CN108641946A - Bioreactor and the method that microorganism is cultivated based on bioreactor - Google Patents
Bioreactor and the method that microorganism is cultivated based on bioreactor Download PDFInfo
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- CN108641946A CN108641946A CN201810492502.9A CN201810492502A CN108641946A CN 108641946 A CN108641946 A CN 108641946A CN 201810492502 A CN201810492502 A CN 201810492502A CN 108641946 A CN108641946 A CN 108641946A
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Abstract
The invention belongs to field of microbial culture technology, and in particular to a kind of bioreactor and the method based on bioreactor cultivation microorganism, including cultivation collecting unit, air supply unit, culture medium feed unit and energy and device controller；The cultivation collecting unit includes multiple breeding devices for passing sequentially through circulation pipeline and being connected, and the circulation pipeline between two breeding devices is equipped with circulation solenoid valve and water pump；It is equipped with light compensating apparatus on each breeding device；Various sensors in each breeding device；The controller is equipped with for input information and shows the touch screen of information；The light compensating apparatus, various sensors, air supply unit, culture medium feed unit are connect with controller respectively.The present invention controls the growing environment of microorganism by the various data informations of the microorganism growing environment of acquisition, to create optimum growing environment for microorganism, improves the efficiency of micro-organisms.
Description
Technical field
The invention belongs to field of microbial culture technology, and in particular to a kind of bioreactor and be based on optical-biological reaction
The method that device cultivates microorganism.
Background technology
Microorganism can utilize simple mineral nutrient, be closed in the case of photosynthesis (daylight and artificial light)
At organic compound and it is proliferated.Since microorganism has efficient light utilization efficiency, and rapidly and efficiently inhaled from ambient enviroment
It receives nutrient (such as CO2, nitrogen, phosphorus) and is converted to the ability of organic compound, so that microorganism is had and solve food shortages, the energy
The tremendous economic potentiality of crisis and CO2 emission reductions.
Microorganism is cultivated generally in raceway pond, in glass pipe.It is cultivated simply in raceway pond, but is easy to be mixed into micro- life
The natural enemy of object and various miscellaneous bacterias etc., it is not easy to reach higher biological density, low output.Cultivated in glass pipe, equipment at
This is high, while the cultivation land used of unit area the case where there is also low yields.
Invention content
For the defects in the prior art, it is supported the present invention provides a kind of bioreactor and based on bioreactor
The method for growing microorganism controls the growing environment of microorganism by the various data informations of the microorganism growing environment of acquisition, from
And be that microorganism creates optimum growing environment, improve the efficiency of micro-organisms.
In a first aspect, the present invention provides a kind of bioreactor, including cultivation collecting unit, air supply unit, culture
Base feed unit and the controller that can be communicated with external equipment；
The cultivation collecting unit includes multiple breeding devices for passing sequentially through circulation pipeline and being connected, the stream between two breeding devices
Tube road is equipped with circulation solenoid valve and water pump；It is equipped with light compensating apparatus on each breeding device；It is equipped with illumination in each breeding device
Sensor, pH value sensor, dissolved oxygen sensor, temperature sensor, liquid level sensor and biosensor；
The air supply unit includes aeration tank, multiple oxygen channels, carbon dioxide tank and multiple carbon dioxide conduits, each
Oxygen channel connects aeration tank and a breeding device, and each oxygen channel is equipped with oxygen solenoid valve, each carbon dioxide conduit
Carbon dioxide tank and a breeding device are connected, each carbon dioxide conduit is equipped with carbon dioxide solenoid valve；
The culture medium feed unit includes culture tank and multiple culture medium pipelines, each culture medium pipeline connection culture
Base tank and a breeding device, each culture medium pipeline is equipped with culture medium solenoid valve and culture medium pumps；
The controller is equipped with for input information and shows the touch screen of information；
The light compensating apparatus, optical sensor, pH value sensor, dissolved oxygen sensor, temperature sensor, liquid level sensor,
Biosensor, water pump, oxygen solenoid valve, carbon dioxide solenoid valve, culture medium solenoid valve, culture medium pump, touches circulation solenoid valve
Control screen is electrically connected with the controller respectively.
Preferably, the breeding device lower part is equipped with the oxygen inlet of connection gas pipeline, connects the two of carbon dioxide conduit
The liquid outlet of carbonoxide import and connection flow tube road, the breeding device top be equipped with connection culture medium pipeline culture medium mouth,
The inlet in connection flow tube road and the exhaust outlet for exhaust.
Preferably, the light compensating apparatus includes outer light compensating apparatus and interior light compensating apparatus, and the side of the breeding device is uniformly set
There are vertical multiple outer light compensating apparatus, the location mid-shaft of the breeding device is equipped with the accommodating cavity of upper end opening, in the accommodating cavity
Equipped with vertical interior light compensating apparatus.
Preferably, the outer light compensating apparatus includes outer light filling holder and the LED light bar that is connect with outer light filling holder；
The outer light filling holder includes top plate and bottom plate and the side plate that is connected between top plate and bottom plate, and the side plate is in
The cylinder of evagination, and the inner surface of the side plate is provided with reflective layer, the both ends of the LED light bar are separately fixed at top plate and bottom
On plate.
Preferably, the interior light compensating apparatus includes cylindrical interior light filling holder and the LED light that is connect with interior light filling holder
Item；
The interior light filling holder includes upper plate, the cycle side plate of lower plate and connection between the upper and lower plates, the LED
The both ends of lamp bar are separately fixed at the center of upper plate and lower plate.
Preferably, the breeding device include the first breeding device ... N breeding devices, the first breeding device to N breeding devices, and
The volume of latter breeding device is more than the volume of previous breeding device, and the microorganism cultivation of different growing stage is housed in each breeding device
Liquid.
