substances 0.000 claims description 23
- 239000012752 auxiliary agent Substances 0.000 claims description 20
- 239000002532 enzyme inhibitor Substances 0.000 claims description 20
- 238000002156 mixing Methods 0.000 claims description 20
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
- 229940125532 enzyme inhibitor Drugs 0.000 claims description 14
- 238000007873 sieving Methods 0.000 claims description 14
- 239000002054 inoculum Substances 0.000 claims description 12
- 239000012460 protein solution Substances 0.000 claims description 12
- 238000011218 seed culture Methods 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- 229920002261 Corn starch Polymers 0.000 claims description 6
- 229930091371 Fructose Natural products 0.000 claims description 6
- 239000005715 Fructose Substances 0.000 claims description 6
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 235000019483 Peanut oil Nutrition 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000008120 corn starch Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000000312 peanut oil Substances 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 6
- 239000011691 vitamin B1 Substances 0.000 claims description 6
- 235000015099 wheat brans Nutrition 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 244000045947 parasite Species 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 9
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 5
- 230000002147 killing effect Effects 0.000 abstract description 5
- 230000000749 insecticidal effect Effects 0.000 abstract description 4
- ZSBXGIUJOOQZMP-BHPKHCPMSA-N sophoridine Chemical compound C1CC[C@@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-BHPKHCPMSA-N 0.000 abstract description 4
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 abstract description 3
- 239000000273 veterinary drug Substances 0.000 abstract description 3
- XVPBINOPNYFXID-JARXUMMXSA-N 85u4c366qs Chemical compound C([C@@H]1CCC[N@+]2(CCC[C@H]3[C@@H]21)[O-])N1[C@@H]3CCCC1=O XVPBINOPNYFXID-JARXUMMXSA-N 0.000 abstract description 2
- 102000002812 Heat-Shock Proteins Human genes 0.000 abstract description 2
- 108010004889 Heat-Shock Proteins Proteins 0.000 abstract description 2
- 102100034343 Integrase Human genes 0.000 abstract description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 abstract description 2
- 229930013930 alkaloid Natural products 0.000 abstract description 2
- 230000009137 competitive binding Effects 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 244000079386 endoparasite Species 0.000 abstract description 2
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 238000009472 formulation Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 210000000653 nervous system Anatomy 0.000 abstract description 2
- 229930015582 oxymatrine Natural products 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000011701 zinc Substances 0.000 abstract description 2
- 229910052725 zinc Inorganic materials 0.000 abstract description 2
- 102000003846 Carbonic anhydrases Human genes 0.000 abstract 2
- 108090000209 Carbonic anhydrases Proteins 0.000 abstract 2
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 abstract 1
- 230000001887 anti-feedant effect Effects 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 229930014456 matrine Natural products 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 7
- FSVJFNAIGNNGKK-UHFFFAOYSA-N 2-[cyclohexyl(oxo)methyl]-3,6,7,11b-tetrahydro-1H-pyrazino[2,1-a]isoquinolin-4-one Chemical compound C1C(C2=CC=CC=C2CC2)N2C(=O)CN1C(=O)C1CCCCC1 FSVJFNAIGNNGKK-UHFFFAOYSA-N 0.000 description 4
- 241000242722 Cestoda Species 0.000 description 4
- 241000244206 Nematoda Species 0.000 description 4
- 208000030852 Parasitic disease Diseases 0.000 description 4
- 239000000686 essence Substances 0.000 description 4
- 238000000643 oven drying Methods 0.000 description 4
- 229960002957 praziquantel Drugs 0.000 description 4
- -1 pyrazinoisoquinoline compound Chemical class 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 230000008020 evaporation Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 229960005134 pyrantel Drugs 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- AZSNMRSAGSSBNP-UHFFFAOYSA-N 22,23-dihydroavermectin B1a Natural products C1CC(C)C(C(C)CC)OC21OC(CC=C(C)C(OC1OC(C)C(OC3OC(C)C(O)C(OC)C3)C(OC)C1)C(C)C=CC=C1C3(C(C(=O)O4)C=C(C)C(O)C3OC1)O)CC4C2 AZSNMRSAGSSBNP-UHFFFAOYSA-N 0.000 description 2
- SPBDXSGPUHCETR-JFUDTMANSA-N 8883yp2r6d Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O[C@@H]([C@@H](C)CC4)C(C)C)O3)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1C[C@H](C)[C@@H]([C@@H](C)CC)O[C@@]21O[C@H](C\C=C(C)\[C@@H](O[C@@H]1O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C1)[C@@H](C)\C=C\C=C/1[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\1)O)C[C@H]4C2 SPBDXSGPUHCETR-JFUDTMANSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 229960002418 ivermectin Drugs 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 1