Second aspect, the present invention provides a kind of methods cultivating microorganism based on bioreactor, are suitable for first
Bioreactor described in aspect, includes the following steps：
S1 calculates the total volume after mixed culture medium according to the microbial species amount being initially added in breeding device, according to totality
Product control culture medium feed unit conveys culture medium into this breeding device；
S2 obtains the microbial biomass of the environmental data and biosensor acquisition of each sensor acquisition in this breeding device；Root
Room temperature is adjusted according to environmental data control light compensating apparatus light filling, control air supply unit gas supply and control room conditioning；Work as microorganism
When amount reaches given threshold, by control system automatic work, culturing liquid is transported in next breeding device and continues to cultivate, and is controlled
Culture medium feed unit processed conveys culture medium to next breeding device；Until microorganism is cultivated ripe.
Preferably, the breeding device include the first breeding device ... N breeding devices, the first breeding device to N breeding devices, and
The volume of latter breeding device is more than the volume of previous breeding device；
The microorganism cultivation liquid of different growing stage is housed, the culturing liquid of different growing stage has in each breeding device
Different optical density threshold ranges.
Preferably, the S1 is specially：
The microbial species amount for being initially put into the microorganism in breeding device is obtained by touch screen；
The culturing liquid total volume after mixed culture medium is calculated according to microbial species amount, calculating this according to culturing liquid total volume supports
Grow the level threshold in device；
The corresponding culture medium solenoid valve opening of this breeding device and culture medium pump work are controlled, the culture medium in culture tank is defeated
It is sent in this breeding device, when the level value of the corresponding liquid level sensor acquisition of this breeding device reaches level threshold, control culture
Base solenoid valve is closed and culture medium pump is stopped, and the culturing liquid after mixed culture medium starts to cultivate microorganism.
Preferably, the S2 is specially：
Optical sensor acquires the OD value of culturing liquid in this breeding device, when OD value is less than optical density threshold range
When, the corresponding light compensating apparatus work of this breeding device is controlled, light filling is carried out to culturing liquid, when OD value is higher than optical density threshold value model
When enclosing, control light compensating apparatus is stopped, and stops carrying out light filling to culturing liquid；
Dissolved oxygen sensor acquires the oxygen dissolved of culturing liquid in this breeding device, when oxygen dissolved is less than oxygen threshold range, control
The corresponding oxygen solenoid valve of this breeding device is opened, and the oxygen in aeration tank is transported in this breeding device, when oxygen dissolved is less than oxygen threshold
When being worth range, control oxygen solenoid valve is closed, and stops conveying oxygen；
PH sensor acquires the pH value of culturing liquid in this breeding device, when pH value is higher than PH threshold ranges, controls this cultivation
The corresponding carbon dioxide solenoid valve of device is opened, and the carbon dioxide in carbon dioxide tank is transported in this breeding device, when pH value is less than
When PH threshold ranges, control carbon dioxide solenoid valve is closed, and transport of carbon dioxide is stopped；
Temperature sensor acquires the liquid temperature value of culturing liquid in this breeding device, when liquid temperature value is below or above liquid temperature threshold range
When, control room conditioning adjusts room temperature, and then controls liquid temperature；
Biosensor acquires the microbial biomass of culturing liquid in this breeding device, when microbial biomass is more than concentration threshold, control
The corresponding circulation solenoid valve opening of this breeding device and pump working are made, the culturing liquid in this breeding device is transported to next breeding device
It is interior, and this stage breeding liquid total volume is calculated according to microbial biomass, and culture tank is controlled according to this stage breeding liquid total volume
Culture medium is conveyed to next breeding device, continues microorganism and is cultivated；
When microbial biomass reaches ripe threshold value, then microculture is ripe.
Beneficial effects of the present invention are：By the various data informations of the microorganism growing environment of acquisition, microorganism is controlled
Growing environment improve the efficiency of micro-organisms to create optimum growing environment for microorganism.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described.In all the appended drawings, similar element
Or part is generally identified by similar reference numeral.In attached drawing, each element or part might not be drawn according to actual ratio.
Fig. 1 is the structure chart of bioreactor in the present embodiment；
Fig. 2 is the overlooking structure figure of breeding device in the present embodiment；
Fig. 3 is the structure chart of the present embodiment China and foreign countries light compensating apparatus；
Fig. 4 is the structure chart of interior light compensating apparatus in the present embodiment；
Fig. 5 is the method flow diagram for cultivating microorganism in the present embodiment based on bioreactor.
Reference numeral：
1- breeding devices, 2- circulations solenoid valve, 3- water pumps, 4- light compensating apparatus, 5- optical sensors, 6-PH values sensor, 7-
Dissolved oxygen sensor, 8- temperature sensors, 9- liquid level sensors, 10- biosensors, 11- aeration tanks, 12- oxygen channels, 13-
Oxygen solenoid valve, 14- carbon dioxide tanks, 15- carbon dioxide conduits, 16- carbon dioxide solenoid valves, 17- culture tanks, 18- trainings
Support base tube road, 19- culture mediums solenoid valve, 20- culture mediums pump, 21- oxygen inlets, 22- carbon dioxide inlets, 23- liquid outlets,
24- culture mediums mouth, 25- inlets, 26- exhaust outlets, 27- accommodating cavities
The first breeding devices of 111-, the second breeding devices of 112-, 113- third breeding devices
Light compensating apparatus in the outer light compensating apparatus of 41-, 42-
411- top plates, 412- bottom plates, 413- side plates, 414-LED lamp bars
421- upper plates, 422- lower plates, 423- cycle side plates, 424-LED lamp bars
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are some of the embodiments of the present invention, instead of all the embodiments.Based on this hair
Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
It should be appreciated that ought use in this specification and in the appended claims, term " comprising " and "comprising" instruction
Described feature, entirety, step, operation, the presence of element and/or component, but one or more of the other feature, whole is not precluded
Body, step, operation, element, component and/or its presence or addition gathered.
It is also understood that the term used in this description of the invention is merely for the sake of the mesh for describing specific embodiment
And be not intended to limit the present invention.As description of the invention and it is used in the attached claims, unless on
Other situations are hereafter clearly indicated, otherwise " one " of singulative, "one" and "the" are intended to include plural form.
Embodiment one
A kind of bioreactor is present embodiments provided, for the culture to haematococcus pluvialis, as shown in Figure 1, including
Cultivate collecting unit, air supply unit, culture medium feed unit and the controller that can be communicated with external equipment；
The cultivation collecting unit includes three and passes sequentially through the connected breeding device 1 of circulation pipeline, between two breeding devices 1
The pipeline that circulates is equipped with circulation solenoid valve 2 and water pump 3；Three breeding devices 1 include the first breeding device 111,112 and of the second breeding device
Third breeding device 113, the interior microorganism cultivation liquid that different growing stage is housed of each breeding device 1, because with the life of microorganism
Long, the microbial biomass in later stage is more than the microbial biomass of early period, needs growing space and the culture medium of bigger, therefore latter breeding device
1 volume is more than the volume of previous breeding device 1, and three breeding devices 1 correspond to three growth periods of microorganism：Inoculation growth period,
Fast growing period and induction period can also be divided into other several periods in practical applications.
As shown in Fig. 2, light compensating apparatus 4 is equipped on each breeding device 1, in order to allow the culturing liquid in breeding device 1 to be filled
The illumination divided, therefore the light compensating apparatus 4 includes outer light compensating apparatus 41 and interior light compensating apparatus 42.It is described in order to allow uniform illumination
The side of breeding device 1 is uniformly provided with vertical multiple outer light compensating apparatus 41, and the location mid-shaft of the breeding device 1 is equipped with upper end opening
Accommodating cavity 27, vertical interior light compensating apparatus 42 is equipped in the accommodating cavity 27.
Wherein, as shown in figure 3, the outer light compensating apparatus 41 includes outer light filling holder and the LED being connect with outer light filling holder
Lamp bar 414；The outer light filling holder includes top plate 411, bottom plate 412 and the side being connected between top plate 411 and bottom plate 412
Plate 413, the side plate 413 is in the cylinder of evagination, and the inner surface of the side plate 413 is provided with reflective layer, the LED light bar
414 both ends are separately fixed on top plate 411 and bottom plate 412.
Wherein, as shown in figure 4, the interior light compensating apparatus 42 includes the interior light filling holder of cylinder and connects with interior light filling holder
The LED light bar 414 connect；The interior light filling holder include upper plate 421, lower plate 422 and be connected to upper plate 421 and lower plate 422 it
Between cycle side plate 423, the both ends of the LED light bar 424 are separately fixed at the center of upper plate 421 and lower plate 422.
By above-mentioned outer light compensating apparatus 41 and interior light compensating apparatus 42 in the present embodiment, no light dead angle allows culturing liquid to obtain
To abundant and uniform illumination.
It is equipped with optical sensor 5, pH value sensor 6, dissolved oxygen sensor 7, temperature sensor 8, liquid level in each breeding device 1
Sensor 9 and biosensor 10.
Each 1 lower part of breeding device is equipped with the oxygen inlet 21 of connection gas pipeline, connects the dioxy of carbon dioxide conduit 15
Change the liquid outlet 23 in carbon import 22 and connection flow tube road, 1 top of the breeding device is equipped with the culture of connection culture medium pipeline 18
Base mouth 24, the inlet 25 in connection flow tube road and the exhaust outlet 26 for exhaust.
The air supply unit includes 11, three oxygen channels 12 of aeration tank, carbon dioxide tank 14 and three carbon dioxide pipes
Road 15, each oxygen channel 12 connect aeration tank 11 and a breeding device 1, and each oxygen channel 12 is equipped with oxygen solenoid valve
13, each carbon dioxide conduit 15 connects carbon dioxide tank 14 and a breeding device 1, and each carbon dioxide conduit 15 is equipped with two
Carbonoxide solenoid valve 16.Aeration tank 11 gives three 1 oxygens of breeding device, carbon dioxide tank 14 respectively by three oxygen channels 12
Give three breeding devices 1 for carbon dioxide respectively by three carbon dioxide conduits 15.
The culture medium feed unit includes culture tank 17 and three culture medium pipelines 18, and each culture medium pipeline 18 connects
Culture tank 17 and a breeding device 1 are connect, each culture medium pipeline 18 is equipped with culture medium solenoid valve 19 and culture medium pump 20.Training
Base tank 17 is supported by three culture medium pipelines 18 respectively to three breeding devices 1 for culture medium.
The controller is equipped with for input information and shows the touch screen of information.
The light compensating apparatus 4, optical sensor 5, pH value sensor 6, dissolved oxygen sensor 7, temperature sensor 8, liquid level pass
Sensor 9, biosensor 10, circulation solenoid valve 2, water pump 3, oxygen solenoid valve 13, carbon dioxide solenoid valve 16, culture medium electromagnetism
Valve 19, culture medium pump 20, touch screen are electrically connected with the controller respectively.
Microorganism in the present embodiment includes but not limited to haematococcus pluvialis, and the culture of haematococcus pluvialis needs suitable ring
Border, culture medium is that configured in advance is good in the present embodiment, and the temperature of culture medium is 22 °~25 °, the pH value of culture medium is 7.0~
8.0.User is put into the microorganism liquid of certain microbial species amount in the first breeding device 111, and be put by touch screen input
Microbial species amount, the microbial species amount of the present embodiment are the volume of microorganism liquid, and required culture is calculated according to microbial species amount
Matrix accumulates, and to obtain microorganism liquid and the mixed culturing liquid total volume of culture medium, microorganism liquid is usually put into culture medium
It is as inoculated with, inoculum concentration is excessive or too small, can influence the growth of microorganism, crosses conference and causes dissolved oxygen insufficient, too small to extend
Incubation time.Rule of thumb, inoculum concentration is advisable for 10%~20%, and the inoculum concentration of the present embodiment is 10%.Microbial species amount is
1L, the then culturing liquid after being inoculated with are then 10L, and level threshold can be calculated as H1 in conjunction with the parameter of the first breeding device 111.