- 241000251556 Chordata Species 0.000 description 1
- 241000242711 Fasciola hepatica Species 0.000 description 1
- 241000935974 Paralichthys dentatus Species 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 229940125687 antiparasitic agent Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 244000078703 ectoparasite Species 0.000 description 1
- 208000006275 fascioliasis Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 229940041033 macrolides Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- YSAUAVHXTIETRK-AATRIKPKSA-N pyrantel Chemical compound CN1CCCN=C1\C=C\C1=CC=CS1 YSAUAVHXTIETRK-AATRIKPKSA-N 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 201000004409 schistosomiasis Diseases 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
- A61P33/12—Schistosomicides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of pet anti parasitic compound preparations, belong to veterinary drug preparation preparing technical field.The present invention is using ganoderma lipsiense as raw material, the extract with β carbonic anhydrase inhibitory activity is made, the enzymatic activity can effectively be inhibited, there is extensive killing effect to pet endoparasite, and on pet without influence, coordinate zinc fingers possessed by the heat shock protein in Escherichia coli DnaJ protein liquids, it can be with β carbonic anhydrase competitive bindings Zn2+Achieve the effect that Reverse transcriptase, improve the killing effect to parasite, a variety of alkaloids such as matrine, oxymatrine, the Sophoridine contained in cooperation shrubby sophora extract can be by the nervous system of destruction polypide, cause polypide Antifeedant Effects, play expelling parasite insecticidal effect, reach notable expelling parasite insecticidal effect, and is not likely to produce drug resistance.The present invention solve it is single for the current antiparasitic formulations product structure of animal in the market, to generate drug resistance, and to the poor problem of the control effect of parasite.
Description
Technical field
The invention belongs to veterinary drug preparation preparing technical field, more particularly to a kind of pet anti parasitic compound preparation.
Background technology
With the development of the social economy, people’s lives level is higher and higher, pet industry has also obtained unprecedented development, with
It pet and people’s lives is more and more closer, the prevention of pet parasitic disease is also increasingly valued by people.For a long time with
Come, anti parasitic class chemicals are almost relied on to the control of animal parasitosis.These drugs shortly after use,
Arthropod is just almost bar none to which create different degrees of drug resistance, and that there is also toxicity is big, long-term for chemicals
The disadvantages such as residual in livestock products and environment.Animal parasitosis is the main original for causing domestic and international animal husbandry huge economic losses
One of because.Currently, domestic ectoparasite disease mainly or relies on medical treatment, wherein chemicals are still leading at present anti-
Control means.In recent years, due to a variety of different type parasite mixing senses such as nematode, tapeworm, fluke and various vermins
Dye increases, and causes the therapeutic effect of folk prescription anti-parasite medicine undesirable.Ivermectin is semi-synthetic macrolides wide spectrum
Antiparasitic agent kills effect to internal epizoa especially nematode and vermin with good, but to tapeworm, suction
Worm and protozoan are invalid；Praziquantel is pyrazinoisoquinoline compound, which has special efficacy to snail fever, while also to tapeworm
Adult, larva it is effective, but praziquantel is invalid to nematode and vermin.Therefore, as can working out rational preparation work
Anti-parasitic compound preparation is made in drug by skill, and the pest-resistant spectrum of the two will be made to form perfect complementation, formed to nematode, tapeworm, suction
The effective anti-parasitic compound preparation of a variety of different type parasitic infections such as worm and various vermins.However,
China's veterinary drug clinic does not have ripe anti-parasitic compound preparation also, though developed countries have many compound anti-parasitic systems
Agent, such as ivermectin+Pyrantel compound preparations of Merial companies, praziquantel+Pyrantel+Fei Bantuo compounds of Bayer companies
Preparation, Mile companies praziquantel+Pyrantel compound preparation etc., but do not have ideal anti-parasitic compound preparation yet so far
Listing.