111 corresponding culture medium solenoid valve 19 of the first breeding device of controller control is opened and 20 work of culture medium pump, in culture tank 17
Culture medium culture medium pump 20 under the action of be transported to the first breeding device 111, the liquid level sensor 9 in the first breeding device 111
Level value is acquired, when level value reaches H1, control culture medium solenoid valve 19 is closed and culture medium pump 20 is stopped.Culturing liquid
It is formed, haematococcus pluvialis starts to cultivate in culture medium.
First day of culture can with stuffiness, additional illumination is not provided, give haematococcus pluvialis is certain to shake down
Time, start within second day to the first breeding device 111 provide additional illumination and input oxygen.The first breeding device of the present embodiment
111, the second breeding device 112 and third breeding device 113 correspond to inoculation growth period, fast growing period and the induction period of microorganism, because
This first breeding device 111 and the second breeding device 112 are mainly used for the growth to haematococcus pluvialis, and third breeding device 113 is mainly used
In the induction with haematococcus pluvialis, and the suitable optical density of growth phase is 800 μ E/m2.s~1500 μ E/m2.s.Microorganism
The oxygen dissolved optimum range of entire incubator is 45%~55%.
The light compensating apparatus 4 that controller controls the first breeding device 111 works, and is that the culturing liquid in breeding device 1 carries out light filling, when
Optical density is higher than 1500 μ E/m2When .s, control light compensating apparatus 4 is stopped, and stops carrying out light filling to culturing liquid；When optical density is low
In 800 μ E/m2.s, control light compensating apparatus 4 works again, to make the culturing liquid in the first breeding device 111 be maintained at 800 μ E/
m2.s~1500 μ E/m2.s in optical density threshold range.
111 corresponding oxygen solenoid valve 13 of the first breeding device of controller control is opened, and the oxygen in aeration tank 11 is transported to
In first breeding device 111, dissolved oxygen sensor 7 acquires the dissolved oxygen in the first breeding device 111, is controlled when dissolved oxygen is higher than 55%
Oxygen solenoid valve 13 is closed, and when dissolved oxygen is less than 45%, oxygen solenoid valve 13 is again turned on, to make the first breeding device 111
Interior culturing liquid is maintained in 45%~55% oxygen threshold range.
The liquid temperature threshold range of the present embodiment is 22 °~25 °, if room temperature is too low, liquid temperature under the influence of the external world
It can reduce, thus in liquid temperature threshold range of the present embodiment in order to make liquid holding, then need to keep room temperature in a certain range,
The temperature of culturing liquid in 8 collection bag of temperature sensor in first breeding device 111, when the liquid temperature value of acquisition is below or above liquid temperature
When threshold range, control room conditioning adjusts room temperature, and then controls liquid temperature.
Microorganism during growth, can absorb the chemical substance in culture medium, and pH value can change.The present embodiment is most suitable
Suitable PH threshold ranges be 7.0~8.0, therefore when the pH value of culturing liquid in the first breeding device 111 it is high 8.0 when, control first support
111 corresponding carbon dioxide solenoid valve 16 of device opening is grown, the carbon dioxide in carbon dioxide tank 14 is transported in this breeding device 1,
The pH value in culturing liquid is continuously decreased, when pH value is less than 7.0, control carbon dioxide solenoid valve 16 is closed, and stops conveying dioxy
Change carbon, to make the culturing liquid in the first breeding device 111 be maintained in 45%~55% oxygen threshold range.
In the first stage of inoculation growth, the biosensor 10 in the first breeding device 111 acquires the microorganism of culturing liquid
Amount, when microbial biomass is more than concentration threshold 2.0 × 104When a/mL, the first breeding device 111 due to volumetric constraint, microbial biomass compared with
Height can not provide preferable growing environment for microorganism.Controller control the first breeding device 111 and the second breeding device 112 it
Between circulation solenoid valve 2 open and water pump 3 works, the culturing liquid in the first breeding device 111 is transported to the under the action of water pump 3
Two breeding devices 112,112 volume of the second breeding device is twice of 111 volume of the first breeding device in the present embodiment.
After culturing liquid enters the second breeding device 112, this stage breeding liquid total volume is calculated according to microbial biomass, according to this rank
Level threshold can be calculated as H2 in conjunction with the parameter of the second breeding device 112 in section culturing liquid total volume.Controller control the
Two breeding devices, 112 corresponding culture medium solenoid valve 19 is opened and 20 work of culture medium pump, and the culture medium in culture tank 17 is being trained
It supports under the action of base pumps 20 and is transported to the second breeding device 112, the liquid level sensor 9 in the second breeding device 112 acquires level value, when
When level value reaches H2, control culture medium solenoid valve 19 is closed and culture medium pump 20 is stopped, and microorganism enters fast-growth
Second stage.This stage control optical density, oxygen dissolved, temperature, the method for pH value are identical with the first stage.When the second breeding device
Microbial biomass in 112 is more than concentration threshold 4.0 × 104When a/mL, the concentration of microorganism changes little, fast growing period
Terminate.Controller controls the opening of circulation solenoid valve 2 and the work of water pump 3 between the second breeding device 112 and third breeding device 113,
Culturing liquid in second breeding device 112 is transported to third breeding device 113 under the action of water pump 3, and third cultivates in the present embodiment
113 the second breeding device of volume ratio of device, 112 volume it is big.
After culturing liquid enters third breeding device 113, into the phase III of induction, a small amount of culture medium only need to be supplemented, this
The cell in stage is in stationary state, and cell volume becomes larger, and after cell wall obviously becomes, a small amount of cell interior even occurs red
Astaxanthin particle needs to induce astaxanthin under high light intensity at this time.Therefore the optical density threshold range in this stage is different from first
Stage and second stage, it is more much higher than preceding two stage optical density threshold range, to make microorganism quickly produce shrimp blueness
Element.When microbial biomass reaches ripe threshold value, then microculture is ripe.
The data information in each stage in the present embodiment can be checked that controller can be by each stage by touch screen
Data information be sent to the equipment such as long-range server, terminal, facilitate user to grasp the culture situation of microorganism at any time.
Total described, the present embodiment controls microorganism by the various data informations of the microorganism growing environment of acquisition
Growing environment improves the efficiency of micro-organisms, moreover it is possible to which remote monitoring is micro- to create optimum growing environment for microorganism
The growing state of biology.
Embodiment two：
A kind of method that microorganism is cultivated based on bioreactor is present embodiments provided, is suitable for described in embodiment one
Bioreactor, as shown in figure 5, including the following steps：
S1 calculates the total volume after mixed culture medium according to the microbial species amount being initially added in breeding device 1, according to totality
Product control culture medium feed unit conveys culture medium into this breeding device 1.The S1 is specially：
The microbial species amount for being initially put into the microorganism in breeding device 1 is obtained by touch screen；
The culturing liquid total volume after mixed culture medium is calculated according to microbial species amount, calculating this according to culturing liquid total volume supports
Grow the level threshold in device 1；
It controls 1 corresponding culture medium solenoid valve 19 of this breeding device opening and culture medium pump 20 works, in culture tank 17
Culture medium is transported in this breeding device 1, when the level value of 1 corresponding liquid level sensor 9 of this breeding device acquisition reaches level threshold
When, control culture medium solenoid valve 19 is closed and culture medium pump 20 is stopped, and the culturing liquid after mixed culture medium starts to cultivate micro-
Biology.
S2 obtains the microbial biomass that the environmental data and biosensor 10 of each sensor acquisition in this breeding device 1 acquire；
4 light filling of light compensating apparatus, control air supply unit gas supply and control room conditioning, which are controlled, according to environmental data adjusts room temperature；When micro-
When biomass reaches given threshold, by control system automatic work, culturing liquid is transported in next breeding device 1 and continues to support
It grows, and controls culture medium feed unit and convey culture medium to next breeding device 1；Until microorganism is cultivated ripe.The S2 tools
Body is：
Optical sensor 5 acquires the OD value of culturing liquid in this breeding device 1, when OD value is less than optical density threshold value model
When enclosing, the work of 1 corresponding light compensating apparatus 4 of this breeding device is controlled, light filling is carried out to culturing liquid, when OD value is higher than flux density threshold
When being worth range, control light compensating apparatus 4 is stopped, and stops carrying out light filling to culturing liquid；
Dissolved oxygen sensor 7 acquires the oxygen dissolved of culturing liquid in this breeding device 1, when oxygen dissolved is less than oxygen threshold range, control
1 corresponding oxygen solenoid valve 13 of this breeding device opening is made, the oxygen in aeration tank 11 is transported in this breeding device 1, works as oxygen dissolved
When less than oxygen threshold range, control oxygen solenoid valve 13 is closed, and stops conveying oxygen；
PH sensor acquires the pH value of culturing liquid in this breeding device 1, when pH value is higher than PH threshold ranges, controls this cultivation
1 corresponding carbon dioxide solenoid valve 16 of device is opened, and the carbon dioxide in carbon dioxide tank 14 is transported in this breeding device 1, works as PH
When value is less than PH threshold ranges, control carbon dioxide solenoid valve 16 is closed, and transport of carbon dioxide is stopped；
Temperature sensor 8 acquires the liquid temperature value of culturing liquid in this breeding device 1, when liquid temperature value is below or above liquid temperature threshold value model
When enclosing, control room conditioning adjusts room temperature, and then controls liquid temperature.
Biosensor 10 acquires the microbial biomass of culturing liquid in this breeding device 1, when microbial biomass is more than concentration threshold,
It controls 1 corresponding circulation solenoid valve 2 of this breeding device opening and water pump 3 works, the culturing liquid in this breeding device 1 is transported to next
In breeding device 1, and this stage breeding liquid total volume is calculated according to microbial biomass, and is controlled and trained according to this stage breeding liquid total volume
It supports base tank 17 and conveys culture medium to next breeding device 1, continue microorganism and cultivated；
When microbial biomass reaches ripe threshold value, then microculture is ripe.
The present embodiment is set there are three breeding device 1, and three breeding devices 1 include the first breeding device 111, the second breeding device
112 and third breeding device 113, the microorganism cultivation liquid of different growing stage is housed, because with microorganism in each breeding device 1
Growth, the microbial biomass in later stage is more than the microbial biomass of early period, needs growing space and the culture medium of bigger, therefore latter supports
The volume for growing device 1 is more than the volume of previous breeding device 1, and three breeding devices 1 correspond to three growth periods of microorganism：Inoculation growth
Phase, fast growing period and induction period can also be divided into other several periods in practical applications.
In the present embodiment, cultivating for haematococcus pluvialis needs suitable environment, and culture medium is configured in advance in the present embodiment
Alright, the temperature of culture medium is 22 °~25 °, and the pH value of culture medium is 7.0~8.0.User is put into the first breeding device 111
The microorganism liquid of certain microbial species amount, and the microbial species amount being put into, the microbial species of the present embodiment are inputted by touch screen
Amount is the volume of microorganism liquid, required culture volume is calculated according to microbial species amount, to obtain microorganism liquid and culture
The mixed culturing liquid total volume of base, it is to be inoculated with that microorganism liquid, which is usually put into culture medium, and inoculum concentration is excessive or too small, can
The growth of microorganism is influenced, conference is crossed and causes dissolved oxygen insufficient, it is too small to extend incubation time.Rule of thumb, inoculum concentration 10%
~20% is advisable, and the inoculum concentration of the present embodiment is 10%.Microbial species amount is 1L, then the culturing liquid after being inoculated with is then 10L, then is tied
Level threshold can be calculated as H1 by closing the parameter of the first breeding device 111.Controller controls the 111 corresponding training of the first breeding device
20 work of 19 opening of base solenoid valve and culture medium pump is supported, the culture medium in culture tank 17 conveys under the action of culture medium pump 20
To the first breeding device 111, the liquid level sensor 9 in the first breeding device 111 acquires level value, when level value reaches H1, control
Culture medium solenoid valve 19 is closed and culture medium pump 20 is stopped.Culturing liquid is formed, and haematococcus pluvialis starts to train in culture medium
It supports.