Invention content
The technical problems to be solved by the invention：For the current antiparasitic formulations product structure list of animal in the market
One, to generate drug resistance, and to the poor problem of the control effect of parasite, provide a kind of pet anti parasitic compound preparation.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of pet anti parasitic compound preparation includes following component according to the mass fraction：6 ~ 15 parts of essence, 4 ~ 10 portions of sucrose, 5 ~
10 parts of auxiliary agents, 20 ~ 40 parts of water, which is characterized in that further include 20 ~ 30 parts of shrubby sophora extracts, 30 ~ 50 parts of compound enzyme inhibitors；
The preparation method of the shrubby sophora extract：It takes kuh-seng to dry, pulverize sieving, takes sieving particle in mass ratio 1：4 ~ 6 are added
Methanol impregnates 2 ~ 3h, and in 50 ~ 60 DEG C of ultrasonic wave extractions, filtering takes filtrate, rotary evaporation, be concentrated under reduced pressure into original volume 20 ~
30%, concentrate is obtained, concentrate in mass ratio 1 is taken：3 ~ 4 are added acetic acid solution mixing, stir, and filtering takes filtrate, is concentrated under reduced pressure,
Medicinal extract is obtained, it is dry, obtain shrubby sophora extract；
The preparation method of the compound enzyme inhibitor, includes the following steps：
（1）Ganoderma lipsiense cleaning, drying are taken, are pulverized and sieved, sieving particle in mass ratio 1 is taken：2 ~ 3 are added water, obtain ganoderma lipsiense
0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5%, at 28 ~ 35 DEG C, enzyme is added in liquid
2 ~ 3h is solved, enzymolysis liquid is obtained；
（2）In 28 ~ 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, shaking table shaken cultivation 4 ~ 6 days obtains just
Culture solution takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 ~ 7 days, obtain zymotic fluid, zymotic fluid is taken to centrifuge,
It takes supernatant to be evaporated under reduced pressure to the 25 ~ 40% of original volume, obtains fermented concentrate；
（3）In mass ratio 1：5 ~ 8 take Escherichia coli DnaJ protein liquids, acetic acid solution mixing, stand, diluted protein solution are obtained, by matter
Measure ratio 2：1 takes diluted protein solution to be uniformly mixed with fermented concentrate to get compound enzyme inhibitor.
The auxiliary agent：In mass ratio 4：1 ~ 2, extracting corn starch, peanut oil mixing are to get auxiliary agent.
The step（2）In seed culture medium：According to the mass fraction, take 20 ~ 30 parts of wheat bran, 6 ~ 15 parts of glucose, 8 ~
16 parts of yeast extracts, 1000 parts of water, the 15 ~ 20min that sterilizes in 121 DEG C is to get fermentation medium.
The step（2）In fermentation medium：According to the mass fraction, 20 ~ 30 portions of potatos, 6 ~ 15 parts of fructose, 3 ~ 8 are taken
Part NaNO3, 4 ~ 8 parts（NH4)2SO4, 0.4 ~ 0.9 part of vitamin B1, 1000 parts of water mixing, sterilize in 121 DEG C 15 ~ 20min to get
Fermentation medium.
Compared with other methods, advantageous effects are the present invention：
The present invention is using ganoderma lipsiense as raw material, and through operations such as crushing, enzymolysis, fermented and cultureds, being made, there is β-carbonic anhydrase to inhibit
Active extract can effectively inhibit the enzymatic activity, and β-carbonic anhydrase is a kind of metalloenzyme, to pathogenic bacteria and parasite
Class metabolism is essential, is catalyzed CO2Aquation, participate in many physiology and pathologic process, including breathing, pH and CO2Stable state,
But without this enzyme in vertebrate and chordate animal body, therefore there can be extensive killing effect to pet endoparasite, and
On pet without influence, coordinate zinc fingers possessed by the heat shock protein in Escherichia coli DnaJ protein liquids, it can be with β-carbonic acid
Acid anhydride enzyme competitive binding Zn2+Achieve the effect that Reverse transcriptase, improve the killing effect to parasite, coordinates and contain in shrubby sophora extract
A variety of alkaloids such as some matrines, oxymatrine, Sophoridine can cause polypide food refusal by the nervous system of destruction polypide
Effect, plays expelling parasite insecticidal effect, reaches notable expelling parasite insecticidal effect, and be not likely to produce drug resistance.