First day of culture can with stuffiness, additional illumination is not provided, give haematococcus pluvialis is certain to shake down
Time, start within second day to the first breeding device 111 provide additional illumination and input oxygen.The first breeding device of the present embodiment
111, the second breeding device 112 and third breeding device 113 correspond to inoculation growth period, fast growing period and the induction period of microorganism, because
This first breeding device 111 and the second breeding device 112 are mainly used for the growth to haematococcus pluvialis, and third breeding device 113 is mainly used
In the induction with haematococcus pluvialis, and the suitable optical density of growth phase is 800 μ E/m2.s~1500 μ E/m2.s.Microorganism
The oxygen dissolved optimum range of entire incubator is 45%~55%.
The light compensating apparatus 4 that controller controls the first breeding device 111 works, and is that the culturing liquid in breeding device 1 carries out light filling, when
Optical density is higher than 1500 μ E/m2When .s, control light compensating apparatus 4 is stopped, and stops carrying out light filling to culturing liquid；When optical density is low
In 800 μ E/m2.s, control light compensating apparatus 4 works again, to make the culturing liquid in the first breeding device 111 be maintained at 800 μ E/
m2.s~1500 μ E/m2.s in optical density threshold range.
111 corresponding oxygen solenoid valve 13 of the first breeding device of controller control is opened, and the oxygen in aeration tank 11 is transported to
In first breeding device 111, dissolved oxygen sensor 7 acquires the dissolved oxygen in the first breeding device 111, is controlled when dissolved oxygen is higher than 55%
Oxygen solenoid valve 13 is closed, and when dissolved oxygen is less than 45%, oxygen solenoid valve 13 is again turned on, to make the first breeding device 111
Interior culturing liquid is maintained in 45%~55% oxygen threshold range.
The liquid temperature threshold range of the present embodiment is 22 °~25 °, if room temperature is too low, liquid temperature under the influence of the external world
It can reduce, thus in liquid temperature threshold range of the present embodiment in order to make liquid holding, then need to keep room temperature in a certain range,
The temperature of culturing liquid in 8 collection bag of temperature sensor in first breeding device 111, when the liquid temperature value of acquisition is below or above liquid temperature
When threshold range, control room conditioning adjusts room temperature, and then controls liquid temperature.
Microorganism during growth, can absorb the chemical substance in culture medium, and pH value can change.The present embodiment is most suitable
Suitable PH threshold ranges be 7.0~8.0, therefore when the pH value of culturing liquid in the first breeding device 111 it is high 8.0 when, control first support
111 corresponding carbon dioxide solenoid valve 16 of device opening is grown, the carbon dioxide in carbon dioxide tank 14 is transported in this breeding device 1,
The pH value in culturing liquid is continuously decreased, when pH value is less than 7.0, control carbon dioxide solenoid valve 16 is closed, and stops conveying dioxy
Change carbon, to make the culturing liquid in the first breeding device 111 be maintained in 45%~55% oxygen threshold range.
In the first stage of inoculation growth, the biosensor 10 in the first breeding device 111 acquires the microorganism of culturing liquid
Amount, when microbial biomass is more than concentration threshold 1.0 × 104When a/mL, the first breeding device 111 due to volumetric constraint, microbial biomass compared with
Height can not provide preferable growing environment for microorganism.Controller control the first breeding device 111 and the second breeding device 112 it
Between circulation solenoid valve 2 open and water pump 3 works, the culturing liquid in the first breeding device 111 is transported to the under the action of water pump 3
Two breeding devices 112,112 volume of the second breeding device is twice of 111 volume of the first breeding device in the present embodiment.
After culturing liquid enters the second breeding device 112, this stage breeding liquid total volume is calculated according to microbial biomass, according to this rank
Level threshold can be calculated as H2 in conjunction with the parameter of the second breeding device 112 in section culturing liquid total volume.Controller control the
Two breeding devices, 112 corresponding culture medium solenoid valve 19 is opened and 20 work of culture medium pump, and the culture medium in culture tank 17 is being trained
It supports under the action of base pumps 20 and is transported to the second breeding device 112, the liquid level sensor 9 in the second breeding device 112 acquires level value, when
When level value reaches H2, control culture medium solenoid valve 19 is closed and culture medium pump 20 is stopped, and microorganism enters fast-growth
Second stage.This stage control optical density, oxygen dissolved, temperature, the method for pH value are identical with the first stage.When the second breeding device
Microbial biomass in 112 is more than concentration threshold 4.0 × 104When a/mL, the concentration of microorganism changes little, fast growing period
Terminate.Controller controls the opening of circulation solenoid valve 2 and the work of water pump 3 between the second breeding device 112 and third breeding device 113,
Culturing liquid in second breeding device 112 is transported to third breeding device 113 under the action of water pump 3, and third cultivates in the present embodiment
113 the second breeding device of volume ratio of device, 112 volume it is big.
After culturing liquid enters third breeding device 113, into the phase III of induction, a small amount of culture medium only need to be supplemented, this
The cell in stage is in stationary state, and cell volume becomes larger, and after cell wall obviously becomes, a small amount of cell interior even occurs red
Astaxanthin particle needs to induce astaxanthin under high light intensity at this time.Therefore the optical density threshold range in this stage is different from first
Stage and second stage, it is more much higher than preceding two stage optical density threshold range, to make microorganism quickly produce shrimp blueness
Element.When microbial biomass reaches ripe threshold value, then microculture is ripe.
The data information in each stage in the present embodiment can be checked that controller can be by each stage by touch screen
Data information be sent to the equipment such as long-range server, terminal, facilitate user to grasp the culture situation of microorganism at any time.
Total described, the present embodiment controls microorganism by the various data informations of the microorganism growing environment of acquisition
Growing environment improves the efficiency of micro-organisms, moreover it is possible to which remote monitoring is micro- to create optimum growing environment for microorganism
The growing state of biology.
In several embodiments provided herein, it should be understood that described method, it can be by others side
Formula is realized.Embodiment of the method described above is only schematical, for example, the division of the method, only one kind are patrolled
Volume function divides, and there may be another division manner when realization.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, it will be understood by those of ordinary skill in the art that：Its according to
So can with technical scheme described in the above embodiments is modified, either to which part or all technical features into
Row equivalent replacement；And these modifications or replacements, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme should all cover in the claim of the present invention and the range of specification.
Claims (10)
1. a kind of bioreactor, which is characterized in that including cultivation collecting unit, air supply unit, culture medium feed unit with
And the controller that can be communicated with external equipment；
Described several breeding devices for cultivating collecting unit and including transparent, which passes sequentially through circulation pipeline and is connected, adjacent
Circulation pipeline between two breeding devices is equipped with circulation solenoid valve and water pump；
Each breeding device is equipped with light compensating apparatus；
It is equipped in each breeding device：Optical sensor for perceiving intensity of illumination in bag；For detecting culture medium in breeding device
The pH value sensor of pH value；Dissolved oxygen sensor for detecting culture medium oxyty in breeding device；For detecting in breeding device
The temperature sensor of culture medium temperature；Liquid level sensor for detecting culture medium liquid level in breeding device；With for detecting biology
The biosensor of density；
The air supply unit includes being arranged in breeding device for exposing the aeration tank into oxygen, several oxygen hoses into breeding device
Road is arranged in breeding device for exposing carbon dioxide tank and several carbon dioxide conduits into carbon dioxide into breeding device,
Every oxygen channel connects aeration tank, and every oxygen channel is equipped with oxygen solenoid valve, and every carbon dioxide conduit connects dioxy
Change canister, every carbon dioxide conduit is equipped with carbon dioxide solenoid valve；
The culture medium feed unit includes culture tank and multiple culture medium pipelines, and each culture medium pipeline connects culture tank
With a breeding device, each culture medium pipeline is equipped with culture medium solenoid valve and culture medium pumps；
The controller is equipped with for input information and shows the touch screen of information；
The light compensating apparatus, optical sensor, pH value sensor, dissolved oxygen sensor, temperature sensor, liquid level sensor, biology
Sensor, circulation solenoid valve, water pump, oxygen solenoid valve, carbon dioxide solenoid valve, culture medium solenoid valve, culture medium pump, touch screen
It is electrically connected with the controller respectively.
2. bioreactor according to claim 1, which is characterized in that the breeding device lower part is equipped with connection flue
The liquid outlet of the oxygen inlet in road, the carbon dioxide inlet and connection flow tube road of connection carbon dioxide conduit, the breeding device
Top is equipped with the culture medium mouth of connection culture medium pipeline, the inlet in connection flow tube road and the exhaust outlet for exhaust.
3. bioreactor according to claim 2, which is characterized in that the light compensating apparatus include outer light compensating apparatus and
The side of interior light compensating apparatus, the breeding device is uniformly provided with vertical multiple outer light compensating apparatus, the location mid-shaft of the breeding device
Accommodating cavity equipped with upper end opening, the accommodating cavity is interior to be equipped with vertical interior light compensating apparatus.
4. bioreactor according to claim 3, which is characterized in that the outer light compensating apparatus includes outer light filling holder
The LED light bar being connect with outer light filling holder；
The outer light filling holder includes top plate and bottom plate and the side plate that is connected between top plate and bottom plate, and the side plate is in evagination
Cylinder, and the inner surface of the side plate is provided with reflective layer, and the both ends of the LED light bar are separately fixed at top plate and bottom plate
On.
5. bioreactor according to claim 3, which is characterized in that the interior light compensating apparatus includes the interior of cylinder
Light filling holder and the LED light bar being connect with interior light filling holder；
The interior light filling holder includes upper plate, the cycle side plate of lower plate and connection between the upper and lower plates, the LED light bar
Both ends be separately fixed at the center of upper plate and lower plate.
6. bioreactor according to claim 3, which is characterized in that the breeding device include successively sequential communication and
Gradually increased first breeding device ... N breeding devices, each breeding device are interior equipped with suitable for the micro- of different growing stage for volume
Biological cultivation liquid.
7. a kind of method cultivating microorganism based on bioreactor, is suitable for claim 1-6 any one of them photoproduction
Object reactor, which is characterized in that include the following steps：
S1 calculates the total volume after mixed culture medium according to the microbial species amount being initially added in breeding device, according to total volume control
Culture medium feed unit processed conveys culture medium into this breeding device；
S2 obtains the microbial biomass of the environmental data and biosensor acquisition of each sensor acquisition in this breeding device；According to ring
Border data control light compensating apparatus light filling, control air supply unit gas supply and control room conditioning adjust room temperature；When microbial biomass reaches
When to given threshold, by control system automatic work, culturing liquid is transported in next breeding device and continues to cultivate, and control training
It supports base feed unit and conveys culture medium to next breeding device；Until microorganism is cultivated ripe.