Specific implementation mode
Auxiliary agent：In mass ratio 4：1 ~ 2, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 20 ~ 30 parts of wheat bran, 6 ~ 15 parts of glucose, 8 ~ 16 parts of yeast extracts, 1000 are taken
Part water, the 15 ~ 20min that sterilize in 121 DEG C are to get fermentation medium.
Fermentation medium：According to the mass fraction, 20 ~ 30 portions of potatos, 6 ~ 15 parts of fructose, 3 ~ 8 parts of NaNO are taken3, 4 ~ 8 parts
（NH4)2SO4, 0.4 ~ 0.9 part of vitamin B1, 1000 parts of water mixing, the 15 ~ 20min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 60 ~ 75 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Particle in mass ratio 1：4 ~ 6, which are added methanol, impregnates 2 ~ 3h, 40 ~ 60min of ultrasonic wave extraction, mistake under 50 ~ 60 DEG C, 300W power
Filter, takes filter residue, rotary evaporation to be concentrated under reduced pressure into the 20 ~ 30% of original volume, obtain concentrate, takes concentrate in mass ratio 1：3 ~ 4 add
Enter the acetic acid solution that mass fraction is 10% to mix, stir, filtering takes filtrate, is concentrated under reduced pressure, obtains medicinal extract, is done at 60 ~ 75 DEG C
It is dry to water content 5% hereinafter, shrubby sophora extract.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 60 ~ 75 DEG C of drying boxes, and took sieving particle in mass ratio 1：2~
3 are added water, obtain ganoderma lipsiense liquid, and 0.3% cellulose of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added
Enzyme digests 2 ~ 3h, obtains enzymolysis liquid at 28 ~ 35 DEG C；
（2）In 28 ~ 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, is vibrated with 160 ~ 230r/min shaking tables
Culture 4 ~ 6 days obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 ~ 7 days, must ferment
Liquid is taken zymotic fluid to be centrifuged 10 ~ 15 minutes with 4000 ~ 5000r/min, takes supernatant to be evaporated under reduced pressure to the 25 ~ 40% of original volume, obtain
Fermented concentrate；
（3）In mass ratio 1：5 ~ 8 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, 15 ~ 20 DEG C of perseverances
Temperature stands 30 ~ 50min, obtains diluted protein solution, in mass ratio 2：1 take diluted protein solution to be uniformly mixed with fermented concentrate to get
Compound enzyme inhibitor.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 30 ~ 50 parts of compound enzyme inhibitors, 20 ~ 30 parts of shrubby sophora extracts, 6 ~ 15 parts of essence, 4 ~ 10 parts
Sucrose, 5 ~ 10 parts of auxiliary agents, 20 ~ 40 parts of water；
（2）In 40 ~ 65 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, is stirred with 250 ~ 300r/min
30 ~ 50min is cooled to 20 ~ 25 DEG C, and complex enzyme inhibitor mixed is added, and ultrasonic wave disperses 10 ~ 15min to get the anti-parasitism of pet
Worm compound preparation.
Auxiliary agent：In mass ratio 4：1, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 20 parts of wheat bran, 6 parts of glucose, 8 parts of yeast extracts, 1000 parts of water are taken, in 121
DEG C sterilizing 15min to get fermentation medium.
Fermentation medium：According to the mass fraction, 20 portions of potatos, 6 parts of fructose, 3 parts of NaNO are taken3, 4 parts（NH4)2SO4、0.4
Part vitamin B1, 1000 parts of water mixing, the 15min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 60 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Grain in mass ratio 1：4, which are added methanol, impregnates 2h, the ultrasonic wave extraction 40min under 50 DEG C, 300W power, and filtering takes filter residue, rotates
Evaporation, is concentrated under reduced pressure into the 20% of original volume, obtains concentrate, take concentrate in mass ratio 1：3 are added the second that mass fraction is 10%
Acid solution mixing, is stirred, and filtering takes filtrate, is concentrated under reduced pressure, obtains medicinal extract, is dried at 60 DEG C to water content 5% hereinafter, obtaining bitter
Conopsea extraction.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 60 DEG C of drying boxes, and took sieving particle in mass ratio 1：2 are added
Water obtains ganoderma lipsiense liquid, and 0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added, in
At 28 DEG C, 2h is digested, enzymolysis liquid is obtained；
（2）In 28 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, with 160r/min shaking tables shaken cultivation 4
It, obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 days, obtain zymotic fluid, take zymotic fluid
It is centrifuged 10 minutes with 4000r/min, takes supernatant to be evaporated under reduced pressure to the 25% of original volume, obtain fermented concentrate；
（3）In mass ratio 1：5 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, and 15 DEG C of constant temperature are quiet
30min is set, diluted protein solution, in mass ratio 2 are obtained：1 takes diluted protein solution to be uniformly mixed with fermented concentrate presses down to get complex enzyme
Preparation.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 30 parts of compound enzyme inhibitors, 20 parts of shrubby sophora extracts, 6 parts of essence, 4 portions of sucrose, 5 parts help
Agent, 20 parts of water；
（2）In 40 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, 30min, drop are stirred with 250r/min
Complex enzyme inhibitor mixed is added to 20 DEG C in temperature, and ultrasonic wave disperses 10min to get pet anti parasitic compound preparation.
Auxiliary agent：In mass ratio 4：1.5, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 25 parts of wheat bran, 10 parts of glucose, 12 parts of yeast extracts, 1000 parts of water are taken, in
121 DEG C of sterilizing 18min are to get fermentation medium.
Fermentation medium：According to the mass fraction, 25 portions of potatos, 10 parts of fructose, 5 parts of NaNO are taken3, 6 parts（NH4)2SO4、
0.6 part of vitamin B1, 1000 parts of water mixing, the 18min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 68 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Grain in mass ratio 1：5, which are added methanol, impregnates 2.5h, the ultrasonic wave extraction 50min under 55 DEG C, 300W power, and filtering takes filter residue, revolves
Turn evaporation, is concentrated under reduced pressure into the 25% of original volume, obtains concentrate, take concentrate in mass ratio 1：3.5 addition mass fractions are 10%
Acetic acid solution mixing, stir, filtering, take filtrate, be concentrated under reduced pressure, obtain medicinal extract, at 68 DEG C dry to water content 5% hereinafter,
Obtain shrubby sophora extract.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 68 DEG C of drying boxes, and took sieving particle in mass ratio 1：2.5 plus
Enter water, obtain ganoderma lipsiense liquid, 0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added,
At 32 DEG C, 2.5h is digested, enzymolysis liquid is obtained；
（2）In 32 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, with 200r/min shaking tables shaken cultivation 5
It, obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 6 days, obtain zymotic fluid, take zymotic fluid
It is centrifuged 13 minutes with 4500r/min, takes supernatant to be evaporated under reduced pressure to the 32% of original volume, obtain fermented concentrate；
（3）In mass ratio 1：7 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, and 18 DEG C of constant temperature are quiet
40min is set, diluted protein solution, in mass ratio 2 are obtained：1 takes diluted protein solution to be uniformly mixed with fermented concentrate presses down to get complex enzyme
Preparation.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 40 parts of compound enzyme inhibitors, 25 parts of shrubby sophora extracts, 10 parts of essence, 7 portions of sucrose, 8 parts help
Agent, 30 parts of water；
（2）In 55 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, 40min, drop are stirred with 280r/min
Complex enzyme inhibitor mixed is added to 23 DEG C in temperature, and ultrasonic wave disperses 13min to get pet anti parasitic compound preparation.
Auxiliary agent：In mass ratio 4：2, extracting corn starch, peanut oil mixing are to get auxiliary agent.
Seed culture medium：According to the mass fraction, 30 parts of wheat bran, 15 parts of glucose, 16 parts of yeast extracts, 1000 parts of water are taken, in
121 DEG C of sterilizing 20min are to get fermentation medium.
Fermentation medium：According to the mass fraction, 30 portions of potatos, 15 parts of fructose, 8 parts of NaNO are taken3, 8 parts（NH4)2SO4、
0.9 part of vitamin B1, 1000 parts of water mixing, the 20min that sterilize in 121 DEG C are to get fermentation medium.