8. the method according to claim 7 for cultivating microorganism based on bioreactor, which is characterized in that the cultivation
Before device includes the first breeding device ... N breeding devices, the first breeding device to N breeding devices, and the volume of latter breeding device is more than
The volume of one breeding device；
The microorganism cultivation liquid of different growing stage is housed, the culturing liquid of different growing stage has difference in each breeding device
Optical density threshold range.
9. the method according to claim 8 for cultivating microorganism based on bioreactor, which is characterized in that the S1 tools
Body is：
The microbial species amount for being initially put into the microorganism in breeding device is obtained by touch screen；
The culturing liquid total volume after mixed culture medium is calculated according to microbial species amount, this breeding device is calculated according to culturing liquid total volume
Interior level threshold；
The corresponding culture medium solenoid valve opening of this breeding device and culture medium pump work are controlled, the culture medium in culture tank is transported to
In this breeding device, when the level value of the corresponding liquid level sensor acquisition of this breeding device reaches level threshold, control culture medium electricity
Magnet valve is closed and culture medium pump is stopped, and the culturing liquid after mixed culture medium starts to cultivate microorganism.
10. the method according to claim 9 for cultivating microorganism based on bioreactor, which is characterized in that the S2
Specially：
Optical sensor acquires the OD value of culturing liquid in this breeding device, when OD value is less than optical density threshold range,
The corresponding light compensating apparatus work of this breeding device is controlled, light filling is carried out to culturing liquid, when OD value is higher than optical density threshold range
When, control light compensating apparatus is stopped, and stops carrying out light filling to culturing liquid；
Dissolved oxygen sensor acquires the oxygen dissolved of culturing liquid in this breeding device, and when oxygen dissolved is less than oxygen threshold range, control is originally supported
It grows the corresponding oxygen solenoid valve of device to open, the oxygen in aeration tank is transported in this breeding device, when oxygen dissolved is less than oxygen threshold value model
When enclosing, control oxygen solenoid valve is closed, and stops conveying oxygen；
PH sensor acquires the pH value of culturing liquid in this breeding device, when pH value is higher than PH threshold ranges, controls this breeding device pair
The carbon dioxide solenoid valve answered is opened, and the carbon dioxide in carbon dioxide tank is transported in this breeding device, when pH value is less than PH thresholds
When being worth range, control carbon dioxide solenoid valve is closed, and transport of carbon dioxide is stopped；
Temperature sensor acquires the liquid temperature value of culturing liquid in this breeding device, when liquid temperature value is below or above liquid temperature threshold range,
It controls room conditioning and adjusts room temperature, and then control liquid temperature；
Biosensor acquires the microbial biomass of culturing liquid in this breeding device, and when microbial biomass is more than concentration threshold, control is originally
The corresponding circulation solenoid valve of breeding device is opened and pump working, and the culturing liquid in this breeding device is transported in next breeding device,
And this stage breeding liquid total volume is calculated according to microbial biomass, and it is downward according to this stage breeding liquid total volume control culture tank
One breeding device conveys culture medium, continues microorganism and is cultivated；
When microbial biomass reaches ripe threshold value, then microculture is ripe.
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| EP4389869A1 (en) * | 2022-12-22 | 2024-06-26 | AT-Solid GmbH | Photobioreactor for production of organisms that process photosynthesis |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2748464Y (en) * | 2004-05-31 | 2005-12-28 | 中国科学院过程工程研究所 | Air-lift type photo-bioreactor |
| WO2007011343A1 (en) * | 2005-07-18 | 2007-01-25 | Greenfuel Technologies Corporation | Photobioreactor and process for biomass production and mitigation of pollutants in flue gases |
| CN203890350U (en) * | 2014-06-12 | 2014-10-22 | 黑龙江省能源环境研究院 | Integrated culture device for oil-producing microalgae |
| CN105087371A (en) * | 2014-05-04 | 2015-11-25 | 北京工商大学 | Photobioreactor for automatically culturing microalgae |
| WO2016089302A1 (en) * | 2014-12-02 | 2016-06-09 | Agronosis Pte Ltd | Photobioreactor |
| CN205953993U (en) * | 2016-08-22 | 2017-02-15 | 上海海洋大学 | Smooth autotrophy that little algae of continuous culture was used is established ties and is expanded major connector |
| US20170321181A1 (en) * | 2016-05-09 | 2017-11-09 | Global Algae Innovations, Inc. | Biological and algae harvesting and cultivation systems and methods |
-
2018
- 2018-05-22 CN CN201810492502.9A patent/CN108641946A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2748464Y (en) * | 2004-05-31 | 2005-12-28 | 中国科学院过程工程研究所 | Air-lift type photo-bioreactor |
| WO2007011343A1 (en) * | 2005-07-18 | 2007-01-25 | Greenfuel Technologies Corporation | Photobioreactor and process for biomass production and mitigation of pollutants in flue gases |
| CN105087371A (en) * | 2014-05-04 | 2015-11-25 | 北京工商大学 | Photobioreactor for automatically culturing microalgae |
| CN203890350U (en) * | 2014-06-12 | 2014-10-22 | 黑龙江省能源环境研究院 | Integrated culture device for oil-producing microalgae |
| WO2016089302A1 (en) * | 2014-12-02 | 2016-06-09 | Agronosis Pte Ltd | Photobioreactor |
| US20170321181A1 (en) * | 2016-05-09 | 2017-11-09 | Global Algae Innovations, Inc. | Biological and algae harvesting and cultivation systems and methods |
| CN205953993U (en) * | 2016-08-22 | 2017-02-15 | 上海海洋大学 | Smooth autotrophy that little algae of continuous culture was used is established ties and is expanded major connector |
Non-Patent Citations (1)
| Title |
|---|
| 邓祥元主编: "《应用微藻生物学》", 30 November 2016, 海洋出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4389869A1 (en) * | 2022-12-22 | 2024-06-26 | AT-Solid GmbH | Photobioreactor for production of organisms that process photosynthesis |
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