The preparation method of shrubby sophora extract：Sophora flavescens cleaning is taken, in 75 DEG C of oven dryings, 80 mesh sieve is crushed, takes sieving
Grain in mass ratio 1：6, which are added methanol, impregnates 3h, the ultrasonic wave extraction 60min under 60 DEG C, 300W power, and filtering takes filter residue, rotates
Evaporation, is concentrated under reduced pressure into the 30% of original volume, obtains concentrate, take concentrate in mass ratio 1：4 are added the second that mass fraction is 10%
Acid solution mixing, is stirred, and filtering takes filtrate, is concentrated under reduced pressure, obtains medicinal extract, is dried at 75 DEG C to water content 5% hereinafter, obtaining bitter
Conopsea extraction.
The preparation method of compound enzyme inhibitor, includes the following steps：
（1）It takes ganoderma lipsiense to clean, dry, pulverize 60 mesh sieve in 75 DEG C of drying boxes, and took sieving particle in mass ratio 1：3 are added
Water obtains ganoderma lipsiense liquid, and 0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5% is added, in
At 35 DEG C, 3h is digested, enzymolysis liquid is obtained；
（2）In 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, with 230r/min shaking tables shaken cultivation 6
It, obtains just culture solution, takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 7 days, obtain zymotic fluid, take zymotic fluid
It is centrifuged 15 minutes with 5000r/min, takes supernatant to be evaporated under reduced pressure to the 40% of original volume, obtain fermented concentrate；
（3）In mass ratio 1：8 take Escherichia coli DnaJ protein liquids, the acetic acid solution that mass fraction is 8% mixes, and 20 DEG C of constant temperature are quiet
50min is set, diluted protein solution, in mass ratio 2 are obtained：1 takes diluted protein solution to be uniformly mixed with fermented concentrate presses down to get complex enzyme
Preparation.
A kind of preparation method of pet anti parasitic compound preparation, includes the following steps：
（1）According to the mass fraction, take 50 parts of compound enzyme inhibitors, 30 parts of shrubby sophora extracts, 15 parts of essence, 10 portions of sucrose, 10 parts
Auxiliary agent, 40 parts of water；
（2）In 65 DEG C of water-bath, shrubby sophora extract, essence, auxiliary agent, water are taken, is mixed in container, 50min, drop are stirred with 300r/min
Complex enzyme inhibitor mixed is added to 25 DEG C in temperature, and ultrasonic wave disperses 15min to get pet anti parasitic compound preparation.
Comparative example 1：It is essentially identical with the preparation method of embodiment 3, it has only the difference is that lacking shrubby sophora extract.
Comparative example 2：It is essentially identical with the preparation method of embodiment 3, it has only the difference is that lacking compound enzyme inhibitor.
Comparative example 3：The anti parasitic compound preparation of Yibin City company production.
Anti parasitic compound preparation prepared by embodiment 1,2,3 and comparative example 1,2,3,4 is respectively to suffering from liver rot, mite
The pet dog of worm is tested.Its test result is recorded in table 1.
Table 1：
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Kill shortest time/day of liver fluke | 17 | 15 | 13 | 30 | 28 | 25 |
| The shortest time of mite killing/day | 0.6 | 1 | 0.8 | 7 | 6 | 9 |
| Control effect/% | 97 | 95 | 98 | 72 | 69 | 80 |
In conclusion as shown in Table 1, anti parasitic compound preparation of the invention is to dynamic product structure comparatively perfect, and to parasite
Control effect it is preferable, have preferable development prospect.
Claims (4)
1. a kind of pet anti parasitic compound preparation includes following component according to the mass fraction：6 ~ 15 parts of essence, 4 ~ 10 parts of sucrose, 5
~ 10 parts of auxiliary agents, 20 ~ 40 parts of water, which is characterized in that further include 20 ~ 30 parts of shrubby sophora extracts, 30 ~ 50 parts of compound enzyme inhibitors；
The preparation method of the shrubby sophora extract：It takes kuh-seng to dry, pulverize sieving, takes sieving particle in mass ratio 1：4 ~ 6 are added
Methanol impregnates 2 ~ 3h, and in 50 ~ 60 DEG C of ultrasonic wave extractions, filtering takes filtrate, rotary evaporation, be concentrated under reduced pressure into original volume 20 ~
30%, concentrate is obtained, concentrate in mass ratio 1 is taken：3 ~ 4 are added acetic acid solution mixing, stir, and filtering takes filtrate, is concentrated under reduced pressure,
Medicinal extract is obtained, it is dry, obtain shrubby sophora extract；
The preparation method of the compound enzyme inhibitor, includes the following steps：
（1）Ganoderma lipsiense cleaning, drying are taken, are pulverized and sieved, sieving particle in mass ratio 1 is taken：2 ~ 3 are added water, obtain ganoderma lipsiense
0.3% cellulase of pectase and ganoderma lipsiense liquid quality of ganoderma lipsiense liquid quality 0.5%, at 28 ~ 35 DEG C, enzyme is added in liquid
2 ~ 3h is solved, enzymolysis liquid is obtained；
（2）In 28 ~ 35 DEG C, enzymolysis liquid is taken to be seeded to seed culture medium by 10% inoculum concentration, shaking table shaken cultivation 4 ~ 6 days obtains just
Culture solution takes first culture solution to be seeded to fermentation medium by 10% inoculum concentration, cultivates 5 ~ 7 days, obtain zymotic fluid, zymotic fluid is taken to centrifuge,
It takes supernatant to be evaporated under reduced pressure to the 25 ~ 40% of original volume, obtains fermented concentrate；
（3）In mass ratio 1：5 ~ 8 take Escherichia coli DnaJ protein liquids, acetic acid solution mixing, stand, diluted protein solution are obtained, by matter
Measure ratio 2：1 takes diluted protein solution to be uniformly mixed with fermented concentrate to get compound enzyme inhibitor.
2. pet anti parasitic compound preparation according to claim 1, which is characterized in that the auxiliary agent：In mass ratio 4：1 ~ 2,
Extracting corn starch, peanut oil mixing are to get auxiliary agent.
3. pet anti parasitic compound preparation according to claim 1, which is characterized in that the step（2）In seed culture
Base：According to the mass fraction, 20 ~ 30 parts of wheat bran, 6 ~ 15 parts of glucose, 8 ~ 16 parts of yeast extracts, 1000 parts of water are taken, in 121 DEG C of sterilizings
15 ~ 20min is to get fermentation medium.
4. pet anti parasitic compound preparation according to claim 1, which is characterized in that the step（2）In fermented and cultured
Base：According to the mass fraction, 20 ~ 30 portions of potatos, 6 ~ 15 parts of fructose, 3 ~ 8 parts of NaNO are taken3, 4 ~ 8 parts（NH4)2SO4, 0.4 ~ 0.9 part
Vitamin B1, 1000 parts of water mixing, the 15 ~ 20min that sterilize in 121 DEG C are to get fermentation medium.
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| CN201810568158.7A CN108403756A (en) | 2018-06-05 | 2018-06-05 | A kind of pet anti parasitic compound preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015598A (en) * | 2007-02-14 | 2007-08-15 | 王东辉 | Externally used pharmaceutical preparation for treating disease caused by acarids |
| CN101389764A (en) * | 2005-08-01 | 2009-03-18 | Abr有限公司 | Sugar composition of basidiomycete fungi as pesticide against pathogens |
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| CN104069217A (en) * | 2014-07-18 | 2014-10-01 | 青岛科维源环保技术有限公司 | Mongolian medicine for pet dog anti-parasitism and preparation method of anaesthetic preparation |
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2018
- 2018-06-05 CN CN201810568158.7A patent/CN108403756A/en not_active Withdrawn
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|---|---|---|---|---|
| CN101389764A (en) * | 2005-08-01 | 2009-03-18 | Abr有限公司 | Sugar composition of basidiomycete fungi as pesticide against pathogens |
| CN101015598A (en) * | 2007-02-14 | 2007-08-15 | 王东辉 | Externally used pharmaceutical preparation for treating disease caused by acarids |
| CN102028644A (en) * | 2009-09-30 | 2011-04-27 | 湖北汇特生物医药技术有限公司 | Toiletries based on the medicinal properties of related extracts from mugwort |
| CN104069217A (en) * | 2014-07-18 | 2014-10-01 | 青岛科维源环保技术有限公司 | Mongolian medicine for pet dog anti-parasitism and preparation method of anaesthetic preparation |
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Application publication date: 20180817 |